BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV40247 (598 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_1618| Best HMM Match : No HMM Matches (HMM E-Value=.) 47 1e-05 SB_17246| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.2 SB_28275| Best HMM Match : REJ (HMM E-Value=0.0012) 28 5.0 SB_31428| Best HMM Match : Tetraspannin (HMM E-Value=0) 28 5.0 SB_24762| Best HMM Match : Astacin (HMM E-Value=0) 28 6.6 SB_4188| Best HMM Match : 7tm_1 (HMM E-Value=9e-06) 28 6.6 >SB_1618| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1371 Score = 46.8 bits (106), Expect = 1e-05 Identities = 18/30 (60%), Positives = 25/30 (83%) Frame = +3 Query: 348 LLRLSPPASKYDFLFSLSNTEGLCELFDIP 437 LLRLSPP + +D+ F+L + EG+C+LFDIP Sbjct: 276 LLRLSPPPADHDYYFNLDDNEGVCDLFDIP 305 Score = 46.0 bits (104), Expect = 2e-05 Identities = 22/78 (28%), Positives = 43/78 (55%), Gaps = 1/78 (1%) Frame = +1 Query: 1 IKQSTKNVLDEPDNQDMCFVMDTEIQQCFQNDLVLVLEAPLGANLSIG-TQPNKDRESNF 177 ++QS KN+ ++P+N+ + FV ++ + F+ D +L ++AP G L + + + Sbjct: 117 VQQSLKNISEDPENEKLAFVTYDDVCKSFKGDTLLAIQAPSGTQLEVPIPEQVPGMPKKY 176 Query: 178 LLHIKSSESMGVVLLNDK 231 +H+KS VLL +K Sbjct: 177 QIHLKSQNGPIHVLLVNK 194 >SB_17246| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 602 Score = 29.5 bits (63), Expect = 2.2 Identities = 12/35 (34%), Positives = 24/35 (68%) Frame = +3 Query: 261 EETNSNNEMEDNNIKCFKNSEEASLINDCLLRLSP 365 E+T++++ ++NNIK KN +++ ++ L RL P Sbjct: 270 EDTHTSHPSKNNNIKKLKNGAKSTAKDESLKRLKP 304 >SB_28275| Best HMM Match : REJ (HMM E-Value=0.0012) Length = 1551 Score = 28.3 bits (60), Expect = 5.0 Identities = 11/35 (31%), Positives = 21/35 (60%) Frame = -1 Query: 499 YNIKCLQKVVIAFSIKIYNQQGISKSSHNPSVLLK 395 Y +KC + + I +YN++ K+SH+ ++LK Sbjct: 311 YRVKCEFLLCHQYPIGVYNEEMTIKTSHSSDLILK 345 >SB_31428| Best HMM Match : Tetraspannin (HMM E-Value=0) Length = 280 Score = 28.3 bits (60), Expect = 5.0 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = +1 Query: 1 IKQSTKNVLDEPDNQDMCFVMDTEIQQC 84 +KQ K LD+P NQD+ M T ++ C Sbjct: 142 LKQDLKKYLDDPLNQDLIDFMQTLLKCC 169 >SB_24762| Best HMM Match : Astacin (HMM E-Value=0) Length = 733 Score = 27.9 bits (59), Expect = 6.6 Identities = 12/41 (29%), Positives = 21/41 (51%) Frame = -1 Query: 517 IYMFYMYNIKCLQKVVIAFSIKIYNQQGISKSSHNPSVLLK 395 I ++ + L+ F I +N QG SSH+PS++ + Sbjct: 263 ILTYFQQMLPILETDESLFCISAWNDQGYEHSSHDPSMMYR 303 >SB_4188| Best HMM Match : 7tm_1 (HMM E-Value=9e-06) Length = 522 Score = 27.9 bits (59), Expect = 6.6 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = -1 Query: 535 LCNIYLIYMFYMYNIKCLQKVVIAF 461 +CN L+YM Y ++CL+K V F Sbjct: 2 ICNGGLLYMLYKDPLRCLRKPVTVF 26 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,099,685 Number of Sequences: 59808 Number of extensions: 286745 Number of successful extensions: 735 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 624 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 725 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1439498375 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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