Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40173
(827 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g48330.2 68416.m05275 protein-L-isoaspartate O-methyltransfer... 93 2e-19
At3g48330.1 68416.m05274 protein-L-isoaspartate O-methyltransfer... 93 2e-19
At5g50240.1 68418.m06222 protein-L-isoaspartate O-methyltransfer... 75 6e-14
At1g73320.1 68414.m08485 expressed protein 32 0.40
At1g23360.1 68414.m02923 UbiE/COQ5 methyltransferase family prot... 32 0.40
At5g52490.1 68418.m06512 fibrillarin, putative similar to fibril... 32 0.53
At3g62000.1 68416.m06963 O-methyltransferase family 3 protein se... 32 0.53
At5g14600.1 68418.m01712 expressed protein 31 0.71
At5g64150.1 68418.m08055 methylase family protein contains TIGRf... 31 0.93
At1g17145.1 68414.m02090 expressed protein 31 0.93
At5g49020.2 68418.m06066 protein arginine N-methyltransferase fa... 30 2.2
At5g49020.1 68418.m06065 protein arginine N-methyltransferase fa... 30 2.2
At5g15540.1 68418.m01819 expressed protein low similarity to DNA... 29 2.8
At3g61990.1 68416.m06962 O-methyltransferase family 3 protein se... 29 2.8
At5g54400.1 68418.m06775 expressed protein 29 3.8
At4g26590.1 68417.m03833 oligopeptide transporter OPT family pro... 29 3.8
At3g15530.2 68416.m01969 expressed protein 29 3.8
At3g15530.1 68416.m01968 expressed protein 29 3.8
At4g26220.1 68417.m03775 caffeoyl-CoA 3-O-methyltransferase, put... 28 6.6
At5g59990.1 68418.m07523 expressed protein ; expression supporte... 28 8.7
At1g24735.1 68414.m03105 caffeoyl-CoA 3-O-methyltransferase, put... 28 8.7
>At3g48330.2 68416.m05275 protein-L-isoaspartate O-methyltransferase
/ PIMT (PCM) identical to SP|Q42539
Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)
(Protein- beta-aspartate methyltransferase) (PIMT)
(Protein L-isoaspartyl methyltransferase) (L-isoaspartyl
protein carboxyl methyltransferase) {Arabidopsis
thaliana}
Length = 230
Score = 93.5 bits (222), Expect = 2e-19
Identities = 44/78 (56%), Positives = 54/78 (69%)
Frame = +3
Query: 258 TISAPHMHAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVN 437
TISAPHMHA L+ L+ L PG + LDVGSG+GYLTAC A+M+G GR +G+EHI ELV
Sbjct: 63 TISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPELVA 122
Query: 438 LATKNIQNDNPSLLSSER 491
+ KNI+ S ER
Sbjct: 123 SSVKNIEASAASPFLKER 140
Score = 83.8 bits (198), Expect = 1e-16
Identities = 42/70 (60%), Positives = 50/70 (71%)
Frame = +2
Query: 509 DGRLGYPSEAPYSAIHVGAAAPTLPQALIDQLKPGGRLIVPVGPEGGEQHLTQVDKAQDG 688
DGR G+ APY AIHVGAAAP +P+ALIDQLKPGGRL++PVG Q L VDK DG
Sbjct: 148 DGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLKPGGRLVIPVG--NIFQDLQVVDKNSDG 205
Query: 689 TTTVKKLMSV 718
+ ++K SV
Sbjct: 206 SVSIKDETSV 215
Score = 46.4 bits (105), Expect = 2e-05
Identities = 25/55 (45%), Positives = 34/55 (61%), Gaps = 2/55 (3%)
Frame = +1
Query: 115 NVDLIRNLRTNGIIKSDTVANAMLAVDRKNYCP--SSPYQDSPQSIGFSQQSALP 273
N ++ NL+ +GI+ SD VA AM AVDR + SS Y DSP SIG++ + P
Sbjct: 13 NKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGYNVTISAP 67
>At3g48330.1 68416.m05274 protein-L-isoaspartate O-methyltransferase
/ PIMT (PCM) identical to SP|Q42539
Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)
(Protein- beta-aspartate methyltransferase) (PIMT)
(Protein L-isoaspartyl methyltransferase) (L-isoaspartyl
protein carboxyl methyltransferase) {Arabidopsis
thaliana}
Length = 230
Score = 93.5 bits (222), Expect = 2e-19
Identities = 44/78 (56%), Positives = 54/78 (69%)
Frame = +3
Query: 258 TISAPHMHAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVN 437
TISAPHMHA L+ L+ L PG + LDVGSG+GYLTAC A+M+G GR +G+EHI ELV
Sbjct: 63 TISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPELVA 122
Query: 438 LATKNIQNDNPSLLSSER 491
+ KNI+ S ER
Sbjct: 123 SSVKNIEASAASPFLKER 140
Score = 83.8 bits (198), Expect = 1e-16
Identities = 42/70 (60%), Positives = 50/70 (71%)
Frame = +2
Query: 509 DGRLGYPSEAPYSAIHVGAAAPTLPQALIDQLKPGGRLIVPVGPEGGEQHLTQVDKAQDG 688
DGR G+ APY AIHVGAAAP +P+ALIDQLKPGGRL++PVG Q L VDK DG
Sbjct: 148 DGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLKPGGRLVIPVG--NIFQDLQVVDKNSDG 205
Query: 689 TTTVKKLMSV 718
+ ++K SV
Sbjct: 206 SVSIKDETSV 215
Score = 46.4 bits (105), Expect = 2e-05
Identities = 25/55 (45%), Positives = 34/55 (61%), Gaps = 2/55 (3%)
Frame = +1
Query: 115 NVDLIRNLRTNGIIKSDTVANAMLAVDRKNYCP--SSPYQDSPQSIGFSQQSALP 273
N ++ NL+ +GI+ SD VA AM AVDR + SS Y DSP SIG++ + P
Sbjct: 13 NKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGYNVTISAP 67
>At5g50240.1 68418.m06222 protein-L-isoaspartate
O-methyltransferase, putative / PIMT, putative similar
to SP|Q42539 Protein-L-isoaspartate O-methyltransferase
(EC 2.1.1.77) (Protein- beta-aspartate
methyltransferase) (PIMT) (Protein L-isoaspartyl
methyltransferase) (L-isoaspartyl protein carboxyl
methyltransferase) {Arabidopsis thaliana}; contains Pfam
profile PF01135: Protein-L-isoaspartate(D-aspartate)
O-methyltransferase (PCMT)
Length = 269
Score = 74.9 bits (176), Expect = 6e-14
Identities = 37/70 (52%), Positives = 47/70 (67%)
Frame = +2
Query: 509 DGRLGYPSEAPYSAIHVGAAAPTLPQALIDQLKPGGRLIVPVGPEGGEQHLTQVDKAQDG 688
DGR G+ APY AIHVGAAA +PQ L+DQLKPGGR+++P+G Q L +DK +DG
Sbjct: 187 DGRKGWQEFAPYDAIHVGAAASEIPQPLLDQLKPGGRMVIPLGTY--FQELKVIDKNEDG 244
Query: 689 TTTVKKLMSV 718
+ V SV
Sbjct: 245 SIKVHTETSV 254
Score = 57.2 bits (132), Expect = 1e-08
Identities = 27/55 (49%), Positives = 37/55 (67%), Gaps = 1/55 (1%)
Frame = +3
Query: 348 GSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQND-NPSLLSSERIKLVVG 509
G+GYLT C A+M+G GRVVG++HI ELV+++ KNI+ S L + L VG
Sbjct: 132 GTGYLTGCFALMVGAEGRVVGVDHIPELVDMSIKNIEKSVAASFLKKGSLSLHVG 186
>At1g73320.1 68414.m08485 expressed protein
Length = 292
Score = 32.3 bits (70), Expect = 0.40
Identities = 24/81 (29%), Positives = 44/81 (54%), Gaps = 1/81 (1%)
Frame = +3
Query: 225 PGLS-SIYWILATISAPHMHAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGR 401
PG++ S+ W + + H+++ K + G+K +++GSG G L C+A +LG G
Sbjct: 91 PGVTGSVMWDSGVVLGKFLE-HSVDS-KVLSLEGKKIVELGSGCG-LVGCIAALLG--GN 145
Query: 402 VVGIEHISELVNLATKNIQND 464
V + + + + L KNIQ +
Sbjct: 146 AV-LTDLPDRLRLLKKNIQTN 165
>At1g23360.1 68414.m02923 UbiE/COQ5 methyltransferase family protein
similar to 2-hexaprenyl-1,4-naphthoquinone
methyltransferase GB:BAA25267 GI:2982680 from
[Micrococcus luteus]; contains Pfam profile PF01209:
methlytransferase, UbiE/COQ5 family
Length = 198
Score = 32.3 bits (70), Expect = 0.40
Identities = 15/41 (36%), Positives = 26/41 (63%)
Frame = +3
Query: 321 GEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLA 443
G+ LD+ GSG L ++ +G TG+V+G++ SE + +A
Sbjct: 11 GDYVLDLCCGSGDLAFLLSEKVGSTGKVMGLDFSSEQLAVA 51
>At5g52490.1 68418.m06512 fibrillarin, putative similar to
fibrillarin from {Xenopus laevis} SP|P22232, {Mus
musculus} SP|P35550, {Homo sapiens} SP|P22087
Length = 292
Score = 31.9 bits (69), Expect = 0.53
Identities = 22/72 (30%), Positives = 37/72 (51%), Gaps = 6/72 (8%)
Frame = +3
Query: 318 PGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHI----SELVNLATK--NIQNDNPSLL 479
PG K L +G+ SGY + ++ ++G G V +EH L+N+A K N+
Sbjct: 133 PGVKVLYLGASSGYTVSHVSDIVGPEGCVYAVEHSDICGKVLMNMAEKRTNVIPIIEDAR 192
Query: 480 SSERIKLVVGMV 515
+ +++VGMV
Sbjct: 193 HPAKYRMLVGMV 204
>At3g62000.1 68416.m06963 O-methyltransferase family 3 protein
several O-methyltransferases - different species;
contains Pfam 01596 O-methyltransferase domain
Length = 278
Score = 31.9 bits (69), Expect = 0.53
Identities = 14/47 (29%), Positives = 29/47 (61%)
Frame = +3
Query: 309 QLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATK 449
Q++ E+ ++VG +GY + +A++L E+G +V E S + +A +
Sbjct: 115 QMLAAERCIEVGVYTGYSSLAVALVLPESGCLVACERDSNSLEVAKR 161
>At5g14600.1 68418.m01712 expressed protein
Length = 318
Score = 31.5 bits (68), Expect = 0.71
Identities = 17/67 (25%), Positives = 33/67 (49%)
Frame = +3
Query: 309 QLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQNDNPSLLSSE 488
++VPG L+ G+GSG L+ +A + TG V + + A ++ + S L +
Sbjct: 105 EVVPGCVVLESGTGSGSLSTSLARAVAPTGHVYSFDFHEQRAVSAREDFEKTGISSLVTV 164
Query: 489 RIKLVVG 509
++ + G
Sbjct: 165 EVRDIQG 171
>At5g64150.1 68418.m08055 methylase family protein contains TIGRfam
TIGR00536: modification methylase, HemK family
Length = 377
Score = 31.1 bits (67), Expect = 0.93
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = +3
Query: 336 DVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQ 458
D+G+GSG + +A +LG GRV+ + + +A N+Q
Sbjct: 206 DLGTGSGAIAIGIAKVLGSRGRVIATDLSPVAIAVAGHNVQ 246
>At1g17145.1 68414.m02090 expressed protein
Length = 335
Score = 31.1 bits (67), Expect = 0.93
Identities = 17/41 (41%), Positives = 26/41 (63%)
Frame = +3
Query: 345 SGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQNDN 467
S SG L AC +L E ++VG+ ++ VNLA++N+ DN
Sbjct: 252 SPSGGL-ACAIAVLAERQQMVGVSSSNQNVNLASQNLVPDN 291
>At5g49020.2 68418.m06066 protein arginine N-methyltransferase
family protein similar to protein arginine
methyltransferase [Mus musculus] GI:5257221
Length = 526
Score = 29.9 bits (64), Expect = 2.2
Identities = 27/82 (32%), Positives = 39/82 (47%)
Frame = +3
Query: 279 HAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQ 458
HA +E + G +DVG+GSG L+ M L V +E SE+ A K I
Sbjct: 173 HAAVMENRSD--FSGRVVVDVGAGSGILS--MFAALAGAKHVYAVE-ASEMAEYARKLIA 227
Query: 459 NDNPSLLSSERIKLVVGMVVSV 524
NP L +ERI ++ G + +
Sbjct: 228 G-NP--LLAERITVIKGKIEDI 246
>At5g49020.1 68418.m06065 protein arginine N-methyltransferase
family protein similar to protein arginine
methyltransferase [Mus musculus] GI:5257221
Length = 528
Score = 29.9 bits (64), Expect = 2.2
Identities = 27/82 (32%), Positives = 39/82 (47%)
Frame = +3
Query: 279 HAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQ 458
HA +E + G +DVG+GSG L+ M L V +E SE+ A K I
Sbjct: 175 HAAVMENRSD--FSGRVVVDVGAGSGILS--MFAALAGAKHVYAVE-ASEMAEYARKLIA 229
Query: 459 NDNPSLLSSERIKLVVGMVVSV 524
NP L +ERI ++ G + +
Sbjct: 230 G-NP--LLAERITVIKGKIEDI 248
>At5g15540.1 68418.m01819 expressed protein low similarity to DNA
repair and meiosis protein Rad9 [Coprinus cinereus]
GI:1353390, SP|Q09725 Sister chromatid cohesion protein
mis4 {Schizosaccharomyces pombe}
Length = 1755
Score = 29.5 bits (63), Expect = 2.8
Identities = 19/63 (30%), Positives = 33/63 (52%), Gaps = 2/63 (3%)
Frame = +3
Query: 318 PGEKAL--DVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATKNIQNDNPSLLSSER 491
PGE L +VG GSGY + + +L ++ R+ + H +++ L +N + P S E
Sbjct: 48 PGEFKLFDEVGQGSGYRSLDRSEILAQSSRIANMLHETDVSYLDLRN-EARAPDCNSGEH 106
Query: 492 IKL 500
+L
Sbjct: 107 FQL 109
>At3g61990.1 68416.m06962 O-methyltransferase family 3 protein
several O-methyltransferases - different species;
contains Pfam 01596 O-methyltransferase domain
Length = 290
Score = 29.5 bits (63), Expect = 2.8
Identities = 11/47 (23%), Positives = 30/47 (63%)
Frame = +3
Query: 309 QLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNLATK 449
+++ ++ ++VG +GY + +A++L E+GR+V + + + +A +
Sbjct: 127 EILGAKRCIEVGVYTGYSSLAVALVLPESGRLVACDKDANALEVAKR 173
>At5g54400.1 68418.m06775 expressed protein
Length = 292
Score = 29.1 bits (62), Expect = 3.8
Identities = 19/48 (39%), Positives = 27/48 (56%), Gaps = 5/48 (10%)
Frame = +3
Query: 330 ALDVGSGSGYLTACMAMML---GETGRVVGIEH--ISELVNLATKNIQ 458
ALD+G G G L +A L G +GRVVG++ + L L T +I+
Sbjct: 118 ALDLGCGRGILLNAVATQLKKTGSSGRVVGLDRSMTTTLSTLRTAHIE 165
>At4g26590.1 68417.m03833 oligopeptide transporter OPT family
protein similar to SP|P40900 Sexual differentiation
process protein isp4 {Schizosaccharomyces pombe};
contains Pfam profile PF03169: OPT oligopeptide
transporter protein
Length = 753
Score = 29.1 bits (62), Expect = 3.8
Identities = 22/76 (28%), Positives = 33/76 (43%), Gaps = 6/76 (7%)
Frame = -2
Query: 511 IPTTSLILSDESKLGLSFWMFL-VAKFTSSDIC--SIPTTLP---VSPNIMAIQAVR*PD 350
+ + I++ G ++W+ V ++D+ S P T P V N I + P
Sbjct: 546 VQLVATIVASTVSFGTTWWLLSSVENICNTDMLPKSSPWTCPGDVVFYNASIIWGIIGPG 605
Query: 349 PEPTSKAFSPGTNWFF 302
TSK PG NWFF
Sbjct: 606 RMFTSKGIYPGMNWFF 621
>At3g15530.2 68416.m01969 expressed protein
Length = 288
Score = 29.1 bits (62), Expect = 3.8
Identities = 18/42 (42%), Positives = 25/42 (59%), Gaps = 3/42 (7%)
Frame = +3
Query: 330 ALDVGSGSGYLTACMAMML---GETGRVVGIEHISELVNLAT 446
ALD+G G G L +A L G +GRVVG++ S+ L+T
Sbjct: 115 ALDLGCGRGILLNAVATQLKKTGSSGRVVGLDR-SKRTTLST 155
>At3g15530.1 68416.m01968 expressed protein
Length = 288
Score = 29.1 bits (62), Expect = 3.8
Identities = 18/42 (42%), Positives = 25/42 (59%), Gaps = 3/42 (7%)
Frame = +3
Query: 330 ALDVGSGSGYLTACMAMML---GETGRVVGIEHISELVNLAT 446
ALD+G G G L +A L G +GRVVG++ S+ L+T
Sbjct: 115 ALDLGCGRGILLNAVATQLKKTGSSGRVVGLDR-SKRTTLST 155
>At4g26220.1 68417.m03775 caffeoyl-CoA 3-O-methyltransferase,
putative similar to caffeoyl-CoA O-methyltransferase
[GI:1622926][Nicotiana tabacum], GI:2960356 [Populus
balsamifera subsp. trichocarpa], AF036095 [Pinus taeda]
Length = 232
Score = 28.3 bits (60), Expect = 6.6
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +3
Query: 312 LVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIE 416
LV K ++VG +GY A+ L E G+V+ I+
Sbjct: 63 LVNARKTIEVGVFTGYSLLLTALTLPEDGKVIAID 97
>At5g59990.1 68418.m07523 expressed protein ; expression supported
by MPSS
Length = 241
Score = 27.9 bits (59), Expect = 8.7
Identities = 12/18 (66%), Positives = 15/18 (83%)
Frame = -2
Query: 757 DDIGAPCQLMEHNHTHQF 704
DDI AP QL++H+H HQF
Sbjct: 22 DDIVAPPQLLDHHH-HQF 38
>At1g24735.1 68414.m03105 caffeoyl-CoA 3-O-methyltransferase,
putative similar to SP|Q43237 [Vitis vinifera],
GI:684942 [Medicago sativa subsp. sativa]
Length = 195
Score = 27.9 bits (59), Expect = 8.7
Identities = 17/67 (25%), Positives = 31/67 (46%)
Frame = +3
Query: 261 ISAPHMHAHALEKLKNQLVPGEKALDVGSGSGYLTACMAMMLGETGRVVGIEHISELVNL 440
+ P H L L +++ +K +++G +GY A+ L G V GI+ E +
Sbjct: 11 MEVPVDEGHFLSMLL-KIMNAKKTIELGVFTGYSLLTTALALPHDGHVTGIDIDKEAYEM 69
Query: 441 ATKNIQN 461
+ I+N
Sbjct: 70 GLEFIKN 76
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,593,415
Number of Sequences: 28952
Number of extensions: 414026
Number of successful extensions: 1143
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 1083
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1138
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1902108000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -