Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40205
(811 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1) 101 6e-22
At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1) (... 53 3e-07
At1g77440.1 68414.m09018 20S proteasome beta subunit C (PBC2) id... 52 6e-07
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 33 0.22
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 33 0.22
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 31 0.90
At3g22630.1 68416.m02857 20S proteasome beta subunit D (PBD1) (P... 31 0.90
At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2) (... 30 2.1
At4g26920.1 68417.m03874 hypothetical protein 29 2.8
At1g63990.1 68414.m07248 DNA topoisomerase VIA, putative (SPO11-... 29 3.7
At5g22760.1 68418.m02658 PHD finger family protein contains Pfam... 28 6.4
At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2) ... 28 8.4
>At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1)
Length = 223
Score = 101 bits (242), Expect = 6e-22
Identities = 42/86 (48%), Positives = 61/86 (70%)
Frame = +3
Query: 252 IGATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLA 431
+ ++G D L ++L++R +Y+H+HNK M+ PA+AQ+LS LY+KRFFPYY NVL
Sbjct: 58 LSSSGFQADVKALQKVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLG 117
Query: 432 GLDSDGKGCVYSYDPIGHCARHNFRA 509
GLD +GKGCV++YD +G R + A
Sbjct: 118 GLDEEGKGCVFTYDAVGSYERVGYGA 143
Score = 70.5 bits (165), Expect = 1e-12
Identities = 33/56 (58%), Positives = 42/56 (75%), Gaps = 1/56 (1%)
Frame = +1
Query: 91 KQH-RFEPYADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVL 255
KQH + PY +NGG+ VAIAG DY VI ADTR+STG+SI +RD K+ KL++ VL
Sbjct: 3 KQHANWSPYDNNGGTCVAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKLADRAVL 58
>At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1)
(PRCT) almost identical to GB:AAC32069 from [Arabidopsis
thaliana], EST gb|T76747 comes from this gene; identical
to cDNA proteasome subunit prct GI:2511567
Length = 204
Score = 52.8 bits (121), Expect = 3e-07
Identities = 28/77 (36%), Positives = 40/77 (51%)
Frame = +3
Query: 252 IGATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLA 431
IG +G D TL + L R ++Y+ + M A ++S +LY KRF PY V+A
Sbjct: 51 IGLSGLATDVQTLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPYLCQPVIA 110
Query: 432 GLDSDGKGCVYSYDPIG 482
GL D K + + D IG
Sbjct: 111 GLGDDDKPFICTMDSIG 127
>At1g77440.1 68414.m09018 20S proteasome beta subunit C (PBC2)
identical to residues 14-204 of 20S proteasome beta
subunit PBC2 GB:AAC32069 [Arabidopsis thaliana]
Length = 204
Score = 51.6 bits (118), Expect = 6e-07
Identities = 27/77 (35%), Positives = 40/77 (51%)
Frame = +3
Query: 252 IGATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLA 431
IG +G D TL + L R ++Y+ + M A ++S +LY KRF P+ V+A
Sbjct: 51 IGLSGLATDVQTLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPFLCQPVIA 110
Query: 432 GLDSDGKGCVYSYDPIG 482
GL D K + + D IG
Sbjct: 111 GLGDDNKPFICTMDSIG 127
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 33.1 bits (72), Expect = 0.22
Identities = 23/86 (26%), Positives = 40/86 (46%), Gaps = 1/86 (1%)
Frame = +3
Query: 210 QRPKKAVQII*EYSIGA-TGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTML 386
Q KK ++I Y +G G D R L + +++E + + ++ +++L+ ML
Sbjct: 86 QSVKKIIEIN-PYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLANML 144
Query: 387 YYKRFFPYYVSNVLAGLDSDGKGCVY 464
Y R V ++AG D G G Y
Sbjct: 145 YSYRGMGLSVGTMIAGWDETGPGLYY 170
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 33.1 bits (72), Expect = 0.22
Identities = 23/86 (26%), Positives = 40/86 (46%), Gaps = 1/86 (1%)
Frame = +3
Query: 210 QRPKKAVQII*EYSIGA-TGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTML 386
Q KK ++I Y +G G D R L + +++E + + ++ +++L+ ML
Sbjct: 86 QSVKKIIEIN-PYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLANML 144
Query: 387 YYKRFFPYYVSNVLAGLDSDGKGCVY 464
Y R V ++AG D G G Y
Sbjct: 145 YSYRGMGLSVGTMIAGWDETGPGLYY 170
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 31.1 bits (67), Expect = 0.90
Identities = 14/46 (30%), Positives = 27/46 (58%)
Frame = +1
Query: 124 GGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLER 261
G +I+ + + V+GAD+R STG + R K+ +L+++ + R
Sbjct: 12 GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCR 57
>At3g22630.1 68416.m02857 20S proteasome beta subunit D (PBD1)
(PRGB) identical to GB:CAA74026 from [Arabidopsis
thaliana] ( FEBS Lett. (1997) 416 (3), 281-285);
identical to cDNA proteasome subunit prgb GI:2511589
Length = 204
Score = 31.1 bits (67), Expect = 0.90
Identities = 21/77 (27%), Positives = 35/77 (45%), Gaps = 1/77 (1%)
Frame = +3
Query: 252 IGATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYK-RFFPYYVSNVL 428
+ A+G D + T +Q + +Y+ + +TT A A L R PY V+ ++
Sbjct: 44 VAASGEPGDRVQFTEYVQKNVSLYKFRNGIPLTTAAAANFTRGELATALRKNPYSVNILM 103
Query: 429 AGLDSDGKGCVYSYDPI 479
AG D + +Y D I
Sbjct: 104 AGYDDESGASLYYIDYI 120
>At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2)
(PRCGA) identical to SP|O24633 Proteasome subunit beta
type 2-2 (EC 3.4.25.1) (20S proteasome alpha subunit D2)
{Arabidopsis thaliana}, cDNA proteasome subunit prcga
GI:2511571
Length = 199
Score = 29.9 bits (64), Expect = 2.1
Identities = 20/77 (25%), Positives = 35/77 (45%), Gaps = 1/77 (1%)
Frame = +3
Query: 252 IGATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYK-RFFPYYVSNVL 428
+ A+G D + T +Q + +Y+ + ++T A A L R PY V+ ++
Sbjct: 44 VAASGEPGDRVQFTEYVQKNVSLYQFRNGIPLSTAAAANFTRGELATALRKNPYSVNILM 103
Query: 429 AGLDSDGKGCVYSYDPI 479
AG D + +Y D I
Sbjct: 104 AGYDKEAGASLYYIDYI 120
>At4g26920.1 68417.m03874 hypothetical protein
Length = 461
Score = 29.5 bits (63), Expect = 2.8
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +3
Query: 366 QMLSTMLYYKRFFPYYVSNV 425
+ML+ YY RFFP+Y NV
Sbjct: 93 EMLTLNEYYSRFFPWYAKNV 112
>At1g63990.1 68414.m07248 DNA topoisomerase VIA, putative (SPO11-2)
similar to topoisomerase 6 subunit A (spo11)
[Arabidopsis thaliana] GI:12331186; contains Pfam
profile PF04406: Type IIB DNA topoisomerase; identical
to cDNA putative topoisomerase VIA (SPO11 gene 2)
GI:7270976
Length = 383
Score = 29.1 bits (62), Expect = 3.7
Identities = 12/49 (24%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = -2
Query: 201 GKSSAETSVCSDNGIIIAGD-GYNTTSVVRVGLETMLFRTRHSIFRKVL 58
GK + + S C +G I GD +++R ++ +H+IF +++
Sbjct: 178 GKEAVDCSACGSSGFAITGDLNLLDNTIMRTDARYIIIVEKHAIFHRLV 226
>At5g22760.1 68418.m02658 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 1566
Score = 28.3 bits (60), Expect = 6.4
Identities = 12/31 (38%), Positives = 21/31 (67%)
Frame = -1
Query: 346 IDLLCSCSYICILACRRRVKVNVSHQQPVAP 254
+++L +C C LA R R ++N++H+QP P
Sbjct: 1122 LEILVTCKR-CYLA-RARSQININHRQPTTP 1150
>At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2)
identical to GB:AF043519
Length = 246
Score = 27.9 bits (59), Expect = 8.4
Identities = 18/84 (21%), Positives = 38/84 (45%), Gaps = 4/84 (4%)
Frame = +3
Query: 258 ATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLS--TMLYYKRFF--PYYVSNV 425
ATG D+ +L + + + ++ M +A+ ++ + +Y + + P V +
Sbjct: 80 ATGMTADSRSLVQQARNEAAEFRFQYGYEMPADILAKWIADKSQVYTQHAYMRPLGVVAM 139
Query: 426 LAGLDSDGKGCVYSYDPIGHCARH 497
+ G+D + +Y DP GH H
Sbjct: 140 VLGIDEERGPLLYKCDPAGHFYGH 163
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,377,821
Number of Sequences: 28952
Number of extensions: 400791
Number of successful extensions: 999
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 967
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 999
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1843581600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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