Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20193
(817 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 112 3e-25
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 108 5e-24
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 106 2e-23
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 101 4e-22
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 68 7e-12
At4g28000.1 68417.m04016 AAA-type ATPase family protein contains... 30 1.6
At1g58400.1 68414.m06644 disease resistance protein (CC-NBS-LRR ... 30 1.6
At1g18265.1 68414.m02278 expressed protein 29 2.8
At5g42940.1 68418.m05235 zinc finger (C3HC4-type RING finger) fa... 29 3.7
At5g05730.1 68418.m00630 anthranilate synthase, alpha subunit, c... 28 6.4
At1g54860.1 68414.m06263 expressed protein 28 6.4
At3g51760.1 68416.m05676 hypothetical protein hypothetical prote... 28 8.5
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 112 bits (269), Expect = 3e-25
Identities = 48/89 (53%), Positives = 65/89 (73%)
Frame = +2
Query: 245 EGHRKFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAA 424
E H KF+ GDSYI+L+TT+ +L DIHYW+G++S+QDE+GA A++TV LD GG A
Sbjct: 37 ESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLGKDSSQDEAGAVAVMTVELDSALGGRA 96
Query: 425 VQHRETMGHESALFLSYFQTPLTYLEGGI 511
VQ+RE GHE+ FLSYF+ + EGG+
Sbjct: 97 VQYREVQGHETEKFLSYFKPCIIPQEGGV 125
Score = 57.6 bits (133), Expect = 9e-09
Identities = 38/120 (31%), Positives = 56/120 (46%)
Frame = +1
Query: 400 GRQVRGRRGPAPRDHGSRKCFVPIIFPDTLDLFGGRDPSGFNHVVTNAGAQKRMFQVKGK 579
GR V+ R F P I P G SGFNHV Q R++ KGK
Sbjct: 94 GRAVQYREVQGHETEKFLSYFKPCIIPQE-----GGVASGFNHVKPEEH-QTRLYICKGK 147
Query: 580 RDVRVRQVDPQIASMNKGDCFVLSLDNDIYVFVGEKAKNVEKLKAISFANQVRDQDHHGR 759
VRV++V +++N D F+L ++ I+ F G K+ E+ KA+ ++D H G+
Sbjct: 148 HVVRVKEVPFVRSTLNHEDVFILDTESKIFQFSGSKSSIQERAKALEVVQYIKDTYHDGK 207
Score = 35.5 bits (78), Expect = 0.042
Identities = 12/27 (44%), Positives = 20/27 (74%)
Frame = +3
Query: 168 AFANVGRTAGVQIWRIQNFEPIPVAQK 248
A G+ +G++IWRI+NF+P+ V Q+
Sbjct: 11 ALQGAGQKSGIEIWRIENFKPVTVPQE 37
Score = 34.7 bits (76), Expect = 0.074
Identities = 22/85 (25%), Positives = 39/85 (45%)
Frame = +2
Query: 257 KFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAAVQHR 436
KFY GD Y IL+ + + + W G++S +++ +A L + + Q R
Sbjct: 416 KFYSGDCY-ILQYSYPGEDREEHLVGTWFGKQSVEEDRASAISLANKMVESMKFVPAQAR 474
Query: 437 ETMGHESALFLSYFQTPLTYLEGGI 511
G E F Q+ +T+ +GG+
Sbjct: 475 INEGKEPIQFFVIMQSFITF-KGGV 498
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 108 bits (259), Expect = 5e-24
Identities = 48/87 (55%), Positives = 63/87 (72%)
Frame = +2
Query: 251 HRKFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAAVQ 430
H KFY GD+YI+L+TT + +DIH+WIG++++QDE+G AA+ TV LD GG AVQ
Sbjct: 37 HGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIGKDTSQDEAGTAAVKTVELDAVLGGRAVQ 96
Query: 431 HRETMGHESALFLSYFQTPLTYLEGGI 511
HRE GHES FLSYF+ + LEGG+
Sbjct: 97 HREIQGHESDKFLSYFKPCIIPLEGGV 123
Score = 54.4 bits (125), Expect = 9e-08
Identities = 35/105 (33%), Positives = 53/105 (50%)
Frame = +1
Query: 442 HGSRKCFVPIIFPDTLDLFGGRDPSGFNHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIAS 621
H S K F+ P + L GG SGF V + R++ KGKR +R++QV +S
Sbjct: 103 HESDK-FLSYFKPCIIPLEGGV-ASGFKTVEEEV-FETRLYTCKGKRAIRLKQVPFARSS 159
Query: 622 MNKGDCFVLSLDNDIYVFVGEKAKNVEKLKAISFANQVRDQDHHG 756
+N D F+L + IY F G + E+ KA+ ++D+ H G
Sbjct: 160 LNHDDVFILDTEEKIYQFNGANSNIQERAKALEVVQYLKDKYHEG 204
Score = 48.0 bits (109), Expect = 7e-06
Identities = 28/101 (27%), Positives = 45/101 (44%)
Frame = +2
Query: 257 KFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAAVQHR 436
K Y GD Y++L T + + W G++S ++ A L + + G VQ R
Sbjct: 412 KLYSGDCYLVLYTYHSGERKDEYFLSCWFGKKSIPEDQDTAIRLANTMSNSLKGRPVQGR 471
Query: 437 ETMGHESALFLSYFQTPLTYLEGGIRPVLTTSSRTREPRNE 559
G E F++ FQ P+ L+GG+ +S E +E
Sbjct: 472 IYEGKEPPQFVALFQ-PMVVLKGGLSSGYKSSMGESESTDE 511
Score = 39.1 bits (87), Expect = 0.003
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +3
Query: 165 PAFANVGRTAGVQIWRIQNFEPIPVAQKD 251
PAF G+ G +IWRI+NFE +PV + +
Sbjct: 8 PAFQGAGQKPGTEIWRIENFEAVPVPKSE 36
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 106 bits (254), Expect = 2e-23
Identities = 47/87 (54%), Positives = 63/87 (72%)
Frame = +2
Query: 251 HRKFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAAVQ 430
H KFY GD+YI+L+TT + +DIH+WIG++++QDE+G AA+ TV LD GG AVQ
Sbjct: 39 HGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIGKDTSQDEAGTAAVKTVELDAALGGRAVQ 98
Query: 431 HRETMGHESALFLSYFQTPLTYLEGGI 511
+RE GHES FLSYF+ + LEGG+
Sbjct: 99 YREIQGHESDKFLSYFKPCIIPLEGGV 125
Score = 48.0 bits (109), Expect = 7e-06
Identities = 34/105 (32%), Positives = 50/105 (47%)
Frame = +1
Query: 442 HGSRKCFVPIIFPDTLDLFGGRDPSGFNHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIAS 621
H S K F+ P + L GG SGF + R++ KGKR V ++QV +S
Sbjct: 105 HESDK-FLSYFKPCIIPLEGGV-ASGFKKPEEEE-FETRLYTCKGKRAVHLKQVPFARSS 161
Query: 622 MNKGDCFVLSLDNDIYVFVGEKAKNVEKLKAISFANQVRDQDHHG 756
+N D F+L IY F G + E+ KA+ ++D+ H G
Sbjct: 162 LNHDDVFILDTKEKIYQFNGANSNIQERAKALVVIQYLKDKFHEG 206
Score = 46.4 bits (105), Expect = 2e-05
Identities = 28/99 (28%), Positives = 45/99 (45%), Gaps = 1/99 (1%)
Frame = +2
Query: 218 ELRTNTRCSEGH-RKFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAILTV 394
+ + T S+ H K Y GD Y++L T + + W G+ S Q++ A L
Sbjct: 400 DANSKTVLSKDHVGKLYSGDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQETAVRLAS 459
Query: 395 GLDDKFGGAAVQHRETMGHESALFLSYFQTPLTYLEGGI 511
+ + G VQ R G E F++ FQ + L+GG+
Sbjct: 460 TMTNSLKGRPVQARIFEGKEPPQFVALFQ-HMVVLKGGL 497
Score = 44.0 bits (99), Expect = 1e-04
Identities = 16/29 (55%), Positives = 22/29 (75%)
Frame = +3
Query: 165 PAFANVGRTAGVQIWRIQNFEPIPVAQKD 251
PAF VG+ G +IWRI+NFEP+PV + +
Sbjct: 10 PAFQGVGQKPGTEIWRIENFEPVPVPKSE 38
Score = 27.9 bits (59), Expect = 8.5
Identities = 13/51 (25%), Positives = 26/51 (50%)
Frame = +1
Query: 574 GKRDVRVRQVDPQIASMNKGDCFVLSLDNDIYVFVGEKAKNVEKLKAISFA 726
G + + QV+ S+N DCF+L ++++VG + + ++ A A
Sbjct: 529 GVHNNKALQVEAVATSLNSYDCFLLQSGTSMFLWVGNHSTHEQQELAAKVA 579
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 101 bits (243), Expect = 4e-22
Identities = 45/85 (52%), Positives = 61/85 (71%)
Frame = +2
Query: 257 KFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAAVQHR 436
KF+ GDSYI+L+TT+ L DIHYW+G++++QDE+G AA+ TV LD GG AVQ+R
Sbjct: 41 KFFTGDSYIVLKTTALKTGALRHDIHYWLGKDTSQDEAGTAAVKTVELDAALGGRAVQYR 100
Query: 437 ETMGHESALFLSYFQTPLTYLEGGI 511
E GHE+ FLSYF+ + EGG+
Sbjct: 101 EVQGHETEKFLSYFKPCIIPQEGGV 125
Score = 50.4 bits (115), Expect = 1e-06
Identities = 35/119 (29%), Positives = 51/119 (42%)
Frame = +1
Query: 400 GRQVRGRRGPAPRDHGSRKCFVPIIFPDTLDLFGGRDPSGFNHVVTNAGAQKRMFQVKGK 579
GR V+ R F P I P G SGF HVV R+F +GK
Sbjct: 94 GRAVQYREVQGHETEKFLSYFKPCIIPQE-----GGVASGFKHVVAEEHIT-RLFVCRGK 147
Query: 580 RDVRVRQVDPQIASMNKGDCFVLSLDNDIYVFVGEKAKNVEKLKAISFANQVRDQDHHG 756
V V++V +S+N D ++L + I+ F G + E+ KA+ ++D H G
Sbjct: 148 HVVHVKEVPFARSSLNHDDIYILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDG 206
Score = 41.1 bits (92), Expect = 9e-04
Identities = 15/30 (50%), Positives = 21/30 (70%)
Frame = +3
Query: 165 PAFANVGRTAGVQIWRIQNFEPIPVAQKDI 254
PAF G+ AG++IWRI+NF P P+ + I
Sbjct: 10 PAFQGAGQKAGIEIWRIENFIPTPIPKSSI 39
Score = 39.9 bits (89), Expect = 0.002
Identities = 29/119 (24%), Positives = 52/119 (43%)
Frame = +2
Query: 155 QSPSGFCQCRQNSRCANMENTELRTNTRCSEGHRKFYKGDSYIILRTTSDSRNNLSWDIH 334
+ P F C N + + N + +T + ++ H KFY GD Y + + + I
Sbjct: 382 EEPQVFIDCTGNLQVWRV-NGQAKTLLQAAD-HSKFYSGDCY-VFQYSYPGEEKEEVLIG 438
Query: 335 YWIGRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLTYLEGGI 511
W G++S ++E G+A + + + Q R G E F Q+ + + +GGI
Sbjct: 439 TWFGKQSVEEERGSAVSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVF-KGGI 496
Score = 29.5 bits (63), Expect = 2.8
Identities = 12/39 (30%), Positives = 22/39 (56%)
Frame = +1
Query: 565 QVKGKRDVRVRQVDPQIASMNKGDCFVLSLDNDIYVFVG 681
Q G +++ QVDP AS+N ++L D+ ++ + G
Sbjct: 525 QGSGPENMQAIQVDPVAASLNSSYYYILHNDSSVFTWAG 563
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 68.1 bits (159), Expect = 7e-12
Identities = 32/87 (36%), Positives = 51/87 (58%)
Frame = +2
Query: 257 KFYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAAVQHR 436
KF+ G++Y++LRT + +DIHYW+G ++ + +S A+ + LD G VQ+R
Sbjct: 41 KFHSGNAYLVLRTFLRKIESPQYDIHYWLGIDANEVDSILASDKALDLDAALGCCTVQYR 100
Query: 437 ETMGHESALFLSYFQTPLTYLEGGIRP 517
E G E+ FLSYF+ + +EG P
Sbjct: 101 EVQGQETEKFLSYFKPCIIPVEGKYSP 127
Score = 47.2 bits (107), Expect = 1e-05
Identities = 22/70 (31%), Positives = 37/70 (52%)
Frame = +1
Query: 550 QKRMFQVKGKRDVRVRQVDPQIASMNKGDCFVLSLDNDIYVFVGEKAKNVEKLKAISFAN 729
Q + + KG VRV++V +S+N D F+L + +++F G + EK KA+
Sbjct: 137 QVTLLRCKGDHVVRVKEVPFLRSSLNHDDVFILDTASKVFLFAGCNSSTQEKAKAMEVVE 196
Query: 730 QVRDQDHHGR 759
++D H GR
Sbjct: 197 YIKDNKHDGR 206
Score = 30.3 bits (65), Expect = 1.6
Identities = 24/114 (21%), Positives = 46/114 (40%)
Frame = +2
Query: 170 FCQCRQNSRCANMENTELRTNTRCSEGHRKFYKGDSYIILRTTSDSRNNLSWDIHYWIGR 349
+ CR N + ++ ++ + K + GD Y++ + + ++ WIG
Sbjct: 384 YTNCRDNLKVWRVDGDDV--SLLSIPDQTKLFTGDCYLV-QYKYTYKERTEHLLYVWIGC 440
Query: 350 ESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLTYLEGGI 511
ES Q + A + G +V G+E + F FQ+ L +GG+
Sbjct: 441 ESIQQDRADAITNASAIVGTTKGESVLCHIYQGNEPSRFFPMFQS-LVVFKGGL 493
>At4g28000.1 68417.m04016 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family
Length = 726
Score = 30.3 bits (65), Expect = 1.6
Identities = 20/48 (41%), Positives = 25/48 (52%)
Frame = +2
Query: 452 ESALFLSYFQTPLTYLEGGIRPVLTTSSRTREPRNECSRSKGSEMSAL 595
+S F FQ LT L G PVL SR EP ++C + G +SAL
Sbjct: 217 QSERFYKLFQRLLTKLSG---PVLVLGSRLLEPEDDC-QEVGEGISAL 260
>At1g58400.1 68414.m06644 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 900
Score = 30.3 bits (65), Expect = 1.6
Identities = 14/50 (28%), Positives = 21/50 (42%)
Frame = -3
Query: 650 LRTKQSPLFIEAICGSTCLTRTSRFPLTWNIRFWAPAFVTTWLKPDGSLP 501
L K+ L A CG ++ FP + W A W+ +GS+P
Sbjct: 789 LELKEVRLDFRAFCGKRMVSSDGGFPQLHRLYIWGLAEWEEWIVEEGSMP 838
>At1g18265.1 68414.m02278 expressed protein
Length = 280
Score = 29.5 bits (63), Expect = 2.8
Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 5/81 (6%)
Frame = +2
Query: 182 RQNSRCANMENTELRTNTRCSEGH-RKFYKGDSYIILRTTSDSRNNLSWD----IHYWIG 346
R++ C N N N S+ H IIL +SDS N LSW+ + G
Sbjct: 83 REHKDCNNNNNNNNNNNGFISKSHLADGLDQKKPIILHWSSDSTNRLSWENCDLLLLRDG 142
Query: 347 RESTQDESGAAAILTVGLDDK 409
+ Q+ A+ LD+K
Sbjct: 143 LDHIQNRENTVALSETELDEK 163
>At5g42940.1 68418.m05235 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 691
Score = 29.1 bits (62), Expect = 3.7
Identities = 16/40 (40%), Positives = 19/40 (47%), Gaps = 3/40 (7%)
Frame = +3
Query: 330 STTGSVGSRRKTSQEPPQSSPLAWTTSSGAP---RSSTER 440
S+ GS PP SS LAWT+ +P R TER
Sbjct: 409 SSVSRTGSTTSVPPPPPPSSNLAWTSYQNSPHYQRRRTER 448
>At5g05730.1 68418.m00630 anthranilate synthase, alpha subunit,
component I-1 (ASA1) identical to SP|P32068
Length = 595
Score = 28.3 bits (60), Expect = 6.4
Identities = 15/44 (34%), Positives = 24/44 (54%), Gaps = 5/44 (11%)
Frame = -3
Query: 542 AFVTTWLKPDGSLPPNRSRVSG-----NMIGTKHFRDPWSLGAG 426
A+V W++ DGSLP ++ +G N++ H +P L AG
Sbjct: 243 AYVIHWIRLDGSLPYEKAYSNGMQHLENLVAKLHDIEPPKLAAG 286
>At1g54860.1 68414.m06263 expressed protein
Length = 200
Score = 28.3 bits (60), Expect = 6.4
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -1
Query: 214 ILHICTPAVLPTLAKAGWTLALSRAFLSDSDVIG 113
IL +C P +PTLA +T +L+DS +G
Sbjct: 113 ILPVCIPNRIPTLALTNYTQTGYARYLNDSRYVG 146
>At3g51760.1 68416.m05676 hypothetical protein hypothetical protein
F17M19.12 - Arabidopsis thaliana, EMBL:AC021665
Length = 228
Score = 27.9 bits (59), Expect = 8.5
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +3
Query: 327 ISTTGSVGSRRKTSQEPPQSSPLAWTTSSGAPRSST 434
+ST V + Q P S P W T+ G P++S+
Sbjct: 26 LSTKRHVTQEKLPGQFPEASVPFCWETAPGMPKNSS 61
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,261,756
Number of Sequences: 28952
Number of extensions: 443304
Number of successful extensions: 1476
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1393
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1475
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1863090400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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