Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV40225
         (574 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   147   1e-36
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   142   2e-35
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    52   5e-08
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    35   0.007
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    31   0.16 
SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S...    28   1.1  
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl...    27   1.5  
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch...    26   3.4  
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po...    25   6.0  
SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po...    25   7.9  
SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces...    25   7.9  

>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  147 bits (356), Expect = 1e-36
 Identities = 62/85 (72%), Positives = 75/85 (88%)
 Frame = -2

Query: 510 PRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGG 331
           PR G+YMA C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP  VPG 
Sbjct: 311 PRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGS 370

Query: 330 DLAKVQRAVCMLSNTTAIAEAWARL 256
            +AKV RAVCMLSNTT+IAEAW+RL
Sbjct: 371 GIAKVNRAVCMLSNTTSIAEAWSRL 395



 Score =  100 bits (240), Expect = 1e-22
 Identities = 44/47 (93%), Positives = 46/47 (97%)
 Frame = -1

Query: 253 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGMDS 113
           HKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALE+DYEEVG DS
Sbjct: 397 HKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDS 443



 Score = 45.2 bits (102), Expect = 7e-06
 Identities = 20/31 (64%), Positives = 22/31 (70%)
 Frame = -3

Query: 569 SVAEITNACFEPANQMVKCDPVMASTWLAVC 477
           SV EITN CFEP NQMVKCDP     ++A C
Sbjct: 291 SVQEITNQCFEPYNQMVKCDP-RTGRYMATC 320


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  142 bits (345), Expect = 2e-35
 Identities = 61/85 (71%), Positives = 73/85 (85%)
 Frame = -2

Query: 510 PRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGG 331
           PR G+YMA C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI  +PP  + G 
Sbjct: 307 PRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGS 366

Query: 330 DLAKVQRAVCMLSNTTAIAEAWARL 256
           ++AKV RAVCMLSNTT+IAEAW+RL
Sbjct: 367 EIAKVDRAVCMLSNTTSIAEAWSRL 391



 Score =  100 bits (240), Expect = 1e-22
 Identities = 44/47 (93%), Positives = 46/47 (97%)
 Frame = -1

Query: 253 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGMDS 113
           HKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALE+DYEEVG DS
Sbjct: 393 HKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDS 439



 Score = 46.8 bits (106), Expect = 2e-06
 Identities = 21/31 (67%), Positives = 23/31 (74%)
 Frame = -3

Query: 569 SVAEITNACFEPANQMVKCDPVMASTWLAVC 477
           SV EITN CFEP NQMVKCDP  A  ++A C
Sbjct: 287 SVQEITNQCFEPYNQMVKCDP-RAGRYMATC 316


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 52.4 bits (120), Expect = 5e-08
 Identities = 22/48 (45%), Positives = 33/48 (68%), Gaps = 3/48 (6%)
 Frame = -1

Query: 250 KFDLMYAKRAFVHWYVGEGMEEGEFSEAR---EDLAALEKDYEEVGMD 116
           +F  M+ ++AF+HWY GEGM+E EF+EA     DL +  + Y+E G+D
Sbjct: 384 QFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAGID 431



 Score = 51.6 bits (118), Expect = 8e-08
 Identities = 28/85 (32%), Positives = 45/85 (52%)
 Frame = -2

Query: 510 PRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGG 331
           PRHG+Y+    L+RG V  K+V+  I +++TK +  FV+W P      +   PP      
Sbjct: 305 PRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK----- 359

Query: 330 DLAKVQRAVCMLSNTTAIAEAWARL 256
           DL   + +   + N+T+I E + RL
Sbjct: 360 DL---KMSATFIGNSTSIQEIFRRL 381


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 35.1 bits (77), Expect = 0.007
 Identities = 18/73 (24%), Positives = 38/73 (52%)
 Frame = -2

Query: 477 LYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCM 298
           + +G+  P DV+ ++  I+ +R   F+ W P   +V ++ + P +     ++ +     M
Sbjct: 323 IIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGL-----M 377

Query: 297 LSNTTAIAEAWAR 259
           L+N T+IA  + R
Sbjct: 378 LANHTSIASLFKR 390


>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 30.7 bits (66), Expect = 0.16
 Identities = 16/45 (35%), Positives = 21/45 (46%)
 Frame = -3

Query: 470 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 336
           VV   P +  RP++P  P  LS     V PV+  V +   PP  P
Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596



 Score = 25.4 bits (53), Expect = 6.0
 Identities = 20/68 (29%), Positives = 26/68 (38%)
 Frame = -3

Query: 536 PANQMVKCDPVMASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTT 357
           PA  +V   P +     A     V S P+    P +P  PSV    +  V P + SV   
Sbjct: 518 PAAPVVPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQP 577

Query: 356 SHPPWCPE 333
              P  PE
Sbjct: 578 PVAPVAPE 585



 Score = 25.0 bits (52), Expect = 7.9
 Identities = 14/45 (31%), Positives = 18/45 (40%)
 Frame = -3

Query: 470 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 336
           VV   P +  RP++P  P   S       PV   V +   PP  P
Sbjct: 642 VVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686


>SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein
           Sad1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 514

 Score = 27.9 bits (59), Expect = 1.1
 Identities = 18/66 (27%), Positives = 30/66 (45%), Gaps = 9/66 (13%)
 Frame = +1

Query: 388 TPVDELDSTLGFDGSDGR--------VHILGYDVTTVQHTASHVLAMTGSHFTIWLAG-S 540
           TP + +     F GS+G+        V++    +  VQH  +H L+     F +W+ G S
Sbjct: 365 TPNNAVAMCWSFQGSEGQLGISLSRPVYVTNVTIEHVQHKIAHDLSSAPKDFELWVQGMS 424

Query: 541 KHAFVI 558
              FV+
Sbjct: 425 SKMFVL 430


>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
           subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 709

 Score = 27.5 bits (58), Expect = 1.5
 Identities = 20/61 (32%), Positives = 27/61 (44%), Gaps = 2/61 (3%)
 Frame = -2

Query: 252 TSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVREPKST 79
           TS TS T S       S++     S+P P   W P+        S+ TP+   V EP+ T
Sbjct: 153 TSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVDEPRFT 212

Query: 78  K 76
           K
Sbjct: 213 K 213


>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 628

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
 Frame = -3

Query: 434 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 348
           +LP KPS+   +++S   V+P S   STTS+P
Sbjct: 5   TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36


>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 665

 Score = 25.4 bits (53), Expect = 6.0
 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
 Frame = -3

Query: 437 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 342
           P  PS+P+++SN ST  G+Q V   V   +   W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564


>SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 793

 Score = 25.0 bits (52), Expect = 7.9
 Identities = 22/68 (32%), Positives = 28/68 (41%)
 Frame = -2

Query: 513 RPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPG 334
           R RH  + A   LY  +   KDVN  +       TI   +W PTG ++   Y     V  
Sbjct: 140 RWRHSSF-AQYYLYNTET--KDVNM-LGQDNEHWTISLAEWSPTGHQLSFVYNNDLYVRK 195

Query: 333 GDLAKVQR 310
            D   VQR
Sbjct: 196 ND-GNVQR 202


>SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 476

 Score = 25.0 bits (52), Expect = 7.9
 Identities = 15/37 (40%), Positives = 16/37 (43%)
 Frame = -3

Query: 455 PRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPP 345
           PR      LP K  VL    T +  VSR   TTS  P
Sbjct: 293 PRPLLNSKLPRKTLVLDLDETLIHSVSRGSRTTSGQP 329


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,436,641
Number of Sequences: 5004
Number of extensions: 50837
Number of successful extensions: 196
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 244081442
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)