Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30064
(604 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53580.2 68414.m06085 hydroxyacylglutathione hydrolase, putat... 182 1e-46
At1g53580.1 68414.m06084 hydroxyacylglutathione hydrolase, putat... 182 1e-46
At3g10850.1 68416.m01307 hydroxyacylglutathione hydrolase, cytop... 63 2e-10
At1g06130.2 68414.m00643 hydroxyacylglutathione hydrolase, putat... 62 3e-10
At1g06130.1 68414.m00642 hydroxyacylglutathione hydrolase, putat... 62 3e-10
At2g31350.2 68415.m03830 hydroxyacylglutathione hydrolase, putat... 61 6e-10
At2g31350.1 68415.m03829 hydroxyacylglutathione hydrolase, putat... 61 6e-10
At2g43430.2 68415.m05397 hydroxyacylglutathione hydrolase, mitoc... 55 3e-08
At2g43430.1 68415.m05398 hydroxyacylglutathione hydrolase, mitoc... 55 3e-08
At2g02880.1 68415.m00238 mucin-related similar to putative mucin... 29 3.1
At1g25375.1 68414.m03149 metallo-beta-lactamase family protein 29 3.1
At3g02610.1 68416.m00252 acyl-[acyl-carrier-protein] desaturase,... 28 4.1
At4g07825.1 68417.m01238 expressed protein this may be a pseudog... 27 7.2
At5g30341.1 68418.m03656 hypothetical protein 27 9.6
At3g08850.1 68416.m01029 transducin family protein / WD-40 repea... 27 9.6
>At1g53580.2 68414.m06085 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
GI:1644427 from (Arabidopsis thaliana)
Length = 256
Score = 182 bits (444), Expect = 1e-46
Identities = 89/158 (56%), Positives = 113/158 (71%), Gaps = 6/158 (3%)
Frame = +2
Query: 134 GKLKSLVPGTKSVIGKDSGAQADIHLLDGDLVRFGSHELLAAATPGHTNGCLTYICHEQS 313
G LK+ +PG KSVI K SG++AD+ L GD V G L ATPGHT GC+TY+ E +
Sbjct: 82 GLLKTKLPGVKSVISKASGSKADLFLEPGDKVSIGDIYLEVRATPGHTAGCVTYVTGEGA 141
Query: 314 ------LAFTGDTLLIRGCGRTDFQEGNSETLYKSVHNRIFTLPDEYVLYPAHDYRGQTA 475
+AFTGD +LIRGCGRTDFQEG+S+ LY+SVH++IFTLP + ++YPAHDY+G
Sbjct: 142 DQPQPRMAFTGDAVLIRGCGRTDFQEGSSDQLYESVHSQIFTLPKDTLIYPAHDYKGFEV 201
Query: 476 TSVAEEKKYNPRLTKSLAEFVEIMDNLNLPYPKMINKA 589
++V EE ++NPRLTK F IM NLNL YPKMI+ A
Sbjct: 202 STVGEEMQHNPRLTKDKETFKTIMSNLNLSYPKMIDVA 239
>At1g53580.1 68414.m06084 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
GI:1644427 from (Arabidopsis thaliana)
Length = 256
Score = 182 bits (444), Expect = 1e-46
Identities = 89/158 (56%), Positives = 113/158 (71%), Gaps = 6/158 (3%)
Frame = +2
Query: 134 GKLKSLVPGTKSVIGKDSGAQADIHLLDGDLVRFGSHELLAAATPGHTNGCLTYICHEQS 313
G LK+ +PG KSVI K SG++AD+ L GD V G L ATPGHT GC+TY+ E +
Sbjct: 82 GLLKTKLPGVKSVISKASGSKADLFLEPGDKVSIGDIYLEVRATPGHTAGCVTYVTGEGA 141
Query: 314 ------LAFTGDTLLIRGCGRTDFQEGNSETLYKSVHNRIFTLPDEYVLYPAHDYRGQTA 475
+AFTGD +LIRGCGRTDFQEG+S+ LY+SVH++IFTLP + ++YPAHDY+G
Sbjct: 142 DQPQPRMAFTGDAVLIRGCGRTDFQEGSSDQLYESVHSQIFTLPKDTLIYPAHDYKGFEV 201
Query: 476 TSVAEEKKYNPRLTKSLAEFVEIMDNLNLPYPKMINKA 589
++V EE ++NPRLTK F IM NLNL YPKMI+ A
Sbjct: 202 STVGEEMQHNPRLTKDKETFKTIMSNLNLSYPKMIDVA 239
>At3g10850.1 68416.m01307 hydroxyacylglutathione hydrolase,
cytoplasmic / glyoxalase II (GLX2-2) identical to
SP|O24496 Hydroxyacylglutathione hydrolase cytoplasmic
(EC 3.1.2.6) (Glyoxalase II) (Glx II) {Arabidopsis
thaliana}
Length = 258
Score = 62.9 bits (146), Expect = 2e-10
Identities = 43/137 (31%), Positives = 65/137 (47%), Gaps = 6/137 (4%)
Frame = +2
Query: 137 KLKSLVPGTKSVIGKDSGAQADIHLLD-GDLVRFGSH-ELLAAATPGHTNGCLTYICH-- 304
K+K LVP K G + +D GD + G +LA TP HT G ++Y +
Sbjct: 65 KIKQLVPDIKVYGGSLDKVKGCTDAVDNGDKLTLGQDINILALHTPCHTKGHISYYVNGK 124
Query: 305 --EQSLAFTGDTLLIRGCGRTDFQEGNSETLYKSVHNRIFTLPDEYVLYPAHDYRGQTAT 478
E FTGDTL + GCG+ F EG +E +Y+S+ + LP +Y H+Y +
Sbjct: 125 EGENPAVFTGDTLFVAGCGK--FFEGTAEQMYQSLCVTLAALPKPTQVYCGHEYTVKNLE 182
Query: 479 SVAEEKKYNPRLTKSLA 529
+ N ++ + LA
Sbjct: 183 FALTVEPNNGKIQQKLA 199
>At1g06130.2 68414.m00643 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme GB:AAC49865 GI:2570338 from [Arabidopsis
thaliana]
Length = 330
Score = 62.1 bits (144), Expect = 3e-10
Identities = 40/128 (31%), Positives = 55/128 (42%)
Frame = +2
Query: 167 SVIGKDSGAQADIHLLDGDLVRFGSHELLAAATPGHTNGCLTYICHEQSLAFTGDTLLIR 346
S +D D+ L D D F HE+ TPGHT G +++ FTGDTL
Sbjct: 151 SAADRDRIPGIDVALKDADKWMFAGHEVHIMETPGHTRGHISFYFPGARAIFTGDTLFSL 210
Query: 347 GCGRTDFQEGNSETLYKSVHNRIFTLPDEYVLYPAHDYRGQTATSVAEEKKYNPRLTKSL 526
CG+ EG E + S+ RI LPD+ +Y H+Y + + N L
Sbjct: 211 SCGK--LFEGTPEQMLASL-QRIIALPDDTSVYCGHEYTLSNSKFALSIEPTNEVLQSYA 267
Query: 527 AEFVEIMD 550
A E+ D
Sbjct: 268 AYVAELRD 275
>At1g06130.1 68414.m00642 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme GB:AAC49865 GI:2570338 from [Arabidopsis
thaliana]
Length = 331
Score = 62.1 bits (144), Expect = 3e-10
Identities = 40/128 (31%), Positives = 55/128 (42%)
Frame = +2
Query: 167 SVIGKDSGAQADIHLLDGDLVRFGSHELLAAATPGHTNGCLTYICHEQSLAFTGDTLLIR 346
S +D D+ L D D F HE+ TPGHT G +++ FTGDTL
Sbjct: 152 SAADRDRIPGIDVALKDADKWMFAGHEVHIMETPGHTRGHISFYFPGARAIFTGDTLFSL 211
Query: 347 GCGRTDFQEGNSETLYKSVHNRIFTLPDEYVLYPAHDYRGQTATSVAEEKKYNPRLTKSL 526
CG+ EG E + S+ RI LPD+ +Y H+Y + + N L
Sbjct: 212 SCGK--LFEGTPEQMLASL-QRIIALPDDTSVYCGHEYTLSNSKFALSIEPTNEVLQSYA 268
Query: 527 AEFVEIMD 550
A E+ D
Sbjct: 269 AYVAELRD 276
>At2g31350.2 68415.m03830 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme [Arabidopsis thaliana] gi|2570338|gb|AAC49865
Length = 323
Score = 60.9 bits (141), Expect = 6e-10
Identities = 41/133 (30%), Positives = 63/133 (47%)
Frame = +2
Query: 167 SVIGKDSGAQADIHLLDGDLVRFGSHELLAAATPGHTNGCLTYICHEQSLAFTGDTLLIR 346
S + KD D+ L DGD F HE+ TPGHT G ++ FTGDT+
Sbjct: 146 SAMDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGHTKGHISLYFPGSRAIFTGDTMFSL 205
Query: 347 GCGRTDFQEGNSETLYKSVHNRIFTLPDEYVLYPAHDYRGQTATSVAEEKKYNPRLTKSL 526
CG+ EG + + S+ +I +LPD+ +Y H+Y + + A + N + +S
Sbjct: 206 SCGK--LFEGTPKQMLASL-QKITSLPDDTSIYCGHEYT-LSNSKFALSLEPNNEVLQSY 261
Query: 527 AEFVEIMDNLNLP 565
A V + + LP
Sbjct: 262 AAHVAELRSKKLP 274
>At2g31350.1 68415.m03829 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme [Arabidopsis thaliana] gi|2570338|gb|AAC49865
Length = 324
Score = 60.9 bits (141), Expect = 6e-10
Identities = 41/133 (30%), Positives = 63/133 (47%)
Frame = +2
Query: 167 SVIGKDSGAQADIHLLDGDLVRFGSHELLAAATPGHTNGCLTYICHEQSLAFTGDTLLIR 346
S + KD D+ L DGD F HE+ TPGHT G ++ FTGDT+
Sbjct: 147 SAMDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGHTKGHISLYFPGSRAIFTGDTMFSL 206
Query: 347 GCGRTDFQEGNSETLYKSVHNRIFTLPDEYVLYPAHDYRGQTATSVAEEKKYNPRLTKSL 526
CG+ EG + + S+ +I +LPD+ +Y H+Y + + A + N + +S
Sbjct: 207 SCGK--LFEGTPKQMLASL-QKITSLPDDTSIYCGHEYT-LSNSKFALSLEPNNEVLQSY 262
Query: 527 AEFVEIMDNLNLP 565
A V + + LP
Sbjct: 263 AAHVAELRSKKLP 275
>At2g43430.2 68415.m05397 hydroxyacylglutathione hydrolase,
mitochondrial / glyoxalase II (GLX2-1) identical to
SP|O24495 Hydroxyacylglutathione hydrolase,
mitochondrial precursor (EC 3.1.2.6) (Glyoxalase II)
(Glx II) {Arabidopsis thaliana}
Length = 313
Score = 55.2 bits (127), Expect = 3e-08
Identities = 32/93 (34%), Positives = 45/93 (48%)
Frame = +2
Query: 167 SVIGKDSGAQADIHLLDGDLVRFGSHELLAAATPGHTNGCLTYICHEQSLAFTGDTLLIR 346
S + KD DI L D D F HE+ TPGHT G +++ + FTGD +
Sbjct: 136 SAVDKDRIPGIDILLKDSDKWMFAGHEVRILDTPGHTQGHISFYFPGSATIFTGDLIYSL 195
Query: 347 GCGRTDFQEGNSETLYKSVHNRIFTLPDEYVLY 445
CG EG E + S+ +I +LPD+ +Y
Sbjct: 196 SCG--TLSEGTPEQMLSSL-QKIVSLPDDTNIY 225
>At2g43430.1 68415.m05398 hydroxyacylglutathione hydrolase,
mitochondrial / glyoxalase II (GLX2-1) identical to
SP|O24495 Hydroxyacylglutathione hydrolase,
mitochondrial precursor (EC 3.1.2.6) (Glyoxalase II)
(Glx II) {Arabidopsis thaliana}
Length = 331
Score = 55.2 bits (127), Expect = 3e-08
Identities = 32/93 (34%), Positives = 45/93 (48%)
Frame = +2
Query: 167 SVIGKDSGAQADIHLLDGDLVRFGSHELLAAATPGHTNGCLTYICHEQSLAFTGDTLLIR 346
S + KD DI L D D F HE+ TPGHT G +++ + FTGD +
Sbjct: 154 SAVDKDRIPGIDILLKDSDKWMFAGHEVRILDTPGHTQGHISFYFPGSATIFTGDLIYSL 213
Query: 347 GCGRTDFQEGNSETLYKSVHNRIFTLPDEYVLY 445
CG EG E + S+ +I +LPD+ +Y
Sbjct: 214 SCG--TLSEGTPEQMLSSL-QKIVSLPDDTNIY 243
>At2g02880.1 68415.m00238 mucin-related similar to putative mucin
GI:18071389 [Oryza sativa]
Length = 314
Score = 28.7 bits (61), Expect = 3.1
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = -2
Query: 207 WISAWAPESLPMTLLVPGTSDFNFP 133
W SAW P+ L + P D NFP
Sbjct: 50 WESAWLPDDLTDKIRAPWERDVNFP 74
>At1g25375.1 68414.m03149 metallo-beta-lactamase family protein
Length = 524
Score = 28.7 bits (61), Expect = 3.1
Identities = 24/99 (24%), Positives = 42/99 (42%), Gaps = 1/99 (1%)
Frame = +2
Query: 161 TKSVIGKDSGAQADIHLLDGDLVRFGSHELLAAATPGHTNGCLTYICHEQSLAFTGDTLL 340
T+ IG SG + G+ + +L PGHT+G ++ + GD +
Sbjct: 294 TRHRIGGWSGNYTPVS--GGENIYVNGQKLTVIFAPGHTDGHMSLLHTSTQSLIVGDHCV 351
Query: 341 IRGCGRTDFQEGNSETLY-KSVHNRIFTLPDEYVLYPAH 454
+G D + G + T Y +S + + P+ V+ P H
Sbjct: 352 GQGSAFLDIRAGGNMTDYFQSTYKFLELSPN--VVIPMH 388
>At3g02610.1 68416.m00252 acyl-[acyl-carrier-protein] desaturase,
putative / stearoyl-ACP desaturase, putative similar to
Acyl-[acyl-carrier protein] desaturase from Spinacia
oleracea SP|P28645, Cucumis sativus SP|P32061, Ricinus
communis SP|P22337; contains Pfam profile PF03405 Fatty
acid desaturase
Length = 411
Score = 28.3 bits (60), Expect = 4.1
Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 4/51 (7%)
Frame = +2
Query: 290 TYICHEQS--LAFT-GDTLLIRGCGRTDFQEGNSETLYKSVHNRIFTL-PD 430
T+I H + LA T GDT L + CG E ET Y + ++F + PD
Sbjct: 242 TFISHGNTAKLATTYGDTTLAKICGTIAADEKRHETAYTRIVEKLFEIDPD 292
>At4g07825.1 68417.m01238 expressed protein this may be a
pseudogene. A long ORF is frameshifted.
Length = 247
Score = 27.5 bits (58), Expect = 7.2
Identities = 23/89 (25%), Positives = 38/89 (42%), Gaps = 2/89 (2%)
Frame = +3
Query: 249 SSLLRLRA--TLMDA*LTYVTNSHWRSLVTPS*SGVAAAPTSRRVTLRPSTNRSTIEYSL 422
SSL+ RA TL + +L P S +A+ P + S +T+ +SL
Sbjct: 158 SSLMEWRADLTLKPVMMILTAEVLILNLTKPCCSFIASYPYTCGGINNISPTEATLGFSL 217
Query: 423 CPTSTCCIPRTITEGRLRHLSPKRRNTIP 509
S CC P +++ +LS ++ P
Sbjct: 218 LGLSNCCSPSSLSRPTSTNLSLSHNHSSP 246
>At5g30341.1 68418.m03656 hypothetical protein
Length = 153
Score = 27.1 bits (57), Expect = 9.6
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -2
Query: 225 RSPSSRWISAWAPESLPMTLLVPGTSD 145
+S S+W S WA L M LL SD
Sbjct: 6 KSVGSKWASLWASNGLRMGLLFSSLSD 32
>At3g08850.1 68416.m01029 transducin family protein / WD-40 repeat
family protein similar to WD-repeat protein mip1
(SP:P87141) [Schizosaccharomyces pombe]; contains Pfam
PF00400: WD domain, G-beta repeat (5 copies, 1 weak)
Length = 1344
Score = 27.1 bits (57), Expect = 9.6
Identities = 15/60 (25%), Positives = 27/60 (45%)
Frame = -2
Query: 282 PLVWPGVAAARSSWLPNRTRSPSSRWISAWAPESLPMTLLVPGTSDFNFPSAGYMVGMHV 103
PLV VA A + + + S W P+S + +P + F+ P + +V +H+
Sbjct: 706 PLVRAEVAVALARFAFGHKQHLKLAAASYWKPQSSSLLTSLPSIAKFHDPGSATIVSLHM 765
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,572,866
Number of Sequences: 28952
Number of extensions: 286238
Number of successful extensions: 891
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 848
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 884
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1197101088
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -