Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_B01
(909 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g28290.1 68414.m03472 pollen Ole e 1 allergen and extensin fa... 33 0.26
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 31 1.1
At1g62250.2 68414.m07023 expressed protein 30 1.8
At1g62250.1 68414.m07022 expressed protein 30 1.8
At5g09400.1 68418.m01089 potassium transporter family protein si... 29 4.3
At1g02405.1 68414.m00187 proline-rich family protein contains pr... 29 4.3
At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (AT... 29 5.6
At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A... 29 5.6
At3g62000.1 68416.m06963 O-methyltransferase family 3 protein se... 28 9.8
At3g04160.1 68416.m00440 expressed protein ; expression supporte... 28 9.8
At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1) domain... 28 9.8
>At1g28290.1 68414.m03472 pollen Ole e 1 allergen and extensin
family protein similar to arabinogalactan protein
[Daucus carota] GI:11322245; contains Pfam profile
PF01190: Pollen proteins Ole e I family
Length = 359
Score = 33.1 bits (72), Expect = 0.26
Identities = 32/117 (27%), Positives = 39/117 (33%)
Frame = +3
Query: 495 VTPKTKPARKSPGSLPPCWKTXXFTSRSCXPRTNST*SSITRKVLVMTVSSIVIAPLTXX 674
V P TKP K P S PP P T + T+ + V AP+
Sbjct: 100 VKPPTKPPVKPPVS-PPAKPP---VKPPVYPPTKAPVKPPTKPPVKPPVYPPTKAPVKPP 155
Query: 675 PHSYLEPSXYXATSCFXSPPTVPXCXXPXXKIGRPPNXXPXGPXETSXPXLXXXXPP 845
++P Y T PPT P P +PP P P T P PP
Sbjct: 156 TKPPVKPPVYPPTKAPVKPPTKPPVKPPVSPPAKPPVKPPVYP-PTKAPVKPPVSPP 211
Score = 30.7 bits (66), Expect = 1.4
Identities = 19/65 (29%), Positives = 24/65 (36%)
Frame = +3
Query: 651 VIAPLTXXPHSYLEPSXYXATSCFXSPPTVPXCXXPXXKIGRPPNXXPXGPXETSXPXLX 830
V AP++ ++P Y T PPT P P +PP P P T P
Sbjct: 76 VKAPVSPPAKPPVKPPVYPPTKAPVKPPTKPPVKPPVSPPAKPPVKPPVYP-PTKAPVKP 134
Query: 831 XXXPP 845
PP
Sbjct: 135 PTKPP 139
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 31.1 bits (67), Expect = 1.1
Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 4/85 (4%)
Frame = +2
Query: 209 IGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDG--KEIVKSYF 382
+G Y+ A+ S + G I++ K LIE+ + T+D +L T+ G + S
Sbjct: 498 LGNYDEALQYVSSLEPSQAGVTIEQYGKILIEHKPKETIDILMRLCTEQGIPNGVFLSML 557
Query: 383 --PIQFRVIFTEQTVKLINKRDHHA 451
P+ F +F + L++ + +A
Sbjct: 558 PSPVDFITVFVQHPHSLMHFLERYA 582
>At1g62250.2 68414.m07023 expressed protein
Length = 223
Score = 30.3 bits (65), Expect = 1.8
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +3
Query: 105 LCLPSSSPCVRWLLTPHLHQELMTYWRSSCI*VSSLVNTRPLS 233
LC P + +RW TP + E+++ WR C +++ R ++
Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222
>At1g62250.1 68414.m07022 expressed protein
Length = 267
Score = 30.3 bits (65), Expect = 1.8
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +3
Query: 105 LCLPSSSPCVRWLLTPHLHQELMTYWRSSCI*VSSLVNTRPLS 233
LC P + +RW TP + E+++ WR C +++ R ++
Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222
>At5g09400.1 68418.m01089 potassium transporter family protein
similar to K+ transporter HAK5 [Arabidopsis thaliana]
GI:7108597; contains Pfam profile PF02705: K+ potassium
transporter; KUP/HAK/KT Transporter family member,
PMID:11500563; Note: possible sequencing error causes a
frameshift in the 4th exon|15810448|gb|AY056263
Length = 858
Score = 29.1 bits (62), Expect = 4.3
Identities = 19/73 (26%), Positives = 35/73 (47%)
Frame = -1
Query: 291 FTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVALEASAHTARTKA 112
F +S+ S F+ + ++A ++ MTT +SC S+ LG L+ HT+R
Sbjct: 411 FFSSVPGSAFWPVLFIANIAALIASRTMTTATFSC-IKQSTALGCFPRLKI-IHTSRKFM 468
Query: 111 NKVSLILAQWLSL 73
++ + + W L
Sbjct: 469 GQIYIPVLNWFLL 481
>At1g02405.1 68414.m00187 proline-rich family protein contains
proline-rich region, INTERPRO:IPR000694
Length = 134
Score = 29.1 bits (62), Expect = 4.3
Identities = 14/47 (29%), Positives = 14/47 (29%)
Frame = +2
Query: 755 PLXXNWPPPXPXTXGPXXNLXSPXXXXXPPXSXXXXPXXXXXXPXPP 895
P N PPP P P P PP P P PP
Sbjct: 43 PCLQNQPPPPPSPPPPSCTPSPPPPSPPPPKKSSCPPSPLPPPPPPP 89
>At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase
(ATHIM) identical to SP|P34881 DNA
(cytosine-5)-methyltransferase AthI (EC 2.1.1.37)
{Arabidopsis thaliana}
Length = 1534
Score = 28.7 bits (61), Expect = 5.6
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -2
Query: 353 PLSITGRRSPWCSSCRFRSDASR 285
PLS GR S +C+SC+ R D +
Sbjct: 869 PLSDIGRSSGFCTSCKIREDEEK 891
>At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A19)
Identical to Cytochrome P450 (SP:Q9T0K0) [Arabidopsis
thaliana]; similar to cytochrome P450LXXIA1, Persea
americana, M32885
Length = 490
Score = 28.7 bits (61), Expect = 5.6
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -3
Query: 451 GVMVPFVYELDSLLGEDHSKLDGEVRFDDFL 359
G +P + +D + G+DH + + RFD+FL
Sbjct: 221 GEYIPSLSWIDKIRGQDHKMEEVDKRFDEFL 251
>At3g62000.1 68416.m06963 O-methyltransferase family 3 protein
several O-methyltransferases - different species;
contains Pfam 01596 O-methyltransferase domain
Length = 278
Score = 27.9 bits (59), Expect = 9.8
Identities = 26/126 (20%), Positives = 56/126 (44%), Gaps = 2/126 (1%)
Frame = +2
Query: 176 VLAEQLYMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKD 355
V E+ S+ + + +A S + K+G + E++K +I+NG+ + DFA+
Sbjct: 147 VACERDSNSLEVAKRYYELAGVSHKVNVKQG-LAAESLKSMIQNGEGASYDFAF---VDA 202
Query: 356 GKEIVKSYFPIQFRVIFTEQTVKLINKRDHHALK--LIDQQNHNKIAFGDSKDKTSKKVS 529
K + + YF + +++ + + N H + +++ I + K K+VS
Sbjct: 203 DKRMYQDYFELLLQLVRVGGVIVMDNVLWHGRVSDPMVNDAKTISIRNFNKKLMDDKRVS 262
Query: 530 WKFTPV 547
P+
Sbjct: 263 ISMVPI 268
>At3g04160.1 68416.m00440 expressed protein ; expression supported
by MPSS
Length = 712
Score = 27.9 bits (59), Expect = 9.8
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = +1
Query: 283 SREASDRKRQEEHHGLRLPVMDKGWKGNRQILLPHPV 393
SR ++E G +P DK WKG + + +P+
Sbjct: 563 SRSPIGNDQKESDFGYSIPSTDKQWKGENRADIEYPI 599
>At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein / tetratricopeptide repeat
(TPR)-containing protein low similarity to SP|Q99614
Tetratricopeptide repeat protein 1 {Homo sapiens};
contains Pfam profiles PF00564: PB1 domain, PF00515: TPR
Domain
Length = 697
Score = 27.9 bits (59), Expect = 9.8
Identities = 17/49 (34%), Positives = 25/49 (51%)
Frame = +2
Query: 194 YMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQ 340
YM + +GEY AI +C+ L E K +KR N +DFA++
Sbjct: 100 YMQMGLGEYPNAINECNLAL-EASPRFSKALLKRARCYEALNKLDFAFR 147
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,736,661
Number of Sequences: 28952
Number of extensions: 352959
Number of successful extensions: 1401
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1040
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1289
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2149324008
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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