Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40247
(598 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q2F6B1 Cluster: E2F transcription factor 4-like protein... 162 5e-39
UniRef50_UPI0001554BD5 Cluster: PREDICTED: hypothetical protein;... 50 4e-05
UniRef50_Q16254 Cluster: Transcription factor E2F4; n=21; Chorda... 48 2e-04
UniRef50_Q5DE83 Cluster: Putative uncharacterized protein; n=1; ... 47 4e-04
UniRef50_A7SL09 Cluster: Predicted protein; n=1; Nematostella ve... 47 4e-04
UniRef50_Q15329 Cluster: Transcription factor E2F5; n=23; Mammal... 45 0.001
UniRef50_UPI0000519C4F Cluster: PREDICTED: similar to Transcript... 44 0.003
UniRef50_O77051 Cluster: CG1071-PA; n=2; Sophophora|Rep: CG1071-... 42 0.008
UniRef50_UPI00015B53C3 Cluster: PREDICTED: similar to transcript... 40 0.059
UniRef50_Q4H3N6 Cluster: Transcription factor protein; n=2; Cion... 37 0.31
UniRef50_UPI000036135E Cluster: Transcription factor E2F1 (E2F-1... 37 0.41
UniRef50_UPI0000E48175 Cluster: PREDICTED: similar to E2F transc... 36 0.96
UniRef50_Q4T1B5 Cluster: Chromosome undetermined SCAF10684, whol... 35 1.7
UniRef50_Q9FRZ9 Cluster: E2F homolog; n=7; Embryophyta|Rep: E2F ... 35 1.7
UniRef50_Q4KMF0 Cluster: Zgc:111879 protein; n=12; Deuterostomia... 34 2.9
UniRef50_UPI00005A2AD0 Cluster: PREDICTED: similar to E2F transc... 33 3.9
UniRef50_Q4Z6G4 Cluster: Putative uncharacterized protein; n=1; ... 33 5.1
UniRef50_Q9FRZ8 Cluster: E2F homolog; n=17; Magnoliophyta|Rep: E... 32 8.9
>UniRef50_Q2F6B1 Cluster: E2F transcription factor 4-like protein;
n=1; Bombyx mori|Rep: E2F transcription factor 4-like
protein - Bombyx mori (Silk moth)
Length = 260
Score = 162 bits (394), Expect = 5e-39
Identities = 77/77 (100%), Positives = 77/77 (100%)
Frame = +1
Query: 1 IKQSTKNVLDEPDNQDMCFVMDTEIQQCFQNDLVLVLEAPLGANLSIGTQPNKDRESNFL 180
IKQSTKNVLDEPDNQDMCFVMDTEIQQCFQNDLVLVLEAPLGANLSIGTQPNKDRESNFL
Sbjct: 112 IKQSTKNVLDEPDNQDMCFVMDTEIQQCFQNDLVLVLEAPLGANLSIGTQPNKDRESNFL 171
Query: 181 LHIKSSESMGVVLLNDK 231
LHIKSSESMGVVLLNDK
Sbjct: 172 LHIKSSESMGVVLLNDK 188
Score = 137 bits (332), Expect = 2e-31
Identities = 62/62 (100%), Positives = 62/62 (100%)
Frame = +3
Query: 255 EDEETNSNNEMEDNNIKCFKNSEEASLINDCLLRLSPPASKYDFLFSLSNTEGLCELFDI 434
EDEETNSNNEMEDNNIKCFKNSEEASLINDCLLRLSPPASKYDFLFSLSNTEGLCELFDI
Sbjct: 197 EDEETNSNNEMEDNNIKCFKNSEEASLINDCLLRLSPPASKYDFLFSLSNTEGLCELFDI 256
Query: 435 PC 440
PC
Sbjct: 257 PC 258
>UniRef50_UPI0001554BD5 Cluster: PREDICTED: hypothetical protein;
n=1; Ornithorhynchus anatinus|Rep: PREDICTED:
hypothetical protein - Ornithorhynchus anatinus
Length = 384
Score = 50.0 bits (114), Expect = 4e-05
Identities = 22/77 (28%), Positives = 42/77 (54%)
Frame = +1
Query: 1 IKQSTKNVLDEPDNQDMCFVMDTEIQQCFQNDLVLVLEAPLGANLSIGTQPNKDRESNFL 180
++QS +NV ++ N + +V +I +CF D +L + AP G +L + + + +
Sbjct: 83 VQQSIRNVTEDVQNDHLAYVTHEDICRCFSGDTLLAIRAPSGTSLEVPFPEGPNGQKKYQ 142
Query: 181 LHIKSSESMGVVLLNDK 231
+H+KS+ VLL +K
Sbjct: 143 IHLKSTSGPIEVLLVNK 159
>UniRef50_Q16254 Cluster: Transcription factor E2F4; n=21;
Chordata|Rep: Transcription factor E2F4 - Homo sapiens
(Human)
Length = 413
Score = 47.6 bits (108), Expect = 2e-04
Identities = 22/76 (28%), Positives = 43/76 (56%), Gaps = 1/76 (1%)
Frame = +1
Query: 1 IKQSTKNVLDEPDNQDMCFVMDTEIQQCFQNDLVLVLEAPLGANLSIGTQPNKDRESNFL 180
++QS +NV ++ N + +V +I +CF D +L + AP G +L + + + +
Sbjct: 121 VQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAPSGTSLEVPIPEGLNGQKKYQ 180
Query: 181 LHIKS-SESMGVVLLN 225
+H+KS S + V+L+N
Sbjct: 181 IHLKSVSGPIEVLLVN 196
Score = 46.0 bits (104), Expect = 7e-04
Identities = 16/30 (53%), Positives = 25/30 (83%)
Frame = +3
Query: 348 LLRLSPPASKYDFLFSLSNTEGLCELFDIP 437
LLRLSPP +D++++L +EG+C+LFD+P
Sbjct: 380 LLRLSPPPGDHDYIYNLDESEGVCDLFDVP 409
>UniRef50_Q5DE83 Cluster: Putative uncharacterized protein; n=1;
Schistosoma japonicum|Rep: Putative uncharacterized
protein - Schistosoma japonicum (Blood fluke)
Length = 288
Score = 46.8 bits (106), Expect = 4e-04
Identities = 22/42 (52%), Positives = 31/42 (73%)
Frame = +3
Query: 312 KNSEEASLINDCLLRLSPPASKYDFLFSLSNTEGLCELFDIP 437
K +EE + ++ LL LSPP S+ DF ++L TEG+C+LFDIP
Sbjct: 241 KLNEENAFVSP-LLPLSPPPSECDFNYNLDETEGVCDLFDIP 281
Score = 32.7 bits (71), Expect = 6.7
Identities = 16/52 (30%), Positives = 26/52 (50%)
Frame = +1
Query: 1 IKQSTKNVLDEPDNQDMCFVMDTEIQQCFQNDLVLVLEAPLGANLSIGTQPN 156
+ QS +NV ++ DN +V ++ FQ+ +LV+ AP G L N
Sbjct: 128 VLQSLRNVQEDLDNLQHAYVTHQDLINIFQDRTMLVIRAPPGTRLEAPVPEN 179
>UniRef50_A7SL09 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 335
Score = 46.8 bits (106), Expect = 4e-04
Identities = 18/30 (60%), Positives = 25/30 (83%)
Frame = +3
Query: 348 LLRLSPPASKYDFLFSLSNTEGLCELFDIP 437
LLRLSPP + +D+ F+L + EG+C+LFDIP
Sbjct: 292 LLRLSPPPADHDYYFNLDDNEGVCDLFDIP 321
Score = 46.0 bits (104), Expect = 7e-04
Identities = 22/78 (28%), Positives = 43/78 (55%), Gaps = 1/78 (1%)
Frame = +1
Query: 1 IKQSTKNVLDEPDNQDMCFVMDTEIQQCFQNDLVLVLEAPLGANLSIG-TQPNKDRESNF 177
++QS KN+ ++P+N+ + FV ++ + F+ D +L ++AP G L + + +
Sbjct: 116 VQQSLKNISEDPENEKLAFVTYDDVCKSFKGDTLLAIQAPSGTQLEVPIPEQVPGMPKKY 175
Query: 178 LLHIKSSESMGVVLLNDK 231
+H+KS VLL +K
Sbjct: 176 QIHLKSQNGPIHVLLVNK 193
>UniRef50_Q15329 Cluster: Transcription factor E2F5; n=23;
Mammalia|Rep: Transcription factor E2F5 - Homo sapiens
(Human)
Length = 346
Score = 45.2 bits (102), Expect = 0.001
Identities = 23/77 (29%), Positives = 44/77 (57%), Gaps = 2/77 (2%)
Frame = +1
Query: 1 IKQSTKNVLDEPDNQDMCFVMDTEIQQCFQNDLVLVLEAPLGANLSIG-TQPNKDRESNF 177
++QS KNV+D+ N +V +I CF D +L ++AP G L + + ++ + +
Sbjct: 154 LQQSIKNVMDDSINNRFSYVTHEDICNCFNGDTLLAIQAPSGTQLEVPIPEMGQNGQKKY 213
Query: 178 LLHIKS-SESMGVVLLN 225
+++KS S + V+L+N
Sbjct: 214 QINLKSHSGPIHVLLIN 230
Score = 34.7 bits (76), Expect = 1.7
Identities = 17/30 (56%), Positives = 23/30 (76%), Gaps = 1/30 (3%)
Frame = +3
Query: 348 LLRLSP-PASKYDFLFSLSNTEGLCELFDI 434
LLRLSP PA Y+F +L + EG+C+LFD+
Sbjct: 314 LLRLSPTPADDYNF--NLDDNEGVCDLFDV 341
>UniRef50_UPI0000519C4F Cluster: PREDICTED: similar to Transcription
factor E2F5 (E2F-5); n=1; Apis mellifera|Rep: PREDICTED:
similar to Transcription factor E2F5 (E2F-5) - Apis
mellifera
Length = 300
Score = 44.0 bits (99), Expect = 0.003
Identities = 18/29 (62%), Positives = 24/29 (82%)
Frame = +3
Query: 348 LLRLSPPASKYDFLFSLSNTEGLCELFDI 434
L+RLSPP S+ D+ F+LS EG+C+LFDI
Sbjct: 269 LVRLSPPPSEKDYHFNLSENEGICDLFDI 297
Score = 33.9 bits (74), Expect = 2.9
Identities = 20/75 (26%), Positives = 40/75 (53%), Gaps = 9/75 (12%)
Frame = +1
Query: 1 IKQSTKNVLDEPDNQDMCFVMDTEIQQCFQNDLVLVLEAPLGANLSI---------GTQP 153
I+QS KN+ ++ N+ ++ ++++ F +D VL ++AP LS+ T
Sbjct: 112 IQQSIKNIENDLINRKHAYITYEDVKENFTDDFVLGIQAPPDTELSVPTYNKYVTTQTSE 171
Query: 154 NKDRESNFLLHIKSS 198
D+E N+ + +KS+
Sbjct: 172 EDDKEINYEMFLKST 186
>UniRef50_O77051 Cluster: CG1071-PA; n=2; Sophophora|Rep: CG1071-PA
- Drosophila melanogaster (Fruit fly)
Length = 370
Score = 42.3 bits (95), Expect = 0.008
Identities = 19/40 (47%), Positives = 28/40 (70%)
Frame = +3
Query: 321 EEASLINDCLLRLSPPASKYDFLFSLSNTEGLCELFDIPC 440
++ +L N LL+L+PP DF F+L + EG+CELFD+ C
Sbjct: 332 DDPNLENPPLLQLNPPQE--DFNFALKSDEGICELFDVQC 369
>UniRef50_UPI00015B53C3 Cluster: PREDICTED: similar to
transcriptional activator; n=1; Nasonia vitripennis|Rep:
PREDICTED: similar to transcriptional activator -
Nasonia vitripennis
Length = 447
Score = 39.5 bits (88), Expect = 0.059
Identities = 19/41 (46%), Positives = 27/41 (65%), Gaps = 2/41 (4%)
Frame = +3
Query: 318 SEEASLIN--DCLLRLSPPASKYDFLFSLSNTEGLCELFDI 434
S + S+++ D LL L PP S+ D+ FSL EGL +LFD+
Sbjct: 405 SSDMSILDFGDALLSLEPPLSESDYSFSLGTEEGLSDLFDL 445
>UniRef50_Q4H3N6 Cluster: Transcription factor protein; n=2; Ciona
intestinalis|Rep: Transcription factor protein - Ciona
intestinalis (Transparent sea squirt)
Length = 441
Score = 37.1 bits (82), Expect = 0.31
Identities = 16/38 (42%), Positives = 25/38 (65%)
Frame = +3
Query: 321 EEASLINDCLLRLSPPASKYDFLFSLSNTEGLCELFDI 434
+ A + D + LSP + D+LFSL ++EG+ ELFD+
Sbjct: 393 DSAFIDEDSFIALSPAMNPDDYLFSLDDSEGISELFDM 430
>UniRef50_UPI000036135E Cluster: Transcription factor E2F1 (E2F-1)
(Retinoblastoma-binding protein 3) (RBBP-3) (PRB-binding
protein E2F-1) (PBR3) (Retinoblastoma-associated protein
1) (RBAP-1).; n=2; Clupeocephala|Rep: Transcription
factor E2F1 (E2F-1) (Retinoblastoma-binding protein 3)
(RBBP-3) (PRB-binding protein E2F-1) (PBR3)
(Retinoblastoma-associated protein 1) (RBAP-1). -
Takifugu rubripes
Length = 428
Score = 36.7 bits (81), Expect = 0.41
Identities = 20/41 (48%), Positives = 25/41 (60%)
Frame = +3
Query: 309 FKNSEEASLINDCLLRLSPPASKYDFLFSLSNTEGLCELFD 431
F N E SL D + LSPP S +D+ F L + EG+ ELFD
Sbjct: 382 FLNGEGFSLPLDGFINLSPPHS-HDYHFGLEDHEGISELFD 421
>UniRef50_UPI0000E48175 Cluster: PREDICTED: similar to E2F
transcription factor 3; n=1; Strongylocentrotus
purpuratus|Rep: PREDICTED: similar to E2F transcription
factor 3 - Strongylocentrotus purpuratus
Length = 404
Score = 35.5 bits (78), Expect = 0.96
Identities = 16/38 (42%), Positives = 28/38 (73%)
Frame = +3
Query: 318 SEEASLINDCLLRLSPPASKYDFLFSLSNTEGLCELFD 431
+E+ + ++D + LSPPA D+LF+L++ EG+ +LFD
Sbjct: 363 TEDQNGLDDDFVALSPPAVD-DYLFALNDNEGISDLFD 399
>UniRef50_Q4T1B5 Cluster: Chromosome undetermined SCAF10684, whole
genome shotgun sequence; n=1; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF10684,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 316
Score = 34.7 bits (76), Expect = 1.7
Identities = 19/41 (46%), Positives = 24/41 (58%)
Frame = +3
Query: 309 FKNSEEASLINDCLLRLSPPASKYDFLFSLSNTEGLCELFD 431
F N E L D + LSPP S +D+ F L + EG+ ELFD
Sbjct: 270 FLNGEGFPLPLDGFINLSPPHS-HDYHFGLEDHEGISELFD 309
>UniRef50_Q9FRZ9 Cluster: E2F homolog; n=7; Embryophyta|Rep: E2F
homolog - Oryza sativa (Rice)
Length = 436
Score = 34.7 bits (76), Expect = 1.7
Identities = 15/62 (24%), Positives = 36/62 (58%), Gaps = 2/62 (3%)
Frame = +1
Query: 1 IKQSTKNVLDEPDNQDMCFVMDTEIQ--QCFQNDLVLVLEAPLGANLSIGTQPNKDRESN 174
+++ + + ++ +NQ +V + +I+ CFQN+ ++ ++AP G L + P+ D +
Sbjct: 212 MREKLRGLTEDENNQRWLYVTEDDIKGLPCFQNETLIAIKAPHGTTLEV---PDPDEAGD 268
Query: 175 FL 180
+L
Sbjct: 269 YL 270
>UniRef50_Q4KMF0 Cluster: Zgc:111879 protein; n=12;
Deuterostomia|Rep: Zgc:111879 protein - Danio rerio
(Zebrafish) (Brachydanio rerio)
Length = 363
Score = 33.9 bits (74), Expect = 2.9
Identities = 15/29 (51%), Positives = 21/29 (72%)
Frame = +3
Query: 348 LLRLSPPASKYDFLFSLSNTEGLCELFDI 434
LLRLSP D+ F+L + EG+C+LFD+
Sbjct: 331 LLRLSPNPG-VDYSFNLDDNEGVCDLFDV 358
>UniRef50_UPI00005A2AD0 Cluster: PREDICTED: similar to E2F
transcription factor 4; n=1; Canis lupus familiaris|Rep:
PREDICTED: similar to E2F transcription factor 4 - Canis
familiaris
Length = 358
Score = 33.5 bits (73), Expect = 3.9
Identities = 14/61 (22%), Positives = 29/61 (47%)
Frame = +1
Query: 13 TKNVLDEPDNQDMCFVMDTEIQQCFQNDLVLVLEAPLGANLSIGTQPNKDRESNFLLHIK 192
T ++ N + +V +I +CF D +L + AP +L + + + + +H+K
Sbjct: 113 TGQAQEDVQNSCLAYVTHEDICRCFAGDTLLAVWAPSATSLEVAIPEGLNGQKKYQIHLK 172
Query: 193 S 195
S
Sbjct: 173 S 173
>UniRef50_Q4Z6G4 Cluster: Putative uncharacterized protein; n=1;
Plasmodium berghei|Rep: Putative uncharacterized protein
- Plasmodium berghei
Length = 1526
Score = 33.1 bits (72), Expect = 5.1
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = -1
Query: 529 NIYLIYMFYMYNIKCLQKVVIAFSIKIYNQQGISKSSHNP 410
NI +I F+ + QK I+ SI IY+++ KS HNP
Sbjct: 736 NILIIRKFFRIFLNYAQKKFISLSIYIYSKKSKKKSLHNP 775
>UniRef50_Q9FRZ8 Cluster: E2F homolog; n=17; Magnoliophyta|Rep: E2F
homolog - Oryza sativa (Rice)
Length = 393
Score = 32.3 bits (70), Expect = 8.9
Identities = 14/61 (22%), Positives = 34/61 (55%), Gaps = 2/61 (3%)
Frame = +1
Query: 1 IKQSTKNVLDEPDNQDMCFVMDTEIQQ--CFQNDLVLVLEAPLGANLSIGTQPNKDRESN 174
++ + + ++ +NQ +V + +I+ CFQN ++ ++AP G L + P+ D ++
Sbjct: 179 MRDKLRGLTEDENNQKWLYVTEDDIKSLPCFQNQTLIAIKAPHGTTLEV---PDPDEVND 235
Query: 175 F 177
+
Sbjct: 236 Y 236
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 510,999,510
Number of Sequences: 1657284
Number of extensions: 8980531
Number of successful extensions: 28035
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 25255
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27900
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 41902926763
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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