Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Nnor0195
         (560 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri...   172   1e-43
At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast...   168   3e-42
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri...   167   6e-42
At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal, puta...   159   1e-39
At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal ident...   146   1e-35
At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative stron...    46   2e-05
At1g55540.1 68414.m06356 proline-rich family protein contains pr...    30   0.92 
At2g36700.1 68415.m04503 pectinesterase family protein contains ...    30   1.2  
At1g51540.1 68414.m05801 kelch repeat-containing protein contain...    28   4.9  
At2g47980.1 68415.m06004 expressed protein                             27   6.5  

>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
           putative similar to mitochondrial NAD-dependent malate
           dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
           thaliana]; contains InterPro entry IPR001236:
           Lactate/malate dehydrogenase
          Length = 341

 Score =  172 bits (419), Expect = 1e-43
 Identities = 86/165 (52%), Positives = 122/165 (73%), Gaps = 2/165 (1%)
 Frame = +1

Query: 70  MFSRALKPAALAVQNGA--KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLAL 243
           MF   +  +A  V+ G   + F++ S  + K         IGQPL+LL+K NPLV+ L+L
Sbjct: 1   MFRSMIVRSASPVKQGLLRRGFASESVPDRKVVILGAAGGIGQPLSLLMKLNPLVSSLSL 60

Query: 244 YDIAPVTPGVAADLSHVNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLF 423
           YDIA  TPGVAAD+ H+NT ++VSG+ G ++L  A++ AD+V+IPAGVPRKPGMTRDDLF
Sbjct: 61  YDIAN-TPGVAADVGHINTRSQVSGYMGDDDLGKALEGADLVIIPAGVPRKPGMTRDDLF 119

Query: 424 NTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIASEVLGKSG 558
           N NA IV++++++IA+  P+A+V +I+NPVNSTVPIA+E+  K+G
Sbjct: 120 NINAGIVKNLSIAIAKYCPQALVNMISNPVNSTVPIAAEIFKKAG 164


>At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast
           (MDH) identical to chloroplast NAD-malate dehydrogenase
           [Arabidopsis thaliana] GI:3256066; contains InterPro
           entry IPR001236: Lactate/malate dehydrogenase; contains
           Pfam profiles PF00056: lactate/malate dehydrogenase, NAD
           binding domain  and PF02866: lactate/malate
           dehydrogenase, alpha/beta C-terminal domain
          Length = 403

 Score =  168 bits (408), Expect = 3e-42
 Identities = 82/125 (65%), Positives = 100/125 (80%)
 Frame = +1

Query: 184 IGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHVNTPAKVSGHKGPEELSAAIKDAD 363
           IGQPL+LL+K +PLV+ L LYDIA V  GVAADLSH NTP++V    GP EL+  +KD +
Sbjct: 94  IGQPLSLLIKMSPLVSTLHLYDIANVK-GVAADLSHCNTPSQVRDFTGPSELADCLKDVN 152

Query: 364 VVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIASEV 543
           VVVIPAGVPRKPGMTRDDLFN NA+IV+ +  ++A+N P A + II+NPVNSTVPIA+EV
Sbjct: 153 VVVIPAGVPRKPGMTRDDLFNINANIVKTLVEAVAENCPNAFIHIISNPVNSTVPIAAEV 212

Query: 544 LGKSG 558
           L K G
Sbjct: 213 LKKKG 217


>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
           identical to mitochondrial NAD-dependent malate
           dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
           thaliana]; contains InterPro entry IPR001236:
           Lactate/malate dehydrogenase
          Length = 341

 Score =  167 bits (405), Expect = 6e-42
 Identities = 81/146 (55%), Positives = 110/146 (75%)
 Frame = +1

Query: 121 KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHVNT 300
           ++FS+ S    K         IGQPLALL+K NPLV+ L+LYDIA  TPGVAAD+ H+NT
Sbjct: 20  RSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIAN-TPGVAADVGHINT 78

Query: 301 PAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAP 480
            ++V G+ G + L+ A++ AD+V+IPAGVPRKPGMTRDDLFN NA IV+++  +IA+  P
Sbjct: 79  RSEVVGYMGDDNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLCTAIAKYCP 138

Query: 481 KAIVAIITNPVNSTVPIASEVLGKSG 558
            A++ +I+NPVNSTVPIA+E+  K+G
Sbjct: 139 HALINMISNPVNSTVPIAAEIFKKAG 164


>At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal,
           putative strong similarity to glyoxysomal malate
           dehydrogenase (EC 1.1.1.37) SP|P19446 {Citrullus
           lanatus}, SP|P46488 {Cucumis sativus}, [Medicago sativa]
           GI:2827078, SP|Q42972 {Oryza sativa}, SP|Q9ZP05
           {Arabidopsis thaliana}, SP|P37228 {Glycine max};
           contains InterPro entry IPR001236: Lactate/malate
           dehydrogenase
          Length = 354

 Score =  159 bits (386), Expect = 1e-39
 Identities = 81/136 (59%), Positives = 99/136 (72%)
 Frame = +1

Query: 151 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHVNTPAKVSGHKGP 330
           FK         IGQPLA+L+K NPLV+ L LYD+A   PGV AD+SH++T A V G  G 
Sbjct: 43  FKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVANA-PGVTADISHMDTSAVVRGFLGQ 101

Query: 331 EELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNP 510
            +L  A+   D+V+IPAGVPRKPGMTRDDLFN NA IVR ++ +IA+  PKAIV II+NP
Sbjct: 102 PQLEEALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLSEAIAKCCPKAIVNIISNP 161

Query: 511 VNSTVPIASEVLGKSG 558
           VNSTVPIA+EV  K+G
Sbjct: 162 VNSTVPIAAEVFKKAG 177


>At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal
           identical to SP|Q9ZP05; identical to cDNA microbody
           NAD-dependent malate dehydrogenase GI:3929650
          Length = 354

 Score =  146 bits (353), Expect = 1e-35
 Identities = 73/136 (53%), Positives = 94/136 (69%)
 Frame = +1

Query: 151 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHVNTPAKVSGHKGP 330
           FK         IGQ L+LL+K NPLV+ L LYD+    PGV AD+SH++T A V G  G 
Sbjct: 43  FKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVNA-PGVTADVSHMDTGAVVRGFLGA 101

Query: 331 EELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNP 510
           ++L  A+   D+V+IPAG+PRKPGMTRDDLF  NA IV+ +   +A+  P AIV +I+NP
Sbjct: 102 KQLEDALTGMDLVIIPAGIPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNP 161

Query: 511 VNSTVPIASEVLGKSG 558
           VNSTVPIA+EV  K+G
Sbjct: 162 VNSTVPIAAEVFKKAG 177


>At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative strong
           similarity to L-lactate dehydrogenase from Lycopersicon
           esculentum (GI:1620970, GI:1620972), Hordeum vulgare
           (SP|P22988, SP|P22989); contains InterPro entry
           IPR001236: Lactate/malate dehydrogenase
          Length = 353

 Score = 46.0 bits (104), Expect = 2e-05
 Identities = 33/125 (26%), Positives = 59/125 (47%), Gaps = 3/125 (2%)
 Frame = +1

Query: 184 IGQPLALLLKQNPLVTRLALYDIAP-VTPGVAADLSHVNT--PAKVSGHKGPEELSAAIK 354
           +G  +A  +    L   +AL D  P    G   DL H     P          E++A   
Sbjct: 51  VGMAIAQTILTQDLADEIALVDAKPDKLRGEMLDLQHAAAFLPRTKITASVDYEVTAG-- 108

Query: 355 DADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIA 534
            +D+ ++ AG  + PG +R +L   N ++ R I   +A+ +P +I+ I++NPV+    +A
Sbjct: 109 -SDLCIVTAGARQNPGESRLNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVDVLTYVA 167

Query: 535 SEVLG 549
            ++ G
Sbjct: 168 WKLSG 172


>At1g55540.1 68414.m06356 proline-rich family protein contains
           proline rich extensin domain, INTERPRO:IPR002965
          Length = 915

 Score = 30.3 bits (65), Expect = 0.92
 Identities = 13/54 (24%), Positives = 26/54 (48%)
 Frame = +1

Query: 376 PAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIAS 537
           P   P+KP      + +T +++      +  QN P  + + I+NP  +  P++S
Sbjct: 518 PVSEPKKPEAQSSSILSTQSTVDSVANATKTQNEPLPVKSEISNPGTTVTPVSS 571


>At2g36700.1 68415.m04503 pectinesterase family protein contains
           Pfam profile: PF01095 pectinesterase
          Length = 333

 Score = 29.9 bits (64), Expect = 1.2
 Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 1/47 (2%)
 Frame = -3

Query: 489 NSFGCILSNGQSNVTD-NGSISVE*VITGHSRFTGYSCWDDYNISIF 352
           N+ GCI +NG+  + D  G + V   ITG +R      W  Y   IF
Sbjct: 217 NTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIF 263


>At1g51540.1 68414.m05801 kelch repeat-containing protein contains
           Pfam profile PF01344: Kelch motif
          Length = 1036

 Score = 27.9 bits (59), Expect = 4.9
 Identities = 12/24 (50%), Positives = 15/24 (62%)
 Frame = -3

Query: 417 VITGHSRFTGYSCWDDYNISIFDG 346
           ++T HS FT    WD YN S+ DG
Sbjct: 314 LVTDHSSFTEKEAWDGYNKSL-DG 336


>At2g47980.1 68415.m06004 expressed protein
          Length = 1098

 Score = 27.5 bits (58), Expect = 6.5
 Identities = 13/33 (39%), Positives = 17/33 (51%)
 Frame = +1

Query: 220  PLVTRLALYDIAPVTPGVAADLSHVNTPAKVSG 318
            P  TRL++ DI  +   V   + HVNT    SG
Sbjct: 969  PFATRLSVSDIIDIKKDVQGRIVHVNTDEDPSG 1001


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.315    0.131    0.361 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,826,985
Number of Sequences: 28952
Number of extensions: 288873
Number of successful extensions: 745
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 740
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1072696904
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)