BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV40259 (658 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g16160.1 68415.m01852 hypothetical protein includes At2g06610... 29 2.1 At2g35110.1 68415.m04307 HEM protein-related weak similarity to ... 29 2.7 At3g59100.1 68416.m06589 glycosyl transferase family 48 protein ... 29 3.6 At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical ... 29 3.6 At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical ... 29 3.6 At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical ... 29 3.6 At2g28780.1 68415.m03499 expressed protein 29 3.6 At1g23090.1 68414.m02887 sulfate transporter, putative similar t... 29 3.6 At3g51895.1 68416.m05692 sulfate transporter (ST1) identical to ... 27 8.3 >At2g16160.1 68415.m01852 hypothetical protein includes At2g06610, At5g28266, At3g42620, At4g07696, At2g06690, At2g16160, At2g05480, At2g12140, At1g45080, At2g16330 Length = 217 Score = 29.5 bits (63), Expect = 2.1 Identities = 20/68 (29%), Positives = 26/68 (38%) Frame = +1 Query: 70 QFLTEDYANNGIELNNRFGDDASEKIPLKNLSKLPEFKIATQLPKDAEFSLFLPKHQEMA 249 QFL E IE+ N D K+P K + K F + Q + EF F + Sbjct: 114 QFLPEYVIGALIEVGNLTKDYRGRKLPFKIMDKQKFFNVKHQNDQAFEFQRFFQRLTGPQ 173 Query: 250 NDFLAFSW 273 N SW Sbjct: 174 NIIALVSW 181 >At2g35110.1 68415.m04307 HEM protein-related weak similarity to Membrane-associated protein Hem (Dhem-2) (Swiss-Prot:P55162) [Drosophila melanogaster]; weak similarity to Nck-associated protein 1 (NAP 1) (p125Nap1) (Membrane-associated protein HEM-2) (Swiss-Prot:P55161) [Rattus norvegicus] Length = 1339 Score = 29.1 bits (62), Expect = 2.7 Identities = 15/45 (33%), Positives = 22/45 (48%) Frame = +3 Query: 312 CAFARVNLNPQLFNYCYSVALMHRRDTRKVRVKNFAEVFPSKFLD 446 CAF RVNL + ++ DTRK+R + F + +F D Sbjct: 202 CAFVRVNLFAEKVLEAVGPSIFLSADTRKLRNEGFLSPYHPRFPD 246 >At3g59100.1 68416.m06589 glycosyl transferase family 48 protein contains Pfam profile: PF02364 1,3-beta-glucan synthase Length = 1934 Score = 28.7 bits (61), Expect = 3.6 Identities = 10/24 (41%), Positives = 15/24 (62%), Gaps = 2/24 (8%) Frame = +2 Query: 590 INLH--HYHWHLVYPFTANDLSIV 655 +NLH HY WH +P N++ +V Sbjct: 715 MNLHIGHYQWHEFFPHATNNIGVV 738 >At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical to protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana] Length = 453 Score = 28.7 bits (61), Expect = 3.6 Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 1/50 (2%) Frame = +1 Query: 166 KLPEFKIATQLPKDAEFSLFLPKHQEM-ANDFLAFSWMYQRTNYKICYRH 312 K+P++K ++ KD + LPK + DF + ++ +Q NY + RH Sbjct: 241 KIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRH 290 >At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical to protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana] Length = 467 Score = 28.7 bits (61), Expect = 3.6 Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 1/50 (2%) Frame = +1 Query: 166 KLPEFKIATQLPKDAEFSLFLPKHQEM-ANDFLAFSWMYQRTNYKICYRH 312 K+P++K ++ KD + LPK + DF + ++ +Q NY + RH Sbjct: 255 KIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRH 304 >At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical to protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana] Length = 467 Score = 28.7 bits (61), Expect = 3.6 Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 1/50 (2%) Frame = +1 Query: 166 KLPEFKIATQLPKDAEFSLFLPKHQEM-ANDFLAFSWMYQRTNYKICYRH 312 K+P++K ++ KD + LPK + DF + ++ +Q NY + RH Sbjct: 255 KIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRH 304 >At2g28780.1 68415.m03499 expressed protein Length = 796 Score = 28.7 bits (61), Expect = 3.6 Identities = 10/31 (32%), Positives = 19/31 (61%) Frame = +1 Query: 391 PEKSELRILQKYFPLNSWIPKYSLKLVKPQL 483 PE++++++L+ F SWI + K + P L Sbjct: 362 PEENKVKVLENKFKTRSWISDWDSKKIMPAL 392 >At1g23090.1 68414.m02887 sulfate transporter, putative similar to sulfate transporter [Arabidopsis thaliana] GI:2285885; contains Pfam profiles PF00916: Sulfate transporter family, PF01740: STAS domain Length = 631 Score = 28.7 bits (61), Expect = 3.6 Identities = 12/33 (36%), Positives = 20/33 (60%), Gaps = 1/33 (3%) Frame = +1 Query: 391 PEKSEL-RILQKYFPLNSWIPKYSLKLVKPQLL 486 P +++L R Q FP+ W P+YS L+K ++ Sbjct: 39 PNRTKLIRAAQYIFPILQWCPEYSFSLLKSDVV 71 >At3g51895.1 68416.m05692 sulfate transporter (ST1) identical to sulfate transporter [Arabidopsis thaliana] GI:2285885 Length = 658 Score = 27.5 bits (58), Expect = 8.3 Identities = 11/23 (47%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +1 Query: 421 KYF-PLNSWIPKYSLKLVKPQLL 486 KYF P+ W P+Y+LK K L+ Sbjct: 65 KYFLPIFEWAPRYNLKFFKSDLI 87 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,805,760 Number of Sequences: 28952 Number of extensions: 314474 Number of successful extensions: 878 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 867 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 878 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1373722560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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