Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40309
(850 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI00015B6430 Cluster: PREDICTED: similar to conserved ... 94 5e-18
UniRef50_Q16TL0 Cluster: Putative uncharacterized protein; n=1; ... 75 2e-12
UniRef50_Q9W494 Cluster: CG12410-PA; n=2; Sophophora|Rep: CG1241... 69 1e-10
UniRef50_Q9GZX9 Cluster: Twisted gastrulation protein homolog 1 ... 59 2e-07
UniRef50_Q6IMD6 Cluster: Twisted gastrulation 2; n=5; Xenopus|Re... 54 5e-06
UniRef50_Q0PL68 Cluster: Twisted gastrulation; n=1; Saccoglossus... 53 8e-06
UniRef50_Q9VZE5 Cluster: CG11582-PA; n=2; Sophophora|Rep: CG1158... 51 4e-05
UniRef50_P54356 Cluster: Protein twisted gastrulation precursor;... 49 1e-04
UniRef50_UPI00005852C2 Cluster: PREDICTED: similar to twisted ga... 46 0.001
UniRef50_UPI00015B454A Cluster: PREDICTED: similar to conserved ... 44 0.004
UniRef50_UPI0000F2147D Cluster: PREDICTED: similar to Twsg1b pro... 36 0.97
UniRef50_Q4LE75 Cluster: CENPE variant protein; n=9; Euteleostom... 34 5.2
UniRef50_Q02224 Cluster: Centromeric protein E; n=8; Eutheria|Re... 34 5.2
UniRef50_Q0ZB79 Cluster: EIF2B-gamma protein; n=1; Bombyx mori|R... 33 6.9
UniRef50_A0E169 Cluster: Chromosome undetermined scaffold_72, wh... 33 6.9
UniRef50_UPI000069EC4E Cluster: GAS2-like protein 1 (Growth arre... 33 9.1
UniRef50_Q7REP8 Cluster: Putative uncharacterized protein PY0501... 33 9.1
>UniRef50_UPI00015B6430 Cluster: PREDICTED: similar to conserved
hypothetical protein; n=1; Nasonia vitripennis|Rep:
PREDICTED: similar to conserved hypothetical protein -
Nasonia vitripennis
Length = 377
Score = 93.9 bits (223), Expect = 5e-18
Identities = 41/101 (40%), Positives = 60/101 (59%), Gaps = 2/101 (1%)
Frame = +2
Query: 512 PGLFAALTSDPDAQQRWLSMTYPVDIDLSAYRPVPEKQVVYHLQSVEQ--DSEPVNTDMV 685
P LF AL +PD +RW S TYP+ + P EK Y++QS ++ +S+ V
Sbjct: 232 PELFRALNEEPDEHKRWNSFTYPISLPNRILSPQQEKNFEYYMQSADKKYNSKTVKLQAA 291
Query: 686 TFNCTVAYMSQCMSCDKCRASCRSMGPTALGGSMTGCCECV 808
T NCTV+++++C S KC+ SC+SMG T+ GCCEC+
Sbjct: 292 TTNCTVSFLAECNSWSKCKQSCQSMGATSYRWFHDGCCECI 332
Score = 57.2 bits (132), Expect = 5e-07
Identities = 39/113 (34%), Positives = 48/113 (42%), Gaps = 1/113 (0%)
Frame = +3
Query: 285 AYACNEAICASVVSKCMLTQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDMCPKPNDTQ 464
AY CNEAICAS VSKCML D+CPK NDTQ
Sbjct: 155 AYGCNEAICASTVSKCMLINSCKCDLTTCSCCKDCFLCLSLLYDECCSCFDLCPKSNDTQ 214
Query: 465 T-ELSKTSYVEELGDGYQASLLL*QVIQMLNNAGSL*HIQWTSTYQPTDLYQR 620
T LSK S+VE+ + + ++ + LN H +W S P L R
Sbjct: 215 TVPLSKKSHVEDFDEPFP------ELFRALNEEPDE-HKRWNSFTYPISLPNR 260
>UniRef50_Q16TL0 Cluster: Putative uncharacterized protein; n=1;
Aedes aegypti|Rep: Putative uncharacterized protein -
Aedes aegypti (Yellowfever mosquito)
Length = 159
Score = 74.9 bits (176), Expect = 2e-12
Identities = 36/81 (44%), Positives = 44/81 (54%)
Frame = +3
Query: 258 VALICVVPIAYACNEAICASVVSKCMLTQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVD 437
V LIC+ AY+CNE +CAS+VSKCMLTQ ++
Sbjct: 16 VGLICLAQFAYSCNEMVCASIVSKCMLTQSCKCDMKNCSCCKECSECLSYLYTECCSCLE 75
Query: 438 MCPKPNDTQTELSKTSYVEEL 500
MCPKPN+T ELSK S+VEEL
Sbjct: 76 MCPKPNETTNELSKQSHVEEL 96
Score = 40.7 bits (91), Expect = 0.045
Identities = 17/46 (36%), Positives = 27/46 (58%)
Frame = +2
Query: 512 PGLFAALTSDPDAQQRWLSMTYPVDIDLSAYRPVPEKQVVYHLQSV 649
P LF LTS+ D ++RW T+P+D+D + Y E + Y + S+
Sbjct: 100 PNLFNVLTSEEDPEERWRIFTFPIDLDAAMYGSNAETGMKYLMLSL 145
>UniRef50_Q9W494 Cluster: CG12410-PA; n=2; Sophophora|Rep:
CG12410-PA - Drosophila melanogaster (Fruit fly)
Length = 257
Score = 69.3 bits (162), Expect = 1e-10
Identities = 37/106 (34%), Positives = 56/106 (52%), Gaps = 6/106 (5%)
Frame = +2
Query: 509 VPGLFAALTSDPDAQQ---RWLSMTYPVDIDLSAYRPVPEKQVVYHLQSVEQDSEPV--- 670
VP LF A+ + + W T+ VD D P EK Y L++ +++ +
Sbjct: 98 VPELFNAVATPDEGDSFGYNWNVFTFQVDFDKYLKGPKLEKDGHYFLRTNDKNLDEAIQE 157
Query: 671 NTDMVTFNCTVAYMSQCMSCDKCRASCRSMGPTALGGSMTGCCECV 808
++VT NCTV Y+ QC+S +KCR SC++ G ++ GCCECV
Sbjct: 158 RDNIVTVNCTVIYLDQCVSWNKCRTSCQTTGASSTRWFHDGCCECV 203
Score = 66.5 bits (155), Expect = 8e-10
Identities = 32/87 (36%), Positives = 45/87 (51%)
Frame = +3
Query: 249 LFIVALICVVPIAYACNEAICASVVSKCMLTQXXXXXXXXXXXXXXXXXXXXXXXXXXXX 428
+ ++A++C +CNE +CAS+VSKCMLTQ
Sbjct: 12 IVLLAIVCFYGTVESCNEVVCASIVSKCMLTQSCKCELKNCSCCKECLKCLGKNYEECCS 71
Query: 429 XVDMCPKPNDTQTELSKTSYVEELGDG 509
V++CPKPNDT+ LSK S+VE+ DG
Sbjct: 72 CVELCPKPNDTRNSLSKKSHVEDF-DG 97
>UniRef50_Q9GZX9 Cluster: Twisted gastrulation protein homolog 1
precursor; n=23; Euteleostomi|Rep: Twisted gastrulation
protein homolog 1 precursor - Homo sapiens (Human)
Length = 223
Score = 58.8 bits (136), Expect = 2e-07
Identities = 36/106 (33%), Positives = 51/106 (48%), Gaps = 6/106 (5%)
Frame = +2
Query: 509 VPGLFAALTSDPDAQQRWLSMTYPVDIDLSAYRPVP---EKQVVYHLQSVEQDSEPVNTD 679
+P LF ALT + D Q W +++PV +LS + + E H Q+V S V+
Sbjct: 100 IPSLFRALT-EGDTQLNWNIVSFPVAEELSHHENLVSFLETVNQPHHQNVSVPSNNVHAP 158
Query: 680 MVTFN---CTVAYMSQCMSCDKCRASCRSMGPTALGGSMTGCCECV 808
+ CTV Y CMS +C+ SC SMG + CCEC+
Sbjct: 159 YSSDKEHMCTVVYFDDCMSIHQCKISCESMGASKYRWFHNACCECI 204
Score = 33.5 bits (73), Expect = 6.9
Identities = 13/32 (40%), Positives = 23/32 (71%)
Frame = +3
Query: 249 LFIVALICVVPIAYACNEAICASVVSKCMLTQ 344
L I+ + +P + +CN+A+CAS VSKC++ +
Sbjct: 11 LAILMFLTWLPESLSCNKALCASDVSKCLIQE 42
>UniRef50_Q6IMD6 Cluster: Twisted gastrulation 2; n=5; Xenopus|Rep:
Twisted gastrulation 2 - Xenopus laevis (African clawed
frog)
Length = 248
Score = 54.0 bits (124), Expect = 5e-06
Identities = 33/102 (32%), Positives = 48/102 (47%), Gaps = 2/102 (1%)
Frame = +2
Query: 509 VPGLFAALTSDPDAQQR--WLSMTYPVDIDLSAYRPVPEKQVVYHLQSVEQDSEPVNTDM 682
VP LF A++S + + W S + P+ +L+ + ++ SV S NT
Sbjct: 119 VPSLFRAVSSMQEGESALGWTSHSLPIREELA--QSTHMDHILLGAHSVVTASPLTNTSD 176
Query: 683 VTFNCTVAYMSQCMSCDKCRASCRSMGPTALGGSMTGCCECV 808
CTV Y + CMS +C SC S+G T GCC+CV
Sbjct: 177 F---CTVLYFNSCMSMRRCHQSCESVGSTKYRWFHNGCCQCV 215
Score = 40.7 bits (91), Expect = 0.045
Identities = 19/38 (50%), Positives = 26/38 (68%), Gaps = 1/38 (2%)
Frame = +3
Query: 234 HVEKVLFIVALIC-VVPIAYACNEAICASVVSKCMLTQ 344
H +LFI AL+C V + CN+A+CAS VSKC+L +
Sbjct: 24 HPGVLLFISALLCSTVTLVTGCNKALCASDVSKCLLQE 61
>UniRef50_Q0PL68 Cluster: Twisted gastrulation; n=1; Saccoglossus
kowalevskii|Rep: Twisted gastrulation - Saccoglossus
kowalevskii (Acorn worm)
Length = 205
Score = 53.2 bits (122), Expect = 8e-06
Identities = 30/107 (28%), Positives = 49/107 (45%), Gaps = 2/107 (1%)
Frame = +2
Query: 509 VPGLFAALTSDP--DAQQRWLSMTYPVDIDLSAYRPVPEKQVVYHLQSVEQDSEPVNTDM 682
+P LF ALT D D +W + + ++ +Y + +D +
Sbjct: 98 IPSLFDALTEDGYIDYDSQWSVVKFTHLVE-----------TLYSFKPPNKDEDKALNKT 146
Query: 683 VTFNCTVAYMSQCMSCDKCRASCRSMGPTALGGSMTGCCECVRRQVL 823
+ CTV Y+ +C+S DKC +C+SMG +A + CCEC+ L
Sbjct: 147 LGKECTVVYLHECISMDKCSLACQSMGASAYRWFHSKCCECIGHMCL 193
Score = 39.1 bits (87), Expect = 0.14
Identities = 23/88 (26%), Positives = 36/88 (40%)
Frame = +3
Query: 237 VEKVLFIVALICVVPIAYACNEAICASVVSKCMLTQXXXXXXXXXXXXXXXXXXXXXXXX 416
+ +LF +++ + + +ACNEA+CAS VSKC L +
Sbjct: 6 IRVLLFTLSVCYAIYLVHACNEAMCASDVSKCQLMESCSCNFKNHSCSQNCSRCLGPLYT 65
Query: 417 XXXXXVDMCPKPNDTQTELSKTSYVEEL 500
V MC N + + S VE+L
Sbjct: 66 ECCDCVGMCKPRNFSHKPYAAKSIVEDL 93
>UniRef50_Q9VZE5 Cluster: CG11582-PA; n=2; Sophophora|Rep:
CG11582-PA - Drosophila melanogaster (Fruit fly)
Length = 116
Score = 50.8 bits (116), Expect = 4e-05
Identities = 20/39 (51%), Positives = 24/39 (61%)
Frame = +2
Query: 692 NCTVAYMSQCMSCDKCRASCRSMGPTALGGSMTGCCECV 808
NCTV Y+ QC S +KCR +C G T+ GCCECV
Sbjct: 51 NCTVLYLDQCTSWNKCRQTCLKTGATSYRWFHDGCCECV 89
>UniRef50_P54356 Cluster: Protein twisted gastrulation precursor;
n=2; Sophophora|Rep: Protein twisted gastrulation
precursor - Drosophila melanogaster (Fruit fly)
Length = 249
Score = 49.2 bits (112), Expect = 1e-04
Identities = 19/43 (44%), Positives = 25/43 (58%)
Frame = +2
Query: 695 CTVAYMSQCMSCDKCRASCRSMGPTALGGSMTGCCECVRRQVL 823
CTV Y++ C+ +KCR C SMG ++ GCCECV L
Sbjct: 152 CTVIYVNSCIRANKCRQQCESMGASSYRWFHDGCCECVGENCL 194
Score = 41.1 bits (92), Expect = 0.034
Identities = 23/71 (32%), Positives = 29/71 (40%)
Frame = +3
Query: 294 CNEAICASVVSKCMLTQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDMCPKPNDTQTEL 473
CNE +C SVVSKC++TQ +DMCPK D L
Sbjct: 26 CNEVVCGSVVSKCLITQSCQCKLNDCHCCKDCLNCLGELYIECCGCLDMCPKHKDVLPSL 85
Query: 474 SKTSYVEELGD 506
+ S E+GD
Sbjct: 86 TPRS---EIGD 93
>UniRef50_UPI00005852C2 Cluster: PREDICTED: similar to twisted
gastrulation; n=1; Strongylocentrotus purpuratus|Rep:
PREDICTED: similar to twisted gastrulation -
Strongylocentrotus purpuratus
Length = 222
Score = 46.4 bits (105), Expect = 0.001
Identities = 20/48 (41%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = +2
Query: 668 VNTDMVTFN-CTVAYMSQCMSCDKCRASCRSMGPTALGGSMTGCCECV 808
VN +V + CTVAY+ C+ +KC +C MG TGCCEC+
Sbjct: 155 VNQTIVPIDICTVAYIKICLGLEKCVDACHHMGAAKYRWFHTGCCECI 202
>UniRef50_UPI00015B454A Cluster: PREDICTED: similar to conserved
hypothetical protein; n=1; Nasonia vitripennis|Rep:
PREDICTED: similar to conserved hypothetical protein -
Nasonia vitripennis
Length = 229
Score = 44.4 bits (100), Expect = 0.004
Identities = 24/76 (31%), Positives = 32/76 (42%), Gaps = 1/76 (1%)
Frame = +3
Query: 288 YACNEAICASVVSKCMLTQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDMCPKPNDTQT 467
Y CNEA+CAS VSKCML ++CP N T+T
Sbjct: 50 YGCNEAVCASTVSKCMLLNSCKCDLSTCSCCKDCFLCLSFLFDEYCSCFNLCPNSNVTET 109
Query: 468 -ELSKTSYVEELGDGY 512
L K S+VE+ + +
Sbjct: 110 ISLGKKSHVEDFDEPF 125
Score = 37.9 bits (84), Expect(2) = 0.023
Identities = 15/49 (30%), Positives = 24/49 (48%)
Frame = +2
Query: 662 EPVNTDMVTFNCTVAYMSQCMSCDKCRASCRSMGPTALGGSMTGCCECV 808
EP V++++ C KC+ SC+S+ T+ GCCEC+
Sbjct: 135 EPDEHKRQELQTAVSFLTDCHPWSKCKQSCQSIRATSYRWFHDGCCECI 183
Score = 23.0 bits (47), Expect(2) = 0.023
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +2
Query: 512 PGLFAALTSDPDAQQR 559
P LF ALT +PD +R
Sbjct: 126 PELFRALTQEPDEHKR 141
>UniRef50_UPI0000F2147D Cluster: PREDICTED: similar to Twsg1b
protein, partial; n=1; Danio rerio|Rep: PREDICTED:
similar to Twsg1b protein, partial - Danio rerio
Length = 261
Score = 36.3 bits (80), Expect = 0.97
Identities = 14/30 (46%), Positives = 23/30 (76%)
Frame = +3
Query: 255 IVALICVVPIAYACNEAICASVVSKCMLTQ 344
++ L+ + IA ACN+A+CAS VSKC++ +
Sbjct: 200 VLLLLSSLSIALACNKALCASDVSKCLIQE 229
>UniRef50_Q4LE75 Cluster: CENPE variant protein; n=9;
Euteleostomi|Rep: CENPE variant protein - Homo sapiens
(Human)
Length = 2585
Score = 33.9 bits (74), Expect = 5.2
Identities = 16/30 (53%), Positives = 23/30 (76%), Gaps = 1/30 (3%)
Frame = -1
Query: 406 ELRQLKQSLQHEQS-FKSHLHDWVNMHFDT 320
+L+QL++SLQ E+ KS +HD VNM+ DT
Sbjct: 913 DLKQLQESLQIERDQLKSDIHDTVNMNIDT 942
>UniRef50_Q02224 Cluster: Centromeric protein E; n=8; Eutheria|Rep:
Centromeric protein E - Homo sapiens (Human)
Length = 2663
Score = 33.9 bits (74), Expect = 5.2
Identities = 16/30 (53%), Positives = 23/30 (76%), Gaps = 1/30 (3%)
Frame = -1
Query: 406 ELRQLKQSLQHEQS-FKSHLHDWVNMHFDT 320
+L+QL++SLQ E+ KS +HD VNM+ DT
Sbjct: 933 DLKQLQESLQIERDQLKSDIHDTVNMNIDT 962
>UniRef50_Q0ZB79 Cluster: EIF2B-gamma protein; n=1; Bombyx mori|Rep:
EIF2B-gamma protein - Bombyx mori (Silk moth)
Length = 459
Score = 33.5 bits (73), Expect = 6.9
Identities = 20/66 (30%), Positives = 34/66 (51%), Gaps = 6/66 (9%)
Frame = -1
Query: 391 KQSLQHEQSFKSHLHDWVNMHFDTTL---AHIASLHAYAIGTTQISA---TINKTFSTWQ 230
++ ++ ++ H H ++F+ TL AHI S + +AI +S+ + NK S WQ
Sbjct: 276 ERKIREISAYNDHKHGNKGVYFNDTLRCYAHIPSKNTFAIRVNTLSSFYLSNNKVLSKWQ 335
Query: 229 DLMKQS 212
DL S
Sbjct: 336 DLTGSS 341
>UniRef50_A0E169 Cluster: Chromosome undetermined scaffold_72, whole
genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_72,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 653
Score = 33.5 bits (73), Expect = 6.9
Identities = 15/40 (37%), Positives = 27/40 (67%)
Frame = -1
Query: 433 TQLQHSLYKELRQLKQSLQHEQSFKSHLHDWVNMHFDTTL 314
TQLQ+SL+K+L +L Q+ ++ ++ + LH W+N D +
Sbjct: 241 TQLQYSLFKKLTELDQTKRY-RADQGLLHPWLNEQIDVEI 279
>UniRef50_UPI000069EC4E Cluster: GAS2-like protein 1 (Growth
arrest-specific 2-like 1) (GAS2-related protein on
chromosome 22) (GAR22 protein).; n=1; Xenopus
tropicalis|Rep: GAS2-like protein 1 (Growth
arrest-specific 2-like 1) (GAS2-related protein on
chromosome 22) (GAR22 protein). - Xenopus tropicalis
Length = 632
Score = 33.1 bits (72), Expect = 9.1
Identities = 13/37 (35%), Positives = 23/37 (62%)
Frame = -1
Query: 433 TQLQHSLYKELRQLKQSLQHEQSFKSHLHDWVNMHFD 323
+Q+Q S K +R + S ++ ++ K L DW+NM +D
Sbjct: 5 SQIQSSASKSIRPFRSSEEYLEAMKEDLTDWLNMLYD 41
>UniRef50_Q7REP8 Cluster: Putative uncharacterized protein PY05015;
n=4; Plasmodium (Vinckeia)|Rep: Putative uncharacterized
protein PY05015 - Plasmodium yoelii yoelii
Length = 644
Score = 33.1 bits (72), Expect = 9.1
Identities = 27/96 (28%), Positives = 49/96 (51%), Gaps = 7/96 (7%)
Frame = -3
Query: 320 YACTYSFIACVCYWHHTNQRDDK*DLLHMARFNETIKECLKNKQ----RSNFKDVN--SC 159
Y C+Y + + Y+ +DDK ++ + N IK L NKQ + FK+ N
Sbjct: 246 YICSYKYNIILPYYQDIQNKDDKINI----KNNNQIKTKLTNKQIIQLKKQFKNKNIFKI 301
Query: 158 AFVY*ILSHVVIINL*CFAF-LKTDIREVLITSKIS 54
++ I+S +VI++ F++ L+ ++ LI S+ S
Sbjct: 302 IMLFIIISIIVILSTCLFSYILEISQKKFLINSENS 337
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 800,206,658
Number of Sequences: 1657284
Number of extensions: 15374069
Number of successful extensions: 44013
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 41708
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 43965
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 74603367202
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -