Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NRPG0047
(698 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD... 192 3e-49
At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehy... 90 1e-18
At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate dehy... 37 0.015
At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate dehy... 37 0.015
At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate dehy... 37 0.015
At1g52190.1 68414.m05889 proton-dependent oligopeptide transport... 31 0.73
At5g51490.1 68418.m06386 pectinesterase family protein contains ... 29 3.0
At2g29770.1 68415.m03617 kelch repeat-containing F-box family pr... 29 3.0
At2g15180.1 68415.m01731 zinc knuckle (CCHC-type) family protein... 29 3.0
At4g23460.1 68417.m03381 beta-adaptin, putative strong similarit... 28 6.8
At4g39290.1 68417.m05564 kelch repeat-containing F-box family pr... 27 9.0
At2g26890.1 68415.m03226 DNAJ heat shock N-terminal domain-conta... 27 9.0
>At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD+]
/ GPDH strong similarity to SP|P52425
Glycerol-3-phosphate dehydrogenase [NAD+] (EC 1.1.1.8)
{Cuphea lanceolata}; contains Pfam profile PF01210:
NAD-dependent glycerol-3-phosphate dehydrogenase
Length = 400
Score = 192 bits (467), Expect = 3e-49
Identities = 98/203 (48%), Positives = 131/203 (64%), Gaps = 2/203 (0%)
Frame = +1
Query: 94 GSAIAKIVGRNAASLSNFEDRVTMWVYEEII-EGKKLTEIINETHENVKYLPGHKLPSNV 270
GS AK++ NA L +F D V MWV+EE++ G+KL ++IN+T+ENVKYLPG KL NV
Sbjct: 66 GSVAAKLIASNALKLPSFHDEVRMWVFEEVLPNGEKLNDVINKTNENVKYLPGIKLGRNV 125
Query: 271 VAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKGFDIAEGGGIDLI 450
VA PD+ A KDA++L+FV PHQF+ IC L GKI A+SL+KG ++ + G +I
Sbjct: 126 VADPDLENAVKDANMLVFVTPHQFMDGICKKLDGKITGDVEAISLVKGMEVKKEGPC-MI 184
Query: 451 SHIITRCLKIPCAVLMGANIASEVAEEKFCETTIGCRDVM-LAPLMRDIIQTDYFRXXXX 627
S +I++ L I C VLMGANIA+E+A EKF E T+G R +A + T YF
Sbjct: 185 SSLISKQLGINCCVLMGANIANEIAVEKFSEATVGYRGSREIADTWVQLFSTPYFMVTPV 244
Query: 628 XXXXXXXICGALKNIVAVGAGFV 696
+CG LKN+VA+ AGFV
Sbjct: 245 HDVEGVELCGTLKNVVAIAAGFV 267
>At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein low similarity to SP|Q26756
Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
(EC 1.1.1.8) {Trypanosoma brucei rhodesiense}; contains
Pfam profile PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 420
Score = 89.8 bits (213), Expect = 1e-18
Identities = 54/175 (30%), Positives = 81/175 (46%), Gaps = 3/175 (1%)
Frame = +1
Query: 181 IIEGKKLTEIINETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICS 360
++ + + INE H N KY P HKLP NV+A D A DAD + VP QF +
Sbjct: 118 LVRDSFVCQSINENHHNCKYFPEHKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLE 177
Query: 361 TLLGKIKPTAAALSLIKGFDIAEGGGIDLISHIITRCLK---IPCAVLMGANIASEVAEE 531
+ + P +SL KG ++ + ++S II LK P L G + A E+
Sbjct: 178 GIADYVDPGLPFISLSKGLEL---NTLRMMSQIIPIALKNPRQPFVALSGPSFALELMNN 234
Query: 532 KFCETTIGCRDVMLAPLMRDIIQTDYFRXXXXXXXXXXXICGALKNIVAVGAGFV 696
+ +D LA ++ ++ + Y R I GALKN++A+ AG V
Sbjct: 235 LPTAMVVASKDKKLANAVQQLLASSYLRINTSSDVTGVEIAGALKNVLAIAAGIV 289
>At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein weak similarity to
SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
(EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 455
Score = 36.7 bits (81), Expect = 0.015
Identities = 39/148 (26%), Positives = 66/148 (44%), Gaps = 8/148 (5%)
Frame = +1
Query: 277 VPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAA---LSLIKGFDIAEGGGIDL 447
V ++ EA DAD++I +P + + + K A +SL KG + +
Sbjct: 141 VTNLQEAVWDADIVINGLPSTETFQVFNEISKYWKERVNAPVIISLAKGVEAEFEPHPRI 200
Query: 448 IS--HIITRCLKIPCAVLM---GANIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDYF 612
++ +I R IP ++ G NIASEV +++ I + PL + + Q+ +F
Sbjct: 201 VTPTQMIHRATGIPLENILYLGGPNIASEVYNKEYANARICGSEKWRKPLGKFLRQS-HF 259
Query: 613 RXXXXXXXXXXXICGALKNIVAVGAGFV 696
+ G LKN+ A+GAG V
Sbjct: 260 IVWDNSDLITHEVMGGLKNVYAIGAGMV 287
>At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein weak similarity to
SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
(EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 462
Score = 36.7 bits (81), Expect = 0.015
Identities = 39/152 (25%), Positives = 64/152 (42%), Gaps = 12/152 (7%)
Frame = +1
Query: 277 VPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAA---LSLIKGFDIAEGGGIDL 447
V ++ EA DAD+++ +P R + + K +SL KG + A ++
Sbjct: 149 VTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLSKGIETA----LEP 204
Query: 448 ISHIITRCLKIPCAV---------LMGANIASEVAEEKFCETTIGCRDVMLAPLMRDIIQ 600
+ HIIT I A L G NIA+E+ +++ I PL + ++
Sbjct: 205 VPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGAAKWRKPLAK-FLR 263
Query: 601 TDYFRXXXXXXXXXXXICGALKNIVAVGAGFV 696
+F + G LKN+ A+GAG V
Sbjct: 264 QPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMV 295
>At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein weak similarity to
SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
(EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 462
Score = 36.7 bits (81), Expect = 0.015
Identities = 39/152 (25%), Positives = 64/152 (42%), Gaps = 12/152 (7%)
Frame = +1
Query: 277 VPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAA---LSLIKGFDIAEGGGIDL 447
V ++ EA DAD+++ +P R + + K +SL KG + A ++
Sbjct: 149 VTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLSKGIETA----LEP 204
Query: 448 ISHIITRCLKIPCAV---------LMGANIASEVAEEKFCETTIGCRDVMLAPLMRDIIQ 600
+ HIIT I A L G NIA+E+ +++ I PL + ++
Sbjct: 205 VPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGAAKWRKPLAK-FLR 263
Query: 601 TDYFRXXXXXXXXXXXICGALKNIVAVGAGFV 696
+F + G LKN+ A+GAG V
Sbjct: 264 QPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMV 295
>At1g52190.1 68414.m05889 proton-dependent oligopeptide transport
(POT) family protein contains Pfam profile: PF00854 POT
family
Length = 607
Score = 31.1 bits (67), Expect = 0.73
Identities = 15/51 (29%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +1
Query: 22 ARGKNFP-LWSLLGHYIPIYNIFYRGSAIAKIVGRNAASLSNFEDRVTMWV 171
A+G N +WS ++ P+ F S + + + + ASLS+F V +W+
Sbjct: 62 AKGTNVLFMWSAASNFTPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWL 112
>At5g51490.1 68418.m06386 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 536
Score = 29.1 bits (62), Expect = 3.0
Identities = 15/47 (31%), Positives = 24/47 (51%)
Frame = +1
Query: 448 ISHIITRCLKIPCAVLMGANIASEVAEEKFCETTIGCRDVMLAPLMR 588
ISH+I+ CL + A+L N + A +K T + +D L +R
Sbjct: 173 ISHLISNCLAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRAVR 219
>At2g29770.1 68415.m03617 kelch repeat-containing F-box family
protein contains Pfam PF00646: F-box domain; contains
Pfam PF01344 : Kelch motif; similar to SKP1 interacting
partner 6 (GI:10716957) [Arabidopsis thaliana]
Length = 387
Score = 29.1 bits (62), Expect = 3.0
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +3
Query: 492 INGSQYCIGGC*GKILRNDHWL 557
I+G Y IGGC + RNDHW+
Sbjct: 181 IDGRIYVIGGC---VKRNDHWI 199
>At2g15180.1 68415.m01731 zinc knuckle (CCHC-type) family protein
contains Pfam domain PF00098: Zinc knuckle
Length = 474
Score = 29.1 bits (62), Expect = 3.0
Identities = 9/29 (31%), Positives = 14/29 (48%)
Frame = +2
Query: 611 SGSWWWTMRTQSKYVER*RTLWQWVRVSW 697
S WWW +++ ER + W R+ W
Sbjct: 151 SALWWWDQDEYNRWYERRAPIRTWERLKW 179
>At4g23460.1 68417.m03381 beta-adaptin, putative strong similarity
to SP|Q10567 Adapter-related protein complex 1 beta 1
subunit (Beta-adaptin 1) [Homo sapiens], beta-adaptin
[Drosophila melanogaster] GI:434902; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 893
Score = 27.9 bits (59), Expect = 6.8
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -1
Query: 578 GASITSRQPMVVSQNFSSATSDAIL 504
GAS + PMV+SQN S+ ++ ++L
Sbjct: 733 GASARTMMPMVLSQNMSTGSTSSVL 757
>At4g39290.1 68417.m05564 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 365
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +3
Query: 480 SLCCINGSQYCIGGC*GKILRNDHWL 557
S C +NG Y IGGC K L + +W+
Sbjct: 160 STCVLNGKIYVIGGC--KNLDSTNWI 183
>At2g26890.1 68415.m03226 DNAJ heat shock N-terminal domain-containing
protein contains Pfam profile PF00226: DnaJ domain
Length = 2554
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +1
Query: 385 TAAALSLIKGFDIAEGGGIDLISHIITRCL 474
T AA SL G ++ GG+ L+S +++RC+
Sbjct: 1652 TCAASSL-NGEELVRDGGVQLLSTLLSRCM 1680
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,326,136
Number of Sequences: 28952
Number of extensions: 275356
Number of successful extensions: 817
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 794
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 812
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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