Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Nnor0175
(656 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 98 4e-21
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 91 8e-19
At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR... 32 0.29
At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR... 32 0.29
At5g07950.1 68418.m00922 expressed protein ferredoxin (2Fe-2S), ... 29 2.1
At1g29050.1 68414.m03556 expressed protein similar to hypothetic... 29 3.6
At2g24610.1 68415.m02940 cyclic nucleotide-regulated ion channel... 28 4.8
At3g52430.1 68416.m05766 phytoalexin-deficient 4 protein (PAD4) ... 28 6.3
At1g52150.2 68414.m05885 homeobox-leucine zipper family protein ... 28 6.3
At1g52150.1 68414.m05884 homeobox-leucine zipper family protein ... 28 6.3
At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative ... 28 6.3
At5g54650.2 68418.m06805 formin homology 2 domain-containing pro... 27 8.3
At5g54650.1 68418.m06804 formin homology 2 domain-containing pro... 27 8.3
At5g10530.1 68418.m01219 lectin protein kinase, putative similar... 27 8.3
At4g26300.1 68417.m03783 arginyl-tRNA synthetase, putative / arg... 27 8.3
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g... 27 8.3
At4g20730.1 68417.m03013 filament protein-related similar to Cyt... 27 8.3
At4g02560.1 68417.m00350 homeobox protein LUMINIDEPENDENS (LD) i... 27 8.3
At3g58610.1 68416.m06532 ketol-acid reductoisomerase identical t... 27 8.3
At3g10100.1 68416.m01210 filament protein-related similar to YEA... 27 8.3
At2g17010.1 68415.m01961 mechanosensitive ion channel domain-con... 27 8.3
At1g27430.1 68414.m03343 GYF domain-containing protein contains ... 27 8.3
At1g24300.1 68414.m03066 GYF domain-containing protein contains ... 27 8.3
At1g19130.1 68414.m02379 expressed protein 27 8.3
>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 98.3 bits (234), Expect = 4e-21
Identities = 66/194 (34%), Positives = 98/194 (50%), Gaps = 3/194 (1%)
Frame = +1
Query: 46 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 225
MS K EKP L G RIKTRKR+ DP F DA+VQ GDL+ K ++S S L
Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIES--SDL 57
Query: 226 DYRRYGEVIFDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMR 405
++ RYG++ F+V+ GG PG S +GE + + + + K++R
Sbjct: 58 NFTRYGDIFFEVIFIGGRTQPGTVKSDEGE--RHTYSVIDCEPKREAILPSVVYIQKILR 115
Query: 406 RYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARMTALWIG---NGCVPPSVLLVLVNEHL 576
R +L K E ++ L L+ F+ +R KLA TAL +G P +V L+ ++L
Sbjct: 116 RKPFLIKNLENVTRRFLQSLELFEENERKKLAIFTALAFSQKLSGLPPETVFQPLLKDNL 175
Query: 577 LKDNLALDFVLEVF 618
+ + L FV + F
Sbjct: 176 VAKGIVLSFVTDFF 189
>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|P47823
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Oryctolagus
cuniculus}; contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 90.6 bits (215), Expect = 8e-19
Identities = 66/194 (34%), Positives = 96/194 (49%), Gaps = 3/194 (1%)
Frame = +1
Query: 46 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 225
MS K EKP L G RIKTRKR+ DP F DALVQ GDL+ + L+S S L
Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDALVQIYLDNAGDLELVARSLES--SDL 57
Query: 226 DYRRYGEVIFDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMR 405
++ RYG++ F+V+ GG G S +GE + I + + K++R
Sbjct: 58 NFSRYGDIFFEVVFIGGRTQTGSVKSDEGE--RHPYSIIDCEPKREAILPSVVYIQKILR 115
Query: 406 RYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARMTALWIG---NGCVPPSVLLVLVNEHL 576
R +L K E ++ L L+ F+ +R KLA TAL +G +V L+ + L
Sbjct: 116 RKAFLIKNLENVTRRFLQSLELFEENERKKLAIFTALAFSQKLSGLPAETVFQPLLKDTL 175
Query: 577 LKDNLALDFVLEVF 618
+ + L+FV + F
Sbjct: 176 VAKGIVLNFVTDFF 189
>At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 303
Score = 32.3 bits (70), Expect = 0.29
Identities = 22/70 (31%), Positives = 34/70 (48%), Gaps = 1/70 (1%)
Frame = -2
Query: 622 SRTLPARSPAPGCPSADVRSPGPAARMEARIRYRSTVQSCAPA*CAVRDRSLS-SRPEPF 446
SR+ A+S + RSPGP ++ + RS +S +P ++ S S S+P P
Sbjct: 233 SRSPKAKSSRRSPAKSTSRSPGPRSKSRSPSPRRSRSRSRSPLPSVQKEGSKSPSKPSPA 292
Query: 445 SFPLRTFFPS 416
P+ T PS
Sbjct: 293 KSPIHTRSPS 302
>At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 303
Score = 32.3 bits (70), Expect = 0.29
Identities = 22/70 (31%), Positives = 34/70 (48%), Gaps = 1/70 (1%)
Frame = -2
Query: 622 SRTLPARSPAPGCPSADVRSPGPAARMEARIRYRSTVQSCAPA*CAVRDRSLS-SRPEPF 446
SR+ A+S + RSPGP ++ + RS +S +P ++ S S S+P P
Sbjct: 233 SRSPKAKSSRRSPAKSTSRSPGPRSKSRSPSPRRSRSRSRSPLPSVQKEGSKSPSKPSPA 292
Query: 445 SFPLRTFFPS 416
P+ T PS
Sbjct: 293 KSPIHTRSPS 302
>At5g07950.1 68418.m00922 expressed protein ferredoxin (2Fe-2S),
Arabidopsis thaliana, PIR:B71412
Length = 303
Score = 29.5 bits (63), Expect = 2.1
Identities = 17/45 (37%), Positives = 28/45 (62%)
Frame = -2
Query: 637 APA**SRTLPARSPAPGCPSADVRSPGPAARMEARIRYRSTVQSC 503
+PA SR+L + SP+PG S+ +R PAA+ R ++ S + +C
Sbjct: 13 SPASSSRSLASSSPSPGSFSSSMRLWRPAAQRNLRNQW-SNLSNC 56
>At1g29050.1 68414.m03556 expressed protein similar to hypothetical
protein GB:AAB67625 GI:2342727 from [Arabidopsis
thaliana]
Length = 380
Score = 28.7 bits (61), Expect = 3.6
Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 3/60 (5%)
Frame = -3
Query: 570 FVHQDQQHGWRHASVTDPQCSHARQLDALF-GIEAFQ--VDQNLFHFLFEHFFQVLVPTH 400
+ H+ Q GW + +LDA + G+ + VDQN+ FFQ + PTH
Sbjct: 224 WTHKGQSQGWDYIRDGSSLVRDMNRLDAFYKGLSTWARWVDQNVDTAKTRVFFQGISPTH 283
>At2g24610.1 68415.m02940 cyclic nucleotide-regulated ion channel,
putative (CNGC14) similar to cyclic nucleotide and
calmodulin-regulated ion channel (GI:4581205)
[Arabidopsis thaliana]
Length = 726
Score = 28.3 bits (60), Expect = 4.8
Identities = 10/40 (25%), Positives = 22/40 (55%)
Frame = -3
Query: 567 VHQDQQHGWRHASVTDPQCSHARQLDALFGIEAFQVDQNL 448
+H+D ++ W++ +V C + + FGI A + +N+
Sbjct: 302 LHRDDRNNWQNTTVVFSNCDPSNNIQFTFGIFADALTKNV 341
>At3g52430.1 68416.m05766 phytoalexin-deficient 4 protein (PAD4)
identical to phytoalexin-deficient 4 protein
[Arabidopsis thaliana] GI:6457331; contains Pfam profile
PF01764: Lipase
Length = 541
Score = 27.9 bits (59), Expect = 6.3
Identities = 21/76 (27%), Positives = 32/76 (42%)
Frame = +1
Query: 319 PKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQRIKL 498
P T +F + + + +KL + K E + E + K LV + G L
Sbjct: 66 PVTGDVLFPGLSSDEPLPMVDAAILKLFLQLKIKEGLELELLGKKLVVITGHSTGGA--L 123
Query: 499 ARMTALWIGNGCVPPS 546
A TALW+ + PPS
Sbjct: 124 AAFTALWLLSQSSPPS 139
>At1g52150.2 68414.m05885 homeobox-leucine zipper family protein /
lipid-binding START domain-containing protein similar to
to HD-zip transcription factor (athb-8) (GI:7270235)
[Arabidopsis thaliana]; contains Pfam profiles PF01852:
START domain, PF00046: Homeobox domain
Length = 837
Score = 27.9 bits (59), Expect = 6.3
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +1
Query: 454 LVYLKGFDPEQRIKLARMTALWIGNGCVPPSVLLVLVNEH 573
L + GF P + L ++ + N VPP++LL + EH
Sbjct: 440 LTFANGFAPVSNVVLCAKASMLLQN--VPPAILLRFLREH 477
>At1g52150.1 68414.m05884 homeobox-leucine zipper family protein /
lipid-binding START domain-containing protein similar to
to HD-zip transcription factor (athb-8) (GI:7270235)
[Arabidopsis thaliana]; contains Pfam profiles PF01852:
START domain, PF00046: Homeobox domain
Length = 836
Score = 27.9 bits (59), Expect = 6.3
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +1
Query: 454 LVYLKGFDPEQRIKLARMTALWIGNGCVPPSVLLVLVNEH 573
L + GF P + L ++ + N VPP++LL + EH
Sbjct: 440 LTFANGFAPVSNVVLCAKASMLLQN--VPPAILLRFLREH 477
>At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative
similar to GTL1 [Arabidopsis thaliana] GI:2664198
Length = 669
Score = 27.9 bits (59), Expect = 6.3
Identities = 15/54 (27%), Positives = 25/54 (46%)
Frame = -1
Query: 401 ISFTNTCSKFRMVSISSLAEKMQVLVLGDSPSIDTEPPGSSRPPAMSTSNMTSP 240
+S S R +I SL +K+ + PS+ ++PP +PP T + P
Sbjct: 321 MSQERAASASRDAAIISLIQKITGHTIQLPPSLSSQPPPPYQPPPAVTKRVAEP 374
>At5g54650.2 68418.m06805 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 27.5 bits (58), Expect = 8.3
Identities = 17/40 (42%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
Frame = -1
Query: 320 GDSPSID-TEPPGSSRPPAMSTSNMTSP*RR*SSFDPAES 204
G SPS + PP SR P + SP R SSF P+ S
Sbjct: 143 GSSPSPSPSRPPKRSRGPPRPPTRPKSPPPRKSSFPPSRS 182
>At5g54650.1 68418.m06804 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 27.5 bits (58), Expect = 8.3
Identities = 17/40 (42%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
Frame = -1
Query: 320 GDSPSID-TEPPGSSRPPAMSTSNMTSP*RR*SSFDPAES 204
G SPS + PP SR P + SP R SSF P+ S
Sbjct: 143 GSSPSPSPSRPPKRSRGPPRPPTRPKSPPPRKSSFPPSRS 182
>At5g10530.1 68418.m01219 lectin protein kinase, putative similar to
receptor lectin kinase 3 [Arabidopsis thaliana]
gi|4100060|gb|AAD00733; contains protein kinase domain,
Pfam:PF00069; contains legume lectins alpha and beta
domains, Pfam:PF00138 and Pfam:PF00139
Length = 651
Score = 27.5 bits (58), Expect = 8.3
Identities = 18/62 (29%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = +1
Query: 97 RKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL-DYRRYGEVIFDVLIAG 273
+++ +K+K + ++ + LER G YK L SA + D R+ GE F + G
Sbjct: 293 KRKQQKKKAEETENLTSINEDLERGAGPRKFTYKDLASAANNFADDRKLGEGGFGAVYRG 352
Query: 274 GL 279
L
Sbjct: 353 YL 354
>At4g26300.1 68417.m03783 arginyl-tRNA synthetase, putative /
arginine--tRNA ligase, putative similar to SP|P37880
Arginyl-tRNA synthetase (EC 6.1.1.19) (Arginine--tRNA
ligase) (ArgRS) {Cricetulus longicaudatus}; contains
Pfam profiles PF00750: arginyl-tRNA synthetase, PF03485:
arginyl-tRNA synthetase N-terminal domain
Length = 642
Score = 27.5 bits (58), Expect = 8.3
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +1
Query: 382 QVFVKLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARM 507
QVF K + L++ F+E+ ++ +V L+G DP R A++
Sbjct: 260 QVFYKASKHKFDLDEAFKEKAQQAVVRLQGGDPVYRKAWAKI 301
>At4g25000.1 68417.m03587 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative similar to
alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
GI:21670851; contains Pfam profile PF00128: Alpha
amylase, catalytic domain
Length = 423
Score = 27.5 bits (58), Expect = 8.3
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +1
Query: 106 DEKEKYDPNGFRDALVQGLERAGGDLDAAYKY 201
D K YD N R L Q +E AGG + A+ +
Sbjct: 238 DGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDF 269
>At4g20730.1 68417.m03013 filament protein-related similar to
Cytadherence high molecular weight protein 2 (SP:P47460)
[Mycoplasma genitalium]; similar to YEAST NUF1
protein (Spindle poly body spacer protein SPC110)
(SP:P32380) {Saccharomyces cerevisiae}; also
SP|Q9UKX2|MYH2_HUMAN Myosin heavy chain, skeletal
muscle, SP|P31732|OV71_ONCVO Muscle cell intermediate
filament protein SP|P12882|MYH1_HUMAN Myosin heavy
chain, skeletal muscle,. SP|Q17107|AV71_ACAVI Muscle
cell intermediate filament protein
Length = 800
Score = 27.5 bits (58), Expect = 8.3
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +3
Query: 393 ETDASVQVLGKNVRRGNEKGSGLLERLRSRTAHQAG 500
+ D +V LG + R EKG G+ + + R+A +AG
Sbjct: 455 DKDETVAELGISRRSREEKGKGVATQSKKRSASEAG 490
>At4g02560.1 68417.m00350 homeobox protein LUMINIDEPENDENS (LD)
identical to Homeobox protein LUMINIDEPENDENS
(Swiss-Prot:Q38796) [Arabidopsis thaliana]
Length = 953
Score = 27.5 bits (58), Expect = 8.3
Identities = 21/61 (34%), Positives = 30/61 (49%), Gaps = 2/61 (3%)
Frame = +2
Query: 320 PRPTPASSPPMRIWTPCEISNRYS*N*CVGTSTWKKCSK-RK*KRFW-ST*KASIPNSAS 493
P PTP+++P M W I N +S VGT+ + ++ R W T + SIP A
Sbjct: 753 PSPTPSTNPGMSGWGQEGIRNPFSRQNQVGTAVARSGTQLRVGSMQWHQTNEQSIPRHAP 812
Query: 494 S 496
S
Sbjct: 813 S 813
>At3g58610.1 68416.m06532 ketol-acid reductoisomerase identical to
ketol-acid reductoisomerase, chloroplast precursor (EC
1.1.1.86) (Acetohydroxy-acid reductoisomerase)
(Alpha-keto-beta-hydroxylacil reductoisomerase)
(Swiss-Prot:Q05758) [Arabidopsis thaliana]
Length = 591
Score = 27.5 bits (58), Expect = 8.3
Identities = 16/50 (32%), Positives = 23/50 (46%)
Frame = +1
Query: 469 GFDPEQRIKLARMTALWIGNGCVPPSVLLVLVNEHLLKDNLALDFVLEVF 618
GF L +TA GNG S+ +V+ +K ++LDF VF
Sbjct: 38 GFLSSSSKSLRSLTATVAGNGATGSSLAARMVSSSAVKAPVSLDFETSVF 87
>At3g10100.1 68416.m01210 filament protein-related similar to YEAST
NUF1 protein (Spindle poly body spacer protein SPC110)
(SP:P32380) {Saccharomyces cerevisiae}; similar to
Myosin heavy chain, smooth muscle isoform (SMMHC)
(SP:P35749) {Homo sapiens}
Length = 1004
Score = 27.5 bits (58), Expect = 8.3
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +3
Query: 408 VQVLGKNVRRGNEKGSGLLERLRSRTAHQAGAHDCTVDR 524
V LG + R EKG GL + + R+A +AG + +
Sbjct: 459 VAELGASRRSREEKGKGLASQSKKRSASEAGLDEAAAPK 497
>At2g17010.1 68415.m01961 mechanosensitive ion channel
domain-containing protein / MS ion channel
domain-containing protein contains Pfam profile PF00924:
Mechanosensitive ion channel
Length = 779
Score = 27.5 bits (58), Expect = 8.3
Identities = 16/60 (26%), Positives = 26/60 (43%)
Frame = +1
Query: 208 SAGSKLDYRRYGEVIFDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQV 387
+A + D R V F+V AGG + G S S +++ N+D ++ E V
Sbjct: 165 TARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQPQLQEEEVV 224
>At1g27430.1 68414.m03343 GYF domain-containing protein contains
Pfam profile: PF02213 GYF domain
Length = 1531
Score = 27.5 bits (58), Expect = 8.3
Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = -3
Query: 630 LDDREHFQHEVQRQVVLQQMFVHQ-DQQHGWRHASVTDPQCSHARQL 493
+ ++ Q + Q+++ LQQ HQ +Q+H + + Q SHARQL
Sbjct: 796 MQQQQKIQLQQQQKIQLQQ---HQLEQEHQLHQKLLQEQQQSHARQL 839
>At1g24300.1 68414.m03066 GYF domain-containing protein contains
Pfam profile: PF02213 GYF domain
Length = 1417
Score = 27.5 bits (58), Expect = 8.3
Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = -3
Query: 630 LDDREHFQHEVQRQVVLQQMFVHQ-DQQHGWRHASVTDPQCSHARQL 493
L ++ Q + Q+++ LQQ HQ +Q+H + + Q SHARQL
Sbjct: 727 LQQQQKIQLQQQQKMQLQQ---HQLEQEHQLHQKLLQEQQQSHARQL 770
>At1g19130.1 68414.m02379 expressed protein
Length = 187
Score = 27.5 bits (58), Expect = 8.3
Identities = 15/51 (29%), Positives = 24/51 (47%)
Frame = +1
Query: 280 LLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMF 432
LLP GSVS P T F E + +R+ ++ ++L++MF
Sbjct: 59 LLPSGSVSRLHRIPMAETWHFYLGEPLTVIRSLSTRSLQTSGLVRFLQRMF 109
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,274,830
Number of Sequences: 28952
Number of extensions: 354626
Number of successful extensions: 1410
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1289
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1402
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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