Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00089
(732 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g62820.1 68414.m07092 calmodulin, putative similar to calmodu... 34 0.11
At1g12310.1 68414.m01423 calmodulin, putative similar to calmodu... 34 0.11
At1g76040.2 68414.m08829 calcium-dependent protein kinase, putat... 31 0.79
At1g76040.1 68414.m08830 calcium-dependent protein kinase, putat... 31 0.79
At3g22930.1 68416.m02889 calmodulin, putative strong similarity ... 30 1.4
At5g37780.1 68418.m04549 calmodulin-1/4 (CAM1) identical to calm... 29 2.4
At5g21274.1 68418.m02533 calmodulin-6 (CAM6) identical to calmod... 29 2.4
At3g56800.1 68416.m06317 calmodulin-2/3/5 (CAM3) identical to ca... 29 2.4
At3g43810.1 68416.m04682 calmodulin-7 (CAM7) almost identical to... 29 2.4
At2g41110.1 68415.m05078 calmodulin-2/3/5 (CAM2) (CAL1) almost i... 29 2.4
At2g27030.3 68415.m03247 calmodulin-2/3/5 (CAM5) (TCH1) identica... 29 2.4
At2g27030.2 68415.m03246 calmodulin-2/3/5 (CAM5) (TCH1) identica... 29 2.4
At2g27030.1 68415.m03245 calmodulin-2/3/5 (CAM5) (TCH1) identica... 29 2.4
At1g66410.1 68414.m07542 calmodulin-1/4 (CAM4) identical to calm... 29 2.4
At4g36500.1 68417.m05182 expressed protein 29 3.2
At2g41100.2 68415.m05077 touch-responsive protein / calmodulin-r... 29 4.2
At4g35310.1 68417.m05019 calcium-dependent protein kinase, putat... 28 5.6
At3g54490.1 68416.m06029 eukaryotic rpb5 RNA polymerase subunit ... 28 5.6
At2g41100.1 68415.m05076 touch-responsive protein / calmodulin-r... 28 5.6
At4g14640.1 68417.m02252 calmodulin-8 (CAM8) identical to calmod... 28 7.3
At4g38230.1 68417.m05399 calcium-dependent protein kinase, putat... 27 9.7
At2g17290.1 68415.m01997 calcium-dependent protein kinase isofor... 27 9.7
>At1g62820.1 68414.m07092 calmodulin, putative similar to calmodulin
SP:P04465 from [Trypanosoma brucei gambiense]; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 148
Score = 33.9 bits (74), Expect = 0.11
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +2
Query: 182 EAFKKCDKEGTGFITAGDFRNIM 250
+AFK DKEGTGF+ D R+I+
Sbjct: 87 DAFKVLDKEGTGFVAVADLRHIL 109
>At1g12310.1 68414.m01423 calmodulin, putative similar to calmodulin
SP:P04465 from [Trypanosoma brucei gambiense]
Length = 148
Score = 33.9 bits (74), Expect = 0.11
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +2
Query: 182 EAFKKCDKEGTGFITAGDFRNIM 250
+AFK DKEGTGF+ D R+I+
Sbjct: 87 DAFKVLDKEGTGFVAVADLRHIL 109
>At1g76040.2 68414.m08829 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase GB:AAC25423 GI:3283996 [Nicotiana tabacum]
Length = 534
Score = 31.1 bits (67), Expect = 0.79
Identities = 28/89 (31%), Positives = 39/89 (43%), Gaps = 6/89 (6%)
Frame = +2
Query: 2 GLVAFDEFAEVMRKTALHKKLPFNMESTFVRLYFGKD--KKRLVTYPEFSQFLHDFH--- 166
G + FDE + LH+ ES +L D K + Y EF H
Sbjct: 403 GTITFDEL-----RNGLHRLGSKLTESEIKQLMEAADVDKSGTIDYIEFVTATMHRHRLE 457
Query: 167 -EEYGVEAFKKCDKEGTGFITAGDFRNIM 250
EE +EAFK DK+ +GFIT + ++ M
Sbjct: 458 KEENLIEAFKYFDKDRSGFITRDELKHSM 486
>At1g76040.1 68414.m08830 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase GB:AAC25423 GI:3283996 [Nicotiana tabacum]
Length = 323
Score = 31.1 bits (67), Expect = 0.79
Identities = 28/89 (31%), Positives = 39/89 (43%), Gaps = 6/89 (6%)
Frame = +2
Query: 2 GLVAFDEFAEVMRKTALHKKLPFNMESTFVRLYFGKD--KKRLVTYPEFSQFLHDFH--- 166
G + FDE + LH+ ES +L D K + Y EF H
Sbjct: 192 GTITFDEL-----RNGLHRLGSKLTESEIKQLMEAADVDKSGTIDYIEFVTATMHRHRLE 246
Query: 167 -EEYGVEAFKKCDKEGTGFITAGDFRNIM 250
EE +EAFK DK+ +GFIT + ++ M
Sbjct: 247 KEENLIEAFKYFDKDRSGFITRDELKHSM 275
>At3g22930.1 68416.m02889 calmodulin, putative strong similarity to
calmodulin 8 GI:5825600 from [Arabidopsis thaliana];
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 173
Score = 30.3 bits (65), Expect = 1.4
Identities = 23/89 (25%), Positives = 39/89 (43%), Gaps = 5/89 (5%)
Frame = +2
Query: 2 GLVAFDEFAEVMRKTALHKKLPFNMESTFVRLYFGKDKKRLVTYPEFSQFLHDFHEEYGV 181
G + DE A V+R + P E + D + + EF + + +E
Sbjct: 49 GCITADELATVIRSLDQN---PTEQELQDMITEIDSDGNGTIEFSEFLNLMANQLQETDA 105
Query: 182 -----EAFKKCDKEGTGFITAGDFRNIMM 253
EAFK DK+ G+I+A + R++M+
Sbjct: 106 DEELKEAFKVFDKDQNGYISASELRHVMI 134
>At5g37780.1 68418.m04549 calmodulin-1/4 (CAM1) identical to
calmodulin 4 [Arabidopsis thaliana] GI:16223, SP|P25854
Calmodulin-1/4 {Arabidopsis thaliana}
Length = 149
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 167 EEYGVEAFKKCDKEGTGFITAGDFRNIM 250
EE EAF+ DK+ GFI+A + R++M
Sbjct: 83 EEELKEAFRVFDKDQNGFISAAELRHVM 110
>At5g21274.1 68418.m02533 calmodulin-6 (CAM6) identical to
calmodulin-6 SP:Q03509 from [Arabidopsis thaliana];
contains Pfam profile: PF00036 EF hand
Length = 149
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 167 EEYGVEAFKKCDKEGTGFITAGDFRNIM 250
EE EAF+ DK+ GFI+A + R++M
Sbjct: 83 EEELKEAFRVFDKDQNGFISAAELRHVM 110
>At3g56800.1 68416.m06317 calmodulin-2/3/5 (CAM3) identical to
calmodulin GI:474183 from [Arabidopsis thaliana]; almost
identical to calmodulin-2/3/5 SP:P25069 [Arabidopsis
thaliana]
Length = 149
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 167 EEYGVEAFKKCDKEGTGFITAGDFRNIM 250
EE EAF+ DK+ GFI+A + R++M
Sbjct: 83 EEELKEAFRVFDKDQNGFISAAELRHVM 110
>At3g43810.1 68416.m04682 calmodulin-7 (CAM7) almost identical to
calmodulin GI:16227 from [Arabidopsis thaliana],
SP|P59220 Calmodulin-7 {Arabidopsis thaliana}
Length = 149
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 167 EEYGVEAFKKCDKEGTGFITAGDFRNIM 250
EE EAF+ DK+ GFI+A + R++M
Sbjct: 83 EEELKEAFRVFDKDQNGFISAAELRHVM 110
>At2g41110.1 68415.m05078 calmodulin-2/3/5 (CAM2) (CAL1) almost
identical to Calmodulin-2/3/5 SP:P25069 from
[Arabidopsis thaliana]
Length = 149
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 167 EEYGVEAFKKCDKEGTGFITAGDFRNIM 250
EE EAF+ DK+ GFI+A + R++M
Sbjct: 83 EEELKEAFRVFDKDQNGFISAAELRHVM 110
>At2g27030.3 68415.m03247 calmodulin-2/3/5 (CAM5) (TCH1) identical
to calmodulin GI:474183 from [Arabidopsis thaliana],
SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
Length = 181
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 167 EEYGVEAFKKCDKEGTGFITAGDFRNIM 250
EE EAF+ DK+ GFI+A + R++M
Sbjct: 83 EEELKEAFRVFDKDQNGFISAAELRHVM 110
>At2g27030.2 68415.m03246 calmodulin-2/3/5 (CAM5) (TCH1) identical
to calmodulin GI:474183 from [Arabidopsis thaliana],
SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
Length = 113
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 167 EEYGVEAFKKCDKEGTGFITAGDFRNIM 250
EE EAF+ DK+ GFI+A + R++M
Sbjct: 47 EEELKEAFRVFDKDQNGFISAAELRHVM 74
>At2g27030.1 68415.m03245 calmodulin-2/3/5 (CAM5) (TCH1) identical
to calmodulin GI:474183 from [Arabidopsis thaliana],
SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
Length = 149
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 167 EEYGVEAFKKCDKEGTGFITAGDFRNIM 250
EE EAF+ DK+ GFI+A + R++M
Sbjct: 83 EEELKEAFRVFDKDQNGFISAAELRHVM 110
>At1g66410.1 68414.m07542 calmodulin-1/4 (CAM4) identical to
calmodulin [Arabidopsis thaliana] GI:16223; nearly
identical to SP|P25854 Calmodulin-1/4 {Arabidopsis
thaliana}
Length = 149
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 167 EEYGVEAFKKCDKEGTGFITAGDFRNIM 250
EE EAF+ DK+ GFI+A + R++M
Sbjct: 83 EEELKEAFRVFDKDQNGFISAAELRHVM 110
>At4g36500.1 68417.m05182 expressed protein
Length = 122
Score = 29.1 bits (62), Expect = 3.2
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +2
Query: 95 LYFGKDKKRLVTYPEFSQFLHDFHE 169
L++GK KK T PEFS++L E
Sbjct: 82 LFYGKKKKSTTTKPEFSRYLEYLKE 106
>At2g41100.2 68415.m05077 touch-responsive protein /
calmodulin-related protein 3, touch-induced (TCH3)
identical to calmodulin-related protein 3, touch-induced
SP:P25071 from [Arabidopsis thaliana]
Length = 235
Score = 28.7 bits (61), Expect = 4.2
Identities = 25/83 (30%), Positives = 34/83 (40%)
Frame = +2
Query: 2 GLVAFDEFAEVMRKTALHKKLPFNMESTFVRLYFGKDKKRLVTYPEFSQFLHDFHEEYGV 181
G + F EF VM K H + P + KK +V Y + +F E
Sbjct: 62 GTIDFPEFLCVMAKNQGHDQAPRHT------------KKTMVDYQLTDDQILEFRE---- 105
Query: 182 EAFKKCDKEGTGFITAGDFRNIM 250
AF+ DK G G+IT + R M
Sbjct: 106 -AFRVFDKNGDGYITVNELRTTM 127
>At4g35310.1 68417.m05019 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calmodulin-domain protein
kinase CDPK isoform 6 [Arabidopsis thaliana]
gi|1399275|gb|AAB03246; contains protein kinase domain,
Pfam:PF00069; contains EF hand domain (calcium-binding
EF-hand), Pfam:PF00036, INTERPRO:IPR002048
Length = 556
Score = 28.3 bits (60), Expect = 5.6
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = +2
Query: 167 EEYGVEAFKKCDKEGTGFITAGDFR 241
EE+ V AF+ DK+G+GFIT + +
Sbjct: 472 EEHLVAAFQYFDKDGSGFITIDELQ 496
>At3g54490.1 68416.m06029 eukaryotic rpb5 RNA polymerase subunit
family protein similar to SP|P19388 DNA-directed RNA
polymerase II 23 kDa polypeptide (EC 2.7.7.6) {Homo
sapiens}; contains Pfam profiles PF03871: RNA polymerase
Rpb5 N-terminal domain, PF01191: RNA polymerase Rpb5
C-terminal domain
Length = 233
Score = 28.3 bits (60), Expect = 5.6
Identities = 19/51 (37%), Positives = 31/51 (60%), Gaps = 2/51 (3%)
Frame = +2
Query: 17 DEFAEVMRKTALH-KKLPFNMES-TFVRLYFGKDKKRLVTYPEFSQFLHDF 163
+E +++RK AL K+LP+ E +FVR Y+G KK++V + + DF
Sbjct: 177 EEKEQLLRKHALEDKQLPYLQEKDSFVR-YYGLKKKQVVKITYSKEPVGDF 226
>At2g41100.1 68415.m05076 touch-responsive protein /
calmodulin-related protein 3, touch-induced (TCH3)
identical to calmodulin-related protein 3, touch-induced
SP:P25071 from [Arabidopsis thaliana]
Length = 324
Score = 28.3 bits (60), Expect = 5.6
Identities = 24/83 (28%), Positives = 34/83 (40%)
Frame = +2
Query: 2 GLVAFDEFAEVMRKTALHKKLPFNMESTFVRLYFGKDKKRLVTYPEFSQFLHDFHEEYGV 181
G + F EF +M K H + P + KK +V Y + +F E
Sbjct: 151 GTIDFPEFLYLMAKNQGHDQAPRHT------------KKTMVDYQLTDDQILEFRE---- 194
Query: 182 EAFKKCDKEGTGFITAGDFRNIM 250
AF+ DK G G+IT + R M
Sbjct: 195 -AFRVFDKNGDGYITVNELRTTM 216
>At4g14640.1 68417.m02252 calmodulin-8 (CAM8) identical to
calmodulin 8 GI:5825600 from [Arabidopsis thaliana]
Length = 151
Score = 27.9 bits (59), Expect = 7.3
Identities = 23/90 (25%), Positives = 38/90 (42%), Gaps = 6/90 (6%)
Frame = +2
Query: 2 GLVAFDEFAEVMRKTALHKKLPFNMESTFVRLYFGKDKKRLVTYPEFSQFL------HDF 163
G + +E A V+R + P E + D + + EF + D
Sbjct: 27 GCITVEELATVIRSLDQN---PTEQELHDIITEIDSDSNGTIEFAEFLNLMAKKLQESDA 83
Query: 164 HEEYGVEAFKKCDKEGTGFITAGDFRNIMM 253
EE EAFK DK+ G+I+A + ++M+
Sbjct: 84 EEELK-EAFKVFDKDQNGYISASELSHVMI 112
>At4g38230.1 68417.m05399 calcium-dependent protein kinase, putative
/ CDPK, putative calmodulin-domain protein kinase CDPK
isoform 6 [Arabidopsis thaliana] gi|1399275|gb|AAB03246;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 340
Score = 27.5 bits (58), Expect = 9.7
Identities = 16/49 (32%), Positives = 28/49 (57%), Gaps = 4/49 (8%)
Frame = +2
Query: 110 DKKRLVTYPEF---SQFLHDFH-EEYGVEAFKKCDKEGTGFITAGDFRN 244
DK + Y EF + L+ EE+ + AF+ DK+G+G+IT + ++
Sbjct: 232 DKSGTIDYGEFIAATIHLNKLEREEHLLSAFRYFDKDGSGYITIDELQH 280
>At2g17290.1 68415.m01997 calcium-dependent protein kinase isoform 6
(CPK6) identical to calmodulin-domain protein kinase
CDPK isoform 6 [Arabidopsis thaliana]
gi|1399275|gb|AAB03246; contains protein kinase domain,
Pfam:PF00069; contains EF hand domain (calcium-binding
EF-hand), Pfam:PF00036, INTERPRO:IPR002048
Length = 544
Score = 27.5 bits (58), Expect = 9.7
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +2
Query: 167 EEYGVEAFKKCDKEGTGFITAGDFR 241
EE+ V AF+ DK+G+G+IT + +
Sbjct: 460 EEHLVSAFQYFDKDGSGYITIDELQ 484
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,316,637
Number of Sequences: 28952
Number of extensions: 350136
Number of successful extensions: 1176
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1176
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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