SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20173
         (728 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g34450.1 68417.m04896 coatomer gamma-2 subunit, putative / ga...    95   4e-20
At2g33330.1 68415.m04085 33 kDa secretory protein-related contai...    31   0.59 
At5g11490.1 68418.m01341 adaptin family protein similar to SP|Q9...    31   0.78 
At1g04520.1 68414.m00443 33 kDa secretory protein-related contai...    29   2.4  
At5g61980.1 68418.m07779 ARF GTPase-activating domain-containing...    29   3.2  
At4g18440.1 68417.m02736 adenylosuccinate lyase, putative / aden...    29   3.2  
At3g22990.1 68416.m02899 expressed protein                             29   3.2  
At4g28820.1 68417.m04120 zinc finger (HIT type) family protein c...    28   5.5  
At1g14970.1 68414.m01788 expressed protein contains Pfam PF03138...    28   5.5  

>At4g34450.1 68417.m04896 coatomer gamma-2 subunit, putative /
           gamma-2 coat protein, putative / gamma-2 COP, putative
           similar to SP|Q9UBF2 Coatomer gamma-2 subunit (Gamma-2
           coat protein) (Gamma-2 COP) {Homo sapiens}; contains
           Pfam profile: PF01602 Adaptin N terminal region
          Length = 886

 Score = 95.1 bits (226), Expect = 4e-20
 Identities = 47/68 (69%), Positives = 53/68 (77%)
 Frame = +3

Query: 255 RMVYLCIKELSKLAQDVIIVTSSLTKDMTGKEDLYRAAAIRALCSITDASMLQAIERYMK 434
           RMVYL IKELS  + +VIIVTSSL KDM  K D+YRA AIR LC I D ++L  IERY+K
Sbjct: 88  RMVYLIIKELSPSSDEVIIVTSSLMKDMNSKIDMYRANAIRVLCRIIDGTLLTQIERYLK 147

Query: 435 QAIVDKNP 458
           QAIVDKNP
Sbjct: 148 QAIVDKNP 155



 Score = 94.3 bits (224), Expect = 7e-20
 Identities = 43/82 (52%), Positives = 59/82 (71%)
 Frame = +1

Query: 10  LKRDVKDEDDNSSNPYQNLDKTIVLQETREFNQTLVIPRKCSLILTKILYLLNQGENFTT 189
           +K+D   +D+   +P+  ++K  VLQE R FN   V PR+CS ++TK+LYLLNQGE+FT 
Sbjct: 6   VKKDDDHDDELEYSPFMGIEKGAVLQEARVFNDPQVDPRRCSQVITKLLYLLNQGESFTK 65

Query: 190 QEATDAFFATTKLFQSKEIMLR 255
            EAT+ FF+ TKLFQSK+  LR
Sbjct: 66  VEATEVFFSVTKLFQSKDTGLR 87



 Score = 73.3 bits (172), Expect = 1e-13
 Identities = 32/69 (46%), Positives = 50/69 (72%)
 Frame = +2

Query: 509 PDLVRRWANEAQETINSDNAMVSYHALGVLVLSRRNDKLSTVKLVTRLSKSHIKSPYSLC 688
           P++V+RW+NE QE I S +A+V +HAL +L   R+ND+L+  KLV  L++  ++SP + C
Sbjct: 173 PEIVKRWSNEVQEGIQSRSALVQFHALALLHQIRQNDRLAVSKLVGSLTRGSVRSPLAQC 232

Query: 689 LLIRLAAQL 715
           LLIR  +Q+
Sbjct: 233 LLIRYTSQV 241


>At2g33330.1 68415.m04085 33 kDa secretory protein-related contains
           Pfam PF01657: Domain of unknown function, duplicated in
           33 KDa secretory proteins
          Length = 304

 Score = 31.5 bits (68), Expect = 0.59
 Identities = 15/37 (40%), Positives = 19/37 (51%)
 Frame = +2

Query: 362 GGRNKSVVQHHGRLYATSYRTIYETGDCGQEPGRQFC 472
           G     VVQ HG  YAT+Y ++Y  G C  + G   C
Sbjct: 170 GVMQNGVVQGHG-FYATTYESVYVLGQCEGDIGDSDC 205


>At5g11490.1 68418.m01341 adaptin family protein similar to
           SP|Q9WV76 Adapter-related protein complex 4 beta 1
           subunit (Beta subunit of AP- 4) {Mus musculus},
           beta-adaptin Drosophila 1 {Drosophila melanogaster}
           GI:434902; contains Pfam profile: PF01602 Adaptin N
           terminal region
          Length = 841

 Score = 31.1 bits (67), Expect = 0.78
 Identities = 16/68 (23%), Positives = 37/68 (54%), Gaps = 1/68 (1%)
 Frame = +3

Query: 255 RMVYLCIKELSKLAQDVIIVTSS-LTKDMTGKEDLYRAAAIRALCSITDASMLQAIERYM 431
           +M YL +   +K   D+ ++T + L +D   ++ + R  A+R+LCS+   ++++ +   +
Sbjct: 81  KMCYLYVGNYAKGNPDLSLLTINFLQRDCKDEDPMIRGLALRSLCSLRVPNLVEYLVGPL 140

Query: 432 KQAIVDKN 455
              + D N
Sbjct: 141 GSGLKDNN 148


>At1g04520.1 68414.m00443 33 kDa secretory protein-related contains
           Pfam PF01657: Domain of unknown function, duplicated in
           33 KDa secretory proteins
          Length = 307

 Score = 29.5 bits (63), Expect = 2.4
 Identities = 14/37 (37%), Positives = 18/37 (48%)
 Frame = +2

Query: 362 GGRNKSVVQHHGRLYATSYRTIYETGDCGQEPGRQFC 472
           G     VV  HG  YAT+Y ++Y  G C  + G   C
Sbjct: 168 GVMQNGVVSGHG-FYATTYESVYVLGQCEGDVGDTDC 203


>At5g61980.1 68418.m07779 ARF GTPase-activating domain-containing
           protein similar to GCN4-complementing protein (GCP1)
           GI:6465806 from [Arabidopsis thaliana]
          Length = 850

 Score = 29.1 bits (62), Expect = 3.2
 Identities = 18/41 (43%), Positives = 24/41 (58%)
 Frame = -2

Query: 430 IYRSIACSIEASVMLHNALIAAARYRSSLPVMSLVKEEVTM 308
           +YR I CS EA V   NAL   A Y  SLP+  +++ E T+
Sbjct: 686 VYRHIVCS-EADV---NALRGQASYTVSLPLSKMMQMEETL 722


>At4g18440.1 68417.m02736 adenylosuccinate lyase, putative /
           adenylosuccinase, putative similar to SP|P25739
           Adenylosuccinate lyase (EC 4.3.2.2) (Adenylosuccinase)
           {Escherichia coli}; contains Pfam profile PF00206: Lyase
          Length = 536

 Score = 29.1 bits (62), Expect = 3.2
 Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 5/48 (10%)
 Frame = -2

Query: 586 CVIRHHRIVRIDCFLSFISPSP-----NKIRAVEDK*SAETNAAELTA 458
           C+  HHR+ R+ C +S  + SP      K+ A++   S +   + LTA
Sbjct: 34  CLASHHRLPRVSCSVSTTTDSPKLVTSTKVTAMDGVSSRDLEMSNLTA 81


>At3g22990.1 68416.m02899 expressed protein
          Length = 460

 Score = 29.1 bits (62), Expect = 3.2
 Identities = 14/40 (35%), Positives = 22/40 (55%)
 Frame = +3

Query: 207 FLCDNKTVSVQRNHAARMVYLCIKELSKLAQDVIIVTSSL 326
           F+ DN+ V  Q  H    V+ CI +   + +D  +VT+SL
Sbjct: 224 FMPDNEVVMAQHRHCLETVFQCIHD--HMTEDEELVTNSL 261


>At4g28820.1 68417.m04120 zinc finger (HIT type) family protein
           contains Pfam profile: PF04438 HIT zinc finger
          Length = 173

 Score = 28.3 bits (60), Expect = 5.5
 Identities = 11/27 (40%), Positives = 18/27 (66%)
 Frame = +1

Query: 37  DNSSNPYQNLDKTIVLQETREFNQTLV 117
           D+SSNP Q LD+ + ++  REF   ++
Sbjct: 137 DSSSNPLQELDEAMGIEAFREFTDKIL 163


>At1g14970.1 68414.m01788 expressed protein contains Pfam PF03138:
           Plant protein family. The function of this family of
           plant proteins is unknown;  previously annotated as
           'auxin-independent growth promoter -related'  based on
           similarity to axi 1 protein (GB:X80301) (GI:559920) from
           [Nicotiana tabacum], which, due to scienitific fraud was
           retracted. Retraction in: Schell J. EMBO J 1999 May
           17;18(10):2908. PMID:10400497.
          Length = 562

 Score = 28.3 bits (60), Expect = 5.5
 Identities = 15/59 (25%), Positives = 32/59 (54%)
 Frame = -2

Query: 442 IACFIYRSIACSIEASVMLHNALIAAARYRSSLPVMSLVKEEVTMITSCANLLSSLMQR 266
           I+C ++   A S +AS ++H      + YRS   V + ++ E+    + A+ +S++ +R
Sbjct: 77  ISCMLFHLHAASFDASPIIHRRPAPGSVYRSP-QVYARLRGEIEADNTTADAISTIWKR 134


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,355,284
Number of Sequences: 28952
Number of extensions: 273570
Number of successful extensions: 747
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 719
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 747
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -