Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0047
(689 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53850.3 68418.m06693 haloacid dehalogenase-like hydrolase fa... 79 3e-15
At5g53850.1 68418.m06692 haloacid dehalogenase-like hydrolase fa... 79 3e-15
At5g53850.2 68418.m06691 haloacid dehalogenase-like hydrolase fa... 77 1e-14
At1g79320.1 68414.m09244 latex abundant protein, putative (AMC5)... 32 0.31
At5g64030.1 68418.m08039 dehydration-responsive protein-related ... 30 1.3
At4g12010.1 68417.m01911 disease resistance protein (TIR-NBS-LRR... 29 2.2
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 29 2.9
At5g02660.1 68418.m00202 hypothetical protein contains Pfam prof... 29 3.8
At5g49900.1 68418.m06179 expressed protein contains Pfam domain ... 28 6.7
At5g61550.1 68418.m07724 protein kinase family protein contains ... 27 8.9
>At5g53850.3 68418.m06693 haloacid dehalogenase-like hydrolase
family protein low similarity to enolase-phosphatase E-1
enzyme [Klebsiella oxytoca] GI:401712; contains InterPro
accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 418
Score = 79.0 bits (186), Expect = 3e-15
Identities = 38/95 (40%), Positives = 56/95 (58%)
Frame = +3
Query: 237 QRSSERLRKLAIEDQEKSVEGLVTIPGEDASKEDQIEGLVKNVKWQMSSDRKVAPLKQLQ 416
Q + LR ED + V G V IP D KE I +V NV+ + +DRK+ LK+LQ
Sbjct: 324 QEDIKLLRAQVEEDLREGVTGAVPIPHADEGKEKVIAAMVSNVEAMIRADRKITALKELQ 383
Query: 417 GLIWKKGYDKGDIKGHVYDDVLPALEQWRSVRVRR 521
G IW+ G++ ++K V++DV ALE+W S ++R
Sbjct: 384 GHIWRTGFECDELKAIVFEDVADALEKWHSSGIKR 418
Score = 48.8 bits (111), Expect = 3e-06
Identities = 25/66 (37%), Positives = 37/66 (56%)
Frame = +1
Query: 118 LLLDIEGTTTSISFVKDKLFPYAEENVKDFLDAQWDDEDVKEAVNA*GSLLSRIRRRVLK 297
++LDIEGTTT I+FV D LFPYA ENV L+ + + +E + + + R +
Sbjct: 284 IVLDIEGTTTPITFVTDVLFPYARENVGKHLNLTYHTAETQEDIKLLRAQVEEDLREGVT 343
Query: 298 DWLPFP 315
+P P
Sbjct: 344 GAVPIP 349
>At5g53850.1 68418.m06692 haloacid dehalogenase-like hydrolase
family protein low similarity to enolase-phosphatase E-1
enzyme [Klebsiella oxytoca] GI:401712; contains InterPro
accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 402
Score = 79.0 bits (186), Expect = 3e-15
Identities = 38/95 (40%), Positives = 56/95 (58%)
Frame = +3
Query: 237 QRSSERLRKLAIEDQEKSVEGLVTIPGEDASKEDQIEGLVKNVKWQMSSDRKVAPLKQLQ 416
Q + LR ED + V G V IP D KE I +V NV+ + +DRK+ LK+LQ
Sbjct: 308 QEDIKLLRAQVEEDLREGVTGAVPIPHADEGKEKVIAAMVSNVEAMIRADRKITALKELQ 367
Query: 417 GLIWKKGYDKGDIKGHVYDDVLPALEQWRSVRVRR 521
G IW+ G++ ++K V++DV ALE+W S ++R
Sbjct: 368 GHIWRTGFECDELKAIVFEDVADALEKWHSSGIKR 402
Score = 48.8 bits (111), Expect = 3e-06
Identities = 25/66 (37%), Positives = 37/66 (56%)
Frame = +1
Query: 118 LLLDIEGTTTSISFVKDKLFPYAEENVKDFLDAQWDDEDVKEAVNA*GSLLSRIRRRVLK 297
++LDIEGTTT I+FV D LFPYA ENV L+ + + +E + + + R +
Sbjct: 268 IVLDIEGTTTPITFVTDVLFPYARENVGKHLNLTYHTAETQEDIKLLRAQVEEDLREGVT 327
Query: 298 DWLPFP 315
+P P
Sbjct: 328 GAVPIP 333
>At5g53850.2 68418.m06691 haloacid dehalogenase-like hydrolase
family protein low similarity to enolase-phosphatase E-1
enzyme [Klebsiella oxytoca] GI:401712; contains InterPro
accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 507
Score = 77.0 bits (181), Expect = 1e-14
Identities = 37/94 (39%), Positives = 55/94 (58%)
Frame = +3
Query: 237 QRSSERLRKLAIEDQEKSVEGLVTIPGEDASKEDQIEGLVKNVKWQMSSDRKVAPLKQLQ 416
Q + LR ED + V G V IP D KE I +V NV+ + +DRK+ LK+LQ
Sbjct: 308 QEDIKLLRAQVEEDLREGVTGAVPIPHADEGKEKVIAAMVSNVEAMIRADRKITALKELQ 367
Query: 417 GLIWKKGYDKGDIKGHVYDDVLPALEQWRSVRVR 518
G IW+ G++ ++K V++DV ALE+W S ++
Sbjct: 368 GHIWRTGFECDELKAIVFEDVADALEKWHSSGIK 401
Score = 67.7 bits (158), Expect = 7e-12
Identities = 33/58 (56%), Positives = 39/58 (67%)
Frame = +2
Query: 506 SEGQKIYIYSSGSVQAQKLLFGQSSAGDLLPLIDGHFDTAVGAKQEATSYTAIVEKIG 679
S G K+YIYSSGS AQKLLFG + GDL I G FDT +G K+E+ SY I E +G
Sbjct: 397 SSGIKVYIYSSGSRLAQKLLFGNTDYGDLRKYISGFFDTTIGNKKESRSYKEIKETLG 454
Score = 48.8 bits (111), Expect = 3e-06
Identities = 25/66 (37%), Positives = 37/66 (56%)
Frame = +1
Query: 118 LLLDIEGTTTSISFVKDKLFPYAEENVKDFLDAQWDDEDVKEAVNA*GSLLSRIRRRVLK 297
++LDIEGTTT I+FV D LFPYA ENV L+ + + +E + + + R +
Sbjct: 268 IVLDIEGTTTPITFVTDVLFPYARENVGKHLNLTYHTAETQEDIKLLRAQVEEDLREGVT 327
Query: 298 DWLPFP 315
+P P
Sbjct: 328 GAVPIP 333
>At1g79320.1 68414.m09244 latex abundant protein, putative (AMC5) /
caspase family protein similar to latex-abundant protein
[Hevea brasiliensis] gb:AAD13216; contains Pfam domain,
PF00656: ICE-like protease (caspase) p20 domain
Length = 368
Score = 32.3 bits (70), Expect = 0.31
Identities = 19/51 (37%), Positives = 31/51 (60%), Gaps = 1/51 (1%)
Frame = +1
Query: 100 VKK-SKVLLLDIEGTTTSISFVKDKLFPYAEENVKDFLDAQWDDEDVKEAV 249
VKK V+L +++ TTT+I V D++ E ++FL+ + D DVK A+
Sbjct: 223 VKKFMNVILSNLQETTTTIQTVSDEVLGSVENLAQEFLEQKLSD-DVKPAI 272
>At5g64030.1 68418.m08039 dehydration-responsive protein-related
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains
Pfam profile PF03141: Putative methyltransferase
Length = 829
Score = 30.3 bits (65), Expect = 1.3
Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 2/40 (5%)
Frame = +3
Query: 318 EDASKEDQIEGLVKNVKWQ--MSSDRKVAPLKQLQGLIWK 431
+DA Q+EG+VK +KW+ M+ ++ L +Q IW+
Sbjct: 778 DDAETIQQVEGMVKAMKWEVRMTYSKEKEGLLSVQKSIWR 817
>At4g12010.1 68417.m01911 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1219
Score = 29.5 bits (63), Expect = 2.2
Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
Frame = -3
Query: 147 CCSSFNIKK--QNFAFLNNIADYSILFCHFDRSTPALPELL 31
C S NI+ ++F LNN+ + + FC +S P LP+ L
Sbjct: 884 CLSGNNIENLPESFNQLNNLKWFDLKFCKMLKSLPVLPQNL 924
>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase PG1 [Glycine max]
GI:5669846; contains PF00295: Glycosyl hydrolases family
28
Length = 444
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = -2
Query: 484 GSTSSYTCPLMSPLS*PFFQISPWSCFRGATLRSDDICHLTF 359
GS +SY S +S ++SPW C T ++C TF
Sbjct: 403 GSLNSYKTWKCSDVSGTSLKVSPWPCSELRTTGGSNLCSSTF 444
>At5g02660.1 68418.m00202 hypothetical protein contains Pfam
profiles PF04780: Protein of unknown function (DUF629),
PF04781: Protein of unknown function (DUF627)
Length = 629
Score = 28.7 bits (61), Expect = 3.8
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +3
Query: 15 ILKYIKVVPAELVWICRNGKREYCNRRYC 101
+LKY+K + WICR +++ R+ C
Sbjct: 201 VLKYVKKNLKWIAWICRTCSKKFSTRQAC 229
>At5g49900.1 68418.m06179 expressed protein contains Pfam domain
PF04685: Protein of unknown function, DUF608
Length = 957
Score = 27.9 bits (59), Expect = 6.7
Identities = 14/50 (28%), Positives = 25/50 (50%)
Frame = +1
Query: 61 VEMAKENTVIGDIVKKSKVLLLDIEGTTTSISFVKDKLFPYAEENVKDFL 210
+++ +N +++K L ++ +TTS S KL EEN+ FL
Sbjct: 481 IDVPHQNDTAVSVLEKMASTLEELHASTTSNSAFGTKLLEEGEENIGHFL 530
>At5g61550.1 68418.m07724 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain;
protein kinase 1, PnPK1, Populus nigra, EMBL:AB041503
Length = 845
Score = 27.5 bits (58), Expect = 8.9
Identities = 21/73 (28%), Positives = 35/73 (47%)
Frame = +3
Query: 369 WQMSSDRKVAPLKQLQGLIWKKGYDKGDIKGHVYDDVLPALEQWRSVRVRRFTSTPLDPS 548
W +S R++A L + ++ D+ D+K D ++PALE+ R V + PS
Sbjct: 720 WPISDTRELAALGLCCTEMRRR--DRPDLK----DQIIPALERLRKVADKAQNLLSRTPS 773
Query: 549 KPRNFFLASHLLG 587
P + F+ L G
Sbjct: 774 GPPSHFICPLLKG 786
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,619,320
Number of Sequences: 28952
Number of extensions: 343773
Number of successful extensions: 1041
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1011
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1041
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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