Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e96h0117
         (691 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming] alpha...   137   7e-33
At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming] alpha...   135   2e-32
At1g33420.1 68414.m04137 PHD finger family protein contains Pfam...    28   5.1  
At5g57140.1 68418.m08530 calcineurin-like phosphoesterase family...    27   8.9  
At4g21170.1 68417.m03062 pentatricopeptide (PPR) repeat-containi...    27   8.9  
At2g33640.1 68415.m04124 zinc finger (DHHC type) family protein ...    27   8.9  

>At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial, putative / succinyl-CoA
           synthetase, alpha chain, putative / SCS-alpha, putative
           similar to SP|P36967 Succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial precursor (EC 6.2.1.4)
           (Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
           {Dictyostelium discoideum}; contains Pfam profiles
           PF00549: CoA-ligase, PF02629: CoA binding domain
          Length = 341

 Score =  137 bits (331), Expect = 7e-33
 Identities = 66/113 (58%), Positives = 76/113 (67%)
 Frame = +2

Query: 218 CSGFHWKAGYLPRQQALDYGTKVVGGVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVIY 397
           C G   K G    +QA++YGTK+V GV+PKK GTEHLG PVF TV EAKA T A ASVIY
Sbjct: 57  CQGITGKNGTFHTEQAIEYGTKMVAGVTPKKGGTEHLGLPVFNTVAEAKAETKANASVIY 116

Query: 398 VPPPGXXXXXXXXXXXXMPLIVCITEGVPQHDMVRVKMPFLRQNKSRLVGPNC 556
           VP P             + LIVCITEG+PQHDMVRVK     Q+K+RL+GPNC
Sbjct: 117 VPAPFAAAAIMEGLAAELDLIVCITEGIPQHDMVRVKAALNSQSKTRLIGPNC 169



 Score = 33.5 bits (73), Expect = 0.14
 Identities = 32/139 (23%), Positives = 59/139 (42%), Gaps = 2/139 (1%)
 Frame = +3

Query: 189 LILTSETKVIVQGFTGKQGTFHANKPLTMVLKLLEECHQRR-LVQNILVSLCLVQSKRQR 365
           + +   T+VI QG TGK GTFH  + +    K++     ++   +++ + +    ++ + 
Sbjct: 47  VFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVAGVTPKKGGTEHLGLPVFNTVAEAKA 106

Query: 366 QAQXXXXXXXXXXXXXXXXPF*KLLRLKCPSLCALQKV-CHNMIWCE*KCPS*DKTSLDW 542
           + +                    L       +C  + +  H+M+  +    S  KT L  
Sbjct: 107 ETKANASVIYVPAPFAAAAIMEGLAAELDLIVCITEGIPQHDMVRVKAALNSQSKTRL-- 164

Query: 543 LVLTVPGIIAPEKCKIGIM 599
           +    PGII P +CKIGIM
Sbjct: 165 IGPNCPGIIKPGECKIGIM 183


>At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial, putative / succinyl-CoA
           synthetase, alpha chain, putative / SCS-alpha, putative
           identical to SP|P53586 Succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial precursor (EC 6.2.1.4)
           (Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
           {Arabidopsis thaliana}; strong similarity to SP|P13086
           Succinyl-CoA ligase [GDP-forming] alpha-chain,
           mitochondrial precursor {Rattus norvegicus}; contains
           Pfam profiles PF00549: CoA-ligase, PF02629: CoA binding
           domain
          Length = 347

 Score =  135 bits (327), Expect = 2e-32
 Identities = 65/113 (57%), Positives = 76/113 (67%)
 Frame = +2

Query: 218 CSGFHWKAGYLPRQQALDYGTKVVGGVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVIY 397
           C G   K G    +QA++YGTK+V GV+PKK GTEHLG PVF +V EAKA T A ASVIY
Sbjct: 62  CQGITGKNGTFHTEQAIEYGTKMVAGVTPKKGGTEHLGLPVFNSVAEAKADTKANASVIY 121

Query: 398 VPPPGXXXXXXXXXXXXMPLIVCITEGVPQHDMVRVKMPFLRQNKSRLVGPNC 556
           VP P             + LIVCITEG+PQHDMVRVK     Q+K+RL+GPNC
Sbjct: 122 VPAPFAAAAIMEGIEAELDLIVCITEGIPQHDMVRVKHALNSQSKTRLIGPNC 174



 Score = 32.3 bits (70), Expect = 0.31
 Identities = 35/142 (24%), Positives = 58/142 (40%), Gaps = 5/142 (3%)
 Frame = +3

Query: 189 LILTSETKVIVQGFTGKQGTFHANKPLTMVLKLLEECHQRR-----LVQNILVSLCLVQS 353
           + +   T+V+ QG TGK GTFH  + +    K++     ++     L   +  S+   ++
Sbjct: 52  VFVDKNTRVLCQGITGKNGTFHTEQAIEYGTKMVAGVTPKKGGTEHLGLPVFNSVAEAKA 111

Query: 354 KRQRQAQXXXXXXXXXXXXXXXXPF*KLLRLKCPSLCALQKVCHNMIWCE*KCPS*DKTS 533
             +  A                    +L  + C +    Q   H+M+  +    S  KT 
Sbjct: 112 DTKANASVIYVPAPFAAAAIMEGIEAELDLIVCITEGIPQ---HDMVRVKHALNSQSKTR 168

Query: 534 LDWLVLTVPGIIAPEKCKIGIM 599
           L  +    PGII P +CKIGIM
Sbjct: 169 L--IGPNCPGIIKPGECKIGIM 188


>At1g33420.1 68414.m04137 PHD finger family protein contains Pfam
           profile: PF00628: PHD-finger
          Length = 697

 Score = 28.3 bits (60), Expect = 5.1
 Identities = 10/32 (31%), Positives = 17/32 (53%)
 Frame = +2

Query: 545 GPNCSWYHCSREV*NWYHGQLQCTNVVXIGVV 640
           G NC W   + ++ NW + QL+    +  GV+
Sbjct: 153 GSNCKWCSMALDIENWVYSQLEDNTHLLHGVI 184


>At5g57140.1 68418.m08530 calcineurin-like phosphoesterase family
           protein contains Pfam profile: PF00149 Calcineurin-like
           phosphoesterase
          Length = 397

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = -3

Query: 104 PDWHSHCRIIECKSTKGKN 48
           P+WH   R+IE K  KG++
Sbjct: 343 PNWHRRARVIEAKLGKGRD 361


>At4g21170.1 68417.m03062 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 534

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 11/21 (52%), Positives = 13/21 (61%)
 Frame = -3

Query: 116 LRKNPDWHSHCRIIECKSTKG 54
           LR  PD  SHCR+IE  +  G
Sbjct: 97  LRFEPDLKSHCRVIEVAAESG 117


>At2g33640.1 68415.m04124 zinc finger (DHHC type) family protein
           contains Pfam profile PF01529: DHHC zinc finger domain
          Length = 565

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = -1

Query: 427 NGCCSSRGRYINNRGSCSCACLCLFDCTK 341
           +GCCS+ GR+I       C CL + DC +
Sbjct: 109 SGCCSAIGRFI-------CGCLVIQDCRR 130


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,200,193
Number of Sequences: 28952
Number of extensions: 324593
Number of successful extensions: 741
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 717
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 741
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)