BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40332
(741 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC23G7.05 |sui1|psu1|translation initiation factor eIF1|Schizo... 57 2e-09
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 32 0.099
SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3 |Schizos... 28 1.2
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 27 3.7
SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr 1... 26 4.9
SPCC663.14c |||TRP-like ion channel|Schizosaccharomyces pombe|ch... 25 8.6
>SPBC23G7.05 |sui1|psu1|translation initiation factor
eIF1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 109
Score = 57.2 bits (132), Expect = 2e-09
Identities = 29/55 (52%), Positives = 36/55 (65%), Gaps = 1/55 (1%)
Frame = +3
Query: 132 SIQNLNTFDPFADAIKSSEDDVQD-GLVHVRIQQRNGRKTLTRCKAFPRNMT*RR 293
+IQN NT DPFAD + +DD Q +H+RIQQRNGRKTLT + PR +R
Sbjct: 3 AIQNFNTVDPFAD---TGDDDAQPLNNIHIRIQQRNGRKTLTTVQGLPREFDQKR 54
Score = 47.2 bits (107), Expect = 2e-06
Identities = 19/43 (44%), Positives = 32/43 (74%)
Frame = +1
Query: 328 GTVVEHPEYGEVLQLQGDQRENICQWLTKSGLVKPEQLKVHGF 456
GT+V+ + GEV+QLQGDQR + ++LT+ ++ + +K+HGF
Sbjct: 67 GTIVKDDDLGEVIQLQGDQRIKVMEFLTQQLGLQKKNIKIHGF 109
Score = 36.7 bits (81), Expect = 0.003
Identities = 15/24 (62%), Positives = 20/24 (83%)
Frame = +2
Query: 254 SVQGLSSEYDLKKIVRACKKEFAC 325
+VQGL E+D K+I+RA KK+FAC
Sbjct: 42 TVQGLPREFDQKRILRALKKDFAC 65
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 31.9 bits (69), Expect = 0.099
Identities = 20/46 (43%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
Frame = -2
Query: 371 SCSTSPYSGCSTTVPCTRTPSCMPARSSSGHIPRKGLAPS-QRLTP 237
SCS PY S T RTPS + SS P+ L+PS + LTP
Sbjct: 205 SCSPEPYLPLSPTRSPARTPSPIRLYSSDALRPQSPLSPSVEYLTP 250
>SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 790
Score = 28.3 bits (60), Expect = 1.2
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = -2
Query: 191 VLRAFDSIGEWVECVEILDGHTIERRVSLFQR 96
+LR S+ W+ ++ +G T+E+RV LF+R
Sbjct: 25 LLRDPYSLKSWLRYIKTHEGSTLEKRVLLFER 56
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 26.6 bits (56), Expect = 3.7
Identities = 13/48 (27%), Positives = 25/48 (52%)
Frame = -1
Query: 330 TLHANSFLHARTIFFRSYSEERPCTESASYARFVAGYGRGLNHLERRP 187
T ANSF + + F+ + ++ S +F+ G+G+G+ L +P
Sbjct: 2795 TAGANSFYDSMSSGFKGLKKPFTDPKNNSAGKFLKGFGKGMLGLATKP 2842
>SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 455
Score = 26.2 bits (55), Expect = 4.9
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 6/62 (9%)
Frame = +1
Query: 253 LGARPFLGI*PEEDRAGMQEGVRVQGTVVEH-PEYGEVL-----QLQGDQRENICQWLTK 414
+ A P + RAG + V V G++VEH P + ++L +L GD + I + K
Sbjct: 374 MSAVPIASLYLSTGRAGKKSDVGVDGSLVEHYPHFVDMLREALRELIGDNEKLISIGIAK 433
Query: 415 SG 420
G
Sbjct: 434 DG 435
>SPCC663.14c |||TRP-like ion channel|Schizosaccharomyces pombe|chr
3|||Manual
Length = 687
Score = 25.4 bits (53), Expect = 8.6
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = +3
Query: 552 REHRRLQRKENTVVRALCLANSSVV*NIVNLIYYRRRL 665
R +R K T A C++N+ +V N + YY R L
Sbjct: 24 RYSKRPNHKLGTAEYAECMSNAPIVFNRFDASYYTRNL 61
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,947,603
Number of Sequences: 5004
Number of extensions: 59548
Number of successful extensions: 185
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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