SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0048
         (727 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g17380.1 68418.m02038 pyruvate decarboxylase family protein s...    91   5e-19
At1g54300.1 68414.m06190 hypothetical protein                          33   0.19 
At3g48560.1 68416.m05302 acetolactate synthase, chloroplast / ac...    32   0.45 
At5g54960.1 68418.m06845 pyruvate decarboxylase, putative strong...    30   1.8  
At5g01320.1 68418.m00044 pyruvate decarboxylase, putative strong...    30   1.8  
At5g01330.1 68418.m00045 pyruvate decarboxylase, putative strong...    29   2.4  
At4g33070.1 68417.m04711 pyruvate decarboxylase, putative strong...    29   2.4  
At3g07870.1 68416.m00962 F-box family protein contains F-box dom...    29   3.1  
At2g35110.1 68415.m04307 HEM protein-related weak similarity to ...    28   7.2  
At2g17440.1 68415.m02012 leucine-rich repeat family protein cont...    27   9.6  

>At5g17380.1 68418.m02038 pyruvate decarboxylase family protein
           similar to 2-hydroxyphytanoyl-CoA lyase [Homo sapiens]
           GI:6273457; contains InterPro entry IPR000399: Pyruvate
           decarboxylase
          Length = 572

 Score = 91.5 bits (217), Expect = 5e-19
 Identities = 40/78 (51%), Positives = 56/78 (71%)
 Frame = +3

Query: 9   ENTKLPFLPTPMGKGVVADESEYCVSTARTQALLKADVILLLGARMNWMLHFGQPPRYAP 188
           E T +PFLPTPMGKG++ D  E+  + AR+ A+ K DV L++GAR+NW+LHFG+ P++  
Sbjct: 241 EITGIPFLPTPMGKGLLPDTHEFSATAARSLAIGKCDVALVVGARLNWLLHFGESPKWDK 300

Query: 189 DVKIIQVEISPEEFHNSK 242
           DVK I V++S EE    K
Sbjct: 301 DVKFILVDVSEEEIELRK 318



 Score = 85.0 bits (201), Expect = 5e-17
 Identities = 41/72 (56%), Positives = 52/72 (72%)
 Frame = +3

Query: 510 GRGLLLNNHPRHRLDAGTFGTMGVGPGFAIAAAMWCRDYAPGKRVICVEGDSAFGFSGME 689
           GR +L+   PR RLDAGT+GTMGVG G+ IAAA+     +P + V+ VEGDS FGFS ME
Sbjct: 411 GRSVLVQKEPRTRLDAGTWGTMGVGLGYCIAAAV----ASPDRLVVAVEGDSGFGFSAME 466

Query: 690 IETMFRYKLPVI 725
           +ET+ RY L V+
Sbjct: 467 VETLVRYNLAVV 478



 Score = 37.1 bits (82), Expect = 0.012
 Identities = 20/59 (33%), Positives = 30/59 (50%), Gaps = 4/59 (6%)
 Frame = +1

Query: 346 VAGTETEQKANTEFVEAQASSTAVPLNYYTVFKTVQQGI----PKDSIIVSEGANTMDM 510
           V     + K N E +E Q +   VP N+ T  + ++  I        ++VSEGANTMD+
Sbjct: 352 VESISKKAKENGEKMEIQLAKDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEGANTMDV 410


>At1g54300.1 68414.m06190 hypothetical protein
          Length = 314

 Score = 33.1 bits (72), Expect = 0.19
 Identities = 26/74 (35%), Positives = 39/74 (52%), Gaps = 2/74 (2%)
 Frame = +3

Query: 474 NHCQRRSEYHGYGRGLLLNNHPRHRLDAGTFGTMGVGPG-FAIAAAMWCRD-YAPGKRVI 647
           N+  R S  H +  G  +NN P+H    G  G +GVG G F++A   W R+ Y+P   V 
Sbjct: 163 NNSSRNS--HSHTVGFNVNNGPQHHFSQG-IGGVGVGEGNFSMANGGWQRNSYSP---VN 216

Query: 648 CVEGDSAFGFSGME 689
            +  ++A  F GM+
Sbjct: 217 SLNYNTANEFFGMQ 230


>At3g48560.1 68416.m05302 acetolactate synthase, chloroplast /
           acetohydroxy-acid synthase (ALS) nearly identical to
           SP|P17597 Acetolactate synthase, chloroplast precursor
           (EC 2.2.1.6, formerly EC 4.1.3.18) (Acetohydroxy-acid
           synthase) (ALS) {Arabidopsis thaliana}
          Length = 670

 Score = 31.9 bits (69), Expect = 0.45
 Identities = 21/62 (33%), Positives = 31/62 (50%)
 Frame = +3

Query: 537 PRHRLDAGTFGTMGVGPGFAIAAAMWCRDYAPGKRVICVEGDSAFGFSGMEIETMFRYKL 716
           PR  L +G  G MG G   AI A++      P   V+ ++GD +F  +  E+ T+    L
Sbjct: 501 PRQWLSSGGLGAMGFGLPAAIGASV----ANPDAIVVDIDGDGSFIMNVQELATIRVENL 556

Query: 717 PV 722
           PV
Sbjct: 557 PV 558


>At5g54960.1 68418.m06845 pyruvate decarboxylase, putative strong
           similarity to pyruvate decarboxylase 1 [Vitis vinifera]
           GI:10732644; contains InterPro entry IPR000399: Pyruvate
           decarboxylase
          Length = 607

 Score = 29.9 bits (64), Expect = 1.8
 Identities = 12/40 (30%), Positives = 21/40 (52%)
 Frame = +3

Query: 588 GFAIAAAMWCRDYAPGKRVICVEGDSAFGFSGMEIETMFR 707
           G+++ A +      P +RVI   GD +F  +  ++ TM R
Sbjct: 460 GWSVGATLGYAQAMPNRRVIACIGDGSFQVTAQDVSTMIR 499


>At5g01320.1 68418.m00044 pyruvate decarboxylase, putative strong
           similarity to pyruvate decarboxylase 1 [Vitis vinifera]
           GI:10732644; contains InterPro entry IPR000399: Pyruvate
           decarboxylase
          Length = 603

 Score = 29.9 bits (64), Expect = 1.8
 Identities = 13/40 (32%), Positives = 21/40 (52%)
 Frame = +3

Query: 588 GFAIAAAMWCRDYAPGKRVICVEGDSAFGFSGMEIETMFR 707
           G+++ A +      P KRV+   GD +F  +  +I TM R
Sbjct: 456 GWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDISTMIR 495


>At5g01330.1 68418.m00045 pyruvate decarboxylase, putative strong
           similarity to pyruvate decarboxylase 1 [Vitis vinifera]
           GI:10732644; contains InterPro entry IPR000399: Pyruvate
           decarboxylase
          Length = 592

 Score = 29.5 bits (63), Expect = 2.4
 Identities = 12/40 (30%), Positives = 21/40 (52%)
 Frame = +3

Query: 588 GFAIAAAMWCRDYAPGKRVICVEGDSAFGFSGMEIETMFR 707
           G+++ A +      P KRV+   GD +F  +  ++ TM R
Sbjct: 445 GWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDVSTMIR 484


>At4g33070.1 68417.m04711 pyruvate decarboxylase, putative strong
           similarity to SP|P51846 Pyruvate decarboxylase isozyme 2
           (EC 4.1.1.1) (PDC) {Nicotiana tabacum}; contains
           InterPro entry IPR000399: Pyruvate decarboxylase
          Length = 607

 Score = 29.5 bits (63), Expect = 2.4
 Identities = 13/40 (32%), Positives = 22/40 (55%)
 Frame = +3

Query: 588 GFAIAAAMWCRDYAPGKRVICVEGDSAFGFSGMEIETMFR 707
           G+++ A +     +P KRV+   GD +F  +  +I TM R
Sbjct: 460 GWSVGATLGYAQASPEKRVLAFIGDGSFQVTVQDISTMLR 499


>At3g07870.1 68416.m00962 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 417

 Score = 29.1 bits (62), Expect = 3.1
 Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
 Frame = +3

Query: 138 ARMNWMLHF-GQPPRYAPDVKIIQVEISPEEFHNSKKSE 251
           A +N  LHF  +P R+ PD K +  ++  EEF    K +
Sbjct: 241 ALVNGRLHFVTRPRRHVPDRKFVSFDLEDEEFKEIPKPD 279


>At2g35110.1 68415.m04307 HEM protein-related weak similarity to
            Membrane-associated protein Hem (Dhem-2)
            (Swiss-Prot:P55162) [Drosophila melanogaster]; weak
            similarity to Nck-associated protein 1 (NAP 1) (p125Nap1)
            (Membrane-associated protein HEM-2) (Swiss-Prot:P55161)
            [Rattus norvegicus]
          Length = 1339

 Score = 27.9 bits (59), Expect = 7.2
 Identities = 15/38 (39%), Positives = 22/38 (57%)
 Frame = -1

Query: 160  CSIQFILAPRSSMTSAFSRACVRAVDTQYSDSSATTPL 47
            C I   L PRSS+ S      +R++ TQY  ++ +TPL
Sbjct: 1179 CEISPYL-PRSSLESHVPYTILRSIYTQYYSNTPSTPL 1215


>At2g17440.1 68415.m02012 leucine-rich repeat family protein
           contains Pfam PF00560: Leucine Rich Repeats
          Length = 526

 Score = 27.5 bits (58), Expect = 9.6
 Identities = 14/55 (25%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
 Frame = +1

Query: 304 VRKEVLIATSKQQLVAGTETEQKANTEFVE--AQASSTAVPLNYYTVFKTVQQGI 462
           +R E+    +   L+   E E +   E ++   + SS+ VPL  + VFK +++ +
Sbjct: 20  LRPEIDDVETATSLIQNVEKEDRNRLEAIDKLVKTSSSEVPLELFNVFKEMKKSL 74


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,116,031
Number of Sequences: 28952
Number of extensions: 336220
Number of successful extensions: 900
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 875
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 899
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -