Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40109
(825 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1) 104 8e-23
At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1) (... 53 3e-07
At1g77440.1 68414.m09018 20S proteasome beta subunit C (PBC2) id... 51 8e-07
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 33 0.30
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 33 0.30
At3g22630.1 68416.m02857 20S proteasome beta subunit D (PBD1) (P... 32 0.40
At5g22760.1 68418.m02658 PHD finger family protein contains Pfam... 30 1.6
At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2) (... 30 1.6
At4g26920.1 68417.m03874 hypothetical protein 29 2.8
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 29 3.7
At1g63990.1 68414.m07248 DNA topoisomerase VIA, putative (SPO11-... 29 3.7
At5g55490.1 68418.m06911 expressed protein 29 5.0
At3g15050.1 68416.m01904 calmodulin-binding family protein simil... 28 8.7
>At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1)
Length = 223
Score = 104 bits (249), Expect = 8e-23
Identities = 43/87 (49%), Positives = 63/87 (72%)
Frame = +1
Query: 238 KAVQIIESTVLGATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRF 417
K ++ + VL ++G D L ++L++R +Y+H+HNK M+ PA+AQ+LS LY+KRF
Sbjct: 48 KIHKLADRAVLSSSGFQADVKALQKVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRF 107
Query: 418 FPYYVSNVLAGLDSDGKGCVYSYDPIG 498
FPYY NVL GLD +GKGCV++YD +G
Sbjct: 108 FPYYAFNVLGGLDEEGKGCVFTYDAVG 134
Score = 66.5 bits (155), Expect = 2e-11
Identities = 31/50 (62%), Positives = 38/50 (76%), Gaps = 1/50 (2%)
Frame = +2
Query: 107 KQH-RFEPYADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKL 253
KQH + PY +NGG+ VAIAG DY VI ADTR+STG+SI +RD K+ KL
Sbjct: 3 KQHANWSPYDNNGGTCVAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKL 52
Score = 66.5 bits (155), Expect = 2e-11
Identities = 36/83 (43%), Positives = 51/83 (61%), Gaps = 4/83 (4%)
Frame = +3
Query: 516 FRAGGSAAAQLQPLLDNQIG----LKNMQNVTEAPLPREKALALLKDVFISAAERDIYTG 683
+ A GS + + P LDNQ+ L + + PL +A+ L+K VF SA ERDIYTG
Sbjct: 141 YGAQGSGSTLIMPFLDNQLKSPSPLLLPKQDSNTPLSEAEAVDLVKTVFASATERDIYTG 200
Query: 684 DSIYILIITANGIQEEKFELRKD 752
D + I+I+ A+GI+ E +LRKD
Sbjct: 201 DKLEIMILKADGIKTELMDLRKD 223
>At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1)
(PRCT) almost identical to GB:AAC32069 from [Arabidopsis
thaliana], EST gb|T76747 comes from this gene; identical
to cDNA proteasome subunit prct GI:2511567
Length = 204
Score = 52.8 bits (121), Expect = 3e-07
Identities = 28/84 (33%), Positives = 43/84 (51%)
Frame = +1
Query: 247 QIIESTVLGATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPY 426
+I + +G +G D TL + L R ++Y+ + M A ++S +LY KRF PY
Sbjct: 44 KIHDRVFIGLSGLATDVQTLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPY 103
Query: 427 YVSNVLAGLDSDGKGCVYSYDPIG 498
V+AGL D K + + D IG
Sbjct: 104 LCQPVIAGLGDDDKPFICTMDSIG 127
>At1g77440.1 68414.m09018 20S proteasome beta subunit C (PBC2)
identical to residues 14-204 of 20S proteasome beta
subunit PBC2 GB:AAC32069 [Arabidopsis thaliana]
Length = 204
Score = 51.2 bits (117), Expect = 8e-07
Identities = 27/84 (32%), Positives = 43/84 (51%)
Frame = +1
Query: 247 QIIESTVLGATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPY 426
+I + +G +G D TL + L R ++Y+ + M A ++S +LY KRF P+
Sbjct: 44 KIHDHLFIGLSGLATDVQTLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPF 103
Query: 427 YVSNVLAGLDSDGKGCVYSYDPIG 498
V+AGL D K + + D IG
Sbjct: 104 LCQPVIAGLGDDNKPFICTMDSIG 127
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 32.7 bits (71), Expect = 0.30
Identities = 21/85 (24%), Positives = 38/85 (44%)
Frame = +1
Query: 226 QRPKKAVQIIESTVLGATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLY 405
Q KK ++I + G D R L + +++E + + ++ +++L+ MLY
Sbjct: 86 QSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLANMLY 145
Query: 406 YKRFFPYYVSNVLAGLDSDGKGCVY 480
R V ++AG D G G Y
Sbjct: 146 SYRGMGLSVGTMIAGWDETGPGLYY 170
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 32.7 bits (71), Expect = 0.30
Identities = 21/85 (24%), Positives = 38/85 (44%)
Frame = +1
Query: 226 QRPKKAVQIIESTVLGATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLY 405
Q KK ++I + G D R L + +++E + + ++ +++L+ MLY
Sbjct: 86 QSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLANMLY 145
Query: 406 YKRFFPYYVSNVLAGLDSDGKGCVY 480
R V ++AG D G G Y
Sbjct: 146 SYRGMGLSVGTMIAGWDETGPGLYY 170
>At3g22630.1 68416.m02857 20S proteasome beta subunit D (PBD1)
(PRGB) identical to GB:CAA74026 from [Arabidopsis
thaliana] ( FEBS Lett. (1997) 416 (3), 281-285);
identical to cDNA proteasome subunit prgb GI:2511589
Length = 204
Score = 32.3 bits (70), Expect = 0.40
Identities = 22/94 (23%), Positives = 45/94 (47%), Gaps = 2/94 (2%)
Frame = +1
Query: 220 IYQRPKKAVQIIES-TVLGATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLST 396
+++ + + +++S ++ A+G D + T +Q + +Y+ + +TT A A
Sbjct: 27 LHKNNEDKIMVLDSHKLVAASGEPGDRVQFTEYVQKNVSLYKFRNGIPLTTAAAANFTRG 86
Query: 397 MLYYK-RFFPYYVSNVLAGLDSDGKGCVYSYDPI 495
L R PY V+ ++AG D + +Y D I
Sbjct: 87 ELATALRKNPYSVNILMAGYDDESGASLYYIDYI 120
>At5g22760.1 68418.m02658 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 1566
Score = 30.3 bits (65), Expect = 1.6
Identities = 12/36 (33%), Positives = 25/36 (69%)
Frame = -2
Query: 362 IDLLCSCSYICILACRRRVKVNVSHQQPVAPNTVLS 255
+++L +C C LA R R ++N++H+QP P+ +++
Sbjct: 1122 LEILVTCKR-CYLA-RARSQININHRQPTTPSVLIN 1155
>At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2)
(PRCGA) identical to SP|O24633 Proteasome subunit beta
type 2-2 (EC 3.4.25.1) (20S proteasome alpha subunit D2)
{Arabidopsis thaliana}, cDNA proteasome subunit prcga
GI:2511571
Length = 199
Score = 30.3 bits (65), Expect = 1.6
Identities = 23/98 (23%), Positives = 42/98 (42%), Gaps = 1/98 (1%)
Frame = +1
Query: 205 HWIFHIYQRPKKAVQIIESTVLGATGCWCDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQ 384
H I + K + + ++ A+G D + T +Q + +Y+ + ++T A A
Sbjct: 23 HSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYVQKNVSLYQFRNGIPLSTAAAAN 82
Query: 385 MLSTMLYYK-RFFPYYVSNVLAGLDSDGKGCVYSYDPI 495
L R PY V+ ++AG D + +Y D I
Sbjct: 83 FTRGELATALRKNPYSVNILMAGYDKEAGASLYYIDYI 120
>At4g26920.1 68417.m03874 hypothetical protein
Length = 461
Score = 29.5 bits (63), Expect = 2.8
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +1
Query: 382 QMLSTMLYYKRFFPYYVSNV 441
+ML+ YY RFFP+Y NV
Sbjct: 93 EMLTLNEYYSRFFPWYAKNV 112
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 29.1 bits (62), Expect = 3.7
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +2
Query: 140 GGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKL 253
G +I+ + + V+GAD+R STG + R K+ +L
Sbjct: 12 GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQL 49
>At1g63990.1 68414.m07248 DNA topoisomerase VIA, putative (SPO11-2)
similar to topoisomerase 6 subunit A (spo11)
[Arabidopsis thaliana] GI:12331186; contains Pfam
profile PF04406: Type IIB DNA topoisomerase; identical
to cDNA putative topoisomerase VIA (SPO11 gene 2)
GI:7270976
Length = 383
Score = 29.1 bits (62), Expect = 3.7
Identities = 12/49 (24%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = -3
Query: 217 GKSSAETSVCSDNGIIIAGD-GYNTTSVVRVGLETMLFRTRHSIFRKVL 74
GK + + S C +G I GD +++R ++ +H+IF +++
Sbjct: 178 GKEAVDCSACGSSGFAITGDLNLLDNTIMRTDARYIIIVEKHAIFHRLV 226
>At5g55490.1 68418.m06911 expressed protein
Length = 537
Score = 28.7 bits (61), Expect = 5.0
Identities = 9/18 (50%), Positives = 15/18 (83%)
Frame = +1
Query: 235 KKAVQIIESTVLGATGCW 288
+KAVQ++E+ ++G T CW
Sbjct: 7 QKAVQVLENKLVGLTSCW 24
>At3g15050.1 68416.m01904 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 259
Score = 27.9 bits (59), Expect = 8.7
Identities = 16/69 (23%), Positives = 31/69 (44%)
Frame = -1
Query: 540 QQRNHQRGSYGRTVSDRVVTIYTSLTVAVQTRQHVGYVVWEKSFVIQHS*EHLCDCRSGH 361
++ RG+ S+RV + +S + + V + +K+F + + LC +S
Sbjct: 17 KKNKSNRGNVHSETSNRVKPVESSSAASTKLTVEVAVIRIQKAFRAFKARKRLCSLKSAR 76
Query: 360 RFVMLVFVH 334
RF L+ H
Sbjct: 77 RFNSLIQGH 85
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,791,039
Number of Sequences: 28952
Number of extensions: 381884
Number of successful extensions: 923
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 897
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 923
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1892353600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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