Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0071
(799 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 45 5e-05
At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 42 6e-04
At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 41 0.001
At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 38 0.006
At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 38 0.006
At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 38 0.006
At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 38 0.008
At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 38 0.008
At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 36 0.024
At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4) ... 30 2.0
At3g16890.1 68416.m02159 pentatricopeptide (PPR) repeat-containi... 29 3.6
At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3) ... 29 3.6
At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /... 29 4.7
At3g07140.2 68416.m00851 GPI transamidase component Gpi16 subuni... 28 6.2
At3g07140.1 68416.m00850 GPI transamidase component Gpi16 subuni... 28 6.2
At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative... 28 6.2
>At3g15850.1 68416.m02005 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 371
Score = 45.2 bits (102), Expect = 5e-05
Identities = 24/64 (37%), Positives = 34/64 (53%)
Frame = +3
Query: 501 QILETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQIL 680
Q ++D DPH+ GF+FSH+ W + + + G +V DL P RF + Y IL
Sbjct: 190 QFCDSDRDPHSPLDGFWFSHMNW-MFDTNTITQRCGEPNNVGDLEKQPFYRFLRTTY-IL 247
Query: 681 MPLA 692
PLA
Sbjct: 248 HPLA 251
Score = 28.7 bits (61), Expect = 4.7
Identities = 22/85 (25%), Positives = 32/85 (37%), Gaps = 1/85 (1%)
Frame = +1
Query: 277 LWRVLVPFQSHVEDFYIRHIFVLVFGI-GYHSWRAQTLGTQVLKARLPLRILLTIFNTIA 453
L +L PFQ + + +V G+ G + L + K L L A
Sbjct: 114 LLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQA 173
Query: 454 FQDAVVDWARDHRMHHKY*RPMRTP 528
Q +DW HR HH++ R P
Sbjct: 174 LQGNPIDWVSTHRYHHQFCDSDRDP 198
>At3g15870.1 68416.m02007 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 292
Score = 41.5 bits (93), Expect = 6e-04
Identities = 21/64 (32%), Positives = 34/64 (53%)
Frame = +3
Query: 510 ETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQILMPL 689
ET DPH+ T+GF+FSH+ W + ++ G +V+DL P RF ++ + M
Sbjct: 116 ETQRDPHSPTQGFWFSHMAW-IFDTSSILENCGGEENVDDLVRQPFYRFLQRTVLLHMMA 174
Query: 690 ACFI 701
F+
Sbjct: 175 YSFL 178
Score = 33.9 bits (74), Expect = 0.13
Identities = 15/50 (30%), Positives = 23/50 (46%)
Frame = +1
Query: 418 LRILLTIFNTIAFQDAVVDWARDHRMHHKY*RPMRTPTTQPEDSSFPTLA 567
L L +AFQ ++W +HR HHK+ R P + + F +A
Sbjct: 85 LEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMA 134
>At1g06100.1 68414.m00639 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 40.7 bits (91), Expect = 0.001
Identities = 25/77 (32%), Positives = 38/77 (49%)
Frame = +3
Query: 501 QILETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQIL 680
Q ++D DPH+ GF+FSH+ W+ + K G +V DL+ RF KK ++
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVLWIFDTDYIREKC-GRRNNVMDLKQQWFYRFLKK--TLV 175
Query: 681 MPLACFIMPTYVPTLWG 731
+ + F Y LWG
Sbjct: 176 LHILAFWTLIY---LWG 189
>At1g06360.1 68414.m00672 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 38.3 bits (85), Expect = 0.006
Identities = 16/45 (35%), Positives = 27/45 (60%)
Frame = +3
Query: 501 QILETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLR 635
Q ++D DPH+ GF+FSH+ W+ ++ + K G +V DL+
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVWWICDTRYIKYKCGGRN-NVMDLK 162
Score = 28.3 bits (60), Expect = 6.2
Identities = 15/49 (30%), Positives = 20/49 (40%)
Frame = +1
Query: 430 LTIFNTIAFQDAVVDWARDHRMHHKY*RPMRTPTTQPEDSSFPTLAGCC 576
L F A Q +DW HR HH++ R P + E F + C
Sbjct: 95 LAYFAVFALQGDPLDWVSIHRFHHQFTDSDRDPHSPIEGFWFSHVWWIC 143
>At1g06120.1 68414.m00641 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported
by cDNA:gi_12083275_gb_AF332434.1_AF332434
Length = 299
Score = 38.3 bits (85), Expect = 0.006
Identities = 18/52 (34%), Positives = 27/52 (51%)
Frame = +3
Query: 501 QILETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 656
Q ++D DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
>At1g06090.1 68414.m00638 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]
Length = 299
Score = 38.3 bits (85), Expect = 0.006
Identities = 18/52 (34%), Positives = 27/52 (51%)
Frame = +3
Query: 501 QILETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 656
Q ++D DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
>At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to
delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from
[Arabidopsis thaliana]
Length = 307
Score = 37.9 bits (84), Expect = 0.008
Identities = 24/93 (25%), Positives = 37/93 (39%)
Frame = +1
Query: 286 VLVPFQSHVEDFYIRHIFVLVFGIGYHSWRAQTLGTQVLKARLPLRILLTIFNTIAFQDA 465
+L PF ++ +F + G+G + L + K L LL +A Q
Sbjct: 55 LLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGD 114
Query: 466 VVDWARDHRMHHKY*RPMRTPTTQPEDSSFPTL 564
+DW HR HH++ R P + E F L
Sbjct: 115 PIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHL 147
Score = 37.9 bits (84), Expect = 0.008
Identities = 18/55 (32%), Positives = 29/55 (52%)
Frame = +3
Query: 501 QILETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 665
Q +++ DPH+ GF+FSH+ W+ + K G +V DL+ RF +K
Sbjct: 127 QFTDSERDPHSPKEGFWFSHLLWIYDSAYLVSKC-GRRANVEDLKRQWFYRFLQK 180
>At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to
delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180
GI:2970034 from [Arabidopsis thaliana]
Length = 305
Score = 37.9 bits (84), Expect = 0.008
Identities = 20/62 (32%), Positives = 33/62 (53%), Gaps = 2/62 (3%)
Frame = +3
Query: 501 QILETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK--YYQ 674
Q ++D DPH+ GF+FSH+ WL + ++ G +V DL+ +F ++ Y
Sbjct: 125 QFTDSDRDPHSPNEGFWFSHLLWLFDTGY-LVEKCGRRTNVEDLKRQWYYKFLQRTVLYH 183
Query: 675 IL 680
IL
Sbjct: 184 IL 185
Score = 35.1 bits (77), Expect = 0.054
Identities = 18/47 (38%), Positives = 23/47 (48%)
Frame = +2
Query: 299 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKS*KLGCH*EYYSPY 439
F W A+ +Y GLGIT HR AH+S K+ EY+ Y
Sbjct: 57 FNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAY 103
Score = 30.7 bits (66), Expect = 1.2
Identities = 23/93 (24%), Positives = 33/93 (35%)
Frame = +1
Query: 286 VLVPFQSHVEDFYIRHIFVLVFGIGYHSWRAQTLGTQVLKARLPLRILLTIFNTIAFQDA 465
+L PF + I V G+G + L + K L +A Q
Sbjct: 53 LLAPFNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGD 112
Query: 466 VVDWARDHRMHHKY*RPMRTPTTQPEDSSFPTL 564
+DW HR HH++ R P + E F L
Sbjct: 113 PIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHL 145
>At1g06350.1 68414.m00671 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 300
Score = 36.3 bits (80), Expect = 0.024
Identities = 17/55 (30%), Positives = 29/55 (52%)
Frame = +3
Query: 501 QILETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 665
Q ++D DPH+ G FSHI W+ ++ + K G +V DL+ +F ++
Sbjct: 120 QFTDSDRDPHSPKEGLLFSHILWIFDTQYIKYKCGGRD-NVLDLKKQWFYKFLRR 173
>At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4)
identical to metal transporter Nramp4 [Arabidopsis
thaliana] gi|6468014|gb|AAF13279; member of the natural
resistance-associated macrophage protein (NRAMP) metal
transporter family, PMID:11500563
Length = 512
Score = 29.9 bits (64), Expect = 2.0
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +2
Query: 272 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 379
G GY ++ MW T+I + L + LG+ G H
Sbjct: 79 GAIAGYSLIWLLMWATAIGLLIQLLSARLGVATGRH 114
>At3g16890.1 68416.m02159 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 659
Score = 29.1 bits (62), Expect = 3.6
Identities = 21/81 (25%), Positives = 35/81 (43%), Gaps = 1/81 (1%)
Frame = +2
Query: 92 VHCFSENASPKETGGFVGIVRSRRQ*P-ARRCTAPCTSLSREETLEDCVEKCDSVLILHV 268
++C S N+ KETG F+ + R P + A + L + L + D + V
Sbjct: 327 LYCLSNNSMAKETGQFLRKIGERGYIPDSSTFNAAMSCLLKGHDLVETCRIFDGFVSRGV 386
Query: 269 GGVYGGYLFLFKAMWRTSIFA 331
+ GYL L +A+ F+
Sbjct: 387 KPGFNGYLVLVQALLNAQRFS 407
>At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3)
identical to metal transporter Nramp3 [Arabidopsis
thaliana] gi|6468012|gb|AAF13278; member of the natural
resistance-associated macrophage protein (NRAMP) metal
transporter family, PMID:11500563
Length = 509
Score = 29.1 bits (62), Expect = 3.6
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +2
Query: 272 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 379
G GY L+ MW T++ + L + LG+ G H
Sbjct: 83 GAVAGYSLLWLLMWATAMGLLVQLLSARLGVATGRH 118
>At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase [Lycopersicon esculentum] GI:7381227;
contains PF00295: Glycosyl hydrolases family 28
(polygalacturonases)
Length = 468
Score = 28.7 bits (61), Expect = 4.7
Identities = 16/58 (27%), Positives = 24/58 (41%)
Frame = +3
Query: 138 SWVLYEADANNLPEDAPPHVPPSAEKRPWKIVWRNVILFLFCMSVVFMEGTCSFSKPC 311
S+ ++ D +P D P P S KR W +R + L V+ G + PC
Sbjct: 124 SYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPC 181
>At3g07140.2 68416.m00851 GPI transamidase component Gpi16 subunit
family protein similar to phosphatidyl inositol glycan
class T (GI:14456615) [Homo sapiens]; contains Pfam
profile PF04113: Gpi16 subunit, GPI transamidase
component
Length = 643
Score = 28.3 bits (60), Expect = 6.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +3
Query: 195 VPPSAEKRPWKIVWRNVILFLFCMSVVFMEGTCSFSKPCGG 317
VP S WK + + + LFC S+ F+E + S++ P G
Sbjct: 122 VPQSQVDTSWKNL-THALSGLFCASINFLESSTSYAAPTWG 161
>At3g07140.1 68416.m00850 GPI transamidase component Gpi16 subunit
family protein similar to phosphatidyl inositol glycan
class T (GI:14456615) [Homo sapiens]; contains Pfam
profile PF04113: Gpi16 subunit, GPI transamidase
component
Length = 644
Score = 28.3 bits (60), Expect = 6.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +3
Query: 195 VPPSAEKRPWKIVWRNVILFLFCMSVVFMEGTCSFSKPCGG 317
VP S WK + + + LFC S+ F+E + S++ P G
Sbjct: 122 VPQSQVDTSWKNL-THALSGLFCASINFLESSTSYAAPTWG 161
>At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative
(NRAMP2) similar to metal transporter Nramp3
[Arabidopsis thaliana] gi|6468012|gb|AAF13278; member of
the natural resistance-associated macrophage protein
(NRAMP) metal transporter family, PMID:11500563
Length = 530
Score = 28.3 bits (60), Expect = 6.2
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = +2
Query: 272 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 379
G GY L+ MW T++ + L + +G+ G H
Sbjct: 95 GAIAGYSLLWLLMWATAMGLLIQMLSARVGVATGRH 130
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,812,254
Number of Sequences: 28952
Number of extensions: 476791
Number of successful extensions: 1345
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1271
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1342
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1804564000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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