Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40280
(623 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 132 2e-31
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 105 2e-23
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 105 2e-23
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 103 1e-22
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 71 8e-13
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 43 1e-04
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 43 1e-04
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 40 0.001
At5g13650.2 68418.m01585 elongation factor family protein contai... 33 0.12
At5g13650.1 68418.m01584 elongation factor family protein contai... 33 0.12
At2g03500.1 68415.m00309 myb family transcription factor contain... 31 0.47
At2g25730.1 68415.m03084 expressed protein 30 1.4
At5g03415.1 68418.m00294 DPB-1 transcription factor, putative (D... 28 5.8
At5g07990.1 68418.m00930 flavonoid 3'-monooxygenase / flavonoid ... 27 7.7
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 7.7
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 132 bits (319), Expect = 2e-31
Identities = 58/85 (68%), Positives = 72/85 (84%)
Frame = +1
Query: 256 IHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEE 435
IHRGGGQ+IPT RR +YA +TA+PRL+EPVY+ EIQ PE A+GGIY VLN++RGHVFEE
Sbjct: 699 IHRGGGQVIPTARRVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEE 758
Query: 436 SQVAGTPMFIVKAYLPVNESFGFTA 510
Q GTP++ +KAYLPV ESFGF++
Sbjct: 759 MQRPGTPLYNIKAYLPVVESFGFSS 783
Score = 125 bits (301), Expect = 3e-29
Identities = 55/85 (64%), Positives = 69/85 (81%)
Frame = +2
Query: 2 KTRARYLTEKYEYDVTEARKIWCFGPEGTGPNILVDCSKGVQYLNEIKDSVVAGFQWAAK 181
K R++ L E++ +D A+KIW FGPE TGPN++VD KGVQYLNEIKDSVVAGFQWA+K
Sbjct: 614 KIRSKILAEEFGWDKDLAKKIWAFGPETTGPNMVVDMCKGVQYLNEIKDSVVAGFQWASK 673
Query: 182 EGVMAEENLRGVRFNIYDVTLHTDA 256
EG +AEEN+RG+ F + DV LH+DA
Sbjct: 674 EGPLAEENMRGICFEVCDVVLHSDA 698
Score = 54.4 bits (125), Expect = 6e-08
Identities = 21/39 (53%), Positives = 28/39 (71%)
Frame = +3
Query: 501 FYCDLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPTTL 617
F LR+ T GQAFPQCVFDHW+++ DP EP ++ + L
Sbjct: 781 FSSQLRAATSGQAFPQCVFDHWEMMSSDPLEPGTQASVL 819
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 105 bits (253), Expect = 2e-23
Identities = 51/83 (61%), Positives = 58/83 (69%)
Frame = +1
Query: 256 IHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEE 435
+HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV +
Sbjct: 815 LHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSD 874
Query: 436 SQVAGTPMFIVKAYLPVNESFGF 504
GTP +IVKA+LPV ESFGF
Sbjct: 875 VPQPGTPAYIVKAFLPVIESFGF 897
Score = 75.8 bits (178), Expect = 2e-14
Identities = 33/80 (41%), Positives = 50/80 (62%), Gaps = 4/80 (5%)
Frame = +2
Query: 17 YLTEKYEYDVTEARKIWCFGPEGTGPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKE 184
+ KY++D+ AR IW FGP+ GPNIL+D + + + +KDS+V GFQW A+E
Sbjct: 731 FFRTKYDWDLLAARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGARE 790
Query: 185 GVMAEENLRGVRFNIYDVTL 244
G + +E +R V+F I D +
Sbjct: 791 GPLCDEPIRNVKFKIVDARI 810
Score = 44.4 bits (100), Expect = 6e-05
Identities = 18/31 (58%), Positives = 22/31 (70%)
Frame = +3
Query: 501 FYCDLRSNTGGQAFPQCVFDHWQVLPGDPCE 593
F DLR +T GQAF VFDHW ++PGDP +
Sbjct: 897 FETDLRYHTQGQAFCLSVFDHWAIVPGDPLD 927
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 105 bits (253), Expect = 2e-23
Identities = 51/83 (61%), Positives = 58/83 (69%)
Frame = +1
Query: 256 IHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEE 435
+HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV +
Sbjct: 815 LHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSD 874
Query: 436 SQVAGTPMFIVKAYLPVNESFGF 504
GTP +IVKA+LPV ESFGF
Sbjct: 875 VPQPGTPAYIVKAFLPVIESFGF 897
Score = 75.8 bits (178), Expect = 2e-14
Identities = 33/80 (41%), Positives = 50/80 (62%), Gaps = 4/80 (5%)
Frame = +2
Query: 17 YLTEKYEYDVTEARKIWCFGPEGTGPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKE 184
+ KY++D+ AR IW FGP+ GPNIL+D + + + +KDS+V GFQW A+E
Sbjct: 731 FFRTKYDWDLLAARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGARE 790
Query: 185 GVMAEENLRGVRFNIYDVTL 244
G + +E +R V+F I D +
Sbjct: 791 GPLCDEPIRNVKFKIVDARI 810
Score = 44.4 bits (100), Expect = 6e-05
Identities = 18/31 (58%), Positives = 22/31 (70%)
Frame = +3
Query: 501 FYCDLRSNTGGQAFPQCVFDHWQVLPGDPCE 593
F DLR +T GQAF VFDHW ++PGDP +
Sbjct: 897 FETDLRYHTQGQAFCLSVFDHWAIVPGDPLD 927
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 103 bits (247), Expect = 1e-22
Identities = 50/83 (60%), Positives = 58/83 (69%)
Frame = +1
Query: 256 IHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEE 435
+HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRG+V +
Sbjct: 801 LHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSD 860
Query: 436 SQVAGTPMFIVKAYLPVNESFGF 504
GTP +IVKA+LPV ESFGF
Sbjct: 861 VPQPGTPAYIVKAFLPVIESFGF 883
Score = 72.1 bits (169), Expect = 3e-13
Identities = 32/80 (40%), Positives = 49/80 (61%), Gaps = 4/80 (5%)
Frame = +2
Query: 17 YLTEKYEYDVTEARKIWCFGPEGTGPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKE 184
+ KY++D+ AR IW FGP+ G NIL+D + + + +KDS+V GFQW A+E
Sbjct: 717 FFRTKYDWDLLAARSIWAFGPDKQGTNILLDDTLPTEVDRNLMMGVKDSIVQGFQWGARE 776
Query: 185 GVMAEENLRGVRFNIYDVTL 244
G + +E +R V+F I D +
Sbjct: 777 GPLCDEPIRNVKFKIVDARI 796
Score = 44.4 bits (100), Expect = 6e-05
Identities = 18/31 (58%), Positives = 22/31 (70%)
Frame = +3
Query: 501 FYCDLRSNTGGQAFPQCVFDHWQVLPGDPCE 593
F DLR +T GQAF VFDHW ++PGDP +
Sbjct: 883 FETDLRYHTQGQAFCLSVFDHWAIVPGDPLD 913
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 70.5 bits (165), Expect = 8e-13
Identities = 30/78 (38%), Positives = 47/78 (60%)
Frame = +1
Query: 271 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAG 450
GQ++ + A +L PR++E +Y CE+ +G +Y VL+RRR + +E G
Sbjct: 853 GQVMTAVKDACRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEG 912
Query: 451 TPMFIVKAYLPVNESFGF 504
+ +F V AY+PV+ESFGF
Sbjct: 913 SSLFTVHAYVPVSESFGF 930
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 43.2 bits (97), Expect = 1e-04
Identities = 20/66 (30%), Positives = 36/66 (54%)
Frame = +1
Query: 310 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 489
C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706
Query: 490 ESFGFT 507
FG++
Sbjct: 707 NMFGYS 712
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 43.2 bits (97), Expect = 1e-04
Identities = 20/66 (30%), Positives = 36/66 (54%)
Frame = +1
Query: 310 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 489
C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706
Query: 490 ESFGFT 507
FG++
Sbjct: 707 NMFGYS 712
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 39.9 bits (89), Expect = 0.001
Identities = 20/63 (31%), Positives = 34/63 (53%)
Frame = +1
Query: 322 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 501
A PR++EP+ E+ PE +G + G LN RRG + G + +V + +P+ E F
Sbjct: 683 AGPRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQ 741
Query: 502 FTA 510
+ +
Sbjct: 742 YVS 744
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 33.5 bits (73), Expect = 0.12
Identities = 14/57 (24%), Positives = 28/57 (49%)
Frame = +1
Query: 331 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 501
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLG 529
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 33.5 bits (73), Expect = 0.12
Identities = 14/57 (24%), Positives = 28/57 (49%)
Frame = +1
Query: 331 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 501
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLG 528
>At2g03500.1 68415.m00309 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 432
Score = 31.5 bits (68), Expect = 0.47
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 3/39 (7%)
Frame = -1
Query: 299 HLLVVGMIWPPPLWMHQYG---VLHHRC*IEHHANSLQP 192
HL+V+G IW PP + +G L+H HH N+ P
Sbjct: 305 HLVVLGGIWVPPEYTSAHGGTPTLYHHQVHHHHTNTAGP 343
>At2g25730.1 68415.m03084 expressed protein
Length = 2464
Score = 29.9 bits (64), Expect = 1.4
Identities = 17/42 (40%), Positives = 20/42 (47%)
Frame = +3
Query: 63 FGALAPRVPAPTSWSIAPKEFSTSMKLRTLLWLDSSGPLRKE 188
F L P V A W + P + +T KL LLW S LR E
Sbjct: 422 FPRLRPLV-ASMGWDLLPGKTATRRKLMRLLWTSDSQALRLE 462
>At5g03415.1 68418.m00294 DPB-1 transcription factor, putative (DPB)
similar to Swiss-Prot:Q14186 transcription factor DP-1
[Homo sapiens]; contains Pfam profile PF02319:
Transcription factor E2F/dimerisation partner (TDP)
Length = 385
Score = 27.9 bits (59), Expect = 5.8
Identities = 15/64 (23%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
Frame = +2
Query: 113 SKGVQYLNEIKDSVVAGFQWAAKEGVMAEE---NLRGVRFNIYDVTLHTDASIEVVAKSF 283
SKG NE+ D +VA F +G ++ + + +R +YD L+ ++++++K
Sbjct: 117 SKGRTTYNEVADELVAEFALPNNDGTSPDQQQYDEKNIRRRVYD-ALNVLMAMDIISKDK 175
Query: 284 QQLE 295
++++
Sbjct: 176 KEIQ 179
>At5g07990.1 68418.m00930 flavonoid 3'-monooxygenase / flavonoid
3'-hydroxylase (F3'H) / cytochrome P450 75B1 (CYP75B1) /
transparent testa 7 protein (TT7) identical to SP|Q9SD85
Flavonoid 3'-monooxygenase (EC 1.14.13.21) (Flavonoid
3'-hydroxylase) (AtF3'H) (Cytochrome P450 75B1)
(TRANSPARENT TESTA 7 protein) {Arabidopsis thaliana};
similar to gi:10334806, gi:10334808
Length = 513
Score = 27.5 bits (58), Expect = 7.7
Identities = 13/42 (30%), Positives = 20/42 (47%)
Frame = +2
Query: 77 PEGTGPNILVDCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEE 202
P G G I S G++ + + ++V GF W GV E+
Sbjct: 437 PFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEK 478
>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase PG1 [Glycine max]
GI:5669846; contains PF00295: Glycosyl hydrolases family
28
Length = 444
Score = 27.5 bits (58), Expect = 7.7
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = +3
Query: 414 SWSRFRRVPGGRY-TYVHCEGL 476
S+ R R +PGGRY +++H +GL
Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,434,728
Number of Sequences: 28952
Number of extensions: 358833
Number of successful extensions: 948
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 912
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 945
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1265787216
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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