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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00034
         (681 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g06483.1 68416.m00751 pyruvate dehydrogenase (lipoamide) kina...    60   1e-09
At5g57990.1 68418.m07255 ubiquitin-specific protease 23, putativ...    28   5.0  

>At3g06483.1 68416.m00751 pyruvate dehydrogenase (lipoamide) kinase
           (PDHK) nearly identical to pyruvate dehydrogenase kinase
           [Arabidopsis thaliana] GI:3641834
          Length = 369

 Score = 60.1 bits (139), Expect = 1e-09
 Identities = 31/86 (36%), Positives = 53/86 (61%), Gaps = 3/86 (3%)
 Frame = +3

Query: 261 DAYENARFLCDRYYLASPELEVLQDGVSSLRPMPIVYVPSHLYHMLFELFKNAMRAVME- 437
           +A E+AR +C R Y ++PE+ +  D           YVP+HL+ M++EL KN++RAV E 
Sbjct: 199 NASEDARSICFREYGSAPEINIYGDP-----SFTFPYVPTHLHLMMYELVKNSLRAVQER 253

Query: 438 --NHGTAPPPIQVNLVNGKEDISVKI 509
             +     PPI++ + +G ED+++K+
Sbjct: 254 FVDSDRVAPPIRIIVADGIEDVTIKV 279



 Score = 35.9 bits (79), Expect = 0.025
 Identities = 16/26 (61%), Positives = 19/26 (73%)
 Frame = +1

Query: 64  GTENSIQYFLDRFYMSRISIRMLINQ 141
           G  + I  FLDRFY+SRI IRMLI +
Sbjct: 142 GNLDEIHQFLDRFYLSRIGIRMLIGK 167



 Score = 32.7 bits (71), Expect = 0.23
 Identities = 17/35 (48%), Positives = 20/35 (57%), Gaps = 6/35 (17%)
 Frame = +1

Query: 553 FKYMYSTAPQPSKSDSHT------VPLAGYGYGLP 639
           F Y+YSTA  P + D         V +AGYGYGLP
Sbjct: 295 FTYLYSTARNPLEEDVDLGIADVPVTMAGYGYGLP 329



 Score = 29.1 bits (62), Expect = 2.8
 Identities = 13/16 (81%), Positives = 13/16 (81%)
 Frame = +2

Query: 632 GCLISRLYARYFHGDL 679
           G  ISRLYARYF GDL
Sbjct: 327 GLPISRLYARYFGGDL 342


>At5g57990.1 68418.m07255 ubiquitin-specific protease 23, putative
           (UBP23) identical to GI:11993486
          Length = 859

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 18/53 (33%), Positives = 29/53 (54%)
 Frame = +1

Query: 196 IGNGGRHIGSIDPACDVVAVLGTLTRTQGSSATDTT*PHQSWRYCRTECRVCA 354
           IG G +++G+    C + +VL  LT T+  +AT  T  HQ + +    C +CA
Sbjct: 105 IGAGLQNLGN---TCFLNSVLQCLTYTEPLAATLQTAAHQKYCHVAGFCALCA 154


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,724,790
Number of Sequences: 28952
Number of extensions: 338604
Number of successful extensions: 999
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 969
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 997
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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