Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP01_F_G06
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to... 69 1e-12
At5g37600.1 68418.m04529 glutamine synthetase, putative similar ... 65 3e-11
At5g16570.1 68418.m01939 glutamine synthetase, putative similar ... 64 7e-11
At1g66200.1 68414.m07514 glutamine synthetase, putative similar ... 64 7e-11
At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to... 62 2e-10
At1g48470.1 68414.m05418 glutamine synthetase, putative similar ... 62 2e-10
At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive eff... 29 1.4
At2g41590.1 68415.m05139 expressed protein similar to zinc finge... 29 2.5
At4g11540.1 68417.m01851 DC1 domain-containing protein contains ... 28 3.3
At4g32880.1 68417.m04679 homeobox-leucine zipper transcription f... 28 4.4
At5g19280.1 68418.m02298 kinase associated protein phosphatase (... 27 5.8
At1g53180.1 68414.m06027 expressed protein 27 5.8
At1g32880.1 68414.m04051 importin alpha-1 subunit, putative simi... 27 5.8
At3g63500.2 68416.m07153 expressed protein 27 7.7
At3g63500.1 68416.m07152 expressed protein 27 7.7
At1g18550.1 68414.m02314 kinesin motor protein-related contains ... 27 7.7
>At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to
glutamine synthetase, chloroplast precursor (glutamate--
ammonia ligase, GS2) [Arabidopsis thaliana]
SWISS-PROT:Q43127
Length = 430
Score = 69.3 bits (162), Expect = 1e-12
Identities = 38/94 (40%), Positives = 53/94 (56%), Gaps = 1/94 (1%)
Frame = +3
Query: 165 KIEDNPKILSGPVLT-NSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDR 341
K + N K+ VL S N+ +++ D +D+I+A YIWI GSG LR K R
Sbjct: 37 KKQSNNKVRGFRVLALQSDNSTVNRVETLLNLDTKPYSDRIIAEYIWIGGSGIDLRSKSR 96
Query: 342 TLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYL 443
T+ + P +LP WN+DGSST QA G +S+ L
Sbjct: 97 TIEKPVEDPSELPKWNYDGSSTGQAPGEDSEVIL 130
Score = 33.1 bits (72), Expect = 0.12
Identities = 20/46 (43%), Positives = 27/46 (58%)
Frame = +1
Query: 361 KLPKICLYGTSMAAQPTKLMGTILIPTSXPRAIYKDPFRRGNHILV 498
+LPK G+S P + IL P +AI++DPFR GN+ILV
Sbjct: 107 ELPKWNYDGSSTGQAPGEDSEVILYP----QAIFRDPFRGGNNILV 148
>At5g37600.1 68418.m04529 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 64.9 bits (151), Expect = 3e-11
Identities = 30/56 (53%), Positives = 36/56 (64%)
Frame = +3
Query: 276 DKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYL 443
DKI+A YIW+ GSG +R K RTL P LP WN+DGSST QA G +S+ L
Sbjct: 17 DKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGEDSEVIL 72
Score = 37.1 bits (82), Expect = 0.007
Identities = 22/46 (47%), Positives = 28/46 (60%)
Frame = +1
Query: 361 KLPKICLYGTSMAAQPTKLMGTILIPTSXPRAIYKDPFRRGNHILV 498
+LPK G+S P + IL P +AI+KDPFRRGN+ILV
Sbjct: 49 QLPKWNYDGSSTGQAPGEDSEVILYP----QAIFKDPFRRGNNILV 90
>At5g16570.1 68418.m01939 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase) [Alfalfa] SWISS-PROT:P04078
Length = 356
Score = 63.7 bits (148), Expect = 7e-11
Identities = 31/62 (50%), Positives = 38/62 (61%)
Frame = +3
Query: 258 DLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDT 437
DL D+I+A YIWI GSG +R K RTL P LP WN+DGSST QA G +S+
Sbjct: 11 DLSDSTDQIIAEYIWIGGSGLDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGDDSEV 70
Query: 438 YL 443
+
Sbjct: 71 II 72
Score = 35.5 bits (78), Expect = 0.022
Identities = 14/17 (82%), Positives = 17/17 (100%)
Frame = +1
Query: 448 PRAIYKDPFRRGNHILV 498
P+AI+KDPFRRGN+ILV
Sbjct: 74 PQAIFKDPFRRGNNILV 90
>At1g66200.1 68414.m07514 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 63.7 bits (148), Expect = 7e-11
Identities = 30/62 (48%), Positives = 39/62 (62%)
Frame = +3
Query: 258 DLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDT 437
D+ ++KI+A YIW+ GSG +R K RTL P LP WN+DGSST QA G +S+
Sbjct: 11 DISDNSEKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSKLPKWNYDGSSTGQAPGQDSEV 70
Query: 438 YL 443
L
Sbjct: 71 IL 72
Score = 38.7 bits (86), Expect = 0.002
Identities = 23/46 (50%), Positives = 28/46 (60%)
Frame = +1
Query: 361 KLPKICLYGTSMAAQPTKLMGTILIPTSXPRAIYKDPFRRGNHILV 498
KLPK G+S P + IL P +AI+KDPFRRGN+ILV
Sbjct: 49 KLPKWNYDGSSTGQAPGQDSEVILYP----QAIFKDPFRRGNNILV 90
>At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 354
Score = 62.1 bits (144), Expect = 2e-10
Identities = 30/55 (54%), Positives = 35/55 (63%)
Frame = +3
Query: 279 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYL 443
KI+A YIWI GSG +R K RTL P LP WN+DGSST QA G +S+ L
Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVTDPSKLPKWNYDGSSTGQAAGEDSEVIL 72
Score = 34.3 bits (75), Expect = 0.050
Identities = 13/17 (76%), Positives = 17/17 (100%)
Frame = +1
Query: 448 PRAIYKDPFRRGNHILV 498
P+AI+KDPFR+GN+ILV
Sbjct: 74 PQAIFKDPFRKGNNILV 90
>At1g48470.1 68414.m05418 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 353
Score = 62.1 bits (144), Expect = 2e-10
Identities = 30/55 (54%), Positives = 36/55 (65%)
Frame = +3
Query: 279 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYL 443
KI+A YIWI GSG +R K RTL P LP WN+DGSST+QA G +S+ L
Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVSNPTKLPKWNYDGSSTDQAAGDDSEVIL 72
Score = 34.3 bits (75), Expect = 0.050
Identities = 13/17 (76%), Positives = 17/17 (100%)
Frame = +1
Query: 448 PRAIYKDPFRRGNHILV 498
P+AI+KDPFR+GN+ILV
Sbjct: 74 PQAIFKDPFRKGNNILV 90
>At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 638
Score = 29.5 bits (63), Expect = 1.4
Identities = 17/80 (21%), Positives = 39/80 (48%)
Frame = -2
Query: 253 YRLSSVLDSTAFGELVKTGPDRIFGLSSILVCAVSAILKQVRHDYVLKCSLDASVEYCAK 74
+ + +L+ + + +KT PD+ ++ L C V +L++ R + L S + +K
Sbjct: 16 WSMEDILNKDLYKQKIKTIPDKFSSVNEYLKCFVPHLLEETRTE------LSTSFKSLSK 69
Query: 73 TYLRKVLSERKMFRSPHGAV 14
+L ++ S M + G++
Sbjct: 70 AHLFEISSLETMTKESSGSL 89
>At2g41590.1 68415.m05139 expressed protein similar to zinc finger
protein [Arabidopsis thaliana] GI:976277
Length = 367
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +3
Query: 249 RYNDLPLPADKILATYIWID-GSGEHLRCKDRTLNF 353
R + PL D+ +A Y +D G +H+RC+ + NF
Sbjct: 263 RISHPPLNHDEFVAAYPHLDSGRNDHIRCEGESSNF 298
>At4g11540.1 68417.m01851 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 525
Score = 28.3 bits (60), Expect = 3.3
Identities = 19/63 (30%), Positives = 28/63 (44%)
Frame = -3
Query: 432 QNCAHQLGWLSCHRSSI*ADLWEL*V*NSRCDLCTSGVRQSRRSKCRWRESCQPAVEDHC 253
Q+ +H+ +L H + + LWE N RC CT VR C+ C + HC
Sbjct: 286 QHFSHKEHYLRFHVNGL---LWEE---NKRCSACTHPVRLQSFYACK---DCDFILHQHC 336
Query: 252 IDS 244
+S
Sbjct: 337 AES 339
>At4g32880.1 68417.m04679 homeobox-leucine zipper transcription
factor (HB-8) identical to HD-zip transcription factor
(athb-8) (GI:7270235) [Arabidopsis thaliana]
Length = 833
Score = 27.9 bits (59), Expect = 4.4
Identities = 14/34 (41%), Positives = 17/34 (50%)
Frame = +2
Query: 296 HLDRRLWRTPEVQRSHLEFHT*SSQRSAYMELRW 397
H D W PEV RS E T +QR+ LR+
Sbjct: 332 HFDLEPWSVPEVLRSLYESSTLLAQRTTMAALRY 365
>At5g19280.1 68418.m02298 kinase associated protein phosphatase
(KAPP) identical to Kinase associated protein
phosphatase (SP:P46014) [Arabidopsis thaliana]; contains
Pfam PF00481: Protein phosphatase 2C domain; contains
Pfam PF00498: FHA domain
Length = 581
Score = 27.5 bits (58), Expect = 5.8
Identities = 18/51 (35%), Positives = 26/51 (50%), Gaps = 7/51 (13%)
Frame = +3
Query: 261 LPLPADK----ILATYIWIDGSGEHLRC---KDRTLNFIPKAPKDLPIWNF 392
L LPA+K + TY W + E+L+ KDR +N P+ +D W F
Sbjct: 133 LKLPAEKGSLAEVQTYDWQNNRNENLQYNLEKDRLINLSPRLVEDQRSWLF 183
>At1g53180.1 68414.m06027 expressed protein
Length = 358
Score = 27.5 bits (58), Expect = 5.8
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = -2
Query: 319 SPEPSIQM*VARILSAGSGRSLYRLSSVLDSTAFGELVK 203
SPE + ++L A +GRSL R SS+ DS G K
Sbjct: 49 SPEKMGDSDIGKVLEASTGRSLIRASSI-DSREGGSQTK 86
>At1g32880.1 68414.m04051 importin alpha-1 subunit, putative similar
to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 183
Score = 27.5 bits (58), Expect = 5.8
Identities = 17/59 (28%), Positives = 26/59 (44%)
Frame = -2
Query: 292 VARILSAGSGRSLYRLSSVLDSTAFGELVKTGPDRIFGLSSILVCAVSAILKQVRHDYV 116
V R++ S +L SV+D+ LVK + F + VCA+S HD +
Sbjct: 26 VHRLIQFLLDESFPKLQSVIDANLIPTLVKLTQNAEFDMKKESVCAISNATLLGSHDQI 84
>At3g63500.2 68416.m07153 expressed protein
Length = 1162
Score = 27.1 bits (57), Expect = 7.7
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = -3
Query: 90 LNIARKRIYERFCRSVKCFDRLTGRSRD 7
LN AR+ +ERFC+ ++ +++ S+D
Sbjct: 911 LNFAREWKFERFCKELEYVNKIFSSSKD 938
>At3g63500.1 68416.m07152 expressed protein
Length = 887
Score = 27.1 bits (57), Expect = 7.7
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = -3
Query: 90 LNIARKRIYERFCRSVKCFDRLTGRSRD 7
LN AR+ +ERFC+ ++ +++ S+D
Sbjct: 636 LNFAREWKFERFCKELEYVNKIFSSSKD 663
>At1g18550.1 68414.m02314 kinesin motor protein-related contains
similarity to kinesin-related protein GI:4493964 from
[Plasmodium falciparum]
Length = 799
Score = 27.1 bits (57), Expect = 7.7
Identities = 26/76 (34%), Positives = 36/76 (47%), Gaps = 5/76 (6%)
Frame = -2
Query: 349 FKVRSLHLRCSPEPSIQM*VARIL-SAGSGRSLYRLSSVLDSTAFGELVKTG----PDRI 185
F +R+ + SP+P Q ++L S+ G +RL DST E K DRI
Sbjct: 52 FSLRNPNHNQSPKPEDQRFKTQLLDSSKKGDRFHRLDGK-DSTFVEEDTKENNVFEADRI 110
Query: 184 FGLSSILVCAVSAILK 137
FG+SS+ V I K
Sbjct: 111 FGVSSVPVKPSGVIRK 126
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,204,207
Number of Sequences: 28952
Number of extensions: 264950
Number of successful extensions: 652
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 636
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 652
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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