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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0060
         (733 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g18330.1 68416.m02332 F-box family protein contains Pfam PF00...    30   1.4  
At2g46150.1 68415.m05739 expressed protein  and genefinder             29   2.4  
At4g03570.1 68417.m00492 hypothetical protein                          29   3.2  
At5g16010.1 68418.m01872 3-oxo-5-alpha-steroid 4-dehydrogenase f...    28   5.6  

>At3g18330.1 68416.m02332 F-box family protein contains Pfam
           PF00646: F-box domain; contains TIGRFAM TIGR01640: F-box
           protein interaction domain
          Length = 376

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 12/48 (25%), Positives = 24/48 (50%)
 Frame = -3

Query: 371 LSRDISNTSSNAMEFRTEREIDIQNESYLKIVSNYLESQSGNTFICLI 228
           ++  I +T+  A+ +    E+D+  E +    SN+L  +    FIC +
Sbjct: 278 VTNKIDDTTEGAVSWTKVLELDLSRELHALFTSNFLVDEEKKVFICCV 325


>At2g46150.1 68415.m05739 expressed protein  and genefinder
          Length = 221

 Score = 29.5 bits (63), Expect = 2.4
 Identities = 12/37 (32%), Positives = 22/37 (59%)
 Frame = +1

Query: 295 SFCISISLSVLNSIALELVFEISRLSAPRVLLEGSLV 405
           S C++ +  +L +I L LVF + R+  P + + G +V
Sbjct: 39  SICVTATSLILTTIVLTLVFTVFRVKDPIIKMNGVMV 75


>At4g03570.1 68417.m00492 hypothetical protein 
          Length = 347

 Score = 29.1 bits (62), Expect = 3.2
 Identities = 13/37 (35%), Positives = 21/37 (56%)
 Frame = +1

Query: 544 YCYLKSRVLPTSLFVGHLPI*PFDCHIGICHIGSEPY 654
           YC  K +++ +  F G   + PFD +IGI  +G + Y
Sbjct: 40  YCLFKRQMIESKGFYGDPALKPFDHYIGIKPMGLQRY 76


>At5g16010.1 68418.m01872 3-oxo-5-alpha-steroid 4-dehydrogenase
           family protein / steroid 5-alpha-reductase family
           protein similar to steroid 5alpha-reductase - Rattus
           norvegicus, PIR:A34239 [SP|24008]; contains Pfam
           3-oxo-5-alpha-steroid 4-dehydrogenase domain PF02544
          Length = 268

 Score = 28.3 bits (60), Expect = 5.6
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = -3

Query: 239 ICLIYSFVQFVFYHYYRATPTIYSFDFS 156
           IC  Y F   VF+ ++  + TIYSF F+
Sbjct: 204 ICPHYLFEILVFWSFFLISQTIYSFSFA 231


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,455,727
Number of Sequences: 28952
Number of extensions: 241851
Number of successful extensions: 534
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 530
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 534
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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