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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0001_C21
         (455 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl...    28   3.4  
At5g15540.1 68418.m01819 expressed protein low similarity to DNA...    27   4.5  
At2g43270.1 68415.m05378 F-box family protein contains Pfam PF00...    27   4.5  
At5g40480.1 68418.m04909 expressed protein ; expression supporte...    27   6.0  
At4g17830.1 68417.m02659 peptidase M20/M25/M40 family protein si...    27   6.0  
At1g56590.1 68414.m06508 clathrin adaptor complexes medium subun...    27   6.0  
At3g21550.1 68416.m02718 expressed protein contains Pfam profile...    27   7.9  

>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
           identical to ubiquitin-protein ligase 2 [Arabidopsis
           thaliana] GI:7108523; E3, HECT-domain protein family;
           similar to ubiquitin-protein ligase 2 GI:7108523 from
           [Arabidopsis thaliana]
          Length = 3658

 Score = 27.9 bits (59), Expect = 3.4
 Identities = 14/35 (40%), Positives = 23/35 (65%)
 Frame = +1

Query: 70  LNETIAQYPTNKDVARTFDIMQTDLQCCGINGPED 174
           L++TI  +   ++V+RT D ++  + C G NGPED
Sbjct: 442 LDDTI--FRLKQEVSRTEDDVKEIVCCSGSNGPED 474


>At5g15540.1 68418.m01819 expressed protein low similarity to DNA
            repair and meiosis protein Rad9 [Coprinus cinereus]
            GI:1353390, SP|Q09725 Sister chromatid cohesion protein
            mis4 {Schizosaccharomyces pombe}
          Length = 1755

 Score = 27.5 bits (58), Expect = 4.5
 Identities = 25/94 (26%), Positives = 37/94 (39%), Gaps = 2/94 (2%)
 Frame = +1

Query: 40   KDLETSIVKHLNETIAQYPTNKDVARTFDIMQTDLQCCGINGPEDWAAHNLTIPNTC--C 213
            +DL+  IV+H   T+     +K   +   I++  LQ       E   + N  I      C
Sbjct: 1096 QDLKHMIVRHSFLTVVHACVSKLAGKGVSIVEHLLQFF-FKRLEAQGSDNTQIAGRSLFC 1154

Query: 214  TGQEINDGVLVACTKDTPGFHTKGCLNELVAHLK 315
             G  I  G  +  T     F+  GCLN    HL+
Sbjct: 1155 LGLLIRHGNSLISTSGGKNFNLSGCLNLFKRHLR 1188


>At2g43270.1 68415.m05378 F-box family protein contains Pfam
           PF00646: F-box domain; contains TIGRFAM TIGR01640 :
           F-box protein interaction domain
          Length = 387

 Score = 27.5 bits (58), Expect = 4.5
 Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 2/27 (7%)
 Frame = +1

Query: 184 HNLTIPNTCCT--GQEINDGVLVACTK 258
           HN+++P+TCCT   Q +N    +A TK
Sbjct: 210 HNVSVPSTCCTMSAQMVNLDDRLAITK 236


>At5g40480.1 68418.m04909 expressed protein ; expression supported
           by MPSS
          Length = 1919

 Score = 27.1 bits (57), Expect = 6.0
 Identities = 16/57 (28%), Positives = 29/57 (50%)
 Frame = -3

Query: 339 SSEHYSDILEVSDQFVQAAFSMETGCVLSASDQNTIVDLLSGAASVRDGQVVRCPVF 169
           S +H+ DIL V+ +F  ++    +  + S S  +   +    A  ++ G V+RC VF
Sbjct: 59  SWDHH-DILSVTPEFNSSSHCSTSARLRSISPYSGRKETAVYATDIQTGMVIRCKVF 114


>At4g17830.1 68417.m02659 peptidase M20/M25/M40 family protein
           similar to acetylornithine deacetylase
           (Acetylornithinase, AO; N-acetylornithinase, NAO)
           [Dictyostelium discoideum] SWISS-PROT:P54638
          Length = 440

 Score = 27.1 bits (57), Expect = 6.0
 Identities = 12/25 (48%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
 Frame = +1

Query: 244 VACTKDTPGFHTK-GCLNELVAHLK 315
           VAC  D+PGFH       E+V H+K
Sbjct: 355 VACNLDSPGFHVLCKATEEVVGHVK 379


>At1g56590.1 68414.m06508 clathrin adaptor complexes medium subunit
           family protein contains Pfam profile: PF00928 adaptor
           complexes medium subunit family
          Length = 415

 Score = 27.1 bits (57), Expect = 6.0
 Identities = 14/47 (29%), Positives = 24/47 (51%), Gaps = 2/47 (4%)
 Frame = -3

Query: 249 SDQNTIVDLLSGAASV--RDGQVVRCPVFGAVDTATL*VGLHDVERS 115
           S     VDL+    ++  RDG++V+C ++G V   +   G  D+  S
Sbjct: 177 SSNEVYVDLVEEMDAIVNRDGELVKCEIYGEVQMNSQLTGFPDLTLS 223


>At3g21550.1 68416.m02718 expressed protein contains Pfam profile
           PF05078: Protein of unknown function (DUF679)
          Length = 184

 Score = 26.6 bits (56), Expect = 7.9
 Identities = 25/68 (36%), Positives = 32/68 (47%), Gaps = 3/68 (4%)
 Frame = -3

Query: 345 PDSS--EHYSDILEVSDQFVQAAFSMETGCVLSASDQNTIVDLLSGAASV-RDGQVVRCP 175
           PDSS  +  S  L V D FV A FS+    V+S  D NT+     G  S  +   +V  P
Sbjct: 91  PDSSSVDLSSKRLRVGD-FVHAFFSLIVFSVISLLDANTVNCFYPGFGSAGKIFLMVLPP 149

Query: 174 VFGAVDTA 151
           V G +  A
Sbjct: 150 VIGVISGA 157


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,515,607
Number of Sequences: 28952
Number of extensions: 182461
Number of successful extensions: 540
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 529
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 540
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 752336160
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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