Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00068
(748 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 153 2e-37
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 153 2e-37
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 153 2e-37
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 153 2e-37
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 100 1e-21
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 94 8e-20
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 68 6e-12
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 66 3e-11
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 41 0.001
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 41 0.001
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 38 0.005
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 38 0.007
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 34 0.087
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 34 0.12
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 34 0.12
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 33 0.27
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.27
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.27
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 33 0.27
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 31 0.61
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 31 0.61
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 31 0.61
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 31 0.61
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 31 1.1
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 31 1.1
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 2.5
At1g47840.1 68414.m05325 hexokinase, putative similar to hexokin... 29 4.3
At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containi... 28 5.7
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 5.7
At3g18370.1 68416.m02336 C2 domain-containing protein contains P... 28 5.7
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 5.7
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 7.6
At5g06810.1 68418.m00770 mitochondrial transcription termination... 28 7.6
At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) / beta-fruc... 28 7.6
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 27 10.0
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 27 10.0
At5g02010.1 68418.m00120 expressed protein contains Pfam profile... 27 10.0
At3g05470.1 68416.m00599 formin homology 2 domain-containing pro... 27 10.0
At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha s... 27 10.0
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 153 bits (370), Expect = 2e-37
Identities = 71/85 (83%), Positives = 78/85 (91%)
Frame = +3
Query: 255 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 434
IDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQ
Sbjct: 73 IDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQ 132
Query: 435 TREHALLAFTLGVKQLIVGVNKMDS 509
TREHALLAFTLGVKQ+I NKMD+
Sbjct: 133 TREHALLAFTLGVKQMICCCNKMDA 157
Score = 138 bits (333), Expect = 5e-33
Identities = 65/72 (90%), Positives = 66/72 (91%)
Frame = +1
Query: 40 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 219
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 220 DKLKAERERGIT 255
DKLKAERERGIT
Sbjct: 61 DKLKAERERGIT 72
Score = 85.0 bits (201), Expect = 5e-17
Identities = 35/56 (62%), Positives = 45/56 (80%)
Frame = +2
Query: 509 TEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 676
T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 158 TTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 153 bits (370), Expect = 2e-37
Identities = 71/85 (83%), Positives = 78/85 (91%)
Frame = +3
Query: 255 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 434
IDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQ
Sbjct: 73 IDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQ 132
Query: 435 TREHALLAFTLGVKQLIVGVNKMDS 509
TREHALLAFTLGVKQ+I NKMD+
Sbjct: 133 TREHALLAFTLGVKQMICCCNKMDA 157
Score = 138 bits (333), Expect = 5e-33
Identities = 65/72 (90%), Positives = 66/72 (91%)
Frame = +1
Query: 40 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 219
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 220 DKLKAERERGIT 255
DKLKAERERGIT
Sbjct: 61 DKLKAERERGIT 72
Score = 85.0 bits (201), Expect = 5e-17
Identities = 35/56 (62%), Positives = 45/56 (80%)
Frame = +2
Query: 509 TEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 676
T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 158 TTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 153 bits (370), Expect = 2e-37
Identities = 71/85 (83%), Positives = 78/85 (91%)
Frame = +3
Query: 255 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 434
IDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQ
Sbjct: 73 IDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQ 132
Query: 435 TREHALLAFTLGVKQLIVGVNKMDS 509
TREHALLAFTLGVKQ+I NKMD+
Sbjct: 133 TREHALLAFTLGVKQMICCCNKMDA 157
Score = 138 bits (333), Expect = 5e-33
Identities = 65/72 (90%), Positives = 66/72 (91%)
Frame = +1
Query: 40 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 219
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 220 DKLKAERERGIT 255
DKLKAERERGIT
Sbjct: 61 DKLKAERERGIT 72
Score = 85.0 bits (201), Expect = 5e-17
Identities = 35/56 (62%), Positives = 45/56 (80%)
Frame = +2
Query: 509 TEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 676
T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 158 TTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 153 bits (370), Expect = 2e-37
Identities = 71/85 (83%), Positives = 78/85 (91%)
Frame = +3
Query: 255 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 434
IDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQ
Sbjct: 73 IDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQ 132
Query: 435 TREHALLAFTLGVKQLIVGVNKMDS 509
TREHALLAFTLGVKQ+I NKMD+
Sbjct: 133 TREHALLAFTLGVKQMICCCNKMDA 157
Score = 138 bits (333), Expect = 5e-33
Identities = 65/72 (90%), Positives = 66/72 (91%)
Frame = +1
Query: 40 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 219
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 220 DKLKAERERGIT 255
DKLKAERERGIT
Sbjct: 61 DKLKAERERGIT 72
Score = 85.0 bits (201), Expect = 5e-17
Identities = 35/56 (62%), Positives = 45/56 (80%)
Frame = +2
Query: 509 TEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 676
T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 158 TTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 100 bits (239), Expect = 1e-21
Identities = 46/84 (54%), Positives = 59/84 (70%)
Frame = +3
Query: 255 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 434
+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEFE G + GQ
Sbjct: 167 VEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEFETGYERGGQ 226
Query: 435 TREHALLAFTLGVKQLIVGVNKMD 506
TREH LA TLGV +LIV VNKMD
Sbjct: 227 TREHVQLAKTLGVSKLIVVVNKMD 250
Score = 70.9 bits (166), Expect = 8e-13
Identities = 30/69 (43%), Positives = 49/69 (71%)
Frame = +1
Query: 49 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 228
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 229 KAERERGIT 255
+ ER +G T
Sbjct: 158 EEERLKGKT 166
Score = 39.1 bits (87), Expect = 0.003
Identities = 20/55 (36%), Positives = 34/55 (61%), Gaps = 3/55 (5%)
Frame = +2
Query: 521 YSEPRFEEIKKEVSSYIKKIGYNPAA-VAFVPISGWHGDNMLEPSTK--MPWFKG 676
+S+ R++EI++++ ++K GYN V F+PISG G NM + + PW+ G
Sbjct: 256 WSKERYDEIEQKMVPFLKASGYNTKKDVVFLPISGLMGKNMDQRMGQEICPWWSG 310
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 94.3 bits (224), Expect = 8e-20
Identities = 43/96 (44%), Positives = 64/96 (66%), Gaps = 1/96 (1%)
Frame = +3
Query: 261 IALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK-NGQT 437
+A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEAG GQT
Sbjct: 307 VAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEAGFDNLKGQT 366
Query: 438 REHALLAFTLGVKQLIVGVNKMDSLNHHTVSPDLRK 545
REHA + GV+Q+IV +NKMD + + DL K
Sbjct: 367 REHARVLRGFGVEQVIVAINKMDIVGYSKERFDLIK 402
Score = 85.4 bits (202), Expect = 4e-17
Identities = 36/67 (53%), Positives = 50/67 (74%)
Frame = +1
Query: 55 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 234
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 235 ERERGIT 255
ERERGIT
Sbjct: 298 ERERGIT 304
Score = 35.5 bits (78), Expect = 0.038
Identities = 19/75 (25%), Positives = 39/75 (52%), Gaps = 1/75 (1%)
Frame = +2
Query: 521 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML-EPSTKMPWFKGWQVERKE 697
YS+ RF+ IK+ V S+++ + +++ ++P+S N++ PS W
Sbjct: 393 YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLSAMENQNLVAAPSDNR--LSSWY----- 445
Query: 698 GKADGKCLIEALDAI 742
G CL++A+D++
Sbjct: 446 ---QGPCLLDAVDSV 457
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 68.1 bits (159), Expect = 6e-12
Identities = 34/87 (39%), Positives = 52/87 (59%)
Frame = +3
Query: 255 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 434
I+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+ G Q
Sbjct: 130 INTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMP-------Q 182
Query: 435 TREHALLAFTLGVKQLIVGVNKMDSLN 515
T+EH LLA +GV ++V +NK D ++
Sbjct: 183 TKEHILLAKQVGVPDMVVFLNKEDQVD 209
Score = 39.1 bits (87), Expect = 0.003
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +1
Query: 37 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 171
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE 116
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 65.7 bits (153), Expect = 3e-11
Identities = 32/78 (41%), Positives = 48/78 (61%)
Frame = +3
Query: 273 KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL 452
++ET+K + +D PGH D++KNMITG +Q D +L+V+ G QT+EH L
Sbjct: 124 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMP-------QTKEHIL 176
Query: 453 LAFTLGVKQLIVGVNKMD 506
LA +GV L+ +NK+D
Sbjct: 177 LARQVGVPSLVCFLNKVD 194
Score = 32.7 bits (71), Expect = 0.27
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +1
Query: 46 KEKTHINIVVIGHVDSGKSTTT 111
+ K H+N+ IGHVD GK+T T
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLT 84
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 40.7 bits (91), Expect = 0.001
Identities = 23/78 (29%), Positives = 39/78 (50%)
Frame = +3
Query: 282 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 461
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 462 TLGVKQLIVGVNKMDSLN 515
+ +K +I+ NK+D +N
Sbjct: 178 MMRLKHIIILQNKIDLIN 195
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 40.7 bits (91), Expect = 0.001
Identities = 23/78 (29%), Positives = 39/78 (50%)
Frame = +3
Query: 282 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 461
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 462 TLGVKQLIVGVNKMDSLN 515
+ +K +I+ NK+D +N
Sbjct: 178 MMRLKHIIILQNKIDLIN 195
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 38.3 bits (85), Expect = 0.005
Identities = 25/86 (29%), Positives = 41/86 (47%), Gaps = 4/86 (4%)
Frame = +3
Query: 276 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 443
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 444 HALLAFTLGVKQLIVGVNKMDSLNHH 521
H + +K +I+ NK+D + +
Sbjct: 166 HLAAVEIMQLKHIIILQNKIDLIQEN 191
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 37.9 bits (84), Expect = 0.007
Identities = 22/76 (28%), Positives = 37/76 (48%)
Frame = +3
Query: 294 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 473
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 474 KQLIVGVNKMDSLNHH 521
K +I+ NK+D + +
Sbjct: 174 KDIIIIQNKIDLIQEN 189
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 34.3 bits (75), Expect = 0.087
Identities = 28/93 (30%), Positives = 42/93 (45%)
Frame = +3
Query: 279 ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLA 458
E S Y + +ID PGH DF + S A+L+V A G QT + LA
Sbjct: 131 EASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAAQG-------VQAQTVANFYLA 183
Query: 459 FTLGVKQLIVGVNKMDSLNHHTVSPDLRKSRRK 557
F + ++ +NK+D T P+ K++ K
Sbjct: 184 FEANL-TIVPVINKID---QPTADPERVKAQLK 212
Score = 29.1 bits (62), Expect = 3.3
Identities = 29/76 (38%), Positives = 34/76 (44%)
Frame = +1
Query: 28 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKY 207
D K EK N +I H+D GKST L+ G I K G G +Y
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY 102
Query: 208 AWVLDKLKAERERGIT 255
LDKL +RERGIT
Sbjct: 103 ---LDKL--QRERGIT 113
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 33.9 bits (74), Expect = 0.12
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = +3
Query: 255 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 401
+ + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 197 MSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 33.9 bits (74), Expect = 0.12
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = +3
Query: 255 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 401
+ + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 197 MSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 32.7 bits (71), Expect = 0.27
Identities = 38/124 (30%), Positives = 51/124 (41%), Gaps = 1/124 (0%)
Frame = +1
Query: 64 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 243
N +I H+D GKST L+ G + R + K F LD + ERE
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131
Query: 244 RGITSILLSGSSKLASTMLP-SLMLLDTEISSRT*SQEPLRLIALCSS*LPVPVNSKLVS 420
RGIT L + + P L L+DT S E R +A C L V S+ V
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTP-GHVDFSYEVSRSLAACEGALLVVDASQGVE 190
Query: 421 LRTV 432
+T+
Sbjct: 191 AQTL 194
Score = 30.7 bits (66), Expect = 1.1
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +3
Query: 276 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 401
+E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 147 YEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.27
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +3
Query: 297 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 401
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 32.7 bits (71), Expect = 0.27
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +1
Query: 64 NIVVIGHVDSGKSTTTGHLIYKCGG 138
NI ++ HVD GK+T HLI GG
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35
Score = 32.7 bits (71), Expect = 0.27
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +3
Query: 291 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 401
Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 74 YSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 32.7 bits (71), Expect = 0.27
Identities = 12/28 (42%), Positives = 20/28 (71%)
Frame = +1
Query: 52 KTHINIVVIGHVDSGKSTTTGHLIYKCG 135
K + NI ++ H+D+GK+TTT ++Y G
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 31.5 bits (68), Expect = 0.61
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +3
Query: 291 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 446
Y V IID PGH DF + D A+L++ + G I+ + Q R +
Sbjct: 133 YKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVDRQMRRY 184
Score = 31.1 bits (67), Expect = 0.81
Identities = 22/64 (34%), Positives = 34/64 (53%)
Frame = +1
Query: 64 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 243
NI + H+DSGK+T T +++ G R E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTG----RIHEIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 244 RGIT 255
+GIT
Sbjct: 117 KGIT 120
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 31.5 bits (68), Expect = 0.61
Identities = 22/70 (31%), Positives = 35/70 (50%)
Frame = +3
Query: 303 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 482
+ID PGH F G+S D A+L+V + + G+ QT E +L + +
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756
Query: 483 IVGVNKMDSL 512
I+ +NK+D L
Sbjct: 757 IIALNKVDRL 766
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 31.5 bits (68), Expect = 0.61
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +3
Query: 291 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 446
Y V IID PGH DF + D A+L++ + G I+ + Q R +
Sbjct: 133 YKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVDRQMRRY 184
Score = 31.1 bits (67), Expect = 0.81
Identities = 22/64 (34%), Positives = 34/64 (53%)
Frame = +1
Query: 64 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 243
NI + H+DSGK+T T +++ G R E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTG----RIHEIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 244 RGIT 255
+GIT
Sbjct: 117 KGIT 120
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 31.5 bits (68), Expect = 0.61
Identities = 23/72 (31%), Positives = 34/72 (47%)
Frame = +3
Query: 297 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 476
+ +ID PGH F G++ D A+L+V + G+ QT E L VK
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610
Query: 477 QLIVGVNKMDSL 512
I+ +NK+D L
Sbjct: 611 -FIIALNKVDRL 621
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 30.7 bits (66), Expect = 1.1
Identities = 22/70 (31%), Positives = 34/70 (48%)
Frame = +3
Query: 303 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 482
+ID PGH F G+S D A+L+V + G+ QT E +L + +
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DITHGLQP--QTIE-SLNLLRMRNTEF 164
Query: 483 IVGVNKMDSL 512
I+ +NK+D L
Sbjct: 165 IIALNKVDRL 174
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 30.7 bits (66), Expect = 1.1
Identities = 23/70 (32%), Positives = 34/70 (48%)
Frame = +3
Query: 303 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 482
+ID PGH F G+S D A+L+V + G+ QT E +L + +
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIMHGLEP--QTIE-SLNLLRMRNTEF 823
Query: 483 IVGVNKMDSL 512
IV +NK+D L
Sbjct: 824 IVALNKVDRL 833
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.5 bits (63), Expect = 2.5
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +3
Query: 285 SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 386
++Y + +ID+PGH DF + D A+++V
Sbjct: 96 NEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
Score = 29.1 bits (62), Expect = 3.3
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +1
Query: 64 NIVVIGHVDSGKSTTTGHLIYKCG 135
N+ VI HVD GKST T L+ G
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44
>At1g47840.1 68414.m05325 hexokinase, putative similar to hexokinase
1 [Arabidopsis thaliana] Swiss-Prot:Q42525
Length = 493
Score = 28.7 bits (61), Expect = 4.3
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +2
Query: 641 LEPSTKMPWFKGWQVERKEGKADGKCLIEALDA 739
++ T W KG++V EGK CL EA++A
Sbjct: 189 IDSGTLSKWTKGFKVSGMEGKNVVACLNEAMEA 221
>At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containing
protein contains Pfam profile PF01388: ARID/BRIGHT DNA
binding domain
Length = 747
Score = 28.3 bits (60), Expect = 5.7
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = +1
Query: 577 DWLQPSCCRFRAHFWMARRQHVGAFNQNALVQGM 678
DW+ C AHF RR +GAF A G+
Sbjct: 687 DWVNCGSCGEWAHFGCDRRPGLGAFKDYAKTDGL 720
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 5.7
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +2
Query: 524 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 613
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At3g18370.1 68416.m02336 C2 domain-containing protein contains Pfam
profile: PF00168 C2 domain
Length = 815
Score = 28.3 bits (60), Expect = 5.7
Identities = 24/68 (35%), Positives = 28/68 (41%), Gaps = 4/68 (5%)
Frame = +3
Query: 549 RRKYPHTSRRLATTQLLSLS---CPFLDGTETTCWSLQPKCLGSR-DGRWSVKKAKLTEN 716
+R SR + QLL S CP L G TCWS + R D W +
Sbjct: 127 KRLRQRRSRLIENIQLLEFSLGSCPPLLGLHGTCWSKSGEQKIMRLDFNWDTTDLSILLQ 186
Query: 717 ASLKLSMP 740
A KLSMP
Sbjct: 187 A--KLSMP 192
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 28.3 bits (60), Expect = 5.7
Identities = 19/67 (28%), Positives = 29/67 (43%)
Frame = +3
Query: 306 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLI 485
+D PGH F G D A+++VAA G QT E A+ ++
Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG-------IRPQTNE-AIAHAKAAAVPIV 608
Query: 486 VGVNKMD 506
+ +NK+D
Sbjct: 609 IAINKID 615
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 27.9 bits (59), Expect = 7.6
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +2
Query: 530 PRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 640
PRF ++ ++SS ++ Y AAVAF +S W D +
Sbjct: 354 PRFLKLMIQLSSQTNEL-YQLAAVAFCLLSAWCSDKL 389
>At5g06810.1 68418.m00770 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 1141
Score = 27.9 bits (59), Expect = 7.6
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = +1
Query: 115 HLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITSILLS 270
H + G+D + I K KEA+E+ S++ + K+KA + G + + LS
Sbjct: 174 HHVLCYSGVDPKRIGKIFKEAREV--FSYETGVLASKIKAYEDLGFSRLFLS 223
>At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) /
beta-fructofuranosidase / invertase, vacuolar identical
to beta-fructosidase GB:CAA67560 GI:1429209 [Arabidopsis
thaliana]; supported by full-length cDNA GI:14517549;
identical to cDNA Beta-fructosidase GI:3115854
Length = 648
Score = 27.9 bits (59), Expect = 7.6
Identities = 17/54 (31%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Frame = -3
Query: 536 IWAHCMVVQ*IHFVYSYDELFDTEGESEQGMLTG-LTVLRDTSFEFTGTGSYDE 378
+W H + IH++Y + + G+ TG T L D S TGS DE
Sbjct: 148 VWGHAVSKDLIHWLYLPIAMVPDQWYDANGVWTGSATFLDDGSIVMLYTGSTDE 201
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 27.5 bits (58), Expect = 10.0
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +2
Query: 500 NGFTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 619
N TE + P+ E K E+S++I IG+ V F P S
Sbjct: 61 NVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 27.5 bits (58), Expect = 10.0
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +2
Query: 500 NGFTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 619
N TE + P+ E K E+S++I IG+ V F P S
Sbjct: 61 NVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g02010.1 68418.m00120 expressed protein contains Pfam profile
PF03759: Domain of unknown function (DUF315)
Length = 546
Score = 27.5 bits (58), Expect = 10.0
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = -3
Query: 392 GSYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDVIPR 243
G E+S L+ D + + +IN +I LA E+PES ++ +PR
Sbjct: 237 GGLQENSRKQLQHKRDCTNQILKAAMAIN--SITLADMEIPESYLESLPR 284
>At3g05470.1 68416.m00599 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 884
Score = 27.5 bits (58), Expect = 10.0
Identities = 24/85 (28%), Positives = 36/85 (42%)
Frame = -3
Query: 389 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDVIPRSRSAFSLSNTQ 210
S D+ S S+ G + +S + S + V +S E +D+ SRS F +S +
Sbjct: 308 SDDDESFHSVGGGSQYSNPRLSNASSASGSVNVGSSQRFSEHKLDIPECSRSDFGISVSA 367
Query: 209 AYLKDPLPISWASFSNFSMVRLSIP 135
P P FSN + LS P
Sbjct: 368 PPPPPPPPPPLPQFSNKRIHTLSSP 392
>At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha
subunit, chloroplast / 60 kDa chaperonin alpha subunit /
CPN-60 alpha identical to SWISS-PROT:P21238- RuBisCO
subunit binding-protein alpha subunit, chloroplast
precursor (60 kDa chaperonin alpha subunit, CPN-60
alpha) [Arabidopsis thaliana]
Length = 586
Score = 27.5 bits (58), Expect = 10.0
Identities = 16/58 (27%), Positives = 29/58 (50%)
Frame = -3
Query: 389 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDVIPRSRSAFSLSN 216
++D+HS +L+ D + D + ++ N+VL F P+ D + +R A L N
Sbjct: 52 AFDQHSRAALQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKVVNDGVTIAR-AIELPN 108
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,014,099
Number of Sequences: 28952
Number of extensions: 368482
Number of successful extensions: 1197
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 1132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1190
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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