Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0001_E01
         (436 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str...   143   4e-35
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S...   142   1e-34
At2g18470.1 68415.m02151 protein kinase family protein contains ...    29   1.0  
At1g41900.1 68414.m04840 myosin heavy chain-related similar to M...    27   7.2  
At5g64590.1 68418.m08117 hypothetical protein contains Pfam prof...    26   9.5  

>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
           similarity to 60S ribosomal protein L1 GB:P49691
          Length = 406

 Score =  143 bits (347), Expect = 4e-35
 Identities = 67/146 (45%), Positives = 88/146 (60%), Gaps = 1/146 (0%)
 Frame = +2

Query: 2   FAPTKPWRRWHXXXXXXXXXXXXXXXXXXXXXXXXXQARGHIVEKIPELPLVVSDKVQEI 181
           FAPTK WRRWH                          ARGH +E +PE+PLVVSD  + +
Sbjct: 107 FAPTKIWRRWHRRVNVNMKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAV 166

Query: 182 NKTKQAVIFLRRIKAWSDVLKVYKSQRLRAGKGKMRNRRRVQRKGPLIIYNKD-KGLTRA 358
            KT  A+  L++I A+ D  K   S  +R GKGKMRNRR + RKGPL++Y  +   + +A
Sbjct: 167 EKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKGPLVVYGTEGSKIVKA 226

Query: 359 FRNIPCIEMLNVNQLNLLKLAPGGHL 436
           FRN+P +E+ +V +LNLLKLAPGGHL
Sbjct: 227 FRNLPGVELCHVERLNLLKLAPGGHL 252


>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
           roibosomal protein L4, Arabidopsis thaliana,
           EMBL:CAA79104
          Length = 407

 Score =  142 bits (343), Expect = 1e-34
 Identities = 66/146 (45%), Positives = 88/146 (60%), Gaps = 1/146 (0%)
 Frame = +2

Query: 2   FAPTKPWRRWHXXXXXXXXXXXXXXXXXXXXXXXXXQARGHIVEKIPELPLVVSDKVQEI 181
           FAPTK WRRWH                          ARGH +E +PE+PLVVSD  + +
Sbjct: 108 FAPTKIWRRWHRRVNVNMKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAV 167

Query: 182 NKTKQAVIFLRRIKAWSDVLKVYKSQRLRAGKGKMRNRRRVQRKGPLIIYNKDKG-LTRA 358
            KT  A+  L++I A+ D  K   S  +R GKGKMRNRR + RKGPL+++  +   + +A
Sbjct: 168 EKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKGPLVVFGTEGAKIVKA 227

Query: 359 FRNIPCIEMLNVNQLNLLKLAPGGHL 436
           FRN+P +E+ +V +LNLLKLAPGGHL
Sbjct: 228 FRNLPGVELCHVERLNLLKLAPGGHL 253


>At2g18470.1 68415.m02151 protein kinase family protein contains
           Pfam PF00069: Protein kinase domain
          Length = 633

 Score = 29.5 bits (63), Expect = 1.0
 Identities = 14/40 (35%), Positives = 20/40 (50%)
 Frame = -1

Query: 139 NLLNNMPSSLNEGRYPGRCHGGGHGSTSLP*VHATVPAPP 20
           N  NN PS  ++G    R +GG +G    P    + P+PP
Sbjct: 66  NSSNNSPSPPSQGGGGERGNGGNNGGNDTPPSRGSPPSPP 105


>At1g41900.1 68414.m04840 myosin heavy chain-related similar to
           Myosin heavy chain, skeletal muscle, extraocular
           (MyHC-eo) (SP:Q9UKX3)  {Homo sapiens}
          Length = 442

 Score = 26.6 bits (56), Expect = 7.2
 Identities = 13/35 (37%), Positives = 20/35 (57%)
 Frame = +2

Query: 263 LRAGKGKMRNRRRVQRKGPLIIYNKDKGLTRAFRN 367
           L+ G  K R+R  VQ +G + +Y+K  G    FR+
Sbjct: 364 LQTGLRKRRSRGAVQVEGGIRLYSKQVGSPAGFRD 398


>At5g64590.1 68418.m08117 hypothetical protein contains Pfam profile
           PF04396: Protein of unknown function, DUF537
          Length = 206

 Score = 26.2 bits (55), Expect = 9.5
 Identities = 15/33 (45%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
 Frame = -1

Query: 127 NMPSSL-NEGRYPGRCHGGGHGSTSLP*VHATV 32
           NM  +L N G Y GRC   G G  SL   HA +
Sbjct: 34  NMRMALANNGEYLGRCEITGFGDYSLATKHAGI 66


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,236,324
Number of Sequences: 28952
Number of extensions: 141713
Number of successful extensions: 380
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 371
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 376
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 683042040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)