BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdV40188 (624 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 154 1e-38 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 154 1e-38 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 154 1e-38 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 60 2e-10 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 48 1e-06 SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 2.2 SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 2.9 SPAC20G8.01 |cdc17||ATP-dependent DNA ligase Cdc17|Schizosacchar... 27 2.9 SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 5.1 SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 25 6.7 SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 6.7 SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 25 8.9 SPBC405.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 8.9 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 154 bits (373), Expect = 1e-38 Identities = 71/85 (83%), Positives = 77/85 (90%) Frame = +2 Query: 2 LRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPG 181 LRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PG Sbjct: 244 LRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPG 303 Query: 182 DNVGFNVKNVSVKELRRGYVAGDSK 256 DNVGFNVKNVSVK++RRG V GDSK Sbjct: 304 DNVGFNVKNVSVKDIRRGNVCGDSK 328 Score = 142 bits (344), Expect = 4e-35 Identities = 62/95 (65%), Positives = 76/95 (80%) Frame = +1 Query: 241 CW*LQNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 420 C +N+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383 Query: 421 STEVNPKSIKSGDSAIVNLVPSKPLCVESSRNSHP 525 E +PK +KSGD+ I +VPSKP+CVE+ + P Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418 Score = 42.3 bits (95), Expect = 5e-05 Identities = 23/43 (53%), Positives = 26/43 (60%), Gaps = 1/43 (2%) Frame = +3 Query: 495 MC-RVLQEFPPLGRFAVRDMRQTVAVESSRLSTSRRVGVAKVT 620 MC ++ PLGRFAVRDMRQTVAV + G AKVT Sbjct: 408 MCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAKVT 450 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 154 bits (373), Expect = 1e-38 Identities = 71/85 (83%), Positives = 77/85 (90%) Frame = +2 Query: 2 LRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPG 181 LRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PG Sbjct: 244 LRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPG 303 Query: 182 DNVGFNVKNVSVKELRRGYVAGDSK 256 DNVGFNVKNVSVK++RRG V GDSK Sbjct: 304 DNVGFNVKNVSVKDIRRGNVCGDSK 328 Score = 142 bits (344), Expect = 4e-35 Identities = 62/95 (65%), Positives = 76/95 (80%) Frame = +1 Query: 241 CW*LQNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 420 C +N+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383 Query: 421 STEVNPKSIKSGDSAIVNLVPSKPLCVESSRNSHP 525 E +PK +KSGD+ I +VPSKP+CVE+ + P Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418 Score = 42.3 bits (95), Expect = 5e-05 Identities = 23/43 (53%), Positives = 26/43 (60%), Gaps = 1/43 (2%) Frame = +3 Query: 495 MC-RVLQEFPPLGRFAVRDMRQTVAVESSRLSTSRRVGVAKVT 620 MC ++ PLGRFAVRDMRQTVAV + G AKVT Sbjct: 408 MCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAKVT 450 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 154 bits (373), Expect = 1e-38 Identities = 71/85 (83%), Positives = 77/85 (90%) Frame = +2 Query: 2 LRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPG 181 LRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PG Sbjct: 244 LRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPG 303 Query: 182 DNVGFNVKNVSVKELRRGYVAGDSK 256 DNVGFNVKNVSVK++RRG V GDSK Sbjct: 304 DNVGFNVKNVSVKDIRRGNVCGDSK 328 Score = 142 bits (344), Expect = 4e-35 Identities = 62/95 (65%), Positives = 76/95 (80%) Frame = +1 Query: 241 CW*LQNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 420 C +N+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383 Query: 421 STEVNPKSIKSGDSAIVNLVPSKPLCVESSRNSHP 525 E +PK +KSGD+ I +VPSKP+CVE+ + P Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418 Score = 42.3 bits (95), Expect = 5e-05 Identities = 23/43 (53%), Positives = 26/43 (60%), Gaps = 1/43 (2%) Frame = +3 Query: 495 MC-RVLQEFPPLGRFAVRDMRQTVAVESSRLSTSRRVGVAKVT 620 MC ++ PLGRFAVRDMRQTVAV + G AKVT Sbjct: 408 MCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAKVT 450 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 60.5 bits (140), Expect = 2e-10 Identities = 31/86 (36%), Positives = 49/86 (56%), Gaps = 2/86 (2%) Frame = +2 Query: 8 LPLQDVYKIGGIGTVPVGRVETGVLKPGTIV--VFAPANITTEVKSVEMHHEALQEAVPG 181 + ++DV+ I G GTV GRVE G LK G + V +++ T V +EM + L AV G Sbjct: 255 MAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAG 314 Query: 182 DNVGFNVKNVSVKELRRGYVAGDSKT 259 DN G ++++ ++L+RG + T Sbjct: 315 DNCGLLLRSIKREQLKRGMIVAQPGT 340 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 48.0 bits (109), Expect = 1e-06 Identities = 25/82 (30%), Positives = 40/82 (48%) Frame = +1 Query: 259 NPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNP 438 NP F AQ+ +L P ++ GY+ V+ HTA FA++ K+D +T + ++ P Sbjct: 552 NPVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLD-KTNRKSKKPP 610 Query: 439 KSIKSGDSAIVNLVPSKPLCVE 504 G I L P+C+E Sbjct: 611 MFATKGMKIIAELETQTPVCME 632 Score = 42.3 bits (95), Expect = 5e-05 Identities = 30/108 (27%), Positives = 54/108 (50%), Gaps = 7/108 (6%) Frame = +2 Query: 8 LPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSV-EMHHEALQEAVPGD 184 +P+ YK +GT+ G++E G +K + V+ P N T EV ++ + E + ++ GD Sbjct: 471 MPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGD 528 Query: 185 NVGFNVKNVSVKELRRGYVAGDSK------TTHLRVLQILQLKSLCLT 310 V V+ +++ GYV +K T + + IL+L S+ T Sbjct: 529 QVRLRVRG-DDSDVQTGYVLTSTKNPVHATTRFIAQIAILELPSILTT 575 >SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosaccharomyces pombe|chr 2|||Manual Length = 948 Score = 27.1 bits (57), Expect = 2.2 Identities = 14/39 (35%), Positives = 22/39 (56%) Frame = -1 Query: 132 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 16 +SVV+ + +T V + + VST TGTV +P +C Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124 >SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a Pol2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 2199 Score = 26.6 bits (56), Expect = 2.9 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = -3 Query: 226 QFLDGHVLYVETYIVSRYSFLESFV 152 +F DGH+L ETY+ LES V Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551 >SPAC20G8.01 |cdc17||ATP-dependent DNA ligase Cdc17|Schizosaccharomyces pombe|chr 1|||Manual Length = 768 Score = 26.6 bits (56), Expect = 2.9 Identities = 14/33 (42%), Positives = 18/33 (54%), Gaps = 1/33 (3%) Frame = -2 Query: 545 TDSKTTEGWE-FLEDSTHRGLEGTKLTMAESPD 450 +D K+ E E FLE+S EG + M E PD Sbjct: 547 SDQKSIESIEEFLEESVKDSCEGLMVKMLEGPD 579 >SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1233 Score = 25.8 bits (54), Expect = 5.1 Identities = 10/39 (25%), Positives = 22/39 (56%) Frame = -3 Query: 250 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 134 +T ++ + L H+ +++ SR +FL ++H+H F Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050 >SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1372 Score = 25.4 bits (53), Expect = 6.7 Identities = 12/46 (26%), Positives = 24/46 (52%) Frame = +1 Query: 394 EKVDRRTGKSTEVNPKSIKSGDSAIVNLVPSKPLCVESSRNSHPSV 531 EKV+ + + + +S+K D I N S + +E+ +N+ P + Sbjct: 36 EKVEAKALSRSRLKNQSVKKTDLRITNDYSSLFVSIENKKNTIPDI 81 >SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity factor complex subunit Rna14|Schizosaccharomyces pombe|chr 1|||Manual Length = 733 Score = 25.4 bits (53), Expect = 6.7 Identities = 10/18 (55%), Positives = 12/18 (66%) Frame = -1 Query: 81 NTPVSTLPTGTVPIPPIL 28 N P S LPT VP+P I+ Sbjct: 666 NPPTSALPTVPVPLPSII 683 >SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 25.0 bits (52), Expect = 8.9 Identities = 13/38 (34%), Positives = 16/38 (42%) Frame = -1 Query: 168 SWRASWCISTDLTSVVMLAGAKTTMVPGFNTPVSTLPT 55 SW SW +ST L +V + G T ST T Sbjct: 97 SWNPSWMVSTILVGLVSFMTSDEITTGGIVTSESTRRT 134 >SPBC405.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 143 Score = 25.0 bits (52), Expect = 8.9 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = +1 Query: 391 KEKVDRRTGKSTEVNPKSIKSGDSAIVNL 477 +EKV +RT S +N + ++ D ++NL Sbjct: 39 EEKVTQRTASSDSLNTIASENNDENVINL 67 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,811,191 Number of Sequences: 5004 Number of extensions: 61030 Number of successful extensions: 188 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 175 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 187 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 275671126 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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