Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV40992
         (664 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    77   3e-15
SPAC4A8.10 |||lipase |Schizosaccharomyces pombe|chr 1|||Manual         28   1.0  
SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc...    27   2.4  
SPBC1685.13 |||non classical export pathway protein |Schizosacch...    26   4.2  
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    26   5.6  
SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1||...    26   5.6  
SPAC3F10.09 |||1-|Schizosaccharomyces pombe|chr 1|||Manual             25   7.4  
SPBC646.14c |orc5||origin recognition complex subunit Orc5|Schiz...    25   9.7  
SPBC887.02 |||ClC chloride channel|Schizosaccharomyces pombe|chr...    25   9.7  
SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po...    25   9.7  
SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha...    25   9.7  
SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr...    25   9.7  

>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 76.6 bits (180), Expect = 3e-15
 Identities = 33/50 (66%), Positives = 42/50 (84%)
 Frame = +3

Query: 510 WGASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEKIQEFEDFVQSVDIAA 659
           WG SKLVPVG+G+NK QI  V+EDDKVS++ L E+++ FED+VQS DIAA
Sbjct: 161 WGLSKLVPVGFGVNKFQINLVVEDDKVSLEALQEELEGFEDYVQSTDIAA 210



 Score = 67.3 bits (157), Expect = 2e-12
 Identities = 32/66 (48%), Positives = 41/66 (62%), Gaps = 1/66 (1%)
 Frame = +2

Query: 8   MAVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAP-AANLPHVLRWYNQI 184
           M   D+ +  GL  LN +L +KS++ GY PSQAD  VF+ VG AP  A  P+  RWY QI
Sbjct: 1   MGFSDLTSDAGLKQLNDFLLDKSFIEGYEPSQADAVVFKAVGVAPDTAKYPNGARWYKQI 60

Query: 185 ASYTSA 202
           A+Y  A
Sbjct: 61  ATYDLA 66



 Score = 60.5 bits (140), Expect = 2e-10
 Identities = 26/45 (57%), Positives = 33/45 (73%)
 Frame = +1

Query: 373 YADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGL 507
           Y  KK+ KP  + KS + LDVKPWDDET M E+E  VR+I+M+GL
Sbjct: 115 YNKKKAAKPKAVHKSLVTLDVKPWDDETPMDELEKAVRSIQMDGL 159


>SPAC4A8.10 |||lipase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 723

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 2/35 (5%)
 Frame = -2

Query: 558 AVYLCHTQQAQVWRLPRKAFHFNSAYLVF--HFLH 460
           A+++ H     +WRLP  +FH  +++LV   H +H
Sbjct: 219 ALHVEHQTPENLWRLPYSSFHSKNSHLVVLTHGMH 253


>SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1063

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 13/40 (32%), Positives = 21/40 (52%)
 Frame = +2

Query: 236 LTAGAKPRLPPQQRKTTMTTTLIYLVLVTRKRMQKLNEFE 355
           L  G   RLPP+Q K        +L ++ + R Q+LN+ +
Sbjct: 377 LELGQSQRLPPEQLKLIGMLYRKHLQIILQHRQQQLNKIQ 416


>SPBC1685.13 |||non classical export pathway protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 183

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 13/35 (37%), Positives = 19/35 (54%)
 Frame = -3

Query: 593 RNFVVLDDTHNLQFIYAIPNRHKFGGSPGRPSISI 489
           R F +  DT  L    A+ N+   GGSPG+ + S+
Sbjct: 15  RTFQLAIDTIVLALASALVNQQTSGGSPGKINFSV 49


>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 513

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 12/40 (30%), Positives = 21/40 (52%)
 Frame = +2

Query: 176 NQIASYTSAERKTWSQGTSPLTAGAKPRLPPQQRKTTMTT 295
           NQ  SY ++ +  + Q TSP+ + +    PP  R +  T+
Sbjct: 213 NQPPSYAASTQPEFQQTTSPIFSSSSTPPPPPPRPSQPTS 252


>SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1101

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 22/57 (38%), Positives = 27/57 (47%), Gaps = 6/57 (10%)
 Frame = -3

Query: 650 VNRLYKIFKFLNFFCQQINRNFVVLDDTHNL-QFIYAI-----PNRHKFGGSPGRPS 498
           +N L  I + L   C   NRNF  LD   NL Q + +       +R  FGG PG PS
Sbjct: 684 LNYLINIKRLL--LCPFSNRNFSSLDLYSNLIQHLLSANSSPRKSRLSFGGRPGSPS 738


>SPAC3F10.09 |||1-|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 264

 Score = 25.4 bits (53), Expect = 7.4
 Identities = 11/27 (40%), Positives = 18/27 (66%)
 Frame = +3

Query: 546 INKLQIMCVIEDDKVSVDLLTEKIQEF 626
           INK Q+M   E ++ ++DLL++   EF
Sbjct: 151 INKWQVMTAFELNEENLDLLSQYCSEF 177


>SPBC646.14c |orc5||origin recognition complex subunit
           Orc5|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 455

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 9/27 (33%), Positives = 18/27 (66%)
 Frame = +2

Query: 41  LNDLNQYLAEKSYVSGYTPSQADVQVF 121
           L+ +++YL   ++++ Y PS+ D Q F
Sbjct: 300 LSLVSKYLLVSAFLASYNPSRLDAQFF 326


>SPBC887.02 |||ClC chloride channel|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 667

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 12/37 (32%), Positives = 19/37 (51%)
 Frame = -1

Query: 229 GALRPCFAFSRSV*SNLIIPS*YVGKVSGRRLANLLK 119
           G L     F  ++ + +I+PS  +G   GR +  LLK
Sbjct: 394 GLLLTSATFGAAIPTGIIVPSLAIGACIGRAVGTLLK 430


>SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 811

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 11/32 (34%), Positives = 19/32 (59%)
 Frame = +1

Query: 379 DKKSKKPALIAKSSILLDVKPWDDETDMKEME 474
           DK+S  P LIA++ +  ++   D+   +KE E
Sbjct: 692 DKESNSPVLIAETRLFNNLFKCDNPAALKEQE 723


>SPAC13G7.04c |mac1||membrane anchored protein Mac1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 756

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 10/17 (58%), Positives = 12/17 (70%)
 Frame = -3

Query: 530 HKFGGSPGRPSISIVRT 480
           HK G  PG PS++I RT
Sbjct: 313 HKTGNRPGFPSLNIPRT 329


>SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 475

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 11/27 (40%), Positives = 14/27 (51%)
 Frame = -3

Query: 179 DYTIVVRGEG*RPAPCQLAQILEHQLE 99
           +Y +VV G G  P  C +   L  QLE
Sbjct: 308 NYPVVVNGNGVNPYLCDVQAFLTSQLE 334


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,543,311
Number of Sequences: 5004
Number of extensions: 50190
Number of successful extensions: 154
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)