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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0020
         (596 letters)

Database: mosquito 
           2352 sequences; 563,979 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha ...    29   0.15 
AY027891-1|AAK15783.1|  801|Anopheles gambiae collagen IV alpha ...    25   2.5  
DQ303468-1|ABC18327.1| 1115|Anopheles gambiae putative methopren...    24   3.2  
M93690-2|AAA29363.1| 1212|Anopheles gambiae unknown protein.           24   4.3  
AJ439398-8|CAD28131.1| 1283|Anopheles gambiae putative different...    24   4.3  
M93689-1|AAA29368.1|  442|Anopheles gambiae protein ( Anopheles ...    23   9.9  
AJ439353-10|CAD27932.1| 3325|Anopheles gambiae F25C8.3 protein p...    23   9.9  

>AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha 1
           chain protein.
          Length = 1024

 Score = 28.7 bits (61), Expect = 0.15
 Identities = 21/67 (31%), Positives = 25/67 (37%), Gaps = 5/67 (7%)
 Frame = +2

Query: 227 GSKRAPGGTAALGNPPGTPRPGFS-----MSRRGANGLNIIPPPKGENGSRSEWPTLRPG 391
           G K  PG   A+G P     PG S         G  GL  +P P G NG       + P 
Sbjct: 476 GDKGEPGFPGAIGRPGKVGVPGLSGEAGAKGEMGIQGLPGLPGPAGLNGLPGMKGDMGPL 535

Query: 392 GTAHRSC 412
           G    +C
Sbjct: 536 GEKGDAC 542



 Score = 27.1 bits (57), Expect = 0.46
 Identities = 16/48 (33%), Positives = 21/48 (43%), Gaps = 2/48 (4%)
 Frame = +2

Query: 227 GSKRAPGGTAALGN--PPGTPRPGFSMSRRGANGLNIIPPPKGENGSR 364
           G +  PG     G+  PPG   P      R  +GLN +  P+G  G R
Sbjct: 618 GQRGLPGPQGEKGDQGPPGFIGPKGDKGERDRDGLNGLNGPQGMKGDR 665



 Score = 26.6 bits (56), Expect = 0.61
 Identities = 17/55 (30%), Positives = 21/55 (38%)
 Frame = +2

Query: 227 GSKRAPGGTAALGNPPGTPRPGFSMSRRGANGLNIIPPPKGENGSRSEWPTLRPG 391
           G +  PG     G P      G      G +GLN  P  KG+ G   + P  R G
Sbjct: 663 GDRGMPGLEGVAGLPGMVGEKG-DRGLPGMSGLNGAPGEKGQKGETPQLPPQRKG 716



 Score = 26.2 bits (55), Expect = 0.80
 Identities = 20/63 (31%), Positives = 24/63 (38%), Gaps = 2/63 (3%)
 Frame = +2

Query: 224 NGSKRAPGGTAALGNPPGTPRPGFSMSRRGANGLNII--PPPKGENGSRSEWPTLRPGGT 397
           N     P G       PG P    S   +G  GL+++  P PKG  G R   P    GG 
Sbjct: 67  NSGPVGPPGAPGRDGMPGAPGLPGSKGVKGDPGLSMVGPPGPKGNPGLRG--PKGERGGM 124

Query: 398 AHR 406
             R
Sbjct: 125 GDR 127



 Score = 24.6 bits (51), Expect = 2.5
 Identities = 11/24 (45%), Positives = 13/24 (54%)
 Frame = -3

Query: 312 PRRDIENPGLGVPGGLPRAAVPPG 241
           P+ D   PGL  P G+P A   PG
Sbjct: 726 PKGDKGLPGLAGPAGIPGAPGAPG 749


>AY027891-1|AAK15783.1|  801|Anopheles gambiae collagen IV alpha 1
           chain precursor protein.
          Length = 801

 Score = 24.6 bits (51), Expect = 2.5
 Identities = 16/48 (33%), Positives = 19/48 (39%), Gaps = 2/48 (4%)
 Frame = +2

Query: 227 GSKRAPG--GTAALGNPPGTPRPGFSMSRRGANGLNIIPPPKGENGSR 364
           G K  PG  G   L  P G   P     R G  G+   P  +GE G +
Sbjct: 226 GQKGEPGNDGLEGLPGPQGEVGPRGFPGRPGEKGVPGTPGVRGERGDK 273



 Score = 24.6 bits (51), Expect = 2.5
 Identities = 14/44 (31%), Positives = 17/44 (38%)
 Frame = +2

Query: 227 GSKRAPGGTAALGNPPGTPRPGFSMSRRGANGLNIIPPPKGENG 358
           G   APGG       PG   P      +G  G++     KGE G
Sbjct: 398 GPAGAPGGGEGRPGAPGPKGPRGYEGPQGPKGMDGFDGEKGERG 441



 Score = 24.2 bits (50), Expect = 3.2
 Identities = 18/52 (34%), Positives = 21/52 (40%), Gaps = 2/52 (3%)
 Frame = +2

Query: 227 GSKRAPGGTAALGNP--PGTPRPGFSMSRRGANGLNIIPPPKGENGSRSEWP 376
           G+   PG     G P   GTP        +G  GL   P P GE G  +E P
Sbjct: 604 GASGVPGERGYPGMPGEDGTPGLRGEPGPKGEPGLLGPPGPSGEPGRDAEIP 655



 Score = 23.8 bits (49), Expect = 4.3
 Identities = 18/53 (33%), Positives = 26/53 (49%), Gaps = 3/53 (5%)
 Frame = +2

Query: 242 PGGTAALGNPPGTPRPGFSMSRRGA---NGLNIIPPPKGENGSRSEWPTLRPG 391
           PG     G P   P   ++  ++G    +GL  +P P+GE G R  +P  RPG
Sbjct: 207 PGTKGEKGEPARHPE-NYNKGQKGEPGNDGLEGLPGPQGEVGPRG-FPG-RPG 256



 Score = 22.6 bits (46), Expect = 9.9
 Identities = 14/41 (34%), Positives = 17/41 (41%)
 Frame = +2

Query: 248 GTAALGNPPGTPRPGFSMSRRGANGLNIIPPPKGENGSRSE 370
           G A  G P     PG     +G  G   +P  KGE G + E
Sbjct: 536 GDAKEGRPGAPGLPGRD-GEKGEPGRPGLPGAKGERGLKGE 575


>DQ303468-1|ABC18327.1| 1115|Anopheles gambiae putative
           methoprene-tolerant protein protein.
          Length = 1115

 Score = 24.2 bits (50), Expect = 3.2
 Identities = 11/20 (55%), Positives = 11/20 (55%)
 Frame = -3

Query: 396 VPPGRNVGHSDLDPFSPFGG 337
           VPP  N  H    PFSP GG
Sbjct: 827 VPPLPNSQHYFTQPFSPSGG 846


>M93690-2|AAA29363.1| 1212|Anopheles gambiae unknown protein.
          Length = 1212

 Score = 23.8 bits (49), Expect = 4.3
 Identities = 7/21 (33%), Positives = 12/21 (57%)
 Frame = +1

Query: 262  WQSTWNTQAWVLNVSTWSKRV 324
            WQS W+ +A     + W+ R+
Sbjct: 944  WQSQWDAEADTSRYTRWTHRI 964


>AJ439398-8|CAD28131.1| 1283|Anopheles gambiae putative
           differentiation regulator protein.
          Length = 1283

 Score = 23.8 bits (49), Expect = 4.3
 Identities = 10/17 (58%), Positives = 10/17 (58%)
 Frame = +2

Query: 236 RAPGGTAALGNPPGTPR 286
           R PG  AA G PP T R
Sbjct: 908 RGPGAAAATGPPPPTHR 924


>M93689-1|AAA29368.1|  442|Anopheles gambiae protein ( Anopheles
           gambiae T1 retroposon. ).
          Length = 442

 Score = 22.6 bits (46), Expect = 9.9
 Identities = 10/20 (50%), Positives = 12/20 (60%)
 Frame = -3

Query: 279 VPGGLPRAAVPPGARFDPFA 220
           V  GLP+  +   ARFDP A
Sbjct: 331 VDRGLPKQRIHERARFDPSA 350


>AJ439353-10|CAD27932.1| 3325|Anopheles gambiae F25C8.3 protein
            protein.
          Length = 3325

 Score = 22.6 bits (46), Expect = 9.9
 Identities = 8/22 (36%), Positives = 15/22 (68%)
 Frame = +2

Query: 74   CVIVYITLSHILQNTDYMNIFS 139
            C++ Y+T + I   +++ NIFS
Sbjct: 2237 CIMRYVTATFINAASNFKNIFS 2258


  Database: mosquito
    Posted date:  Oct 23, 2007  1:18 PM
  Number of letters in database: 563,979
  Number of sequences in database:  2352
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 615,458
Number of Sequences: 2352
Number of extensions: 13653
Number of successful extensions: 44
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 30
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 43
length of database: 563,979
effective HSP length: 61
effective length of database: 420,507
effective search space used: 57609459
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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