Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40317
(701 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g20250.1 68416.m02565 pumilio/Puf RNA-binding domain-containi... 35 0.060
At3g16810.1 68416.m02147 pumilio/Puf RNA-binding domain-containi... 33 0.24
At3g10360.1 68416.m01242 pumilio/Puf RNA-binding domain-containi... 33 0.24
At2g29140.1 68415.m03542 pumilio/Puf RNA-binding domain-containi... 33 0.24
At2g29200.1 68415.m03549 pumilio/Puf RNA-binding domain-containi... 31 0.56
At1g69480.1 68414.m07983 EXS family protein / ERD1/XPR1/SYG1 fam... 30 1.3
At4g25880.2 68417.m03722 pumilio/Puf RNA-binding domain-containi... 30 1.7
At4g25880.1 68417.m03721 pumilio/Puf RNA-binding domain-containi... 30 1.7
At4g10120.1 68417.m01655 sucrose-phosphate synthase, putative si... 29 3.0
At5g49880.1 68418.m06177 mitotic checkpoint family protein simil... 29 3.9
At2g29190.1 68415.m03548 pumilio/Puf RNA-binding domain-containi... 29 3.9
At3g51650.1 68416.m05664 expressed protein 28 6.9
At3g51640.1 68416.m05663 expressed protein 28 6.9
At2g19670.1 68415.m02299 protein arginine N-methyltransferase, p... 28 6.9
At3g57830.1 68416.m06447 leucine-rich repeat transmembrane prote... 27 9.1
At1g21620.1 68414.m02706 pumilio/Puf RNA-binding domain-containi... 27 9.1
At1g01410.1 68414.m00056 hypothetical protein 27 9.1
>At3g20250.1 68416.m02565 pumilio/Puf RNA-binding domain-containing
protein contains Pfam profile: PF00806 Pumilio-family
RNA binding domains (aka PUM-HD, Pumilio homology
domain) (8 copies at C-terminus)
Length = 961
Score = 34.7 bits (76), Expect = 0.060
Identities = 18/72 (25%), Positives = 37/72 (51%)
Frame = +3
Query: 279 YAHHSVKRILKYGTDFIRHEVLKKFYGHVVSLSTHAISAPVLDYAYGEFASKKEKCHMQQ 458
+ ++ +++ +++GT R E++K+ G +VSLS V+ A E +K + +
Sbjct: 677 FGNYVIQKFIEHGTPAQREELVKQLAGQMVSLSLQMYGCRVIQKAL-EVIDVDQKTELIR 735
Query: 459 EFYGEIYKNTKD 494
E G + K +D
Sbjct: 736 ELDGNVLKCVRD 747
Score = 28.3 bits (60), Expect = 5.2
Identities = 12/41 (29%), Positives = 25/41 (60%)
Frame = +3
Query: 237 ERASRHIVQMGQSKYAHHSVKRILKYGTDFIRHEVLKKFYG 359
E+ + ++VQM Q KYA + V++ L++ R ++++ G
Sbjct: 844 EKLTGNVVQMSQHKYASNVVEKCLEHADSTEREFLIEEIMG 884
>At3g16810.1 68416.m02147 pumilio/Puf RNA-binding domain-containing
protein contains Pfam profile:PF00806 Pumilio-family RNA
binding domains
Length = 641
Score = 32.7 bits (71), Expect = 0.24
Identities = 29/134 (21%), Positives = 60/134 (44%), Gaps = 6/134 (4%)
Frame = +3
Query: 258 VQMGQSKYAHHSVKRILKYGTDFIRHEVLKKFYGHVVSLSTHAISAPVLDYAYGEFASKK 437
+ + +KYA H ++++L + + GHV L H + V+++AY S
Sbjct: 160 LNLASNKYAVHFIQKMLDGASKQQLAACISSLRGHVAPLLRHVFGSLVVEHAY-HLGSAA 218
Query: 438 EKCHMQQEFYG---EIYK--NTKDDKVKTSEMHTKTVQK*NRLYSRPVSKLTEDIRQKP- 599
+K + E Y +++K T ++K + +QK +R ++ + + I +K
Sbjct: 219 QKQELLAELYSTELQLFKGLTTSNEKTVVDIIAKLGLQK--GAVNRHMTAIIQPILEKGI 276
Query: 600 T*WRIVHSVLYDYI 641
I H +L +Y+
Sbjct: 277 VDHTITHKLLIEYL 290
Score = 28.7 bits (61), Expect = 3.9
Identities = 14/57 (24%), Positives = 30/57 (52%)
Frame = +1
Query: 34 FEISHKAKLLAAQIQRKVVKSDFRTNSCKELHNMLKGQYKSIALTHDLSRVIQVLLK 204
+ + + L +++R+ + + R+ E +KG+ IA++H SRV+Q +K
Sbjct: 85 YNLEQELVSLWEKMRRRNIGKEDRSKLISEAIRKMKGKVPEIAVSHVSSRVLQTCVK 141
>At3g10360.1 68416.m01242 pumilio/Puf RNA-binding domain-containing
protein similar to RNA binding protein PufA GB:AAD39751
[Dictyostelium discoideum] and similar to Pumilio
protein GB:A46221 [Drosophila sp.]
Length = 1003
Score = 32.7 bits (71), Expect = 0.24
Identities = 17/72 (23%), Positives = 36/72 (50%)
Frame = +3
Query: 279 YAHHSVKRILKYGTDFIRHEVLKKFYGHVVSLSTHAISAPVLDYAYGEFASKKEKCHMQQ 458
+ ++ +++ ++GT R E+ ++ GHV++LS V+ A E +++ M +
Sbjct: 713 FGNYVIQKFFEHGTTKQRKELAEQVTGHVLALSLQMYGCRVIQKAL-EVVELEQQARMVK 771
Query: 459 EFYGEIYKNTKD 494
E G + K D
Sbjct: 772 ELDGSVMKCVHD 783
>At2g29140.1 68415.m03542 pumilio/Puf RNA-binding domain-containing
protein
Length = 964
Score = 32.7 bits (71), Expect = 0.24
Identities = 16/81 (19%), Positives = 38/81 (46%)
Frame = +3
Query: 252 HIVQMGQSKYAHHSVKRILKYGTDFIRHEVLKKFYGHVVSLSTHAISAPVLDYAYGEFAS 431
H+++ + + +H V++ ++ + ++ F+GHVV+LSTH V+ +
Sbjct: 738 HVMRCVRDQNGNHVVQKCIECVPEENIEFIISTFFGHVVTLSTHPYGCRVIQRVLEHCHN 797
Query: 432 KKEKCHMQQEFYGEIYKNTKD 494
+ + +E + T+D
Sbjct: 798 PDTQSKVMEEILSTVSMLTQD 818
>At2g29200.1 68415.m03549 pumilio/Puf RNA-binding domain-containing
protein similar to BPM [Hordeum vulgare] GI:20513851
Length = 968
Score = 31.5 bits (68), Expect = 0.56
Identities = 13/51 (25%), Positives = 29/51 (56%)
Frame = +3
Query: 252 HIVQMGQSKYAHHSVKRILKYGTDFIRHEVLKKFYGHVVSLSTHAISAPVL 404
H+++ + + +H V++ ++ + ++ F+GHVV+LSTH V+
Sbjct: 742 HVMRCVRDQNGNHVVQKCIECVPEENIEFIISTFFGHVVTLSTHPYGCRVI 792
>At1g69480.1 68414.m07983 EXS family protein / ERD1/XPR1/SYG1 family
protein similar to PHO1 protein [Arabidopsis thaliana]
GI:20069032; contains Pfam profiles PF03105: SPX domain,
PF03124: EXS family
Length = 777
Score = 30.3 bits (65), Expect = 1.3
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = -1
Query: 152 LYCPFNILCNSLQEFVLKSDFTTFLCICA 66
L+CPFNI+ S + F ++S F CICA
Sbjct: 510 LFCPFNIIYRSSRFFFIRS---LFHCICA 535
>At4g25880.2 68417.m03722 pumilio/Puf RNA-binding domain-containing
protein contains Pfam profile:PF00806 Pumilio-family RNA
binding domains
Length = 852
Score = 29.9 bits (64), Expect = 1.7
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = +3
Query: 240 RASRHIVQMGQSKYAHHSVKRILKYGTDFIRHEVLKKFYG 359
+ S HIVQ+ K+A + +++ L+YG R ++K+ G
Sbjct: 740 KLSGHIVQLSLHKFASNVIEKCLEYGGRVERDLIIKEIAG 779
>At4g25880.1 68417.m03721 pumilio/Puf RNA-binding domain-containing
protein contains Pfam profile:PF00806 Pumilio-family RNA
binding domains
Length = 861
Score = 29.9 bits (64), Expect = 1.7
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = +3
Query: 240 RASRHIVQMGQSKYAHHSVKRILKYGTDFIRHEVLKKFYG 359
+ S HIVQ+ K+A + +++ L+YG R ++K+ G
Sbjct: 749 KLSGHIVQLSLHKFASNVIEKCLEYGGRVERDLIIKEIAG 788
>At4g10120.1 68417.m01655 sucrose-phosphate synthase, putative
similar to sucrose-phosphate synthase, Zea mays,
PIR2:JQ1329; contains non-consensus (GC) donor splice
site at intron 4
Length = 1050
Score = 29.1 bits (62), Expect = 3.0
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = +3
Query: 360 HVVSLSTHAISAPVLDYAYGE 422
H V L T IS+P +DY+YGE
Sbjct: 240 HRVDLLTRQISSPEVDYSYGE 260
>At5g49880.1 68418.m06177 mitotic checkpoint family protein similar
to mitotic checkpoint protein isoform MAD1a [Homo
sapiens] GI:4580767; contains Pfam profile PF05557:
Mitotic checkpoint protein
Length = 726
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/57 (22%), Positives = 29/57 (50%)
Frame = +1
Query: 4 RRKAKITAEVFEISHKAKLLAAQIQRKVVKSDFRTNSCKELHNMLKGQYKSIALTHD 174
R K ++ ++ ++ +AKL A+I + + R + + N+L+ Q + + L D
Sbjct: 192 REKKRLNNDIAQLGKEAKLSVARIGADLERMQCRAQNAETESNLLRSQLEHLKLIFD 248
>At2g29190.1 68415.m03548 pumilio/Puf RNA-binding domain-containing
protein
Length = 972
Score = 28.7 bits (61), Expect = 3.9
Identities = 12/51 (23%), Positives = 29/51 (56%)
Frame = +3
Query: 252 HIVQMGQSKYAHHSVKRILKYGTDFIRHEVLKKFYGHVVSLSTHAISAPVL 404
H+++ + + +H V++ ++ + ++ F+G+VV+LSTH V+
Sbjct: 746 HVMRCVRDQNGNHVVQKCIECVPEENIEFIISTFFGNVVTLSTHPYGCRVI 796
>At3g51650.1 68416.m05664 expressed protein
Length = 826
Score = 27.9 bits (59), Expect = 6.9
Identities = 17/52 (32%), Positives = 25/52 (48%), Gaps = 3/52 (5%)
Frame = +1
Query: 373 CQLMLLVHLSW---IMHMGSLLRKRKNATCNKNSMERFIRIQKMTR*RPQRC 519
C +LLV L W +M S R R+NA + I +QK+ + +RC
Sbjct: 34 CVFVLLVTLFWYEVLMPQLSTWRVRRNAQLRERERLEAIELQKLKKNATRRC 85
>At3g51640.1 68416.m05663 expressed protein
Length = 826
Score = 27.9 bits (59), Expect = 6.9
Identities = 17/52 (32%), Positives = 25/52 (48%), Gaps = 3/52 (5%)
Frame = +1
Query: 373 CQLMLLVHLSW---IMHMGSLLRKRKNATCNKNSMERFIRIQKMTR*RPQRC 519
C +LLV L W +M S R R+NA + I +QK+ + +RC
Sbjct: 34 CVFVLLVTLFWYEVLMPQLSTWRVRRNAQLRERERLEAIELQKLKKNATRRC 85
>At2g19670.1 68415.m02299 protein arginine N-methyltransferase,
putative similar to protein arginine N-methyltransferase
1-variant 1 [Homo sapiens] GI:7453577
Length = 366
Score = 27.9 bits (59), Expect = 6.9
Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = -1
Query: 359 SIKLLQNLM-PNEICSIFQYSLNGMMCVLRLTHLYYM 252
S+ + QN P ++ YSLNG C + TH Y M
Sbjct: 329 SMTIAQNKKNPRDVDIKLSYSLNGQHCNISRTHFYKM 365
>At3g57830.1 68416.m06447 leucine-rich repeat transmembrane protein
kinase, putative several receptor-like protein kinases
Length = 662
Score = 27.5 bits (58), Expect = 9.1
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = -3
Query: 552 IADFISGLSLYASLRSSPCHLLYSYKSLHRILVACG 445
I D+I SLY++L P + L S R+L+A G
Sbjct: 432 ITDYIRNGSLYSALHGGPSNTLPSLSWPERLLIAQG 467
>At1g21620.1 68414.m02706 pumilio/Puf RNA-binding domain-containing
protein contains Pfam profile: PF00806: Pumilio-family
RNA binding domains (aka PUM-HD, Pumilio homology
domain)
Length = 308
Score = 27.5 bits (58), Expect = 9.1
Identities = 15/63 (23%), Positives = 29/63 (46%)
Frame = +3
Query: 219 HYK*NNERASRHIVQMGQSKYAHHSVKRILKYGTDFIRHEVLKKFYGHVVSLSTHAISAP 398
HY + R +H + + Y + ++R+LK ++ ++ GH V LS +
Sbjct: 163 HYASHIAR-DKHALVLSNDAYGNFVIQRVLKLNDLRSKNNIVVSLRGHFVDLSFQKYGSY 221
Query: 399 VLD 407
V+D
Sbjct: 222 VVD 224
>At1g01410.1 68414.m00056 hypothetical protein
Length = 361
Score = 27.5 bits (58), Expect = 9.1
Identities = 12/54 (22%), Positives = 25/54 (46%)
Frame = +3
Query: 249 RHIVQMGQSKYAHHSVKRILKYGTDFIRHEVLKKFYGHVVSLSTHAISAPVLDY 410
RH++ + +K+++ DF++ E L H SLS H + ++ +
Sbjct: 31 RHLLVLSSDDNGCVILKKVITIADDFLKDEFLDLIAQHAHSLSMHDLGISLIQH 84
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,493,261
Number of Sequences: 28952
Number of extensions: 299444
Number of successful extensions: 770
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 737
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 770
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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