Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40174
(740 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso... 148 4e-36
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 142 3e-34
At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso... 136 1e-32
At2g44065.2 68415.m05480 ribosomal protein L2 family protein sim... 59 3e-09
At2g44065.1 68415.m05479 ribosomal protein L2 family protein sim... 59 3e-09
At4g14250.1 68417.m02198 UBX domain-containing protein low simil... 34 0.11
At3g27670.1 68416.m03455 expressed protein 30 1.9
At4g32600.1 68417.m04642 zinc finger (C3HC4-type RING finger) fa... 29 2.4
At2g45270.1 68415.m05635 glycoprotease M22 family protein simila... 29 3.2
At1g75030.1 68414.m08715 pathogenesis-related thaumatin family p... 29 3.2
At5g65290.1 68418.m08212 LMBR1 integral membrane family protein ... 28 5.7
At4g33410.1 68417.m04748 signal peptide peptidase family protein... 28 5.7
At5g02140.1 68418.m00135 thaumatin-like protein, putative simila... 28 7.5
At1g71180.1 68414.m08213 6-phosphogluconate dehydrogenase NAD-bi... 28 7.5
At1g19320.1 68414.m02402 pathogenesis-related thaumatin family p... 28 7.5
At4g38660.1 68417.m05473 thaumatin, putative similar to thaumati... 27 9.9
At4g05360.1 68417.m00818 zinc knuckle (CCHC-type) family protein... 27 9.9
At3g21940.1 68416.m02767 receptor protein kinase-related contain... 27 9.9
>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
Length = 260
Score = 148 bits (358), Expect = 4e-36
Identities = 91/203 (44%), Positives = 118/203 (58%), Gaps = 6/203 (2%)
Frame = +2
Query: 41 MGRVIRAQRKGA-GSVFVSHTKKRXGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLA 217
MGRVIRAQRKGA GSVF SHT R G K RSLDY ER+GY+KG+V +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAAGSVFKSHTHHRKGPAKFRSLDYGERNGYLKGLVTEIIHDPGRGAPLA 60
Query: 218 VVHFRDPYKFKTRRSSSLLPKALHRPICL-LWKESNS*SWKCDACWSYA*G-YHC---VQ 382
V FR P+++ ++ + + ++ L K++N S G C +
Sbjct: 61 RVAFRHPFRYMKQKELFVAAEGMYTGQYLYCGKKANLMVGNVLPLGSIPEGAVICNVELH 120
Query: 383 S*RENGX*RSSGTCLWKLRHCDWTQS*C*AYKSKATVWSQEGLPSSNRGMVGIVAGGGRI 562
R+SG + H + + + K S++ LPS+ R M+G VAGGGR
Sbjct: 121 VGDRGALARASGDYAIVIAHNPESNT----TRVKLPSGSKKILPSACRAMIGQVAGGGRT 176
Query: 563 DKPILKAGRAYHKYKVKRNCWAI 631
+KP LKAG AYHKYK KRNCW +
Sbjct: 177 EKPFLKAGNAYHKYKAKRNCWPV 199
Score = 144 bits (349), Expect = 5e-35
Identities = 69/143 (48%), Positives = 92/143 (64%)
Frame = +3
Query: 249 RQEGALHCSRRLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGXRGRLARAS 428
+Q+ + +YTGQ++YCGKKA L VGNV+P+G++PEG ++CN+E +G RG LARAS
Sbjct: 72 KQKELFVAAEGMYTGQYLYCGKKANLMVGNVLPLGSIPEGAVICNVELHVGDRGALARAS 131
Query: 429 GNFATVIGHNPDAKRTRVKLPSGAKKVCHQATEAWSVLLLEVDVLTNLF*KLEGHTTSTR 608
G++A VI HNP++ TRVKLPSG+KK+ A A + F K +
Sbjct: 132 GDYAIVIAHNPESNTTRVKLPSGSKKILPSACRAMIGQVAGGGRTEKPFLKAGNAYHKYK 191
Query: 609 SNVTAGPYVRGVAMNPVEHPHGG 677
+ P VRGVAMNPVEHPHGG
Sbjct: 192 AKRNCWPVVRGVAMNPVEHPHGG 214
Score = 33.1 bits (72), Expect = 0.20
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = +1
Query: 676 GGNHQHIGKASTVKR 720
GGNHQHIG ASTV+R
Sbjct: 214 GGNHQHIGHASTVRR 228
>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)
Length = 258
Score = 142 bits (343), Expect = 3e-34
Identities = 70/143 (48%), Positives = 90/143 (62%)
Frame = +3
Query: 249 RQEGALHCSRRLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGXRGRLARAS 428
+Q+ + +YTGQF+YCGKKATL VGNV+P+ ++PEG +VCN+E +G RG LARAS
Sbjct: 71 KQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVVCNVEHHVGDRGVLARAS 130
Query: 429 GNFATVIGHNPDAKRTRVKLPSGAKKVCHQATEAWSVLLLEVDVLTNLF*KLEGHTTSTR 608
G++A VI HNPD+ TR+KLPSG+KK+ A + K R
Sbjct: 131 GDYAIVIAHNPDSDTTRIKLPSGSKKIVPSGCRAMIGQVAGGGRTEKPMLKAGNAYHKYR 190
Query: 609 SNVTAGPYVRGVAMNPVEHPHGG 677
+ P VRGVAMNPVEHPHGG
Sbjct: 191 VKRNSWPKVRGVAMNPVEHPHGG 213
Score = 113 bits (271), Expect = 2e-25
Identities = 52/73 (71%), Positives = 60/73 (82%)
Frame = +2
Query: 41 MGRVIRAQRKGAGSVFVSHTKKRXGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 220
MGRVIRAQRKGAGSVF SHT R G K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLAR 60
Query: 221 VHFRDPYKFKTRR 259
V FR P++FK ++
Sbjct: 61 VTFRHPFRFKKQK 73
Score = 33.1 bits (72), Expect = 0.20
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = +1
Query: 676 GGNHQHIGKASTVKR 720
GGNHQHIG ASTV+R
Sbjct: 213 GGNHQHIGHASTVRR 227
>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
protein L8, cytosolic, tomato, PIR1:R5TOL8
Length = 258
Score = 136 bits (330), Expect = 1e-32
Identities = 67/143 (46%), Positives = 87/143 (60%)
Frame = +3
Query: 249 RQEGALHCSRRLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGXRGRLARAS 428
+Q+ + +YTGQF+YCGKKATL VGNV+P+ ++PEG ++CN+E +G RG ARAS
Sbjct: 71 KQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVICNVEHHVGDRGVFARAS 130
Query: 429 GNFATVIGHNPDAKRTRVKLPSGAKKVCHQATEAWSVLLLEVDVLTNLF*KLEGHTTSTR 608
G++A VI HNPD +R+KLPSG+KK+ A + K R
Sbjct: 131 GDYAIVIAHNPDNDTSRIKLPSGSKKIVPSGCRAMIGQVAGGGRTEKPMLKAGNAYHKYR 190
Query: 609 SNVTAGPYVRGVAMNPVEHPHGG 677
P VRGVAMNPVEHPHGG
Sbjct: 191 VKRNCWPKVRGVAMNPVEHPHGG 213
Score = 113 bits (271), Expect = 2e-25
Identities = 52/73 (71%), Positives = 60/73 (82%)
Frame = +2
Query: 41 MGRVIRAQRKGAGSVFVSHTKKRXGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 220
MGRVIRAQRKGAGSVF SHT R G K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLAR 60
Query: 221 VHFRDPYKFKTRR 259
V FR P++FK ++
Sbjct: 61 VAFRHPFRFKKQK 73
Score = 33.1 bits (72), Expect = 0.20
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = +1
Query: 676 GGNHQHIGKASTVKR 720
GGNHQHIG ASTV+R
Sbjct: 213 GGNHQHIGHASTVRR 227
>At2g44065.2 68415.m05480 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 59.3 bits (137), Expect = 3e-09
Identities = 41/116 (35%), Positives = 57/116 (49%)
Frame = +3
Query: 330 VGNVMPVGAMPEGTIVCNLEEKMGXRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKV 509
+G+ MP+G M GTI+ N+E G ++ RA+G A ++ P + +KLPSG K
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKW 117
Query: 510 CHQATEAWSVLLLEVDVLTNLF*KLEGHTTSTRSNVTAGPYVRGVAMNPVEHPHGG 677
+ A + T KL S + P VRGVAMNP +HPHGG
Sbjct: 118 INAKCRATIGTVSNPSHGTK---KLYKAGQSRWLGIR--PKVRGVAMNPCDHPHGG 168
>At2g44065.1 68415.m05479 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 59.3 bits (137), Expect = 3e-09
Identities = 41/116 (35%), Positives = 57/116 (49%)
Frame = +3
Query: 330 VGNVMPVGAMPEGTIVCNLEEKMGXRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKV 509
+G+ MP+G M GTI+ N+E G ++ RA+G A ++ P + +KLPSG K
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKW 117
Query: 510 CHQATEAWSVLLLEVDVLTNLF*KLEGHTTSTRSNVTAGPYVRGVAMNPVEHPHGG 677
+ A + T KL S + P VRGVAMNP +HPHGG
Sbjct: 118 INAKCRATIGTVSNPSHGTK---KLYKAGQSRWLGIR--PKVRGVAMNPCDHPHGG 168
>At4g14250.1 68417.m02198 UBX domain-containing protein low
similarity to 60S ribosomal protein L2 [Nicotiana
tabacum] GI:9230281; contains Pfam profile PF00789: UBX
domain
Length = 724
Score = 33.9 bits (74), Expect = 0.11
Identities = 15/16 (93%), Positives = 15/16 (93%), Gaps = 1/16 (6%)
Frame = +3
Query: 633 VRGVAM-NPVEHPHGG 677
VRGVAM NPVEHPHGG
Sbjct: 422 VRGVAMMNPVEHPHGG 437
>At3g27670.1 68416.m03455 expressed protein
Length = 1841
Score = 29.9 bits (64), Expect = 1.9
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = -1
Query: 344 HHISNFKSCFLSTINKLACVEPSGAMKSSFLS*TCMDRGSV 222
H + + CF I++L V SGA++ L +C+ G +
Sbjct: 1204 HEVDDIIGCFKDLISELLIVRKSGALRKRLLMASCIGAGDL 1244
>At4g32600.1 68417.m04642 zinc finger (C3HC4-type RING finger)
family protein low similarity to RING finger protein 38
[Homo sapiens] GI:21666412; contains Pfam profile
PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 453
Score = 29.5 bits (63), Expect = 2.4
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = +1
Query: 169 SC*GYNP*PW*RCTFGCCTLPRSIQVQDKKELFIAPEGSTQANL 300
SC GY P+ CT CC LP I + +E P G+T ++
Sbjct: 274 SCIGYAM-PFILCTTICCCLPCIISILGYREDLTQPRGATPESI 316
>At2g45270.1 68415.m05635 glycoprotease M22 family protein similar
to SP|P36175 O-sialoglycoprotein endopeptidase (EC
3.4.24.57) (Glycoprotease) {Pasteurella haemolytica};
contains Pfam profile PF00814: Glycoprotease family
Length = 480
Score = 29.1 bits (62), Expect = 3.2
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = -3
Query: 597 WYALPAFKIGLSIRPPPATIPTMPLLLDGRPSW 499
W L F++G PPPAT P + D RP W
Sbjct: 409 WTGLEHFRVGRYDPPPPATEPE-DYVYDLRPRW 440
>At1g75030.1 68414.m08715 pathogenesis-related thaumatin family
protein identical to thaumatin-like protein [Arabidopsis
thaliana] GI:2435406; contains Pfam profile: PF00314
Thaumatin family
Length = 246
Score = 29.1 bits (62), Expect = 3.2
Identities = 13/45 (28%), Positives = 22/45 (48%)
Frame = -2
Query: 682 YHPP*GCSTGFMATPRTYGPAVTFDLVLVVCPSSFQNRFVNTSTS 548
+ P C TG ATP+T P + CPS++ + + S++
Sbjct: 185 FSSPEFCCTGAHATPQTCSPTYYSSMFKNACPSAYSYAYDDASST 229
>At5g65290.1 68418.m08212 LMBR1 integral membrane family protein
contains Pfam PF04791: LMBR1-like conserved region
Length = 733
Score = 28.3 bits (60), Expect = 5.7
Identities = 8/21 (38%), Positives = 13/21 (61%)
Frame = +1
Query: 547 WRWTY*QTYFESWKGIPQVQG 609
W W+Y T+ +W +P +QG
Sbjct: 74 WSWSYWSTFLLTWAVVPLIQG 94
>At4g33410.1 68417.m04748 signal peptide peptidase family protein
contains Pfam domain PF04258: Membrane protein of
unknown function (DUF435)
Length = 372
Score = 28.3 bits (60), Expect = 5.7
Identities = 17/44 (38%), Positives = 22/44 (50%), Gaps = 2/44 (4%)
Frame = -3
Query: 603 YLWYALPAFKIGL--SIRPPPATIPTMPLLLDGRPSWLQTVALL 478
Y+WYALP + IGL ++ T P LL PS L V +
Sbjct: 301 YIWYALPGYAIGLVAALAAGVLTHSPQPALLYLVPSTLGPVIFM 344
>At5g02140.1 68418.m00135 thaumatin-like protein, putative similar
to SP|P50699 Thaumatin-like protein precursor
{Arabidopsis thaliana}; contains Pfam profile PF00314:
Thaumatin family
Length = 294
Score = 27.9 bits (59), Expect = 7.5
Identities = 11/38 (28%), Positives = 20/38 (52%)
Frame = -2
Query: 664 CSTGFMATPRTYGPAVTFDLVLVVCPSSFQNRFVNTST 551
C TG A P+ P + +L +CP ++ F ++S+
Sbjct: 189 CCTGEYANPQACKPTLFANLFKAICPKAYSYAFDDSSS 226
>At1g71180.1 68414.m08213 6-phosphogluconate dehydrogenase
NAD-binding domain-containing protein similar to
SP|P23523 2-hydroxy-3-oxopropionate reductase (EC
1.1.1.60) (Tartronate semialdehyde reductase)
{Escherichia coli}; contains Pfam profile PF03446: NAD
binding domain of 6-phosphogluconate dehydrogenase
Length = 318
Score = 27.9 bits (59), Expect = 7.5
Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 2/34 (5%)
Frame = +2
Query: 536 GIVAGGGRIDKPILKAG--RAYHKYKVKRNCWAI 631
G+ GG +D K G R H +RNCWA+
Sbjct: 117 GLTPGGVTVDMTSSKPGLAREIHAEARRRNCWAV 150
>At1g19320.1 68414.m02402 pathogenesis-related thaumatin family
protein similar to SP:P28493 Pathogenesis-related
protein 5 precursor (PR-5) from [Arabidopsis thaliana],
thaumatin-like protein [Arabidopsis thaliana]
GI:2435406; contains Pfam profile PF00314: Thaumatin
family
Length = 247
Score = 27.9 bits (59), Expect = 7.5
Identities = 13/52 (25%), Positives = 25/52 (48%)
Frame = -2
Query: 688 DGYHPP*GCSTGFMATPRTYGPAVTFDLVLVVCPSSFQNRFVNTSTSSNNTD 533
+ ++ P C TG +TP T P + CPS++ + + S++ T+
Sbjct: 187 EAFNKPEYCCTGAYSTPATCPPTNYSKIFKQACPSAYSYAYDDASSTFTCTN 238
>At4g38660.1 68417.m05473 thaumatin, putative similar to
thaumatin-like protein [Arabidopsis thaliana]
GI:2435406, thaumatin-like protein precursor [Pyrus
pyrifolia] GI:3241854; contains Pfam profile PF00314:
Thaumatin family
Length = 345
Score = 27.5 bits (58), Expect = 9.9
Identities = 14/45 (31%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
Frame = -2
Query: 682 YHPP*GCSTGFMATPRTYGPAVTFDLVLVVCPSSFQNRFVN-TST 551
+ P C +G ATP + P+V ++ CP S+ + + TST
Sbjct: 187 FRSPEYCCSGAYATPSSCRPSVYSEMFKAACPRSYSYAYDDATST 231
>At4g05360.1 68417.m00818 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 735
Score = 27.5 bits (58), Expect = 9.9
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = +2
Query: 608 VKRNCWAICTWCCHEPCRASSRWV 679
+KRNC+ C HE R + WV
Sbjct: 673 IKRNCFRFIRECSHEGLRRNKVWV 696
>At3g21940.1 68416.m02767 receptor protein kinase-related contains
Pfam profile: PF01657 Domain of unknown function
Length = 275
Score = 27.5 bits (58), Expect = 9.9
Identities = 15/53 (28%), Positives = 28/53 (52%), Gaps = 3/53 (5%)
Frame = -3
Query: 327 QELLSFHNKQIGLCRAFGSNEELLLVLNLYGSRKCT---TAKGAPLPGSWIIS 178
Q+L K++G+ + + E+ L + LYG +CT + K + +WII+
Sbjct: 192 QDLPEIGGKRLGMKMLYATGEKSLRTMKLYGMVQCTKDLSIKDCIVCLNWIIA 244
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,417,489
Number of Sequences: 28952
Number of extensions: 383711
Number of successful extensions: 1023
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 983
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1016
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1633819784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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