Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdV40127
         (444 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosac...    85   6e-18
SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Sc...    79   3e-16
SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S...    28   0.74 
SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharom...    27   0.98 
SPCC306.11 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||...    27   0.98 
SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pomb...    27   1.7  
SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces p...    26   3.0  
SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19 |Schizosa...    26   3.0  
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc...    25   5.2  
SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ...    25   6.9  
SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi...    25   6.9  
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce...    25   6.9  
SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomy...    25   6.9  
SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac...    25   6.9  

>SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein
           S24|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 134

 Score = 84.6 bits (200), Expect = 6e-18
 Identities = 37/58 (63%), Positives = 46/58 (79%)
 Frame = +1

Query: 76  MVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFGFKTNFGGGKSTGFALIYDTLD 249
           MV D+LHPGK  +SK EIREKLA+MYK   + V  FG +T+FGGG+STGFALIYD+ +
Sbjct: 23  MVVDILHPGKANLSKNEIREKLAQMYKTDSECVQAFGLRTHFGGGRSTGFALIYDSTE 80



 Score = 68.9 bits (161), Expect = 3e-13
 Identities = 49/122 (40%), Positives = 61/122 (50%), Gaps = 1/122 (0%)
 Frame = +3

Query: 6   MSEGTATIRTRKFMTNRLLARKQHGLRCFTSRKTNRQQDRDP*EARQNVQGYSRCSVRIR 185
           MSE   TIRTRKFMTNRLL RKQ  +      K N  ++    +  Q  +  S C     
Sbjct: 1   MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANLSKNEIREKLAQMYKTDSECVQAFG 59

Query: 186 FQDKLRRWQVNWIRFDLRHTRSGKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVR 362
            +      +          T S KKFEP +RL R G  E  ++  R+QRK+RKNR KKV 
Sbjct: 60  LRTHFGGGRSTGFALIYDSTESMKKFEPHYRLVRVGQAEPIQKVARQQRKQRKNRGKKVF 119

Query: 363 GT 368
           GT
Sbjct: 120 GT 121


>SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein
           S24|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 134

 Score = 79.0 bits (186), Expect = 3e-16
 Identities = 32/58 (55%), Positives = 44/58 (75%)
 Frame = +1

Query: 76  MVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFGFKTNFGGGKSTGFALIYDTLD 249
           MV D+LHPGK  +SK ++REKL +MYK     V  FG +T++GGG++TGFALIYD ++
Sbjct: 23  MVVDILHPGKANISKNDLREKLGQMYKTDASAVQAFGLRTHYGGGRTTGFALIYDDVE 80



 Score = 58.4 bits (135), Expect = 5e-10
 Identities = 45/122 (36%), Positives = 59/122 (48%), Gaps = 1/122 (0%)
 Frame = +3

Query: 6   MSEGTATIRTRKFMTNRLLARKQHGLRCFTSRKTNRQQDRDP*EARQNVQGYSRCSVRIR 185
           MSE   TIRTRKFMTNRLL RKQ  +      K N  ++    +  Q  +  +       
Sbjct: 1   MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANISKNDLREKLGQMYKTDASAVQAFG 59

Query: 186 FQDKLRRWQVNWIRFDLRHTRSGKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVR 362
            +      +            + KKFEP +RL R G  E  ++  R+QRK+RKNR KKV 
Sbjct: 60  LRTHYGGGRTTGFALIYDDVEAMKKFEPHYRLVRVGHAEPIQKVARQQRKQRKNRGKKVF 119

Query: 363 GT 368
           GT
Sbjct: 120 GT 121


>SPCC417.06c |ppk35|mug27|serine/threonine protein kinase
           Ppk35|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 624

 Score = 27.9 bits (59), Expect = 0.74
 Identities = 12/33 (36%), Positives = 18/33 (54%)
 Frame = +3

Query: 258 KFEPKHRLARHGLYEKKRPTRKQRKERKNRMKK 356
           K+ P  R    G + +KR  RK++ E+KN   K
Sbjct: 539 KYRPNARKPLVGRHREKRQLRKEKPEKKNNSTK 571


>SPAC22A12.11 |dak1||dihydroxyacetone kinase
           Dak1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 580

 Score = 27.5 bits (58), Expect = 0.98
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +1

Query: 124 EIREKLAKMYKVTPDVVFVFGFKTNFGG 207
           +++E LAK YK+ P  +F   F T+  G
Sbjct: 286 KVKEALAKEYKINPVRIFAGPFTTSLNG 313


>SPCC306.11 |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 283

 Score = 27.5 bits (58), Expect = 0.98
 Identities = 16/51 (31%), Positives = 25/51 (49%), Gaps = 3/51 (5%)
 Frame = -2

Query: 155 LYILASFSRISVLLTVGFPG---CKTSQTMLLARQQSVGHELASANSRCSF 12
           ++ L SFS +S +   G PG   CK  +  L  R      +L+SA +  S+
Sbjct: 12  IFFLCSFSYVSSIKLCGIPGAPACKRREISLYKRATDTFPDLSSAIASTSY 62


>SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 440

 Score = 26.6 bits (56), Expect = 1.7
 Identities = 21/58 (36%), Positives = 25/58 (43%), Gaps = 5/58 (8%)
 Frame = +3

Query: 201 RRWQVNWIRFDLRHTRSGKKFEPKHRLARHGLYEKKRPT-----RKQRKERKNRMKKV 359
           R W     R  + H     +F  KH L RHG  EKKR       R+Q   +  R KKV
Sbjct: 39  RSWNSIVRRSSVWHNLYLSEFGTKH-LRRHGRIEKKRRNWKGLFRRQSNWKDGRCKKV 95


>SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 202

 Score = 25.8 bits (54), Expect = 3.0
 Identities = 10/36 (27%), Positives = 21/36 (58%)
 Frame = +3

Query: 249 SGKKFEPKHRLARHGLYEKKRPTRKQRKERKNRMKK 356
           S KK + +    +     +K   +K++KE+K+++KK
Sbjct: 124 SSKKVKVRKTSGKESSKREKSKKKKEKKEKKDKLKK 159


>SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 639

 Score = 25.8 bits (54), Expect = 3.0
 Identities = 9/37 (24%), Positives = 23/37 (62%), Gaps = 1/37 (2%)
 Frame = +3

Query: 231 DLRHTRSGKKFEPKHRLARHGLYEK-KRPTRKQRKER 338
           +  H+R  +    +HR ++HG +++ +RP+ ++  +R
Sbjct: 21  ETNHSRRHRHHHSRHRESKHGRHDRSERPSSREAGDR 57


>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1085

 Score = 25.0 bits (52), Expect = 5.2
 Identities = 10/42 (23%), Positives = 24/42 (57%)
 Frame = +3

Query: 231 DLRHTRSGKKFEPKHRLARHGLYEKKRPTRKQRKERKNRMKK 356
           + R  R  ++ + +    +  L  +KR  ++++KER+ ++KK
Sbjct: 636 EARKKREEQRLKREQEKKQQELERQKREEKQKQKEREKKLKK 677


>SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1854

 Score = 24.6 bits (51), Expect = 6.9
 Identities = 11/25 (44%), Positives = 16/25 (64%)
 Frame = -2

Query: 206 PPKFVLKPNTNTTSGVTLYILASFS 132
           PPK  LKP+ +T+  ++ Y LA  S
Sbjct: 818 PPKEPLKPSLDTSPELSKYSLAKLS 842


>SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit
           Rpt2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 448

 Score = 24.6 bits (51), Expect = 6.9
 Identities = 8/23 (34%), Positives = 17/23 (73%)
 Frame = +3

Query: 300 EKKRPTRKQRKERKNRMKKVRGT 368
           E+ +P  ++ +E +NR+ ++RGT
Sbjct: 90  ERLKPQDERTQEERNRVDEIRGT 112


>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 817

 Score = 24.6 bits (51), Expect = 6.9
 Identities = 10/46 (21%), Positives = 22/46 (47%)
 Frame = +1

Query: 55  DCWRASSMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFGFK 192
           +C R    VC   +  K  ++++ + E L K+  +  D + ++  K
Sbjct: 640 NCLRVLEKVCKYSNIRKEIIARSNLHENLKKLLAIPHDKLRLYALK 685


>SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 327

 Score = 24.6 bits (51), Expect = 6.9
 Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
 Frame = +3

Query: 249 SGKKFEPKHRLARHGLYEKKRPTRKQRKER-KNRMK 353
           SG+ F  K    R    EKK   ++++KE+ K RMK
Sbjct: 277 SGEYFLKKEEKERKKRAEKKEQQKEKKKEKEKERMK 312


>SPCC1393.02c |||non-specific DNA binding protein Spt2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 406

 Score = 24.6 bits (51), Expect = 6.9
 Identities = 11/36 (30%), Positives = 20/36 (55%)
 Frame = +2

Query: 203 EVASQLDSL*STTHSIWQEVRAQAQVSPPRPVREEE 310
           +V S  D + +T H +W+E +A A+ +      EE+
Sbjct: 356 DVFSDEDDMEATGHDVWREEQAAARAARLEDELEEQ 391


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,885,039
Number of Sequences: 5004
Number of extensions: 38223
Number of successful extensions: 123
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 162176800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)