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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS30060
         (814 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g45540.1 68415.m05663 WD-40 repeat family protein / beige-rel...    32   0.52 
At5g42370.1 68418.m05159 expressed protein                             30   2.1  
At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to ...    29   4.9  
At5g19700.1 68418.m02343 MATE efflux protein-related contains Pf...    29   4.9  
At2g19150.1 68415.m02235 pectinesterase family protein contains ...    29   4.9  
At3g29033.1 68416.m03629 glycine-rich protein                          28   6.4  
At2g03340.1 68415.m00293 WRKY family transcription factor contai...    28   6.4  
At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox...    28   6.4  
At5g32613.1 68418.m03881 zinc knuckle (CCHC-type) family protein...    28   8.5  
At2g35890.1 68415.m04406 calcium-dependent protein kinase, putat...    28   8.5  

>At2g45540.1 68415.m05663 WD-40 repeat family protein / beige-related
            contains Pfam PF02138: Beige/BEACH domain; contains Pfam
            PF00400: WD domain, G-beta repeat (3 copies)
          Length = 2946

 Score = 31.9 bits (69), Expect = 0.52
 Identities = 16/32 (50%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
 Frame = +1

Query: 124  VTGTCDAKFLIW-YH*AVTSRTCATESAEGSG 216
            VTG+ D+  L+W  H A TSRT  +E + GSG
Sbjct: 2684 VTGSRDSTVLLWRIHKAFTSRTSVSEPSTGSG 2715


>At5g42370.1 68418.m05159 expressed protein
          Length = 447

 Score = 29.9 bits (64), Expect = 2.1
 Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
 Frame = -2

Query: 609 PFYRIPWNSNAQAEKKTLPGPLGGVFRPL-WVTPSTL 502
           P Y +  +   Q+ +K +P PL  + R L W TPST+
Sbjct: 285 PLYDVTSSGLVQSVEKVVPRPLRSIVRLLFWYTPSTM 321


>At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to
           autophagy 7 [Arabidopsis thaliana] GI:19912147; contains
           Pfam profile PF00899: ThiF family
          Length = 697

 Score = 28.7 bits (61), Expect = 4.9
 Identities = 13/45 (28%), Positives = 25/45 (55%)
 Frame = -1

Query: 670 PQRVSGHRRKCGALRVPNHISLL*DSMELERSGRKENSSRTSRRR 536
           P  ++G    CG  +V NH++LL +S+ L+      ++S  +R +
Sbjct: 42  PISITGFYGPCGHPQVSNHLTLLSESLPLDEQSLIASTSHGNRNK 86


>At5g19700.1 68418.m02343 MATE efflux protein-related contains Pfam
           profile PF01554: Uncharacterized membrane protein family
          Length = 508

 Score = 28.7 bits (61), Expect = 4.9
 Identities = 11/30 (36%), Positives = 19/30 (63%)
 Frame = -2

Query: 546 LGGVFRPLWVTPSTLVFKDEGTIIETVPLP 457
           + G+ +P W  PS+  FKD G ++ T+ +P
Sbjct: 239 IAGLHQPTWTRPSSECFKDWGPVV-TLAIP 267


>At2g19150.1 68415.m02235 pectinesterase family protein contains
           Pfam profile: PF01095 pectinesterase
          Length = 339

 Score = 28.7 bits (61), Expect = 4.9
 Identities = 13/34 (38%), Positives = 19/34 (55%)
 Frame = +1

Query: 640 ICGGVRILSADLENSGEGCTWRCRAGSYPYPQQV 741
           I GG+  +    ++  EGCT + R G YP P +V
Sbjct: 185 ISGGIDFIFGGAQSIFEGCTLKLRVGIYP-PNEV 217


>At3g29033.1 68416.m03629 glycine-rich protein 
          Length = 167

 Score = 28.3 bits (60), Expect = 6.4
 Identities = 16/47 (34%), Positives = 21/47 (44%)
 Frame = -2

Query: 258 YPKICTDGGSKRLTPRPFCALRRARPTRYGLMIPN*KFSITRAGNGS 118
           Y  I  D  S+  +P P        P RYG + PN +F   +AG  S
Sbjct: 12  YGFIAGDDDSQSSSPSPGHGQNSPSPRRYGQVTPNPRFHGYQAGGSS 58


>At2g03340.1 68415.m00293 WRKY family transcription factor contains
           Pfam profile: PF03106 WRKY DNA -binding domain
          Length = 513

 Score = 28.3 bits (60), Expect = 6.4
 Identities = 14/29 (48%), Positives = 19/29 (65%)
 Frame = +2

Query: 485 PSSLKTSVDGVTQSGLKTPPRGPGRVFFS 571
           PS LK+S  GV++  +  PPR  G +FFS
Sbjct: 9   PSKLKSST-GVSRPTISLPPRPFGEMFFS 36


>At1g72520.1 68414.m08386 lipoxygenase, putative similar to
           lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140
           [Lycopersicon esculentum], GB:CAB56692 [Arabidopsis
           thaliana]
          Length = 926

 Score = 28.3 bits (60), Expect = 6.4
 Identities = 14/33 (42%), Positives = 19/33 (57%)
 Frame = -3

Query: 665 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQK 567
           +S +  P  R    ++PYLPS    G RTLR+K
Sbjct: 215 QSQKDHPSKRILFTNQPYLPSETPSGLRTLREK 247


>At5g32613.1 68418.m03881 zinc knuckle (CCHC-type) family protein
           contains Pfam domain, PF00098: Zinc knuckle
          Length = 457

 Score = 27.9 bits (59), Expect = 8.5
 Identities = 15/40 (37%), Positives = 22/40 (55%)
 Frame = -2

Query: 582 NAQAEKKTLPGPLGGVFRPLWVTPSTLVFKDEGTIIETVP 463
           N+  ++ T P P      PLW+TPST  F ++GT +   P
Sbjct: 145 NSSFDRITNPSPTSSK-GPLWMTPST--FLEDGTPMVVAP 181


>At2g35890.1 68415.m04406 calcium-dependent protein kinase, putative
           / CDPK, putative similar to calcium-dependent protein
           kinase, isoform AK1 (CDPK). [Arabidopsis thaliana]
           SWISS-PROT:Q06850; contains protein kinase domain,
           Pfam:PF00069; contains EF hand domain (calcium-binding
           EF-hand), Pfam:PF00036, INTERPRO:IPR002048
          Length = 520

 Score = 27.9 bits (59), Expect = 8.5
 Identities = 11/36 (30%), Positives = 18/36 (50%)
 Frame = +1

Query: 4   MTAGRWPWKSESAKECATTHLPKQPALKMDGAEAFC 111
           +T+  WP  SESAK+     L + P  ++   +  C
Sbjct: 351 LTSDPWPQVSESAKDLIRKMLERNPIQRLTAQQVLC 386


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,986,102
Number of Sequences: 28952
Number of extensions: 433396
Number of successful extensions: 1241
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1240
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1853336000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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