Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV40209
         (800 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   129   4e-31
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   129   4e-31
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   129   4e-31
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    52   1e-07
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    32   0.11 
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo...    28   1.8  
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    27   4.1  
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E...    27   4.1  
SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma...    26   5.4  
SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    26   7.2  
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos...    25   9.5  

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  129 bits (312), Expect = 4e-31
 Identities = 58/78 (74%), Positives = 68/78 (87%)
 Frame = +1

Query: 271 HTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 450
           HTAHIACKFAE+ EK+DRR+GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421

Query: 451 FAVRDMRQTVAVGVIKAV 504
           FAVRDMRQTVAVGVIKAV
Sbjct: 422 FAVRDMRQTVAVGVIKAV 439



 Score =  122 bits (294), Expect = 6e-29
 Identities = 58/83 (69%), Positives = 67/83 (80%)
 Frame = +3

Query: 36  TEVKSVGDATHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVI 215
           TEVKSV +  HE+L   +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI
Sbjct: 285 TEVKSV-EMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVI 343

Query: 216 VLNHPGQISNGYTQLLDCPHCPH 284
           +LNHPGQIS GY+ +LDC H  H
Sbjct: 344 ILNHPGQISAGYSPVLDC-HTAH 365


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  129 bits (312), Expect = 4e-31
 Identities = 58/78 (74%), Positives = 68/78 (87%)
 Frame = +1

Query: 271 HTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 450
           HTAHIACKFAE+ EK+DRR+GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421

Query: 451 FAVRDMRQTVAVGVIKAV 504
           FAVRDMRQTVAVGVIKAV
Sbjct: 422 FAVRDMRQTVAVGVIKAV 439



 Score =  122 bits (294), Expect = 6e-29
 Identities = 58/83 (69%), Positives = 67/83 (80%)
 Frame = +3

Query: 36  TEVKSVGDATHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVI 215
           TEVKSV +  HE+L   +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI
Sbjct: 285 TEVKSV-EMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVI 343

Query: 216 VLNHPGQISNGYTQLLDCPHCPH 284
           +LNHPGQIS GY+ +LDC H  H
Sbjct: 344 ILNHPGQISAGYSPVLDC-HTAH 365


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  129 bits (312), Expect = 4e-31
 Identities = 58/78 (74%), Positives = 68/78 (87%)
 Frame = +1

Query: 271 HTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 450
           HTAHIACKFAE+ EK+DRR+GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421

Query: 451 FAVRDMRQTVAVGVIKAV 504
           FAVRDMRQTVAVGVIKAV
Sbjct: 422 FAVRDMRQTVAVGVIKAV 439



 Score =  122 bits (294), Expect = 6e-29
 Identities = 58/83 (69%), Positives = 67/83 (80%)
 Frame = +3

Query: 36  TEVKSVGDATHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVI 215
           TEVKSV +  HE+L   +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI
Sbjct: 285 TEVKSV-EMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVI 343

Query: 216 VLNHPGQISNGYTQLLDCPHCPH 284
           +LNHPGQIS GY+ +LDC H  H
Sbjct: 344 ILNHPGQISAGYSPVLDC-HTAH 365


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 52.0 bits (119), Expect = 1e-07
 Identities = 28/80 (35%), Positives = 45/80 (56%), Gaps = 1/80 (1%)
 Frame = +1

Query: 271 HTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 450
           HTA     FA++  K+D+ T + ++  P     G   I  L    P+C+E F+++  +GR
Sbjct: 584 HTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGR 642

Query: 451 FAVRDMRQTVAVG-VIKAVN 507
           F +RD   TVAVG V+K ++
Sbjct: 643 FTLRDQGTTVAVGKVVKILD 662



 Score = 39.1 bits (87), Expect = 7e-04
 Identities = 21/72 (29%), Positives = 36/72 (50%)
 Frame = +3

Query: 39  EVKSVGDATHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIV 218
           EV ++ D   E +  ++ GD V   V+     +++ GYV   +KN P      F AQ+ +
Sbjct: 509 EVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAI 566

Query: 219 LNHPGQISNGYT 254
           L  P  ++ GY+
Sbjct: 567 LELPSILTTGYS 578


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 31.9 bits (69), Expect = 0.11
 Identities = 15/31 (48%), Positives = 20/31 (64%)
 Frame = +1

Query: 406 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 498
           PLC+   +E P LGRF +R    TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589


>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1336

 Score = 27.9 bits (59), Expect = 1.8
 Identities = 16/55 (29%), Positives = 29/55 (52%)
 Frame = -1

Query: 200 KICSTLRWVVFGVTSNITTTQFLDGHVLYVETYIVSRYSFLESFVGGISHRFDFS 36
           K  S ++ V+F    +     F D      ++++  R+SFL++F GG+S  F +S
Sbjct: 295 KSISAIQTVIFHSMQDTEYRYFADACSTSSKSFL--RFSFLDAFQGGVSQFFLYS 347


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
           Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 26.6 bits (56), Expect = 4.1
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = -1

Query: 140 QFLDGHVLYVETYIVSRYSFLESFV 66
           +F DGH+L  ETY+      LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551


>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
           EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 439

 Score = 26.6 bits (56), Expect = 4.1
 Identities = 10/27 (37%), Positives = 18/27 (66%)
 Frame = +3

Query: 75  LQEAVPGDNVGFNVKNVSVKELRRGYV 155
           L  AV GDN G  ++++  ++L+RG +
Sbjct: 308 LDAAVAGDNCGLLLRSIKREQLKRGMI 334


>SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 918

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 13/32 (40%), Positives = 19/32 (59%)
 Frame = +1

Query: 178 HLRVLQILQLKSLCLTILVKSQTVTHSYWIAH 273
           HLR +  L LK   L+IL   + ++  +WIAH
Sbjct: 637 HLRRVLGLSLKLYHLSILYFFELISSIFWIAH 668


>SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 398

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 13/40 (32%), Positives = 20/40 (50%)
 Frame = +3

Query: 165 SKNNPPKGAADFTAQVIVLNHPGQISNGYTQLLDCPHCPH 284
           ++ NPP   +     VIV     Q+S   +Q   CP+CP+
Sbjct: 349 TEENPPMMMS--CGHVIVKESLRQLSRNGSQRFKCPYCPN 386


>SPAC631.01c |acp2||F-actin capping protein beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 268

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = -1

Query: 383 MAASPDLMDFGLTSVDLPVRRSTFS 309
           ++ +PDL D  L+SVD P++ +T S
Sbjct: 27  LSVAPDLADVLLSSVDQPLKVNTCS 51


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,390,067
Number of Sequences: 5004
Number of extensions: 70335
Number of successful extensions: 203
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 203
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)