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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fprWP01_F_F24
         (656 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) ...    93   1e-19
At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical...    93   2e-19
At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) ...    76   2e-14
At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) ...    61   6e-10
At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 ...    57   9e-09
At5g48700.1 68418.m06027 ubiquitin-related contains similarity t...    52   4e-07
At5g55855.1 68418.m06962 hypothetical protein                          45   5e-05
At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-...    43   2e-04

>At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO)
           similar to ubiquitin-like protein SMT3 SP:P55852 from
           [Arabidopsis thaliana]; identical to cDNA small
           ubiquitin-like modifier 2 (SUMO)  GI:22652843; contains
           Pfam profile PF00240: Ubiquitin family
          Length = 103

 Score = 93.5 bits (222), Expect = 1e-19
 Identities = 43/85 (50%), Positives = 54/85 (63%)
 Frame = +3

Query: 126 DEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPIN 305
           D+K  +  HINLKV GQD   V F+IK+ T L+KLMNAYCDR  +    + F FDG+ + 
Sbjct: 8   DKKPDQGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDFNSIAFLFDGRRLR 67

Query: 306 ENDTPTSLEMEEGDTIEVYQQQTGG 380
              TP  LEME+GD I+    QTGG
Sbjct: 68  AEQTPDELEMEDGDEIDAMLHQTGG 92


>At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to
           Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis
           thaliana]; identical to cDNA SMT3 protein GI:1707371
          Length = 100

 Score = 92.7 bits (220), Expect = 2e-19
 Identities = 44/86 (51%), Positives = 56/86 (65%), Gaps = 1/86 (1%)
 Frame = +3

Query: 126 DEKKGEN-EHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPI 302
           D+K G+   HINLKV GQD   V F+IK+ T L+KLMNAYCDR  + M  + F FDG+ +
Sbjct: 8   DKKPGDGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMNSIAFLFDGRRL 67

Query: 303 NENDTPTSLEMEEGDTIEVYQQQTGG 380
               TP  L+ME+GD I+    QTGG
Sbjct: 68  RAEQTPDELDMEDGDEIDAMLHQTGG 93


>At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO)
           similar to SP|O13351 Ubiquitin-like protein smt3/pmt3
           {Schizosaccharomyces pombe}; identical to cDNA small
           ubiquitin-like modifier 3 (SUMO) GI:22652845
          Length = 111

 Score = 76.2 bits (179), Expect = 2e-14
 Identities = 36/81 (44%), Positives = 48/81 (59%)
 Frame = +3

Query: 141 ENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPINENDTP 320
           +  H+ LKV  QD   V FK KK  PL+KLM  YCDR GL +    F F+G  I   +TP
Sbjct: 14  QEAHVILKVKSQDGDEVLFKNKKSAPLKKLMYVYCDRRGLKLDAFAFIFNGARIGGLETP 73

Query: 321 TSLEMEEGDTIEVYQQQTGGV 383
             L+ME+GD I+  +  +GG+
Sbjct: 74  DELDMEDGDVIDACRAMSGGL 94


>At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO)
           similar to ubiquitin-like protein SMT3 SP:P55852
           [Arabidopsis thaliana]; contains INTERPRO:IPR000626
           ubiquitin domain; contains Pfam profile PF00240:
           Ubiquitin family; contains Pfam profile PF00240:
           Ubiquitin family;  identical to cDNA small
           ubiquitin-like modifier 5 (SUMO) mRNA GI:22652847
          Length = 108

 Score = 61.3 bits (142), Expect = 6e-10
 Identities = 32/91 (35%), Positives = 46/91 (50%)
 Frame = +3

Query: 108 INLKMADEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRF 287
           ++ K    +   +  + LKV  Q  A   +KI  H  L+KLM+AYC +  L    VRF +
Sbjct: 13  VSKKSRSPETSPHMKVTLKVKNQQGAEDLYKIGTHAHLKKLMSAYCTKRNLDYSSVRFVY 72

Query: 288 DGQPINENDTPTSLEMEEGDTIEVYQQQTGG 380
           +G+ I    TP  L MEE D I +  +  GG
Sbjct: 73  NGREIKARQTPAQLHMEEEDEICMVMELGGG 103


>At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351
           Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces
           pombe}
          Length = 114

 Score = 57.2 bits (132), Expect = 9e-09
 Identities = 32/93 (34%), Positives = 47/93 (50%), Gaps = 4/93 (4%)
 Frame = +3

Query: 114 LKMADEKK---GENEHINLKVLGQDNAIVQ-FKIKKHTPLRKLMNAYCDRAGLSMQVVRF 281
           +KM  +K+    +  H+ LKV GQD    + F ++++  L K+M  Y    G+     RF
Sbjct: 12  VKMEGQKRKVVSDPTHVTLKVKGQDEEDFRVFWVRRNAKLLKMMELYTKMRGIEWNTFRF 71

Query: 282 RFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 380
            FDG  I E  TP  LE ++GD I+    Q  G
Sbjct: 72  LFDGSRIREYHTPDELERKDGDEIDAMLCQQSG 104


>At5g48700.1 68418.m06027 ubiquitin-related contains similarity to
           SP|O13351 Ubiquitin-like protein smt3/pmt3
           {Schizosaccharomyces pombe}
          Length = 117

 Score = 51.6 bits (118), Expect = 4e-07
 Identities = 32/95 (33%), Positives = 47/95 (49%), Gaps = 6/95 (6%)
 Frame = +3

Query: 114 LKMADEKK----GENEHINLKVLGQDNAIVQ-FKIKKHTPLRKLMNAYCDRAGLSMQVVR 278
           +KM  EK+     E+ H+ L V GQD   V+ F++++   L KLM  Y    G+     R
Sbjct: 14  VKMEGEKRKDVESESTHVTLNVKGQDEEGVKVFRVRRKARLLKLMEYYAKMRGIEWNTFR 73

Query: 279 FRFD-GQPINENDTPTSLEMEEGDTIEVYQQQTGG 380
           F  D G  I E  T   +E+++GD I+    Q  G
Sbjct: 74  FLSDDGSRIREYHTADDMELKDGDQIDALLPQESG 108


>At5g55855.1 68418.m06962 hypothetical protein
          Length = 59

 Score = 44.8 bits (101), Expect = 5e-05
 Identities = 22/56 (39%), Positives = 29/56 (51%), Gaps = 3/56 (5%)
 Frame = +3

Query: 228 LMNAYCDRAGLSMQVVRFRFDGQPINENDTPT---SLEMEEGDTIEVYQQQTGGVS 386
           +M AY D+ G  M   RF  DG  I  N TP     L++E+GD I+ +  Q  G S
Sbjct: 1   MMQAYSDKVGQQMSAFRFHCDGIRIKPNQTPNEELQLDLEDGDEIDAFVDQIAGFS 56


>At1g68185.1 68414.m07789 ubiquitin-related similar to
           ubiquitin-like protein smt3/pmt3 SP:O13351 from [Fission
           yeast]
          Length = 215

 Score = 42.7 bits (96), Expect = 2e-04
 Identities = 20/50 (40%), Positives = 32/50 (64%)
 Frame = +3

Query: 225 KLMNAYCDRAGLSMQVVRFRFDGQPINENDTPTSLEMEEGDTIEVYQQQT 374
           +++  Y D+A L  Q + F FDG  I+ + TP+ L ME+ D IEV+ ++T
Sbjct: 166 RVIKLYTDKAKLDPQNLVFIFDGDKIDPSTTPSELGMEDHDMIEVHTKKT 215


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,019,941
Number of Sequences: 28952
Number of extensions: 244172
Number of successful extensions: 507
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 499
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 507
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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