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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_F01
         (653 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)             180   7e-46
SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)                     176   1e-44
SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)             153   1e-37
SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44)            140   1e-33
SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.)              79   2e-15
SB_23775| Best HMM Match : Tropomyosin (HMM E-Value=0)                 32   0.35 
SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   1.4  
SB_42068| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.5  
SB_41093| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   5.8  
SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)                     28   7.6  

>SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 309

 Score =  180 bits (439), Expect = 7e-46
 Identities = 95/156 (60%), Positives = 108/156 (69%), Gaps = 2/156 (1%)
 Frame = +2

Query: 188 MAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKE 367
           MA AMKE TE    L  EERNLLSVAYKNVVGA+RSSWRVISSIEQK EGSERK+Q  + 
Sbjct: 1   MAKAMKEATEISETLEQEERNLLSVAYKNVVGAKRSSWRVISSIEQKLEGSERKKQNTET 60

Query: 368 YXVKVQKELXEICYDVLGLLDKHLIPKASNPXSKVFYLKMKGXYYRYLAXXATGXTXHSV 547
           Y   ++ EL E+C  VL LL+  LIP A +  SKVFYLKMKG YYRY    A       V
Sbjct: 61  YRQTIENELNEVCETVLKLLESKLIPNAQSTESKVFYLKMKGDYYRYEGEVAGADRRREV 120

Query: 548 VQDSXKAYQDAFEISK--AKMQPTHPITLGLALNFS 649
           VQ + KAY +A EI++   K+ PT PI LGLALNFS
Sbjct: 121 VQKAMKAYSEAQEIAEKDPKLPPTDPIRLGLALNFS 156



 Score = 37.1 bits (82), Expect = 0.012
 Identities = 19/39 (48%), Positives = 28/39 (71%), Gaps = 3/39 (7%)
 Frame = +2

Query: 542 SVVQDSXKAYQDAFEISKA---KMQPTHPITLGLALNFS 649
           +VV+ + +AY++A E ++    K+ PT PI LGLALNFS
Sbjct: 207 TVVEKALQAYKEAKEAAETGDGKLAPTDPIRLGLALNFS 245


>SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)
          Length = 248

 Score =  176 bits (428), Expect = 1e-44
 Identities = 93/184 (50%), Positives = 120/184 (65%), Gaps = 3/184 (1%)
 Frame = +2

Query: 107 PSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGA 286
           P+      +EEL+  AK+AEQAERYDDM  AM  VT+ G  L++EERNLLSVAYKNVVGA
Sbjct: 3   PNFVSKCSREELIHLAKMAEQAERYDDMVNAMSAVTKEGKPLNDEERNLLSVAYKNVVGA 62

Query: 287 RRSSWRVISSIEQKTEGSERKQQMAKEYXVKVQKELXEICYDVLGLLDKHLIPKAS---N 457
           RRSSWRVISS+EQK    E    + K+Y   +  EL   C +VL +L+ +L+       N
Sbjct: 63  RRSSWRVISSMEQK--APEEMAALTKKYREDITNELNGKCAEVLDILENYLLKDGQDDIN 120

Query: 458 PXSKVFYLKMKGXYYRYLAXXATGXTXHSVVQDSXKAYQDAFEISKAKMQPTHPITLGLA 637
             +KVFYLKM+G Y+RYL   A G +    ++ S +AY+DA      ++ P+HPI LGLA
Sbjct: 121 TEAKVFYLKMRGDYHRYLVEVAEGDSRKENIEKSREAYKDA-SAKAEELSPSHPIRLGLA 179

Query: 638 LNFS 649
           LNFS
Sbjct: 180 LNFS 183


>SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 219

 Score =  153 bits (371), Expect = 1e-37
 Identities = 77/137 (56%), Positives = 101/137 (73%)
 Frame = +2

Query: 119 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSS 298
           M V +E L+  AKL+EQ +RYD+MA  MKEV+E   +LS EERNLLSV+YKN+VG RRSS
Sbjct: 80  MMVVRETLIYNAKLSEQCDRYDEMAKIMKEVSEKYPKLSKEERNLLSVSYKNIVGQRRSS 139

Query: 299 WRVISSIEQKTEGSERKQQMAKEYXVKVQKELXEICYDVLGLLDKHLIPKASNPXSKVFY 478
           WRVISSIE+KT  S     + K+Y   ++KEL ++C +VLG+L++ LIP A +  +KVFY
Sbjct: 140 WRVISSIEEKTAESS-SLAIVKKYKACIEKELKDLCKEVLGILER-LIPGAEDEENKVFY 197

Query: 479 LKMKGXYYRYLAXXATG 529
            K+KG YYRYLA  + G
Sbjct: 198 FKLKGDYYRYLAEFSHG 214


>SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44)
          Length = 251

 Score =  140 bits (338), Expect = 1e-33
 Identities = 72/123 (58%), Positives = 90/123 (73%), Gaps = 3/123 (2%)
 Frame = +2

Query: 128 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRV 307
           DKEE V  AKLAEQAERYDDM  +MKEV + G ELS E+RNLLSVAYKNV+GARR+SWR+
Sbjct: 3   DKEEHVYMAKLAEQAERYDDMVNSMKEVAKMGTELSTEDRNLLSVAYKNVIGARRASWRI 62

Query: 308 ISSIEQKTE--GSE-RKQQMAKEYXVKVQKELXEICYDVLGLLDKHLIPKASNPXSKVFY 478
           I+SIEQK E  G +  K +M + Y   +++EL  IC ++L LLD  LI  + +  SKVFY
Sbjct: 63  ITSIEQKEESKGEDMAKLEMIRNYRKTIEEELKTICGEILSLLDDSLIKNSQSEESKVFY 122

Query: 479 LKM 487
            K+
Sbjct: 123 NKI 125


>SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 607

 Score = 79.4 bits (187), Expect = 2e-15
 Identities = 42/102 (41%), Positives = 61/102 (59%), Gaps = 2/102 (1%)
 Frame = +2

Query: 119 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSS 298
           M   + ELVQ AKLAEQ ER++D+   MK+  E    L+ E RNLLSV YKNVVG++R +
Sbjct: 186 MQDSRNELVQLAKLAEQTERFEDVILYMKKAIEINPSLNKEHRNLLSVGYKNVVGSKRFA 245

Query: 299 WRVISSIEQKTEGSERKQQMAK--EYXVKVQKELXEICYDVL 418
           WR +     ++    R  Q+    +Y  K++ EL  +C ++L
Sbjct: 246 WRHLHHDALQSGRYIRDSQLKGIIKYKEKIEMELKTLCREIL 287


>SB_23775| Best HMM Match : Tropomyosin (HMM E-Value=0)
          Length = 442

 Score = 32.3 bits (70), Expect = 0.35
 Identities = 22/86 (25%), Positives = 40/86 (46%)
 Frame = +2

Query: 131 KEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVI 310
           KE+    AK  ++ ER +  A   K+  E   +   EE++ L    K     R+    + 
Sbjct: 339 KEKERLEAKQKKEQERLEKQAEKEKKEKERLEKKQREEKDRLEKKEKKEEEKRKKEEEIN 398

Query: 311 SSIEQKTEGSERKQQMAKEYXVKVQK 388
           + IE+K +  E+K+Q  +E   K ++
Sbjct: 399 AKIEEKKKREEKKKQEEEEKMKKKEQ 424


>SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 994

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 16/51 (31%), Positives = 28/51 (54%)
 Frame = +2

Query: 95  ISPLPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEER 247
           +SPLPS+      +E+V   KLAE+ E  ++++      T+   E + EE+
Sbjct: 643 VSPLPSTATEDQMQEVVDSNKLAEKKEVTEEVSPVKPLSTKESKENALEEK 693


>SB_42068| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 3367

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 27/121 (22%), Positives = 55/121 (45%), Gaps = 12/121 (9%)
 Frame = +2

Query: 116  TMSVDKEELVQRAKLAEQAERY---DDMAAAMKEVTETGVELSNE-ERNLLSVAYKNVVG 283
            ++S+  +EL  +  LA Q  +    D   A  K+VT   ++ + E  R    +   +   
Sbjct: 3123 SLSLKSQELEAKNALANQKLKQMVKDQQEAEKKKVTSMEIQTTIETHRKQTIIEMVSCAA 3182

Query: 284  ARRSSWR--------VISSIEQKTEGSERKQQMAKEYXVKVQKELXEICYDVLGLLDKHL 439
              R S++        + SS++++T+  + KQQ   +   +V+  + E    V G+  +HL
Sbjct: 3183 CLRKSYQESAFLDLLITSSLQKQTKQIKEKQQAVMKDLAQVEPAVDEARQAVKGIKKQHL 3242

Query: 440  I 442
            +
Sbjct: 3243 V 3243


>SB_41093| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 586

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 25/100 (25%), Positives = 44/100 (44%), Gaps = 6/100 (6%)
 Frame = +2

Query: 110 SSTMSVDKEELVQRAK-LAEQAERYDDMAAAMKEVTETGVELS-NEERNLLSVAY----K 271
           SS++    E  +   K  +E+ E+     A  KE  E  ++ + +EER   +  Y    K
Sbjct: 359 SSSLREKNEACLSECKETSEEIEQLKVDLAKYKEAAEKDLDKALSEERAKFTEEYAKLEK 418

Query: 272 NVVGARRSSWRVISSIEQKTEGSERKQQMAKEYXVKVQKE 391
               ARR   + + S+ Q      R+++  KE   + QK+
Sbjct: 419 QFNDARREHMKAVVSLRQAERQLAREKEKGKEQIAQKQKD 458


>SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)
          Length = 783

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +2

Query: 347 KQQMAKEYXVKVQKELXEICYDVLGLLDKHLIPK 448
           K++M ++Y  K++KE+    Y+++  L K ++ K
Sbjct: 292 KEEMKEKYDGKIEKEMSGAIYEIISRLMKAVVGK 325


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,425,142
Number of Sequences: 59808
Number of extensions: 361175
Number of successful extensions: 952
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 882
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 946
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1669334250
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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