Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV40532
         (782 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ288391-1|ABC41341.1|  630|Apis mellifera vasa protein protein.       54   1e-09
L10710-1|AAA27730.1|  382|Apis mellifera hyaluronidase protein.        25   0.79 
DQ026038-1|AAY87897.1|  520|Apis mellifera nicotinic acetylcholi...    23   2.4  
AB238796-1|BAE93398.1|  128|Apis mellifera Queen brain-selective...    23   2.4  
DQ026032-1|AAY87891.1|  566|Apis mellifera nicotinic acetylcholi...    22   5.6  

>DQ288391-1|ABC41341.1|  630|Apis mellifera vasa protein protein.
          Length = 630

 Score = 54.4 bits (125), Expect = 1e-09
 Identities = 29/84 (34%), Positives = 47/84 (55%), Gaps = 5/84 (5%)
 Frame = +3

Query: 501 CSWVVGTPGRVYDMITRRALHANTIKLFVLDEADEMLSRGFKDQI-----HDVFKMLSAD 665
           C  +V TPGR+ D + +  +  ++++  VLDEAD ML  GF   I     H+    L  +
Sbjct: 324 CHILVATPGRLLDFVEKGRVKFSSVQFLVLDEADRMLDMGFLPSIEKMVDHETMVPL-GE 382

Query: 666 VQVILLSATMPDDVLEVSRCFMRD 737
            Q ++ SAT PD+V  ++R F+ +
Sbjct: 383 RQTLMFSATFPDEVQHLARRFLNN 406



 Score = 52.0 bits (119), Expect = 6e-09
 Identities = 32/93 (34%), Positives = 52/93 (55%), Gaps = 9/93 (9%)
 Frame = +1

Query: 256 GRDVIAQAQSGTGKTATFSISIL-----QQIDTSIR----ECQALILAPTRELAQQIQKV 408
           GRD++A AQ+G+GKTA F++ I+     + +D  +     E Q +I++PTREL  QI + 
Sbjct: 233 GRDLMACAQTGSGKTAAFAVPIINTLLERSVDLVVTSTYCEPQVVIVSPTRELTIQIWQQ 292

Query: 409 VIALGDHLNAKCHACIGGTNVREDIRQLESGVH 507
           ++    +   K     GGT+V     +L +G H
Sbjct: 293 IVKFSLNSILKTVVAYGGTSVMHQRGKLSAGCH 325



 Score = 27.5 bits (58), Expect = 0.15
 Identities = 11/35 (31%), Positives = 22/35 (62%)
 Frame = +2

Query: 137 QVVETFDDMNLKXELLRGIYAYGFEKPSAIQQRAI 241
           Q +E+F+   L+  +L  I   G++KP+ +Q+ A+
Sbjct: 193 QPIESFEAAGLRNIVLDNIKKSGYKKPTPVQKHAL 227


>L10710-1|AAA27730.1|  382|Apis mellifera hyaluronidase protein.
          Length = 382

 Score = 25.0 bits (52), Expect = 0.79
 Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
 Frame = -1

Query: 626 LETSGQHFISFIKNKKFDGVGMKRTTSYHIIY-TTWSAHHP*TPLS 492
           L+    H I+ I +K F GVG+    S+  I+   W++  P   LS
Sbjct: 120 LQVFRDHLINQIPDKSFPGVGVIDFESWRPIFRQNWASLQPYKKLS 165


>DQ026038-1|AAY87897.1|  520|Apis mellifera nicotinic acetylcholine
           receptor beta1subunit protein.
          Length = 520

 Score = 23.4 bits (48), Expect = 2.4
 Identities = 10/25 (40%), Positives = 17/25 (68%)
 Frame = +2

Query: 281 SQELEKLLLSLYRFYNKSIQAFVNV 355
           S++ E+L+  L+R YNK I+   N+
Sbjct: 24  SEDEERLVRDLFRGYNKLIRPVQNM 48


>AB238796-1|BAE93398.1|  128|Apis mellifera Queen brain-selective
           protein-1 protein.
          Length = 128

 Score = 23.4 bits (48), Expect = 2.4
 Identities = 9/15 (60%), Positives = 11/15 (73%)
 Frame = -2

Query: 472 GHWCHQCKHGI*HSS 428
           G  CH+CK+GI  SS
Sbjct: 40  GDSCHKCKYGIAMSS 54


>DQ026032-1|AAY87891.1|  566|Apis mellifera nicotinic acetylcholine
           receptor alpha3subunit protein.
          Length = 566

 Score = 22.2 bits (45), Expect = 5.6
 Identities = 6/8 (75%), Positives = 6/8 (75%)
 Frame = +3

Query: 450 LHWWHQCP 473
           LH WH CP
Sbjct: 470 LHHWHHCP 477


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 238,307
Number of Sequences: 438
Number of extensions: 5561
Number of successful extensions: 20
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 24639531
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)