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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= tesV0256.Seq
         (598 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl...    43   2e-04
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl...    40   0.002
At5g02880.1 68418.m00231 HECT-domain-containing protein / ubiqui...    34   0.063
At4g38600.2 68417.m05463 HECT-domain-containing protein / ubiqui...    29   1.8  
At4g38600.1 68417.m05464 HECT-domain-containing protein / ubiqui...    29   1.8  
At3g53090.1 68416.m05851 HECT-domain-containing protein / ubiqui...    29   2.4  

>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
            identical to ubiquitin-protein ligase 1 [Arabidopsis
            thaliana] GI:7108521; E3, HECT-domain protein family;
            similar to GI:7108521, GB:AAF36454 from [Arabidopsis
            thaliana]
          Length = 3891

 Score = 42.7 bits (96), Expect = 2e-04
 Identities = 36/118 (30%), Positives = 57/118 (48%), Gaps = 10/118 (8%)
 Frame = -1

Query: 487  DFHELEKSTEYDGAYASDSXMLQXFWGIVXQLXXXXXXXXASVHDRVG--PGARGRAVQ- 317
            DF +L+ +TEY  +Y + S ++  FW +V                     P    +A+Q 
Sbjct: 3775 DFDDLKANTEYT-SYTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQG 3833

Query: 316  ------LELCDREXRPGLRPASHRAHFFNVLLLPEYDTRDKLQDRLLKAIN-YSKGFG 164
                  L++      P   P++H    FN L LPEY ++++LQ+RLL AI+  S+GFG
Sbjct: 3834 ISGPQRLQIHKAYGAPERLPSAHTC--FNQLDLPEYQSKEQLQERLLLAIHEASEGFG 3889


>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
            identical to ubiquitin-protein ligase 2 [Arabidopsis
            thaliana] GI:7108523; E3, HECT-domain protein family;
            similar to ubiquitin-protein ligase 2 GI:7108523 from
            [Arabidopsis thaliana]
          Length = 3658

 Score = 39.5 bits (88), Expect = 0.002
 Identities = 34/118 (28%), Positives = 57/118 (48%), Gaps = 10/118 (8%)
 Frame = -1

Query: 487  DFHELEKSTEYDGAYASDSXMLQXFWGIVXQLXXXXXXXXASVHDRVG--PGARGRAVQ- 317
            DF +L+ +TEY  +Y   S +++ FW +V                     P    +A+Q 
Sbjct: 3542 DFDDLKANTEYT-SYTVGSPVIRWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQG 3600

Query: 316  ------LELCDREXRPGLRPASHRAHFFNVLLLPEYDTRDKLQDRLLKAIN-YSKGFG 164
                  L++      P   P++H    FN L LPEY +++++Q+RLL AI+  ++GFG
Sbjct: 3601 ISGPQRLQIHKAYGSPERLPSAHTC--FNQLDLPEYQSKEQVQERLLLAIHEANEGFG 3656


>At5g02880.1 68418.m00231 HECT-domain-containing protein /
            ubiquitin-transferase family protein /
            armadillo/beta-catenin-like repeat-containing protein
            similar to SP|Q14669 Thyroid receptor interacting protein
            12 (TRIP12) {Homo sapiens}; contains Pfam profiles
            PF00632: HECT-domain (ubiquitin-transferase), PF00514:
            Armadillo/beta-catenin-like repeat
          Length = 1502

 Score = 34.3 bits (75), Expect = 0.063
 Identities = 15/30 (50%), Positives = 19/30 (63%)
 Frame = -2

Query: 399  HSLPLEXKRKLLQFTTGSDRVPVGGLCNLN 310
            H    E +R  LQF TGS R+P GGL +L+
Sbjct: 1416 HEFDREQQRAFLQFVTGSPRLPHGGLASLS 1445


>At4g38600.2 68417.m05463 HECT-domain-containing protein /
            ubiquitin-transferase family protein similar to
            SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
            {Homo sapiens}; contains Pfam profile PF00632:
            HECT-domain (ubiquitin-transferase)
          Length = 1794

 Score = 29.5 bits (63), Expect = 1.8
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = -2

Query: 393  LPLEXKRKLLQFTTGSDRVPVGGLCNLN 310
            L  + +R   QF TG+ R+P GGL  LN
Sbjct: 1698 LTADQQRAFCQFVTGAPRLPPGGLAVLN 1725



 Score = 27.1 bits (57), Expect = 9.5
 Identities = 12/27 (44%), Positives = 17/27 (62%)
 Frame = -1

Query: 250  NVLLLPEYDTRDKLQDRLLKAINYSKG 170
            N L LP Y T++ +  +LL AIN  +G
Sbjct: 1763 NYLKLPPYSTKEIMYKKLLYAINEGQG 1789


>At4g38600.1 68417.m05464 HECT-domain-containing protein /
            ubiquitin-transferase family protein similar to
            SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
            {Homo sapiens}; contains Pfam profile PF00632:
            HECT-domain (ubiquitin-transferase)
          Length = 1888

 Score = 29.5 bits (63), Expect = 1.8
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = -2

Query: 393  LPLEXKRKLLQFTTGSDRVPVGGLCNLN 310
            L  + +R   QF TG+ R+P GGL  LN
Sbjct: 1792 LTADQQRAFCQFVTGAPRLPPGGLAVLN 1819



 Score = 27.1 bits (57), Expect = 9.5
 Identities = 12/27 (44%), Positives = 17/27 (62%)
 Frame = -1

Query: 250  NVLLLPEYDTRDKLQDRLLKAINYSKG 170
            N L LP Y T++ +  +LL AIN  +G
Sbjct: 1857 NYLKLPPYSTKEIMYKKLLYAINEGQG 1883


>At3g53090.1 68416.m05851 HECT-domain-containing protein /
            ubiquitin-transferase family protein / IQ
            calmodulin-binding motif-containing protein contains Pfam
            profiles PF00632: HECT-domain (ubiquitin-transferase),
            PF00612: IQ calmodulin-binding motif
          Length = 1142

 Score = 29.1 bits (62), Expect = 2.4
 Identities = 9/29 (31%), Positives = 18/29 (62%)
 Frame = -1

Query: 487  DFHELEKSTEYDGAYASDSXMLQXFWGIV 401
            D  +L ++T+Y G Y+  S  ++ FW ++
Sbjct: 1020 DVDDLRRNTKYTGGYSDSSRTIKIFWEVM 1048


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,594,598
Number of Sequences: 28952
Number of extensions: 176209
Number of successful extensions: 420
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 411
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 420
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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