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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0099.Seq
         (479 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) simi...   123   7e-29
At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB)           116   8e-27
At3g52640.1 68416.m05799 nicastrin-related contains weak similar...    30   0.70 
At5g45490.1 68418.m05588 disease resistance protein-related cont...    29   1.6  
At1g12710.1 68414.m01474 F-box family protein / SKP1 interacting...    27   5.0  
At3g52640.2 68416.m05800 nicastrin-related contains weak similar...    27   8.7  

>At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) similar
           to 40S ribosomal protein S3A (S phase specific protein
           GBIS289) GB:P49396 [Brassica rapa]
          Length = 262

 Score =  123 bits (296), Expect = 7e-29
 Identities = 61/83 (73%), Positives = 68/83 (81%)
 Frame = +2

Query: 2   FTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDAERSF 181
           F++KDWYDVKAPS+F+ R VG TLV+RTQGTKIASEGLK RVFEVSLADLQ D D   ++
Sbjct: 24  FSKKDWYDVKAPSIFTHRNVGKTLVSRTQGTKIASEGLKHRVFEVSLADLQGDED--NAY 81

Query: 182 RKFRLIAEYVQGRNVLCNXHGMD 250
           RK RL AE VQGRNVLC   GMD
Sbjct: 82  RKIRLRAEDVQGRNVLCQFWGMD 104



 Score = 95.9 bits (228), Expect = 1e-20
 Identities = 41/74 (55%), Positives = 58/74 (78%)
 Frame = +1

Query: 256 TDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGXTNKDSLSQRKTCYAQHTQVRAIRK 435
           TDKLR +VKKWQTLIEA++DVKTTD Y LR+FCI  T + +   ++TCYAQ +Q+R IR+
Sbjct: 107 TDKLRSLVKKWQTLIEAHVDVKTTDSYTLRLFCIAFTKRRANQVKRTCYAQSSQIRQIRR 166

Query: 436 KMCEIITRDVTNSE 477
           KM +I+ R+ ++ +
Sbjct: 167 KMRDIMVREASSCD 180


>At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB)
          Length = 262

 Score =  116 bits (279), Expect = 8e-27
 Identities = 59/83 (71%), Positives = 65/83 (78%)
 Frame = +2

Query: 2   FTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDAERSF 181
           F++KDWYDVKAP  F+ R VG TLV+RTQGTKIASEGLK RVFEVSLADLQ D D   ++
Sbjct: 24  FSKKDWYDVKAPGSFTNRNVGKTLVSRTQGTKIASEGLKHRVFEVSLADLQNDED--NAY 81

Query: 182 RKFRLIAEYVQGRNVLCNXHGMD 250
           RK RL AE VQGRNVL    GMD
Sbjct: 82  RKIRLRAEDVQGRNVLTQFWGMD 104



 Score = 99.1 bits (236), Expect = 1e-21
 Identities = 42/74 (56%), Positives = 59/74 (79%)
 Frame = +1

Query: 256 TDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGXTNKDSLSQRKTCYAQHTQVRAIRK 435
           TDKLR +VKKWQTLIEA++DVKTTDGY LR+FCI  T + +   ++TCYAQ +Q+R IR+
Sbjct: 107 TDKLRSLVKKWQTLIEAHVDVKTTDGYTLRMFCIAFTKRRANQVKRTCYAQSSQIRQIRR 166

Query: 436 KMCEIITRDVTNSE 477
           KM EI+ ++ ++ +
Sbjct: 167 KMSEIMVKEASSCD 180


>At3g52640.1 68416.m05799 nicastrin-related contains weak similarity
           to Nicastrin precursor (Swiss-Prot:Q92542) [Homo
           sapiens]
          Length = 676

 Score = 30.3 bits (65), Expect = 0.70
 Identities = 19/58 (32%), Positives = 32/58 (55%), Gaps = 3/58 (5%)
 Frame = -3

Query: 453 NDFTH-FLSDCSDLSVLGVARLA--LAQGVLIGXTNAEDT*NVSIGCLHINVGFDESL 289
           N F H  L D S+++   V   A  +A+ + I  ++ +DT N ++G +H+N  F E L
Sbjct: 430 NKFYHSHLDDLSNINSSSVVAAASVVARTLYILASDNKDTSNSALGSIHVNASFVEEL 487


>At5g45490.1 68418.m05588 disease resistance protein-related
           contains Pfam domain, PF00931: NB-ARC domain, a novel
           signalling motif found in plant resistance gene products
          Length = 354

 Score = 29.1 bits (62), Expect = 1.6
 Identities = 22/80 (27%), Positives = 33/80 (41%), Gaps = 4/80 (5%)
 Frame = +2

Query: 5   TRKDWYDVK--APSMFSKRQVGTTLVNRTQG-TKIASEGLKGRVFEV-SLADLQADTDAE 172
           T KDW DV   A     K++       R +  +K+    + G   E+ SL     D    
Sbjct: 22  TCKDWLDVNGLAKGNLEKKRDDNEEEERLKTESKLPGHDIHGFDNEIKSLQHFLLDQKVR 81

Query: 173 RSFRKFRLIAEYVQGRNVLC 232
           R F+   ++ EY  G+  LC
Sbjct: 82  REFKSLVIVGEYGVGKTALC 101


>At1g12710.1 68414.m01474 F-box family protein / SKP1 interacting
           partner 3-related contains Pfam profile PF00646: F-box
           domain
          Length = 291

 Score = 27.5 bits (58), Expect = 5.0
 Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 3/37 (8%)
 Frame = +2

Query: 215 GRNVLCNX---HGMDLKPISSGGWLKNGRLSSKPTLM 316
           GR V CN    HG D+KP+    W ++G+ SS   ++
Sbjct: 195 GRRV-CNTEQVHGWDIKPVRFQLWTEDGQYSSSQCML 230


>At3g52640.2 68416.m05800 nicastrin-related contains weak similarity
           to Nicastrin precursor (Swiss-Prot:Q92542) [Homo
           sapiens]
          Length = 705

 Score = 26.6 bits (56), Expect = 8.7
 Identities = 14/42 (33%), Positives = 26/42 (61%)
 Frame = -3

Query: 414 SVLGVARLALAQGVLIGXTNAEDT*NVSIGCLHINVGFDESL 289
           SV+  A + +A+ + I  ++ +DT N ++G +H+N  F E L
Sbjct: 476 SVVAAASV-VARTLYILASDNKDTSNSALGSIHVNASFVEEL 516


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,073,389
Number of Sequences: 28952
Number of extensions: 194661
Number of successful extensions: 541
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 529
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 539
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 819227264
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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