Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40294
(758 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 127 1e-30
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 124 2e-29
SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces pomb... 29 0.72
SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces p... 28 1.7
SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 27 2.2
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 27 2.9
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 27 2.9
SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces pom... 27 2.9
SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 27 3.8
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 26 5.1
SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces p... 26 5.1
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 26 5.1
SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch... 26 6.7
SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 26 6.7
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 26 6.7
SPAC22H10.10 |alp21|sto1|tubulin specific chaperone cofactor E|S... 26 6.7
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 25 8.9
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 127 bits (307), Expect = 1e-30
Identities = 58/88 (65%), Positives = 73/88 (82%), Gaps = 2/88 (2%)
Frame = +2
Query: 254 SYKNVVGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKH 427
+YKN++GARR+SWR+ISSIEQK E G+ R+ + KEYR K+E EL +IC+DVL +L+KH
Sbjct: 49 AYKNIIGARRASWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKH 108
Query: 428 LIPKASNPESKVFYLKMKGDYYRYLAEW 511
LIP A+ ESKVFY KMKGDYYRYLAE+
Sbjct: 109 LIPAATTGESKVFYYKMKGDYYRYLAEF 136
Score = 107 bits (256), Expect = 2e-24
Identities = 49/80 (61%), Positives = 64/80 (80%)
Frame = +1
Query: 517 GETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQ 696
GE + S +AY+ A +I+ A++ PT P+RLGLALNFSVFYYEIL+SP+ AC LAKQ
Sbjct: 139 GEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQ 198
Query: 697 AFDDAIAELDTLNEDSYKDS 756
FD+AI+ELD+L+E+SYKDS
Sbjct: 199 VFDEAISELDSLSEESYKDS 218
Score = 51.6 bits (118), Expect = 1e-07
Identities = 28/48 (58%), Positives = 34/48 (70%)
Frame = +3
Query: 111 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSV 254
MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSV
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSV 48
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 124 bits (298), Expect = 2e-29
Identities = 56/88 (63%), Positives = 73/88 (82%), Gaps = 2/88 (2%)
Frame = +2
Query: 254 SYKNVVGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKH 427
+YKNV+GARR+SWR++SSIEQK E G+ + ++ KEYR K+E+EL IC D+L +L+KH
Sbjct: 50 AYKNVIGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKH 109
Query: 428 LIPKASNPESKVFYLKMKGDYYRYLAEW 511
LIP A++ ESKVFY KMKGDYYRYLAE+
Sbjct: 110 LIPNAASAESKVFYYKMKGDYYRYLAEF 137
Score = 121 bits (291), Expect = 1e-28
Identities = 56/82 (68%), Positives = 68/82 (82%)
Frame = +1
Query: 511 ATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLA 690
A GE R + S + Y+ A EI+ A++ PTHPIRLGLALNFSVFYYEILNSPD+AC LA
Sbjct: 138 AVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLA 197
Query: 691 KQAFDDAIAELDTLNEDSYKDS 756
KQAFD+AI+ELD+L+E+SYKDS
Sbjct: 198 KQAFDEAISELDSLSEESYKDS 219
Score = 52.8 bits (121), Expect = 5e-08
Identities = 27/47 (57%), Positives = 32/47 (68%)
Frame = +3
Query: 114 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSV 254
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSV
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSV 49
>SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 605
Score = 29.1 bits (62), Expect = 0.72
Identities = 16/38 (42%), Positives = 22/38 (57%)
Frame = -1
Query: 572 KASWYAFCESSTTECLVSPVAILQGTCSNLLSF*GKTL 459
+A W AF E+S +E ++ IL S+LLSF TL
Sbjct: 64 EAVWAAFSEASCSERKLALQGILNNCSSSLLSFASSTL 101
>SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 517
Score = 27.9 bits (59), Expect = 1.7
Identities = 9/41 (21%), Positives = 21/41 (51%)
Frame = -2
Query: 739 LRSMCPVRRWHRRKPVWRVDTPCLANLISHNKRLRNLTPDP 617
LR + P+ +W R++ +W + P + + ++ + P P
Sbjct: 119 LRDVTPINQWKRKRSLWDIKPPGYELVTADQAKMSGVFPLP 159
>SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 844
Score = 27.5 bits (58), Expect = 2.2
Identities = 19/47 (40%), Positives = 26/47 (55%), Gaps = 3/47 (6%)
Frame = +2
Query: 404 VLGLLDKHLIPKASN-PESKVFYLKMKGD--YYRYLAEWPQEKPDIL 535
VL L+ L+ A+ PE K+FY K GD Y +YL P + +IL
Sbjct: 509 VLMQLESFLLNIANPAPEGKLFYEKQTGDNPYLKYLLLDPSKHVEIL 555
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1207
Score = 27.1 bits (57), Expect = 2.9
Identities = 17/54 (31%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
Frame = +1
Query: 481 RRLLQVPCRMATGETRHSVVEDSQKAYQDAFEISKAKMQPTHP-IRLGLALNFS 639
R ++ + C + E R ED+++A + ++ + A +QP H I L LA+N S
Sbjct: 1049 RYIVALSCEL---ERRRLGEEDTKRALELSYYFASADLQPMHSIIALRLAINAS 1099
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 27.1 bits (57), Expect = 2.9
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +2
Query: 461 VFYLKMKGDYYRYLAEW 511
VF+ K DYYRY+ +W
Sbjct: 2160 VFFQNCKLDYYRYVGDW 2176
>SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 601
Score = 27.1 bits (57), Expect = 2.9
Identities = 26/91 (28%), Positives = 40/91 (43%), Gaps = 9/91 (9%)
Frame = +2
Query: 293 RVISSIEQKTEGSERKQQMAKEYRVKVEKE-LREICYDVLGL--LDKHL---IPKASNPE 454
+V+SS + E S + + R E E L E+ +V L +D+ +P+ NP
Sbjct: 263 KVLSSSSEDDESSSPEDLLKPIIRSTEEMENLNELEQEVQDLDPIDEGFEEKVPRFRNPS 322
Query: 455 SKVFYLK---MKGDYYRYLAEWPQEKPDILL 538
K FY K ++ Y L EK D L+
Sbjct: 323 KKAFYEKLHSLRNRSYSKLESLTSEKSDTLI 353
>SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl
cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 463
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +2
Query: 260 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVE 376
KN+ S+ R +SS + K +E + M +Y K+E
Sbjct: 350 KNLENDEESTLRALSSFQSKIRNAEDEDVMDSQYGSKIE 388
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.2 bits (55), Expect = 5.1
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +2
Query: 314 QKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 439
+K + + KEY K +++LR+ D+ LL KH I +
Sbjct: 328 EKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369
>SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 26.2 bits (55), Expect = 5.1
Identities = 21/68 (30%), Positives = 36/68 (52%), Gaps = 2/68 (2%)
Frame = +2
Query: 311 EQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM-KGD 487
E E+ Q++A+ Y+V + +LRE YD LG + +P A ++ F+ + GD
Sbjct: 43 ENPEAAREKFQKLAEAYQVLSDPKLRE-KYDKLGKVG--AVPDAGFEDAFEFFKNLFGGD 99
Query: 488 YYR-YLAE 508
+R Y+ E
Sbjct: 100 SFRDYVGE 107
>SPBC211.03c |||guanyl-nucleotide exchange
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1462
Score = 26.2 bits (55), Expect = 5.1
Identities = 12/32 (37%), Positives = 14/32 (43%)
Frame = -1
Query: 143 TLHQFFLVDGHRGRWKRTNSLMPELLKPAVLE 48
TL FL DG RWK + P V+E
Sbjct: 52 TLKDEFLADGLESRWKTNGEKSSSIRYPLVME 83
>SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 487
Score = 25.8 bits (54), Expect = 6.7
Identities = 12/43 (27%), Positives = 21/43 (48%)
Frame = +2
Query: 254 SYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKE 382
SY + G S W+ I ++ K+ G +R ++ Y +KE
Sbjct: 63 SYPFLKGKSDSPWQAIQLLDFKSSGQQRAAYYSERYHSFRDKE 105
>SPAC16A10.08c |mug74|SPAC589.01c|sequence
orphan|Schizosaccharomyces pombe|chr 1|||Manual
Length = 285
Score = 25.8 bits (54), Expect = 6.7
Identities = 14/51 (27%), Positives = 23/51 (45%)
Frame = +1
Query: 22 EIKKK*CLHSKTAGFNNSGISELVLFHRPRCPSTRKNWCNVPNWPNKLSDM 174
++K+ + S F N S+ + F +PR K N WPN+ S +
Sbjct: 50 QLKELDLVVSSNKEFLNEKTSDQISFLKPRETVVEKKLANGSIWPNETSHL 100
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 25.8 bits (54), Expect = 6.7
Identities = 14/59 (23%), Positives = 30/59 (50%)
Frame = +2
Query: 248 FSSYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDK 424
FSS +N +GA+R+ + ++ Q+ K+ + + +++E+ + LLDK
Sbjct: 2 FSSKENSLGAKRAPFSSNTTSSQRVAAQAAKRASSGAFAQLTSSQIQEL-KEAFALLDK 59
>SPAC22H10.10 |alp21|sto1|tubulin specific chaperone cofactor
E|Schizosaccharomyces pombe|chr 1|||Manual
Length = 511
Score = 25.8 bits (54), Expect = 6.7
Identities = 24/86 (27%), Positives = 35/86 (40%), Gaps = 1/86 (1%)
Frame = -2
Query: 670 LANLISHNKRLRNLTPDPALWGVWAAFSPC*FQKHLGMLSVNPLQQNVWFLLWPFCKVPV 491
+ L S LRNLT D L+ + + + HL LSVN N + W P
Sbjct: 159 ILKLCSQLPSLRNLTLDSNLFSNFISSNTVLLIPHLTQLSVNGCGLNSKDVQWITETFPS 218
Query: 490 VISFHFEVKHFTF-WITSFRN*VFVK 416
+ + E TSF+N F++
Sbjct: 219 LEVLYLEANEIILSKATSFKNLQFLQ 244
>SPBC6B1.04 |mde4||monopolin-like complex subunit
Mde4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 421
Score = 25.4 bits (53), Expect = 8.9
Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +2
Query: 332 ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNPE 454
E++Q A +YR+KVE+ +I V + L+ L + SNPE
Sbjct: 85 EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPE 127
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,185,591
Number of Sequences: 5004
Number of extensions: 66291
Number of successful extensions: 233
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 224
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 231
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -