BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40165
(762 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 38 0.001
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 35 0.011
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 28 1.3
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 27 2.2
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ... 27 2.2
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 27 2.9
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ... 26 5.1
SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog... 26 6.7
SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3... 26 6.7
SPAC1002.16c |||nicotinic acid plasma membrane transporter |Schi... 25 8.9
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 38.3 bits (85), Expect = 0.001
Identities = 21/71 (29%), Positives = 41/71 (57%)
Frame = +2
Query: 266 QARVWQWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTF 445
+A+V Q+ S+++SEL A + P + +++Q + A++ + D L ++T+
Sbjct: 90 KAKVLQYCSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTY 149
Query: 446 LVTERITLADV 478
LV R+TLAD+
Sbjct: 150 LVGSRLTLADI 160
Score = 31.9 bits (69), Expect = 0.10
Identities = 17/39 (43%), Positives = 23/39 (58%)
Frame = +3
Query: 120 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN 236
N D KFP K+P F DG L+E+ AIA+Y+A+
Sbjct: 38 NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLAS 75
>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 220
Score = 35.1 bits (77), Expect = 0.011
Identities = 22/87 (25%), Positives = 48/87 (55%), Gaps = 2/87 (2%)
Frame = +2
Query: 254 NLATQARVWQWASWSDSELL-PASCA-WVFPYLGIMQFNKQNVERAKSDLLAALKVLDGH 427
N +A + +W + + +++ P + WV + G + + ++ + + + + +LK+ +
Sbjct: 89 NEVEEAEMLKWMCFINFDIVTPQNVRPWVGMFRGNIPYEEKPFKESATRAIDSLKIPNEL 148
Query: 428 LLTRTFLVTERITLADVIVFSTLLHAF 508
+ RT+LV +R TLAD + F +LL F
Sbjct: 149 VKDRTYLVGDRFTLAD-LFFGSLLRIF 174
Score = 30.3 bits (65), Expect = 0.31
Identities = 24/73 (32%), Positives = 31/73 (42%)
Frame = +3
Query: 9 GVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADG 188
G LY++ N R L A+ V + + S D KFP K+P F ADG
Sbjct: 4 GTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIGADG 60
Query: 189 KVLLTESNAIAYY 227
L+E AI Y
Sbjct: 61 -FELSEVIAIVKY 72
>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 442
Score = 28.3 bits (60), Expect = 1.3
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +3
Query: 393 TYWPP*KYWTDIFSHAPSLLPRESHLPMSLS 485
TY PP Y D+FS APS+ + S+S
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMS 287
>SPBC211.03c |||guanyl-nucleotide exchange
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1462
Score = 27.5 bits (58), Expect = 2.2
Identities = 20/54 (37%), Positives = 24/54 (44%), Gaps = 5/54 (9%)
Frame = +3
Query: 435 HAPSLLPRESHLPMSLSSVHCCM-LSARARPER----PFVADKRSALVPDRRPP 581
H PS PRE ++ SL V + LS P+R F KR A RPP
Sbjct: 391 HIPSTTPRERNVETSLHKVVSSLDLSEEISPDRATPTSFTERKRFAFDTRNRPP 444
>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 267
Score = 27.5 bits (58), Expect = 2.2
Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
Frame = +3
Query: 255 IWLPKPVSGSGHHGLTVNYCL-LPVLGSSLTLV 350
+W+P PV SG T+ C+ LP G +T V
Sbjct: 23 LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55
>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
Eme1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 27.1 bits (57), Expect = 2.9
Identities = 16/62 (25%), Positives = 30/62 (48%)
Frame = +3
Query: 27 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 206
P F++ KA + QY+ +FG +K ++F K+ ++ F S + +L+
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611
Query: 207 SN 212
SN
Sbjct: 612 SN 613
>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 369
Score = 26.2 bits (55), Expect = 5.1
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -3
Query: 196 STFPSALSNAGTFPAGNFFKKSSDL 122
+TFP A + GTFP G F S L
Sbjct: 52 ATFPWAQAPVGTFPIGQLFDPVSGL 76
>SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 982
Score = 25.8 bits (54), Expect = 6.7
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +3
Query: 303 VNYCLLPVLGSSLTLVSCNSTNRML 377
V + L+P LG L + SCN+ R L
Sbjct: 259 VTHALVPELGLQLAMASCNALLRYL 283
>SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 378
Score = 25.8 bits (54), Expect = 6.7
Identities = 17/74 (22%), Positives = 29/74 (39%)
Frame = +3
Query: 108 FGETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVANESLRGGIWLPKPVSGSG 287
FG + K + G F D ++L + ++ S+ GG+ L +SG+
Sbjct: 55 FGHSRKGGPKKQGCTGGWSLVFPDLDYQILRASDTELPLFLWGHSMGGGLALRYGISGTH 114
Query: 288 HHGLTVNYCLLPVL 329
H L P+L
Sbjct: 115 RHKLAGVIAQAPML 128
>SPAC1002.16c |||nicotinic acid plasma membrane transporter
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 25.4 bits (53), Expect = 8.9
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +3
Query: 258 WLPKPVSGSGHHGLTVNYCLLPV 326
+LP +SG G+ L+V Y +PV
Sbjct: 308 FLPAIISGMGYTSLSVQYMTIPV 330
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,129,062
Number of Sequences: 5004
Number of extensions: 67300
Number of successful extensions: 208
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 208
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 365309308
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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