Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021141
(733 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast... 125 3e-29
At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal, puta... 120 8e-28
At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri... 118 3e-27
At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal ident... 114 7e-26
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri... 113 2e-25
At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative stron... 44 1e-04
At1g55540.1 68414.m06356 proline-rich family protein contains pr... 31 0.60
At2g36700.1 68415.m04503 pectinesterase family protein contains ... 30 1.8
At4g31850.1 68417.m04525 pentatricopeptide (PPR) repeat-containi... 29 2.4
At4g21030.1 68417.m03042 Dof-type zinc finger domain-containing ... 29 4.2
At2g04860.1 68415.m00501 pentatricopeptide (PPR) repeat-containi... 28 5.6
At1g68430.1 68414.m07817 expressed protein 28 7.3
At1g51540.1 68414.m05801 kelch repeat-containing protein contain... 28 7.3
At1g77800.1 68414.m09059 PHD finger family protein contains Pfam... 27 9.7
>At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast
(MDH) identical to chloroplast NAD-malate dehydrogenase
[Arabidopsis thaliana] GI:3256066; contains InterPro
entry IPR001236: Lactate/malate dehydrogenase; contains
Pfam profiles PF00056: lactate/malate dehydrogenase, NAD
binding domain and PF02866: lactate/malate
dehydrogenase, alpha/beta C-terminal domain
Length = 403
Score = 125 bits (301), Expect = 3e-29
Identities = 61/118 (51%), Positives = 82/118 (69%), Gaps = 1/118 (0%)
Frame = +3
Query: 252 SDLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIA 431
+DLSH NTP++V GP EL+ +KD +VVVIPAGVPRKPGMTRDDLFN NA+IV+ +
Sbjct: 124 ADLSHCNTPSQVRDFTGPSELADCLKDVNVVVIPAGVPRKPGMTRDDLFNINANIVKTLV 183
Query: 432 LSIAQNAPKAIVAIITNPVNSTVPIALKCSRRREC-TTRTACSASPLWTYRAATFVGE 602
++A+N P A + II+NPVNSTVPIA + +++ + + L RA TFV +
Sbjct: 184 EAVAENCPNAFIHIISNPVNSTVPIAAEVLKKKGVYDPKKLFGVTTLDVVRANTFVSQ 241
Score = 69.7 bits (163), Expect = 2e-12
Identities = 35/70 (50%), Positives = 52/70 (74%), Gaps = 2/70 (2%)
Frame = +2
Query: 509 SEVLKKAGVYDPNRVLGVTTLDVSRCH--LRRRDQWRGPNSVAVPVIGGHSGITIIPILS 682
+EVLKK GVYDP ++ GVTTLDV R + + ++ + + V VPVIGGH+GITI+P+LS
Sbjct: 210 AEVLKKKGVYDPKKLFGVTTLDVVRANTFVSQKKNLKLID-VDVPVIGGHAGITILPLLS 268
Query: 683 QCQPALKLSD 712
+ +P++ +D
Sbjct: 269 KTKPSVNFTD 278
Score = 41.5 bits (93), Expect = 6e-04
Identities = 22/32 (68%), Positives = 26/32 (81%)
Frame = +1
Query: 160 IGQPLALLLKQNPLVTRLALYDIAPVTPGVAA 255
IGQPL+LL+K +PLV+ L LYDIA V GVAA
Sbjct: 94 IGQPLSLLIKMSPLVSTLHLYDIANV-KGVAA 124
>At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal,
putative strong similarity to glyoxysomal malate
dehydrogenase (EC 1.1.1.37) SP|P19446 {Citrullus
lanatus}, SP|P46488 {Cucumis sativus}, [Medicago sativa]
GI:2827078, SP|Q42972 {Oryza sativa}, SP|Q9ZP05
{Arabidopsis thaliana}, SP|P37228 {Glycine max};
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 354
Score = 120 bits (290), Expect = 8e-28
Identities = 64/127 (50%), Positives = 83/127 (65%), Gaps = 1/127 (0%)
Frame = +3
Query: 240 PRRRSDLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIV 419
P +D+SHM+T A V G G +L A+ D+V+IPAGVPRKPGMTRDDLFN NA IV
Sbjct: 80 PGVTADISHMDTSAVVRGFLGQPQLEEALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIV 139
Query: 420 RDIALSIAQNAPKAIVAIITNPVNSTVPIALKCSRRR-ECTTRTACSASPLWTYRAATFV 596
R ++ +IA+ PKAIV II+NPVNSTVPIA + ++ + + L RA TFV
Sbjct: 140 RTLSEAIAKCCPKAIVNIISNPVNSTVPIAAEVFKKAGTFDPKKLMGVTMLDVVRANTFV 199
Query: 597 GEINGVD 617
E+ +D
Sbjct: 200 AEVMSLD 206
Score = 68.5 bits (160), Expect = 4e-12
Identities = 31/70 (44%), Positives = 46/70 (65%), Gaps = 1/70 (1%)
Frame = +2
Query: 509 SEVLKKAGVYDPNRVLGVTTLDVSRCHLRRRDQWR-GPNSVAVPVIGGHSGITIIPILSQ 685
+EV KKAG +DP +++GVT LDV R + + P V VPV+GGH+G+TI+P+LSQ
Sbjct: 170 AEVFKKAGTFDPKKLMGVTMLDVVRANTFVAEVMSLDPREVEVPVVGGHAGVTILPLLSQ 229
Query: 686 CQPALKLSDR 715
+P + +
Sbjct: 230 VKPPCSFTQK 239
Score = 43.6 bits (98), Expect = 1e-04
Identities = 23/43 (53%), Positives = 27/43 (62%)
Frame = +1
Query: 127 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAA 255
FK IGQPLA+L+K NPLV+ L LYD+A PGV A
Sbjct: 43 FKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVAN-APGVTA 84
>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
putative similar to mitochondrial NAD-dependent malate
dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 118 bits (285), Expect = 3e-27
Identities = 52/96 (54%), Positives = 78/96 (81%)
Frame = +3
Query: 240 PRRRSDLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIV 419
P +D+ H+NT ++VSG+ G ++L A++ AD+V+IPAGVPRKPGMTRDDLFN NA IV
Sbjct: 67 PGVAADVGHINTRSQVSGYMGDDDLGKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIV 126
Query: 420 RDIALSIAQNAPKAIVAIITNPVNSTVPIALKCSRR 527
++++++IA+ P+A+V +I+NPVNSTVPIA + ++
Sbjct: 127 KNLSIAIAKYCPQALVNMISNPVNSTVPIAAEIFKK 162
Score = 66.1 bits (154), Expect = 2e-11
Identities = 35/73 (47%), Positives = 46/73 (63%), Gaps = 5/73 (6%)
Frame = +2
Query: 509 SEVLKKAGVYDPNRVLGVTTLDVSRCHLRRRDQWRGPNSVAV-----PVIGGHSGITIIP 673
+E+ KKAG YD ++ GVTTLDV +R R + G + V V PV+GGH+GITI+P
Sbjct: 157 AEIFKKAGTYDEKKLFGVTTLDV----VRARTFYAGKSDVNVAEVNVPVVGGHAGITILP 212
Query: 674 ILSQCQPALKLSD 712
+ SQ P LSD
Sbjct: 213 LFSQASPQANLSD 225
Score = 50.8 bits (116), Expect = 9e-07
Identities = 32/72 (44%), Positives = 43/72 (59%), Gaps = 2/72 (2%)
Frame = +1
Query: 46 MFSRALKPAALAVQNGA--KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLAL 219
MF + +A V+ G + F++ S + K IGQPL+LL+K NPLV+ L+L
Sbjct: 1 MFRSMIVRSASPVKQGLLRRGFASESVPDRKVVILGAAGGIGQPLSLLMKLNPLVSSLSL 60
Query: 220 YDIAPVTPGVAA 255
YDIA TPGVAA
Sbjct: 61 YDIAN-TPGVAA 71
>At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal
identical to SP|Q9ZP05; identical to cDNA microbody
NAD-dependent malate dehydrogenase GI:3929650
Length = 354
Score = 114 bits (274), Expect = 7e-26
Identities = 59/127 (46%), Positives = 81/127 (63%), Gaps = 1/127 (0%)
Frame = +3
Query: 240 PRRRSDLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIV 419
P +D+SHM+T A V G G ++L A+ D+V+IPAG+PRKPGMTRDDLF NA IV
Sbjct: 80 PGVTADVSHMDTGAVVRGFLGAKQLEDALTGMDLVIIPAGIPRKPGMTRDDLFKINAGIV 139
Query: 420 RDIALSIAQNAPKAIVAIITNPVNSTVPIALKCSRRR-ECTTRTACSASPLWTYRAATFV 596
+ + +A+ P AIV +I+NPVNSTVPIA + ++ + + L RA TFV
Sbjct: 140 KTLCEGVAKCCPNAIVNLISNPVNSTVPIAAEVFKKAGTYDPKKLLGVTTLDVARANTFV 199
Query: 597 GEINGVD 617
E+ G+D
Sbjct: 200 AEVLGLD 206
Score = 72.9 bits (171), Expect = 2e-13
Identities = 34/63 (53%), Positives = 46/63 (73%), Gaps = 1/63 (1%)
Frame = +2
Query: 509 SEVLKKAGVYDPNRVLGVTTLDVSRCHLRRRDQWR-GPNSVAVPVIGGHSGITIIPILSQ 685
+EV KKAG YDP ++LGVTTLDV+R + + P V VPV+GGH+G+TI+P+LSQ
Sbjct: 170 AEVFKKAGTYDPKKLLGVTTLDVARANTFVAEVLGLDPREVDVPVVGGHAGVTILPLLSQ 229
Query: 686 CQP 694
+P
Sbjct: 230 VKP 232
Score = 38.7 bits (86), Expect = 0.004
Identities = 21/43 (48%), Positives = 25/43 (58%)
Frame = +1
Query: 127 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAA 255
FK IGQ L+LL+K NPLV+ L LYD+ PGV A
Sbjct: 43 FKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVN-APGVTA 84
>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
identical to mitochondrial NAD-dependent malate
dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 113 bits (271), Expect = 2e-25
Identities = 50/96 (52%), Positives = 74/96 (77%)
Frame = +3
Query: 240 PRRRSDLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIV 419
P +D+ H+NT ++V G+ G + L+ A++ AD+V+IPAGVPRKPGMTRDDLFN NA IV
Sbjct: 67 PGVAADVGHINTRSEVVGYMGDDNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIV 126
Query: 420 RDIALSIAQNAPKAIVAIITNPVNSTVPIALKCSRR 527
+++ +IA+ P A++ +I+NPVNSTVPIA + ++
Sbjct: 127 KNLCTAIAKYCPHALINMISNPVNSTVPIAAEIFKK 162
Score = 66.5 bits (155), Expect = 2e-11
Identities = 33/68 (48%), Positives = 44/68 (64%), Gaps = 1/68 (1%)
Frame = +2
Query: 509 SEVLKKAGVYDPNRVLGVTTLDVSRCHLRRRDQWRGPNS-VAVPVIGGHSGITIIPILSQ 685
+E+ KKAG+YD ++ GVTTLDV R + P + V VPVIGGH+G+TI+P+ SQ
Sbjct: 157 AEIFKKAGMYDEKKLFGVTTLDVVRARTFYAGKANVPVAEVNVPVIGGHAGVTILPLFSQ 216
Query: 686 CQPALKLS 709
P LS
Sbjct: 217 ATPQANLS 224
Score = 50.8 bits (116), Expect = 9e-07
Identities = 29/53 (54%), Positives = 35/53 (66%)
Frame = +1
Query: 97 KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAA 255
++FS+ S K IGQPLALL+K NPLV+ L+LYDIA TPGVAA
Sbjct: 20 RSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIAN-TPGVAA 71
>At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative strong
similarity to L-lactate dehydrogenase from Lycopersicon
esculentum (GI:1620970, GI:1620972), Hordeum vulgare
(SP|P22988, SP|P22989); contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 353
Score = 44.0 bits (99), Expect = 1e-04
Identities = 20/63 (31%), Positives = 38/63 (60%)
Frame = +3
Query: 333 ADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIAL 512
+D+ ++ AG + PG +R +L N ++ R I +A+ +P +I+ I++NPV+ +A
Sbjct: 109 SDLCIVTAGARQNPGESRLNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVDVLTYVAW 168
Query: 513 KCS 521
K S
Sbjct: 169 KLS 171
>At1g55540.1 68414.m06356 proline-rich family protein contains
proline rich extensin domain, INTERPRO:IPR002965
Length = 915
Score = 31.5 bits (68), Expect = 0.60
Identities = 25/114 (21%), Positives = 43/114 (37%), Gaps = 1/114 (0%)
Frame = +3
Query: 171 FGPSTE-AESSGDQAGFIRHSACDPRRRSDLSHMNTPAKVSGHKGPEELSAAIKDADVVV 347
F PS ++ S DQA R S S +TP + A +
Sbjct: 457 FPPSAPTSQVSSDQASATSSLTDSSRLFSSTSLSSTPPITPPDAFQSPQVSTPSSAVPIT 516
Query: 348 IPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIA 509
P P+KP + +T +++ + QN P + + I+NP + P++
Sbjct: 517 EPVSEPKKPEAQSSSILSTQSTVDSVANATKTQNEPLPVKSEISNPGTTVTPVS 570
>At2g36700.1 68415.m04503 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 333
Score = 29.9 bits (64), Expect = 1.8
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 1/47 (2%)
Frame = -3
Query: 464 NSFGCILSNGQSNVTD-NGSISVE*VITGHSRFTGYSCWDDYNISIF 327
N+ GCI +NG+ + D G + V ITG +R W Y IF
Sbjct: 217 NTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIF 263
>At4g31850.1 68417.m04525 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 1112
Score = 29.5 bits (63), Expect = 2.4
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = -3
Query: 290 ADLGWGVHVGKVAATPGVTGAMSYKASLVTRGFC 189
A+L W +G + A G+ A+S+ LV G C
Sbjct: 712 ANLFWEDLIGSILAEAGIDNAVSFSERLVANGIC 745
>At4g21030.1 68417.m03042 Dof-type zinc finger domain-containing
protein prolamin box binding factor, Zea mays,
PID:g2393775
Length = 194
Score = 28.7 bits (61), Expect = 4.2
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +3
Query: 6 SNIHVYTR*TNNENVLPRPKTCRPC 80
+N++V+T N NV+P P+ C C
Sbjct: 2 NNLNVFTNEDNEMNVMPPPRVCPRC 26
>At2g04860.1 68415.m00501 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 582
Score = 28.3 bits (60), Expect = 5.6
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = -1
Query: 622 VRSTPLISPTKVAARYVQSGDAEHAVRVVHSRLL 521
++ TPLIS V + VQSG A A V H +L
Sbjct: 298 LQETPLISWNSVISGCVQSGRASTAFEVFHQMML 331
>At1g68430.1 68414.m07817 expressed protein
Length = 146
Score = 27.9 bits (59), Expect = 7.3
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = +1
Query: 244 GVAATFPT*TPQPRSAATRDLRSYQQPSKMLM 339
GVAA PT +P PR++ LR + KML+
Sbjct: 15 GVAAQSPTRSPSPRTSPLVQLRRKKTTLKMLL 46
>At1g51540.1 68414.m05801 kelch repeat-containing protein contains
Pfam profile PF01344: Kelch motif
Length = 1036
Score = 27.9 bits (59), Expect = 7.3
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -3
Query: 392 VITGHSRFTGYSCWDDYNISIFDG 321
++T HS FT WD YN S+ DG
Sbjct: 314 LVTDHSSFTEKEAWDGYNKSL-DG 336
>At1g77800.1 68414.m09059 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 1423
Score = 27.5 bits (58), Expect = 9.7
Identities = 18/86 (20%), Positives = 32/86 (37%)
Frame = +3
Query: 69 CRPCCTKWCKELFHHITEEL*SGGRWCRRWYRPAFGPSTEAESSGDQAGFIRHSACDPRR 248
C CC C+ FH I G W + ++ + SG +A +
Sbjct: 411 CIRCCNGTCRTSFHPICAR--EAGNRLEVWGKHGCDTHSDIQESGKSVEGGESNAAE--S 466
Query: 249 RSDLSHMNTPAKVSGHKGPEELSAAI 326
RS + H+ + + GH +E+ +
Sbjct: 467 RSPICHLPSESVGEGHLSNDEMGVDV 492
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,333,546
Number of Sequences: 28952
Number of extensions: 374391
Number of successful extensions: 987
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 950
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 982
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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