Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021116
(799 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g17410.2 68418.m02043 tubulin family protein similar to spind... 32 0.51
At5g17410.1 68418.m02042 tubulin family protein similar to spind... 32 0.51
At3g53760.1 68416.m05939 tubulin family protein similar to SP|Q9... 31 0.67
At3g54460.1 68416.m06025 SNF2 domain-containing protein / helica... 31 0.89
At1g80260.1 68414.m09396 tubulin family protein 31 0.89
At1g20570.1 68414.m02565 tubulin family protein 31 0.89
At5g49110.1 68418.m06079 expressed protein ; expression support... 30 1.5
At5g15540.1 68418.m01819 expressed protein low similarity to DNA... 29 3.6
At3g47770.1 68416.m05204 ABC transporter family protein AbcA, Di... 29 4.7
At5g15070.1 68418.m01766 expressed protein 28 6.2
At5g48060.1 68418.m05938 C2 domain-containing protein contains I... 28 8.3
At3g43610.1 68416.m04640 tubulin family protein 28 8.3
At2g13680.1 68415.m01508 glycosyl transferase family 48 protein ... 28 8.3
At1g71020.1 68414.m08197 armadillo/beta-catenin repeat family pr... 28 8.3
>At5g17410.2 68418.m02043 tubulin family protein similar to spindle
pole body protein [Homo
sapiens][GI:2801701][PMID:9566967], gamma-tubulin ring
protein Dgrip84 [Drosophila
melanogaster][GI:4689225][PMID: 10037793]
Length = 679
Score = 31.9 bits (69), Expect = 0.51
Identities = 12/23 (52%), Positives = 19/23 (82%)
Frame = -1
Query: 214 LQCLSLTYTVTWPLNIILSQEAL 146
L+ SL+Y V WPL+I++S++AL
Sbjct: 460 LETFSLSYKVQWPLSIVISKKAL 482
>At5g17410.1 68418.m02042 tubulin family protein similar to spindle
pole body protein [Homo
sapiens][GI:2801701][PMID:9566967], gamma-tubulin ring
protein Dgrip84 [Drosophila
melanogaster][GI:4689225][PMID: 10037793]
Length = 678
Score = 31.9 bits (69), Expect = 0.51
Identities = 12/23 (52%), Positives = 19/23 (82%)
Frame = -1
Query: 214 LQCLSLTYTVTWPLNIILSQEAL 146
L+ SL+Y V WPL+I++S++AL
Sbjct: 459 LETFSLSYKVQWPLSIVISKKAL 481
>At3g53760.1 68416.m05939 tubulin family protein similar to
SP|Q9SC88 Gamma-tubulin complex component 4 homolog
{Medicago truncatula}, SP|Q9UGJ1|GCP4_HUMAN
Gamma-tubulin complex component 4 {Homo sapiens};
contains Pfam profile PF04130: Spc97 / Spc98 family
Length = 745
Score = 31.5 bits (68), Expect = 0.67
Identities = 22/74 (29%), Positives = 35/74 (47%)
Frame = -2
Query: 486 LVNLDIYEHLRSLKDYFFLMDGEFSRSICHNLFSKLSKTLNPQELLNFATLHNILDKALG 307
+V D+ HL++LKDYF L G+F + C S+ L P++ + L A
Sbjct: 396 VVRADLNGHLKALKDYFLLEKGDFFQ--CFLEESRQLMRLPPRQSTGESDLMVPFQLAAT 453
Query: 306 SSISHIHKFSENVS 265
+I+ K+ VS
Sbjct: 454 KTIAEEDKYFSRVS 467
>At3g54460.1 68416.m06025 SNF2 domain-containing protein / helicase
domain-containing protein / F-box family protein similar
to SP|P36607 DNA repair protein rad8
{Schizosaccharomyces pombe}; contains Pfam profiles
PF00271: Helicase conserved C-terminal domain, PF00176:
SNF2 family N-terminal domain, PF00646: F-box domain
Length = 1378
Score = 31.1 bits (67), Expect = 0.89
Identities = 23/62 (37%), Positives = 33/62 (53%), Gaps = 5/62 (8%)
Frame = -2
Query: 390 FSKLSKTLNPQE---LLNFATLHNILDKA--LGSSISHIHKFSENVSFTITNLL*VSNTA 226
FS+LS NP++ L+ L +LD+ LGSS+S +KF VS T N ++ T
Sbjct: 808 FSRLSAEWNPRKKSPLIQVHWLRVMLDEGHTLGSSVSLTNKFQMAVSLTACNRWLLTGTP 867
Query: 225 PP 220
P
Sbjct: 868 TP 869
>At1g80260.1 68414.m09396 tubulin family protein
Length = 951
Score = 31.1 bits (67), Expect = 0.89
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = -1
Query: 220 DVLQCLSLTYTVTWPLNIILSQEAL 146
D L+ L TY V WPL +I + EA+
Sbjct: 716 DCLESLKFTYKVPWPLELIANSEAI 740
>At1g20570.1 68414.m02565 tubulin family protein
Length = 976
Score = 31.1 bits (67), Expect = 0.89
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = -1
Query: 220 DVLQCLSLTYTVTWPLNIILSQEAL 146
D L+ L TY V WPL +I + EA+
Sbjct: 755 DCLESLKFTYKVPWPLELIANSEAI 779
>At5g49110.1 68418.m06079 expressed protein ; expression supported
by MPSS
Length = 1487
Score = 30.3 bits (65), Expect = 1.5
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -2
Query: 492 YFLVNLDIYEHLRSLKDYFFLMDGEFS 412
Y L+ L+ HL+ L DYF M+G S
Sbjct: 467 YSLIMLEFVHHLKELLDYFTFMEGHIS 493
>At5g15540.1 68418.m01819 expressed protein low similarity to DNA
repair and meiosis protein Rad9 [Coprinus cinereus]
GI:1353390, SP|Q09725 Sister chromatid cohesion protein
mis4 {Schizosaccharomyces pombe}
Length = 1755
Score = 29.1 bits (62), Expect = 3.6
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +2
Query: 374 FDNLLNRL*HMLRENSPSIRKK 439
FD +LN L LREN+P+IR K
Sbjct: 774 FDKILNMLLASLRENAPNIRAK 795
>At3g47770.1 68416.m05204 ABC transporter family protein AbcA,
Dictyostelium discoideum, U66526
Length = 900
Score = 28.7 bits (61), Expect = 4.7
Identities = 14/44 (31%), Positives = 25/44 (56%), Gaps = 5/44 (11%)
Frame = +1
Query: 580 VIT*YVTGVIMFGIGAGQKYVR-----IWFLFYFLHYKSRVGVA 696
+ T Y+ +++FG G K+ R I F+FYFL+ ++ +A
Sbjct: 366 ISTLYIVCLMIFGSAIGLKFFRFNDYTIQFMFYFLYINLQISIA 409
>At5g15070.1 68418.m01766 expressed protein
Length = 1049
Score = 28.3 bits (60), Expect = 6.2
Identities = 17/53 (32%), Positives = 26/53 (49%)
Frame = +2
Query: 512 RLLAGTLVATLRNVVKHAVSVAMLSHDMLLASLCLASERVKNMFEYGFFFTSS 670
R L G ++ LRN + A+SVA L + S+ L S R ++ + F S
Sbjct: 783 RRLLGKILIDLRNTREEAMSVAELKNSQDQVSVSLYSSRKEDRYSQPKLFVKS 835
>At5g48060.1 68418.m05938 C2 domain-containing protein contains
INTERPRO:IPR000008 C2 domain
Length = 1036
Score = 27.9 bits (59), Expect = 8.3
Identities = 15/36 (41%), Positives = 18/36 (50%), Gaps = 1/36 (2%)
Frame = +3
Query: 27 PPGVLPRKFHGQFQI-LKIFSEHPGYPPHFDQKLEY 131
P +LP F F I L F P +PPH D KL +
Sbjct: 877 PELILPTMFLYMFFIGLWNFRSRPRHPPHMDMKLSW 912
>At3g43610.1 68416.m04640 tubulin family protein
Length = 1120
Score = 27.9 bits (59), Expect = 8.3
Identities = 17/52 (32%), Positives = 25/52 (48%), Gaps = 2/52 (3%)
Frame = -1
Query: 268 FVYNNESPLSFQHSSPDV--LQCLSLTYTVTWPLNIILSQEALLRTPKYSSF 119
F+Y + + S+ V L L Y V WP++IIL+ +AL SF
Sbjct: 955 FLYKRQGTMHIPPSTIGVRSFDFLRLGYRVDWPISIILTCDALTAYADVFSF 1006
>At2g13680.1 68415.m01508 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1923
Score = 27.9 bits (59), Expect = 8.3
Identities = 18/60 (30%), Positives = 30/60 (50%)
Frame = -2
Query: 747 YSKDPFTSKMDLEMKSSSYTHPALIMEEVKKKPYSNIFLTRSDAKHNDASNISCDNIATL 568
YS++ SK DLEM++ ++++ ++N FL R D K + S +NI L
Sbjct: 1080 YSEETVYSKNDLEMENEDGVSVVYYLQKIFPDEWTN-FLERLDCKDETSVLESEENILQL 1138
>At1g71020.1 68414.m08197 armadillo/beta-catenin repeat family
protein / U-box domain-containing protein contains Pfam
domain, PF00514: Armadillo/beta-catenin-like repeats and
Pfam, PF04564: U-box domain
Length = 628
Score = 27.9 bits (59), Expect = 8.3
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +3
Query: 309 RVPYPGYCVTWQNSKAPGDLT 371
R+ + CVTW+ KA GDLT
Sbjct: 104 RISFQFQCVTWKLEKALGDLT 124
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,402,487
Number of Sequences: 28952
Number of extensions: 409004
Number of successful extensions: 1151
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1151
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1804564000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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