Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS30050
(735 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g43330.1 68415.m05388 sugar transporter family protein simila... 32 0.34
At5g13000.1 68418.m01490 glycosyl transferase family 48 protein ... 30 1.4
At5g27690.1 68418.m03321 heavy-metal-associated domain-containin... 30 1.8
At1g60160.1 68414.m06777 potassium transporter family protein si... 30 1.8
At5g56840.1 68418.m07092 DNA-binding family protein contains Pfa... 29 2.4
At3g24420.1 68416.m03065 hydrolase, alpha/beta fold family prote... 29 2.4
At2g44200.1 68415.m05500 expressed protein 29 2.4
At1g73450.1 68414.m08503 protein kinase, putative similar to nuc... 29 2.4
At2g26410.1 68415.m03169 calmodulin-binding family protein simil... 29 3.2
At1g10510.1 68414.m01183 leucine-rich repeat family protein simi... 29 4.2
At5g09400.1 68418.m01089 potassium transporter family protein si... 28 7.4
At2g43160.3 68415.m05361 epsin N-terminal homology (ENTH) domain... 28 7.4
At2g43160.2 68415.m05360 epsin N-terminal homology (ENTH) domain... 28 7.4
At2g43160.1 68415.m05359 epsin N-terminal homology (ENTH) domain... 28 7.4
At5g24260.1 68418.m02854 prolyl oligopeptidase family protein si... 27 9.8
At4g27640.1 68417.m03973 importin beta-2 subunit family protein ... 27 9.8
At4g04760.1 68417.m00698 sugar transporter family protein simila... 27 9.8
>At2g43330.1 68415.m05388 sugar transporter family protein similar
to SP|Q96QE2 Proton myo-inositol co-transporter (Hmit)
[Homo sapiens], SP|Q01440 Membrane transporter D1
{Leishmania donovani}; contains Pfam profile PF00083:
major facilitator superfamily protein
Length = 509
Score = 32.3 bits (70), Expect = 0.34
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +2
Query: 38 PGLAAALILLFLPESPKFLLANKGPEAALPVLATMYE 148
P + +++LF+PESP++L A+ VLA Y+
Sbjct: 200 PAVIQFILMLFMPESPRWLFMKNRKAEAIQVLARTYD 236
>At5g13000.1 68418.m01490 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1889
Score = 30.3 bits (65), Expect = 1.4
Identities = 18/48 (37%), Positives = 24/48 (50%), Gaps = 5/48 (10%)
Frame = +3
Query: 303 YVCRFYGIEWSVRV-----GTGYPQQYSARLDRSEHNHMPDYIG*IPK 431
Y+CRFY E + R+ G G Q +A L R E H P +G + K
Sbjct: 64 YLCRFYAFEKAHRLDPTSSGRGVRQFKTALLQRLEREHDPTLMGRVKK 111
>At5g27690.1 68418.m03321 heavy-metal-associated domain-containing
protein very low similarity to copper homeostasis factor
from Arabidopsis thaliana [gi:3168840]; contains Pfam
heavy metal associated domain PF00403
Length = 352
Score = 29.9 bits (64), Expect = 1.8
Identities = 15/37 (40%), Positives = 22/37 (59%)
Frame = -1
Query: 252 AMSFTAPTNPPTLWASKFDVDILPTGYFNPDP*FHSY 142
A +TAP + PT S + + + TGY +P P F+SY
Sbjct: 297 ASYYTAPPSHPT---SYYSYEYVDTGYESPPPEFYSY 330
>At1g60160.1 68414.m06777 potassium transporter family protein
similar to potassium transporter HAK2p [Mesembryanthemum
crystallinum] gi|14091471|gb|AAK53759; KUP/HAK/KT
Transporter family member, PMID:11500563; contains Pfam
profile PF02705: K+ potassium transporter
Length = 827
Score = 29.9 bits (64), Expect = 1.8
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = +2
Query: 131 LATMYEWNYGSGLKYPVGRISTSNLDAQR-VGGFVGAVK 244
L MY WNYGS LKY ++D R +G +G ++
Sbjct: 552 LTVMYIWNYGSVLKYQSEVRERISMDFMRELGSTLGTIR 590
>At5g56840.1 68418.m07092 DNA-binding family protein contains Pfam
domains, PF00249: Myb-like DNA-binding domain and
PF00098: Zinc knuckle
Length = 233
Score = 29.5 bits (63), Expect = 2.4
Identities = 16/37 (43%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = -2
Query: 284 THLRRGGLNIGQCPSQLRRILQLFG-HLNLTSISFPP 177
+H G N C S R+++LFG HL+ TS S PP
Sbjct: 6 SHCGNVGHNSRTCSSYQTRVVRLFGVHLDTTSSSPPP 42
>At3g24420.1 68416.m03065 hydrolase, alpha/beta fold family protein
low similarity to 3-oxoadipate enol-lactone hydrolase
[Pseudomonas sp. B13] GI:17736948, B-ketoadipate
enol-lactone hydrolase [Bradyrhizobium japonicum]
GI:2239060; contains Pfam profile:PF00561
abhydrolase:alpha/beta hydrolase fold
Length = 273
Score = 29.5 bits (63), Expect = 2.4
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 2/52 (3%)
Frame = +2
Query: 227 FVGAVKD--IAQCSSHPSLDVFSSHIHLCLPFLWYRVVCTCGYRISSTVFCA 376
F GA+KD + S + SLDVFS + + L + V G+ +S + CA
Sbjct: 59 FSGAIKDQTLYDPSKYNSLDVFSDDLIALMEELKFGPVVFVGHSMSGVIGCA 110
>At2g44200.1 68415.m05500 expressed protein
Length = 493
Score = 29.5 bits (63), Expect = 2.4
Identities = 21/70 (30%), Positives = 30/70 (42%)
Frame = -2
Query: 722 EHVDNNGS*YRGRTS*AQRDYEQGGGSPTVGHERCSHRGNTTDEHYSTVKGLSSDLVDEE 543
+H +G + +S + D E+ G G R SH T+ H + SDL DE
Sbjct: 183 KHKRKSGKHQKQSSSRQRSDSEEDSGEENNG--RKSHHQKTSGTHDRHYERPRSDLEDES 240
Query: 542 ADGEVRDREH 513
E RDR +
Sbjct: 241 KGRESRDRHY 250
>At1g73450.1 68414.m08503 protein kinase, putative similar to
nuclear serine/threonine protein kinase GI:3582644 from
[Rattus norvegicus]
Length = 1152
Score = 29.5 bits (63), Expect = 2.4
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +2
Query: 68 FLPESPKFLLANKGPEAALPVLATMYEWNYGSGLKYPVGRISTSN 202
F+P F + +AA P A YE+ G+G P G I ++
Sbjct: 110 FVPSEMSFTFSKNSGDAAAPPDAHSYEFTSGNGTLEPYGNIDDNS 154
>At2g26410.1 68415.m03169 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 516
Score = 29.1 bits (62), Expect = 3.2
Identities = 15/37 (40%), Positives = 18/37 (48%), Gaps = 2/37 (5%)
Frame = -1
Query: 348 YPHVQTTRYHKNGKHRCM*DENTSKEG--WLEHWAMS 244
Y H QT R H+ + D NT+ G WLE W S
Sbjct: 264 YSHQQTWRNSSKLPHQTLMDTNTTDWGWSWLERWMAS 300
>At1g10510.1 68414.m01183 leucine-rich repeat family protein similar
to ribonuclease inhibitor (GI:164639) [Sus scrofa
(pig)]; contains Pfam PF00560: Leucine Rich Repeat
domains
Length = 605
Score = 28.7 bits (61), Expect = 4.2
Identities = 9/31 (29%), Positives = 19/31 (61%)
Frame = +3
Query: 321 GIEWSVRVGTGYPQQYSARLDRSEHNHMPDY 413
G++WS+ GT Q ++A++DR + ++
Sbjct: 135 GVKWSIGAGTNLLQGFAAKVDREAKQRLNEF 165
>At5g09400.1 68418.m01089 potassium transporter family protein
similar to K+ transporter HAK5 [Arabidopsis thaliana]
GI:7108597; contains Pfam profile PF02705: K+ potassium
transporter; KUP/HAK/KT Transporter family member,
PMID:11500563; Note: possible sequencing error causes a
frameshift in the 4th exon|15810448|gb|AY056263
Length = 858
Score = 27.9 bits (59), Expect = 7.4
Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = +2
Query: 119 ALPVLATMYEWNYGSGLKYPVGRISTSNLDAQR-VGGFVGAVK 244
A+ + MY WNYGS L+Y ++D R +G +G ++
Sbjct: 564 AVIMFGIMYIWNYGSKLRYETEVEQKLSMDLMRELGCNLGTIR 606
>At2g43160.3 68415.m05361 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Af10-protein (GI:1724114) [Avena fatua]
Length = 895
Score = 27.9 bits (59), Expect = 7.4
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -3
Query: 688 AAHPEHSETMSRAVAAPPWGTNAAPTVAT 602
AA P + + A+PP GTN A T AT
Sbjct: 320 AASPPPPQVAAPEAASPPTGTNTANTTAT 348
>At2g43160.2 68415.m05360 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Af10-protein (GI:1724114) [Avena fatua]
Length = 895
Score = 27.9 bits (59), Expect = 7.4
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -3
Query: 688 AAHPEHSETMSRAVAAPPWGTNAAPTVAT 602
AA P + + A+PP GTN A T AT
Sbjct: 320 AASPPPPQVAAPEAASPPTGTNTANTTAT 348
>At2g43160.1 68415.m05359 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Af10-protein (GI:1724114) [Avena fatua]
Length = 895
Score = 27.9 bits (59), Expect = 7.4
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -3
Query: 688 AAHPEHSETMSRAVAAPPWGTNAAPTVAT 602
AA P + + A+PP GTN A T AT
Sbjct: 320 AASPPPPQVAAPEAASPPTGTNTANTTAT 348
>At5g24260.1 68418.m02854 prolyl oligopeptidase family protein
similar to dipeptidyl peptidase IV [Stenotrophomonas
maltophilia] GI:1753197; contains Pfam profiles PF00326:
prolyl oligopeptidase family, PF00930: Dipeptidyl
peptidase IV (DPP IV) N-terminal region
Length = 746
Score = 27.5 bits (58), Expect = 9.8
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -2
Query: 653 GGGSPTVGHERCSHRGNTTDEHYSTVKGLSSDLV 552
GGG T + C RGNT DE+ V L +++
Sbjct: 262 GGGKTTWMNLVCGGRGNTEDEYLGRVNWLPGNVL 295
>At4g27640.1 68417.m03973 importin beta-2 subunit family protein low
similarity to importin 4 GI:18700635 from [Homo sapiens]
Length = 1048
Score = 27.5 bits (58), Expect = 9.8
Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = -2
Query: 626 ERCSHRGNTTDEHYSTV-KGLSSDLVDEEADGEVRDREHGAMA 501
E C + G T +++ V +G+S D E D VRD GA A
Sbjct: 891 ELCKNGGETALKYFGDVLRGISPLFGDSEPDLAVRDNAAGATA 933
>At4g04760.1 68417.m00698 sugar transporter family protein similar
to sugar-porter family proteins 1 and 2 [Arabidopsis
thaliana] GI:14585699, GI:14585701; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 467
Score = 27.5 bits (58), Expect = 9.8
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 32 CAPGLAAALILLFLPESPKFL 94
C P L +L F+PESP++L
Sbjct: 182 CIPSLMVLPLLFFIPESPRWL 202
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,133,783
Number of Sequences: 28952
Number of extensions: 422611
Number of successful extensions: 1370
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1308
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1370
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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