Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0049
(746 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 142 2e-34
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 51 9e-07
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 51 9e-07
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 36 0.029
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 36 0.029
At1g49340.2 68414.m05531 phosphatidylinositol 3- and 4-kinase fa... 30 1.4
At1g49340.1 68414.m05530 phosphatidylinositol 3- and 4-kinase fa... 30 1.4
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 30 1.9
At3g09710.1 68416.m01150 calmodulin-binding family protein low s... 30 1.9
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 29 3.3
At5g45720.1 68418.m05621 hypothetical protein 28 5.7
At5g40740.1 68418.m04944 expressed protein 28 7.6
At5g24680.1 68418.m02917 expressed protein similar to unknown pr... 28 7.6
At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P329... 28 7.6
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 28 7.6
At2g39980.1 68415.m04913 transferase family protein contains Pfa... 28 7.6
At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolas... 27 10.0
At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to ... 27 10.0
At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to ... 27 10.0
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 142 bits (345), Expect = 2e-34
Identities = 61/78 (78%), Positives = 69/78 (88%)
Frame = +1
Query: 511 MVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHP 690
MVIVS ILERD H ++LWNTAV+I + GN+IGKHRKNHIPRVGDFNES YYMEG+TGHP
Sbjct: 176 MVIVSPILERDIDHGEVLWNTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHP 235
Query: 691 VFATRYGKIAVNICFGRH 744
VF T +GKIAVNIC+GRH
Sbjct: 236 VFETVFGKIAVNICYGRH 253
Score = 115 bits (276), Expect = 4e-26
Identities = 53/90 (58%), Positives = 67/90 (74%)
Frame = +2
Query: 239 PENCEVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFC 418
P VG++Q+SIA+PT P ++Q + IF+K+K IID AG GVNI+C QE W MPFAFC
Sbjct: 87 PRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTMPFAFC 146
Query: 419 TREKQPWCEFAESAEDGPTTTFLRELAIKY 508
TRE++ WCEFAE DG +T FL+ELA KY
Sbjct: 147 TRERR-WCEFAEPV-DGESTKFLQELAKKY 174
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 50.8 bits (116), Expect = 9e-07
Identities = 22/57 (38%), Positives = 34/57 (59%)
Frame = +1
Query: 565 WNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICF 735
+N+ +I G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC+
Sbjct: 131 YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICW 187
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 50.8 bits (116), Expect = 9e-07
Identities = 22/57 (38%), Positives = 34/57 (59%)
Frame = +1
Query: 565 WNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICF 735
+N+ +I G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC+
Sbjct: 104 YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICW 160
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 35.9 bits (79), Expect = 0.029
Identities = 24/69 (34%), Positives = 31/69 (44%), Gaps = 5/69 (7%)
Frame = +1
Query: 544 EKHSDILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRY 708
E+ D L+NT V G + KHRK H IP F ES G T + T
Sbjct: 176 ERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDTDV 234
Query: 709 GKIAVNICF 735
G+I + IC+
Sbjct: 235 GRIGIGICY 243
Score = 33.5 bits (73), Expect = 0.15
Identities = 19/69 (27%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = +2
Query: 299 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 472
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 473 TTTFLRELA 499
+T L E++
Sbjct: 154 STAMLSEVS 162
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 35.9 bits (79), Expect = 0.029
Identities = 24/69 (34%), Positives = 31/69 (44%), Gaps = 5/69 (7%)
Frame = +1
Query: 544 EKHSDILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRY 708
E+ D L+NT V G + KHRK H IP F ES G T + T
Sbjct: 176 ERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDTDV 234
Query: 709 GKIAVNICF 735
G+I + IC+
Sbjct: 235 GRIGIGICY 243
Score = 33.5 bits (73), Expect = 0.15
Identities = 19/69 (27%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = +2
Query: 299 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 472
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 473 TTTFLRELA 499
+T L E++
Sbjct: 154 STAMLSEVS 162
>At1g49340.2 68414.m05531 phosphatidylinositol 3- and 4-kinase family
protein contains similarity to phosphatidylinositol
4-kinase 230 GI:2326227 from [Homo sapiens], from [Bos
taurus] GI:2198791; contains Pfam profiles PF00454:
Phosphatidylinositol 3- and 4-kinase, PF00613:
Phosphoinositide 3-kinase family, accessory domain
Length = 2028
Score = 30.3 bits (65), Expect = 1.4
Identities = 11/32 (34%), Positives = 21/32 (65%)
Frame = +1
Query: 466 RADHDLPSGTRHQVPMVIVSSILERDEKHSDI 561
R D +P + +VP++I ++++RD HSD+
Sbjct: 1733 RVDSGIPLQSAAKVPIMITFNVIDRDGDHSDV 1764
>At1g49340.1 68414.m05530 phosphatidylinositol 3- and 4-kinase family
protein contains similarity to phosphatidylinositol
4-kinase 230 GI:2326227 from [Homo sapiens], from [Bos
taurus] GI:2198791; contains Pfam profiles PF00454:
Phosphatidylinositol 3- and 4-kinase, PF00613:
Phosphoinositide 3-kinase family, accessory domain
Length = 2028
Score = 30.3 bits (65), Expect = 1.4
Identities = 11/32 (34%), Positives = 21/32 (65%)
Frame = +1
Query: 466 RADHDLPSGTRHQVPMVIVSSILERDEKHSDI 561
R D +P + +VP++I ++++RD HSD+
Sbjct: 1733 RVDSGIPLQSAAKVPIMITFNVIDRDGDHSDV 1764
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 29.9 bits (64), Expect = 1.9
Identities = 21/64 (32%), Positives = 29/64 (45%)
Frame = +1
Query: 544 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 723
EK L+ TA+ S G +GKHRK +P + + PV+ T GKI
Sbjct: 127 EKDGYTLYCTALFFSPQGQFLGKHRK-VMPT--SLERCIWGQGDGSTIPVYDTPIGKIGA 183
Query: 724 NICF 735
IC+
Sbjct: 184 AICW 187
>At3g09710.1 68416.m01150 calmodulin-binding family protein low
similarity to SF16 protein [Helianthus annuus]
GI:560150; contains Pfam profile PF00612: IQ
calmodulin-binding motif
Length = 454
Score = 29.9 bits (64), Expect = 1.9
Identities = 20/66 (30%), Positives = 34/66 (51%), Gaps = 1/66 (1%)
Frame = +1
Query: 100 KSSTEFILGGGITLKSN*KNPRSLQLRRLTSTSPHTLSR-PRTSRPDPREL*SRNSSAFH 276
K+ST GG T KS+ +N + + T ++ T +R PR RP P + S++S
Sbjct: 290 KNSTNRNSQGGETAKSSNRNKLNSSTKPNTPSASSTATRNPRKKRPIPSSIKSKSSDDEA 349
Query: 277 RGAHRS 294
+ + R+
Sbjct: 350 KSSERN 355
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 29.1 bits (62), Expect = 3.3
Identities = 20/64 (31%), Positives = 29/64 (45%)
Frame = +1
Query: 544 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 723
EK L+ TA+ S G +GKHRK +P + + PV+ T GK+
Sbjct: 120 EKDGYTLYCTALFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGA 176
Query: 724 NICF 735
IC+
Sbjct: 177 AICW 180
>At5g45720.1 68418.m05621 hypothetical protein
Length = 900
Score = 28.3 bits (60), Expect = 5.7
Identities = 13/42 (30%), Positives = 19/42 (45%)
Frame = +1
Query: 580 VISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATR 705
V+ D+ IG+H +NHI + N+ N PV R
Sbjct: 9 VLKDSNGDIGEHLRNHIHLTNCIHLKNHMHNNNKQSPVLTDR 50
>At5g40740.1 68418.m04944 expressed protein
Length = 741
Score = 27.9 bits (59), Expect = 7.6
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +1
Query: 535 ERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESN 660
ER+ + ++ +++ NVIG N PRVG F SN
Sbjct: 8 ERELELESAMYTNCLLLGLDPNVIGLGASNGTPRVGLFRHSN 49
>At5g24680.1 68418.m02917 expressed protein similar to unknown
protein (pir||T38384)
Length = 399
Score = 27.9 bits (59), Expect = 7.6
Identities = 17/44 (38%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Frame = -3
Query: 519 DHHWYLMASSRRKVVVGPSSADSANSHHGCFSLVQ-NAKGMFHN 391
DHH L +S PSS+ SA+S + SLVQ K F++
Sbjct: 37 DHHLALQLASDDHSSDDPSSSSSASSSNNVASLVQLQTKSQFYS 80
>At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 224
Score = 27.9 bits (59), Expect = 7.6
Identities = 19/64 (29%), Positives = 28/64 (43%)
Frame = +1
Query: 544 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 723
EK L+ T + S G +GKHRK +P + + PV+ T GK+
Sbjct: 5 EKEGYTLYCTVLFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGA 61
Query: 724 NICF 735
IC+
Sbjct: 62 AICW 65
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 27.9 bits (59), Expect = 7.6
Identities = 19/64 (29%), Positives = 28/64 (43%)
Frame = +1
Query: 544 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 723
EK L+ T + S G +GKHRK +P + + PV+ T GK+
Sbjct: 127 EKEGYTLYCTVLFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGA 183
Query: 724 NICF 735
IC+
Sbjct: 184 AICW 187
>At2g39980.1 68415.m04913 transferase family protein contains Pfam
profile PF02458 transferase family
Length = 482
Score = 27.9 bits (59), Expect = 7.6
Identities = 12/28 (42%), Positives = 14/28 (50%)
Frame = -2
Query: 592 CH*LQPQCSKVCPNASRPFLISRTRSPL 509
CH +Q C CPN P LIS + L
Sbjct: 39 CHYIQKGCLFTCPNLPLPALISHLKHSL 66
>At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 422
Score = 27.5 bits (58), Expect = 10.0
Identities = 14/43 (32%), Positives = 19/43 (44%)
Frame = -2
Query: 640 RLSECGSCDVSRSRFRCH*LQPQCSKVCPNASRPFLISRTRSP 512
RL G C + R CH ++ SK R LI + R+P
Sbjct: 332 RLQGVGQCLIREYRMVCHVMKGDISKDFVEGCRAVLIDKDRNP 374
>At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 383
Score = 27.5 bits (58), Expect = 10.0
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +1
Query: 583 ISDTGNVIGKHRKNHIP 633
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
>At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 250
Score = 27.5 bits (58), Expect = 10.0
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +1
Query: 583 ISDTGNVIGKHRKNHIP 633
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,126,736
Number of Sequences: 28952
Number of extensions: 372616
Number of successful extensions: 1173
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1169
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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