Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40162
(658 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g47470.2 68415.m05924 thioredoxin family protein similar to p... 36 0.018
At2g47470.1 68415.m05925 thioredoxin family protein similar to p... 36 0.018
At1g77510.1 68414.m09026 protein disulfide isomerase, putative s... 36 0.024
At5g60640.2 68418.m07611 thioredoxin family protein similar to p... 35 0.041
At5g60640.1 68418.m07610 thioredoxin family protein similar to p... 35 0.041
At3g54960.1 68416.m06094 thioredoxin family protein similar to p... 35 0.055
At1g21750.2 68414.m02723 protein disulfide isomerase, putative s... 33 0.13
At1g21750.1 68414.m02722 protein disulfide isomerase, putative s... 33 0.13
At1g07960.3 68414.m00867 thioredoxin family protein low similari... 31 0.89
At1g07960.2 68414.m00866 thioredoxin family protein low similari... 31 0.89
At1g07960.1 68414.m00865 thioredoxin family protein low similari... 31 0.89
At3g18480.1 68416.m02348 CCAAT displacement protein-related / CD... 30 1.6
At2g26700.1 68415.m03203 protein kinase family protein contains ... 29 2.1
At5g63720.1 68418.m07998 hypothetical protein 29 3.6
At5g18740.1 68418.m02224 expressed protein predicted proteins - ... 28 4.8
At4g38550.1 68417.m05458 expressed protein 24 5.4
At1g52990.1 68414.m05997 thioredoxin family protein similar to S... 27 8.3
>At2g47470.2 68415.m05924 thioredoxin family protein similar to
protein disulfide isomerase [Dictyostelium discoideum]
GI:2627440; contains Pfam profile: PF00085 Thioredoxin
Length = 266
Score = 36.3 bits (80), Expect = 0.018
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDVSLPK 267
CGHCK+L P Y+KLG F+ V + K
Sbjct: 52 CGHCKKLAPEYEKLGASFKKAKSVLIAK 79
Score = 35.5 bits (78), Expect = 0.031
Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = +3
Query: 303 ITSFPTIKLYSKDNQV-HDYNGERTLAGLTKFV 398
++ FPT+K + KDN+ HDY+G R L F+
Sbjct: 213 VSGFPTLKFFPKDNKAGHDYDGGRDLDDFVSFI 245
Score = 33.1 bits (72), Expect = 0.17
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDV 255
CGHCK L P Y+K+ F+ ++ V
Sbjct: 171 CGHCKSLAPTYEKVATVFKQEEGV 194
>At2g47470.1 68415.m05925 thioredoxin family protein similar to
protein disulfide isomerase [Dictyostelium discoideum]
GI:2627440; contains Pfam profile: PF00085 Thioredoxin
Length = 361
Score = 36.3 bits (80), Expect = 0.018
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDVSLPK 267
CGHCK+L P Y+KLG F+ V + K
Sbjct: 52 CGHCKKLAPEYEKLGASFKKAKSVLIAK 79
Score = 35.5 bits (78), Expect = 0.031
Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = +3
Query: 303 ITSFPTIKLYSKDNQV-HDYNGERTLAGLTKFV 398
++ FPT+K + KDN+ HDY+G R L F+
Sbjct: 213 VSGFPTLKFFPKDNKAGHDYDGGRDLDDFVSFI 245
Score = 33.1 bits (72), Expect = 0.17
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDV 255
CGHCK L P Y+K+ F+ ++ V
Sbjct: 171 CGHCKSLAPTYEKVATVFKQEEGV 194
>At1g77510.1 68414.m09026 protein disulfide isomerase, putative
similar to protein disulfide isomerase precursor
GB:P29828 GI:4704766 [Medicago sativa]; Pfam HMM hit:
PF00085 Thioredoxins
Length = 508
Score = 35.9 bits (79), Expect = 0.024
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDVSLPK 267
CGHC++L PI D++ F+ND V + K
Sbjct: 402 CGHCQKLAPILDEVALSFQNDPSVIIAK 429
Score = 31.9 bits (69), Expect = 0.39
Identities = 23/66 (34%), Positives = 30/66 (45%), Gaps = 6/66 (9%)
Frame = +3
Query: 303 ITSFPTIKLYSKDNQVHDYNGERTLAGLTKFVETDGE------GAEPVPSVTEFEEEEDV 464
+ FPTI S V Y G+RT FVE + E G E S E ++ E+
Sbjct: 444 VKGFPTIYFRSASGNVVVYEGDRTKEDFINFVEKNSEKKPTSHGEESTKS-EEPKKTEET 502
Query: 465 PARDEL 482
A+DEL
Sbjct: 503 AAKDEL 508
Score = 27.9 bits (59), Expect = 6.3
Identities = 12/26 (46%), Positives = 18/26 (69%), Gaps = 3/26 (11%)
Frame = +1
Query: 154 QHEHFVL-FYR--CGHCKQLVPIYDK 222
+H+ V+ FY CGHC++L P Y+K
Sbjct: 45 KHDFIVVEFYAPWCGHCQKLAPEYEK 70
>At5g60640.2 68418.m07611 thioredoxin family protein similar to
protein disulfide isomerase GI:5902592 from [Volvox
carteri f. nagariensis], GI:2708314 from Chlamydomonas
reinhardtii; contains Pfam profile: PF00085 Thioredoxin
Length = 536
Score = 35.1 bits (77), Expect = 0.041
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDVSLPK 267
CGHC+ L P+Y+KL +H + D + + K
Sbjct: 471 CGHCQALEPMYNKLAKHLRSIDSLVITK 498
>At5g60640.1 68418.m07610 thioredoxin family protein similar to
protein disulfide isomerase GI:5902592 from [Volvox
carteri f. nagariensis], GI:2708314 from Chlamydomonas
reinhardtii; contains Pfam profile: PF00085 Thioredoxin
Length = 597
Score = 35.1 bits (77), Expect = 0.041
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDVSLPK 267
CGHC+ L P+Y+KL +H + D + + K
Sbjct: 471 CGHCQALEPMYNKLAKHLRSIDSLVITK 498
>At3g54960.1 68416.m06094 thioredoxin family protein similar to
protein disulfide isomerase GI:5902592 from [Volvox
carteri f. nagariensis], GI:2708314 from Chlamydomonas
reinhardtii; contains Pfam profile: PF00085 Thioredoxin
Length = 579
Score = 34.7 bits (76), Expect = 0.055
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDVSLPK 267
CGHC+ PIY+KLG++ + D + + K
Sbjct: 467 CGHCQSFEPIYNKLGKYLKGIDSLVVAK 494
Score = 29.1 bits (62), Expect = 2.7
Identities = 16/55 (29%), Positives = 28/55 (50%)
Frame = +3
Query: 300 KITSFPTIKLYSKDNQVHDYNGERTLAGLTKFVETDGEGAEPVPSVTEFEEEEDV 464
+I FPT+ L+ Y GERT G+ +++ + + + ++T EE E V
Sbjct: 167 EIQGFPTVFLFVDGEMRKTYEGERTKDGIVTWLKK--KASPSIHNITTKEEAERV 219
>At1g21750.2 68414.m02723 protein disulfide isomerase, putative
similar to SP|P29828 Protein disulfide isomerase
precursor (PDI) (EC 5.3.4.1) {Medicago sativa}; isoform
contains non-consensus GA donor splice site at intron 9
Length = 487
Score = 33.5 bits (73), Expect = 0.13
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDVSLPK 267
CGHC++L PI D++ +++D V + K
Sbjct: 404 CGHCQKLAPILDEVAVSYQSDSSVVIAK 431
Score = 31.1 bits (67), Expect = 0.67
Identities = 14/26 (53%), Positives = 18/26 (69%), Gaps = 3/26 (11%)
Frame = +1
Query: 154 QHEHFVL-FYR--CGHCKQLVPIYDK 222
+H+ V+ FY CGHCKQL P Y+K
Sbjct: 46 KHDFIVVEFYAPWCGHCKQLAPEYEK 71
>At1g21750.1 68414.m02722 protein disulfide isomerase, putative
similar to SP|P29828 Protein disulfide isomerase
precursor (PDI) (EC 5.3.4.1) {Medicago sativa}; isoform
contains non-consensus GA donor splice site at intron 9
Length = 501
Score = 33.5 bits (73), Expect = 0.13
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDVSLPK 267
CGHC++L PI D++ +++D V + K
Sbjct: 404 CGHCQKLAPILDEVAVSYQSDSSVVIAK 431
Score = 31.1 bits (67), Expect = 0.67
Identities = 14/26 (53%), Positives = 18/26 (69%), Gaps = 3/26 (11%)
Frame = +1
Query: 154 QHEHFVL-FYR--CGHCKQLVPIYDK 222
+H+ V+ FY CGHCKQL P Y+K
Sbjct: 46 KHDFIVVEFYAPWCGHCKQLAPEYEK 71
Score = 27.5 bits (58), Expect = 8.3
Identities = 17/54 (31%), Positives = 23/54 (42%), Gaps = 1/54 (1%)
Frame = +3
Query: 303 ITSFPTIKLYSKDNQVHDYNGERTLAGLTKFVETDGEG-AEPVPSVTEFEEEED 461
+ FPTI S V Y G+RT FV+ + + EP EE +D
Sbjct: 446 VKGFPTIYFKSASGNVVVYEGDRTKEDFISFVDKNKDTVGEPKKEEETTEEVKD 499
>At1g07960.3 68414.m00867 thioredoxin family protein low similarity
to protein disulfide isomerase 4 [Giardia intestinalis]
GI:13489047; contains Pfam profile PF00085: Thioredoxin
Length = 146
Score = 30.7 bits (66), Expect = 0.89
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDVSL 261
C HCK+L +++ LG+ E DD++ +
Sbjct: 55 CKHCKKLGNLWEDLGKAMEGDDEIEV 80
Score = 29.5 bits (63), Expect = 2.1
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +3
Query: 300 KITSFPTIKLYSKDNQVHDYNGERTLAGLTKFVETDGEGA 419
+I S+PT L+ +V Y G+R + L FV + E A
Sbjct: 96 EIHSYPTFMLFYNGEEVSKYKGKRDVESLKAFVVEETEKA 135
>At1g07960.2 68414.m00866 thioredoxin family protein low similarity
to protein disulfide isomerase 4 [Giardia intestinalis]
GI:13489047; contains Pfam profile PF00085: Thioredoxin
Length = 146
Score = 30.7 bits (66), Expect = 0.89
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDVSL 261
C HCK+L +++ LG+ E DD++ +
Sbjct: 55 CKHCKKLGNLWEDLGKAMEGDDEIEV 80
Score = 29.5 bits (63), Expect = 2.1
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +3
Query: 300 KITSFPTIKLYSKDNQVHDYNGERTLAGLTKFVETDGEGA 419
+I S+PT L+ +V Y G+R + L FV + E A
Sbjct: 96 EIHSYPTFMLFYNGEEVSKYKGKRDVESLKAFVVEETEKA 135
>At1g07960.1 68414.m00865 thioredoxin family protein low similarity
to protein disulfide isomerase 4 [Giardia intestinalis]
GI:13489047; contains Pfam profile PF00085: Thioredoxin
Length = 146
Score = 30.7 bits (66), Expect = 0.89
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFENDDDVSL 261
C HCK+L +++ LG+ E DD++ +
Sbjct: 55 CKHCKKLGNLWEDLGKAMEGDDEIEV 80
Score = 29.5 bits (63), Expect = 2.1
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +3
Query: 300 KITSFPTIKLYSKDNQVHDYNGERTLAGLTKFVETDGEGA 419
+I S+PT L+ +V Y G+R + L FV + E A
Sbjct: 96 EIHSYPTFMLFYNGEEVSKYKGKRDVESLKAFVVEETEKA 135
>At3g18480.1 68416.m02348 CCAAT displacement protein-related /
CDP-related similar to CCAAT displacement protein (CDP)
(Cut-like 1) (Swiss-Prot:P39880) [Homo sapiens];
contains Pfam:PF00904 Involucrin repeat
Length = 689
Score = 29.9 bits (64), Expect = 1.6
Identities = 17/41 (41%), Positives = 25/41 (60%), Gaps = 1/41 (2%)
Frame = +3
Query: 282 NELEHTKITSFPTIKLYSKDNQVHDYNGERTLA-GLTKFVE 401
+ELE TK + +KLY K V DYN ++ ++ G K+VE
Sbjct: 544 DELEKTKADN---VKLYGKIRYVQDYNHDKVVSRGSKKYVE 581
>At2g26700.1 68415.m03203 protein kinase family protein contains
Pfam PF00069: Protein kinase domain
Length = 525
Score = 29.5 bits (63), Expect = 2.1
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +1
Query: 166 FVLFYRCGHCKQLVPIYDKLGEHFENDDDVSLPKSTPPPTNWNTP 300
F L ++C Q + D+ H E+DDD+S+ + P+ TP
Sbjct: 233 FDLSFKCDVVPQFLSDNDRDRGHQEDDDDISIRRKCSTPSCTTTP 277
>At5g63720.1 68418.m07998 hypothetical protein
Length = 492
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +1
Query: 238 ENDDDVSLPKSTPPPTNWNTPRSRPSQRSN 327
+NDD VS ++PP T+ +T R QR N
Sbjct: 323 QNDDSVSEVSTSPPHTDRDTSREPGKQRRN 352
>At5g18740.1 68418.m02224 expressed protein predicted proteins -
Arabidopsis thaliana; expression supported by MPSS
Length = 609
Score = 28.3 bits (60), Expect = 4.8
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +3
Query: 339 DNQVHDYNGERTLAGLTKFVETDGEGAEPVPSVTEFE 449
D+ D NG R T++ + EG+EP+ ++ + E
Sbjct: 534 DDDDDDENGNRLFRACTEYTYNEAEGSEPIFTIPKSE 570
>At4g38550.1 68417.m05458 expressed protein
Length = 612
Score = 23.8 bits (49), Expect(2) = 5.4
Identities = 8/21 (38%), Positives = 15/21 (71%)
Frame = +1
Query: 217 DKLGEHFENDDDVSLPKSTPP 279
+++ + +E DDDV+ S+PP
Sbjct: 259 EQIEDFYEQDDDVTPRNSSPP 279
Score = 22.6 bits (46), Expect(2) = 5.4
Identities = 9/20 (45%), Positives = 11/20 (55%)
Frame = +1
Query: 268 STPPPTNWNTPRSRPSQRSN 327
S PPP + TP R S + N
Sbjct: 289 SPPPPQPYRTPDHRRSHQDN 308
>At1g52990.1 68414.m05997 thioredoxin family protein similar to
SP|P48384 Thioredoxin M-type, chloroplast precursor
(TRX-M) {Pisum sativum}; contains Pfam profile PF00085:
Thioredoxin
Length = 313
Score = 27.5 bits (58), Expect = 8.3
Identities = 7/21 (33%), Positives = 15/21 (71%)
Frame = +1
Query: 184 CGHCKQLVPIYDKLGEHFEND 246
CG C+ ++PI +K+ ++N+
Sbjct: 239 CGPCRDMIPILNKMDSEYKNE 259
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,924,824
Number of Sequences: 28952
Number of extensions: 220547
Number of successful extensions: 760
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 717
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 760
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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