BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV40221 (598 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC824.06 |tim14||TIM23 translocase complex subunit Tim14|Schiz... 28 1.2 SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc... 27 2.7 SPBC1734.12c |alg12||dolichyl pyrophosphate Man7GlcNAc2 alpha-1,... 26 3.6 SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac... 26 3.6 SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe... 26 4.8 SPAC23H4.10c |thi4||thiamine-phosphate dipyrophosphorylase/hydro... 26 4.8 SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces po... 25 6.3 SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual 25 6.3 SPBC1734.16c |pst3||SIN3 family co-repressor|Schizosaccharomyces... 25 8.4 >SPAC824.06 |tim14||TIM23 translocase complex subunit Tim14|Schizosaccharomyces pombe|chr 1|||Manual Length = 140 Score = 27.9 bits (59), Expect = 1.2 Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 2/50 (4%) Frame = +2 Query: 287 KTEGFDLLSKECLALAREKLRDGGRRLG-AEFP-RGGEPSAARRMSAEMS 430 + E +LS L R+K+++ RRL A P RGG P A +++ S Sbjct: 49 RAEAIQILSLNNRTLTRQKIKEAHRRLMLANHPDRGGSPYVASKVNEAKS 98 >SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1063 Score = 26.6 bits (56), Expect = 2.7 Identities = 13/38 (34%), Positives = 24/38 (63%) Frame = -2 Query: 447 LFSVCSDISADIRLAADGSPPLGNSAPKRLPPSRSFSL 334 L ++C+ I+A LA +G+PP+ + +PP+R S+ Sbjct: 207 LNNLCNQITA--LLARNGNPPIPMQKLQSMPPARLISI 242 >SPBC1734.12c |alg12||dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase Alg12 |Schizosaccharomyces pombe|chr 2|||Manual Length = 546 Score = 26.2 bits (55), Expect = 3.6 Identities = 19/42 (45%), Positives = 23/42 (54%), Gaps = 1/42 (2%) Frame = -3 Query: 449 YCFQYAQTFPPTSALQQTVLRL*ETQ-HPSVSLHRGVFPLPT 327 Y FQYA +P AL RL E + HP VS+H V+P T Sbjct: 358 YVFQYA--YPGGLALT----RLYEIENHPQVSVHMDVYPCMT 393 >SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 406 Score = 26.2 bits (55), Expect = 3.6 Identities = 13/39 (33%), Positives = 20/39 (51%) Frame = -2 Query: 432 SDISADIRLAADGSPPLGNSAPKRLPPSRSFSLANARHS 316 S ISA + G S P++ PP+ +FS AR++ Sbjct: 190 SQISASLAWLKTGGASAAPSNPRQPPPTSNFSNRKARYA 228 >SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 871 Score = 25.8 bits (54), Expect = 4.8 Identities = 12/46 (26%), Positives = 20/46 (43%) Frame = +3 Query: 456 PPLGLAG*PGDVLSHEDVLLYECEKHPREHEWDEAAIGDRIQRDLS 593 PP A + + V + CE + H W A + ++RD+S Sbjct: 768 PPAPGAAKAAVMFTPPKVHYFTCENNEELHRWSSAFLKATVERDMS 813 >SPAC23H4.10c |thi4||thiamine-phosphate dipyrophosphorylase/hydroxyethylthiazole kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 518 Score = 25.8 bits (54), Expect = 4.8 Identities = 11/23 (47%), Positives = 16/23 (69%) Frame = -2 Query: 441 SVCSDISADIRLAADGSPPLGNS 373 +V + SA++ LAA GSP +G S Sbjct: 262 AVAKNFSANVTLAAYGSPTMGES 284 >SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 365 Score = 25.4 bits (53), Expect = 6.3 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = +3 Query: 228 AAHWPLPAIPGRIPI*SPKLKRKVSIYYQR 317 AA+ PLP +P R SP +R+ Y+R Sbjct: 172 AANEPLPEVPSRFRSRSPPARRRYRDDYRR 201 >SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 1496 Score = 25.4 bits (53), Expect = 6.3 Identities = 8/15 (53%), Positives = 11/15 (73%) Frame = +1 Query: 460 HLDLPGNPVTCYHMK 504 H DL NP +CYH++ Sbjct: 1258 HYDLLHNPASCYHIR 1272 >SPBC1734.16c |pst3||SIN3 family co-repressor|Schizosaccharomyces pombe|chr 2|||Manual Length = 1154 Score = 25.0 bits (52), Expect = 8.4 Identities = 11/38 (28%), Positives = 21/38 (55%) Frame = -2 Query: 405 AADGSPPLGNSAPKRLPPSRSFSLANARHSFDNKSKPS 292 + + S P +P RLPP +FSL + + +++P+ Sbjct: 325 STEPSTPNVQKSPNRLPPVGNFSLPPSAPVREKRNRPA 362 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,589,713 Number of Sequences: 5004 Number of extensions: 53370 Number of successful extensions: 148 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 148 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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