Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0039
(715 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g12120.1 68414.m01404 expressed protein contains Pfam domain ... 31 0.57
At5g32161.1 68418.m03792 hypothetical protein 30 1.8
At3g22420.2 68416.m02830 protein kinase family protein contains ... 29 4.0
At3g22420.1 68416.m02829 protein kinase family protein contains ... 29 4.0
At1g31400.1 68414.m03845 meprin and TRAF homology domain-contain... 29 4.0
At5g45460.1 68418.m05585 expressed protein 28 7.1
At4g29200.1 68417.m04177 hypothetical protein 28 7.1
At1g72470.1 68414.m08380 exocyst subunit EXO70 family protein co... 28 7.1
At3g24800.1 68416.m03112 PRT1 protein (PRT1) E3, N-end rule ubiq... 27 9.3
At3g23900.1 68416.m03003 RNA recognition motif (RRM)-containing ... 27 9.3
>At1g12120.1 68414.m01404 expressed protein contains Pfam domain
PF05904: Plant protein of unknown function (DUF863)
Length = 483
Score = 31.5 bits (68), Expect = 0.57
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = +1
Query: 379 PTSQTTDCRNQNSTRFQLRRSTSPR 453
P S T+ C +N++R + RRS+SPR
Sbjct: 252 PASDTSCCTTENNSRIESRRSSSPR 276
>At5g32161.1 68418.m03792 hypothetical protein
Length = 193
Score = 29.9 bits (64), Expect = 1.8
Identities = 20/66 (30%), Positives = 31/66 (46%)
Frame = -1
Query: 208 PAHSTVR*NNLSTCLRIPLLRPSNGKWYSSPYTHLQRHNIDTYYRHCSPL*VLYLSHHSK 29
P HST R +NLS+ LL P +++P H + + Y S ++ HH +
Sbjct: 64 PVHSTTRSSNLSSGHSTTLLDPLVEYRFATPPDHTRPFTLPRYSTPWSS--IITHHHHHQ 121
Query: 28 SVNNSI 11
S +SI
Sbjct: 122 STGHSI 127
>At3g22420.2 68416.m02830 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 627
Score = 28.7 bits (61), Expect = 4.0
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +1
Query: 556 QET*EPEEKMHHHHIKKGRAEEKEKNTLR*RPKTMRGTRST*N*DG 693
QET EK HH+H + +E+K +++ R M G R + + DG
Sbjct: 508 QETVSSGEKSHHNHHEFDSSEDKSCSSVHGRFADMWGLRESYSDDG 553
>At3g22420.1 68416.m02829 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 568
Score = 28.7 bits (61), Expect = 4.0
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +1
Query: 556 QET*EPEEKMHHHHIKKGRAEEKEKNTLR*RPKTMRGTRST*N*DG 693
QET EK HH+H + +E+K +++ R M G R + + DG
Sbjct: 449 QETVSSGEKSHHNHHEFDSSEDKSCSSVHGRFADMWGLRESYSDDG 494
>At1g31400.1 68414.m03845 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein low similarity
to ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 278
Score = 28.7 bits (61), Expect = 4.0
Identities = 13/44 (29%), Positives = 23/44 (52%), Gaps = 4/44 (9%)
Frame = +1
Query: 100 SEGVYKDL----NTTYHWMGEEEVYGDMLKDCFNVQLSVPESSD 219
S +Y D+ +T +H + + YGD + C ++ L VP+ D
Sbjct: 21 SHAIYFDIFVVGDTKWHLLAYPKGYGDSINKCLSLFLGVPDPDD 64
>At5g45460.1 68418.m05585 expressed protein
Length = 703
Score = 27.9 bits (59), Expect = 7.1
Identities = 14/54 (25%), Positives = 25/54 (46%)
Frame = -2
Query: 186 ETIFQHVSVYLFFAHPMVSGIQVLIHTFRGTTSTHITATVLHYEFFIYHTIQNR 25
E + + + L F + + ++HT GT S + + L F I+H I N+
Sbjct: 279 EEALRIIEIELGFLYDALFTKNAVLHTVLGTVSRVVASGSLVAAFIIFHKISNK 332
>At4g29200.1 68417.m04177 hypothetical protein
Length = 457
Score = 27.9 bits (59), Expect = 7.1
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = -1
Query: 331 VLKTFCTCFLNCSSVSSTCFLKLMAF 254
VLKTF FLNC+ + C +L F
Sbjct: 270 VLKTFSLIFLNCTCLCVKCGFELWVF 295
>At1g72470.1 68414.m08380 exocyst subunit EXO70 family protein
contains Pfam domain PF03081: Exo70 exocyst complex
subunit;
Length = 633
Score = 27.9 bits (59), Expect = 7.1
Identities = 20/71 (28%), Positives = 33/71 (46%), Gaps = 11/71 (15%)
Frame = -3
Query: 566 HVSWATLRLNFSTSSYVTCCLDRGGALLRLNLIPDCHRLG-------DVLRLNWNLVE-- 414
H+SW R+ ++ Y+ C+ G++ + + RLG DV RLNW +E
Sbjct: 188 HLSWIARRM--VSAGYLRECIQVYGSVRKSAVDSSFRRLGIEKLSIGDVQRLNWEALEQK 245
Query: 413 --FWFRQSVVC 387
W R + +C
Sbjct: 246 IRRWIRAAKIC 256
>At3g24800.1 68416.m03112 PRT1 protein (PRT1) E3, N-end rule
ubiquitin ligase, contains two RING finger domain;
identical to PRT1 [Arabidopsis thaliana] GI:3319884
Length = 410
Score = 27.5 bits (58), Expect = 9.3
Identities = 14/39 (35%), Positives = 19/39 (48%), Gaps = 1/39 (2%)
Frame = +1
Query: 94 CASEGVYKDLNTTYHWMG-EEEVYGDMLKDCFNVQLSVP 207
C S GVY + Y +EE+ D+ KDC+ VP
Sbjct: 311 CDSCGVYPIIGDRYRCKDCKEEIGYDLCKDCYETPSKVP 349
>At3g23900.1 68416.m03003 RNA recognition motif (RRM)-containing
protein
Length = 987
Score = 27.5 bits (58), Expect = 9.3
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = +3
Query: 573 GRKNASSSHQERPS*GERKKYIKITPQDDERDAVNVKLR 689
G K +S + P R+K +K TP+DDE + V + R
Sbjct: 621 GEKKSSRAGSRSP---RRRKEVKSTPRDDEENKVKRRTR 656
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,360,191
Number of Sequences: 28952
Number of extensions: 261095
Number of successful extensions: 808
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 787
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 808
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1545769616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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